FastQCFastQC Report
Thu 26 May 2016
SRR936822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960249
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151951.5824020644645296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105661.1003395994164014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74140.772091405458376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31060.3234577698076228No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30430.31689697151468005No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24920.25951602136529167No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23120.24077088338545524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.20171851259412923No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17970.1871389608320342No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.17922434701832546No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12950.13486085379937912No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11000.11455362098788958No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.11205426925724472No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG10470.10903421924938218No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.10726384510684207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG905.676011E-736.9615752
TCACGCA1051.8943756E-631.6813474
ATCACGC1051.8951469E-631.6796953
GTATCAA157900.030.969071
GGTATCA106400.028.2273651
TATCAAC191050.025.471112
GAGTACT95500.025.10391212-13
ATCAACG193550.025.0916963
TCAACGC195450.024.7761274
GTACATG112150.024.661451
CAACGCA196450.024.6283865
AACGCAG200400.024.1404296
TACATGG113700.024.1162782
AGTACTT96800.023.83402812-13
CTTAACG601.7105551E-423.75172620-21
GTACTTT102750.023.3295514-15
ACATGGG112950.023.0971493
ACGCAGA217850.022.0781757
CGCAGAG217700.022.0278918
CACGCAG1302.9297476E-421.935535
Produced by FastQC (version 0.11.5)