##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936822_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 960249 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912460205634165 33.0 31.0 34.0 30.0 34.0 2 31.97203850251341 33.0 31.0 34.0 30.0 34.0 3 31.949645352403387 33.0 31.0 34.0 30.0 34.0 4 35.57172358419535 37.0 35.0 37.0 33.0 37.0 5 35.60284780301776 37.0 35.0 37.0 33.0 37.0 6 35.71515200744807 37.0 35.0 37.0 33.0 37.0 7 35.67391270389243 37.0 35.0 37.0 33.0 37.0 8 35.63040836283089 37.0 35.0 37.0 33.0 37.0 9 37.24890835606181 39.0 37.0 39.0 34.0 39.0 10-11 37.08538358279988 39.0 37.0 39.0 33.0 39.0 12-13 37.017030478552954 39.0 37.0 39.0 33.0 39.0 14-15 38.055208596936836 40.0 38.0 41.0 33.0 41.0 16-17 38.044476484745104 40.0 38.0 41.0 33.0 41.0 18-19 38.1594024050012 40.0 38.0 41.0 33.5 41.0 20-21 38.1554919609393 40.0 38.0 41.0 33.5 41.0 22-23 38.05499823483284 40.0 38.0 41.0 33.0 41.0 24-25 37.900564853491126 40.0 37.5 41.0 33.0 41.0 26-27 37.90890956408181 40.0 37.0 41.0 33.0 41.0 28-29 37.821093799629054 40.0 37.0 41.0 33.0 41.0 30-31 37.560337474967426 40.0 37.0 41.0 33.0 41.0 32-33 37.22871984245753 39.0 35.5 41.0 32.5 41.0 34-35 37.00135693971043 39.0 35.0 41.0 32.0 41.0 36-37 36.763509777151555 39.0 35.0 41.0 31.0 41.0 38-39 36.53663008240571 39.0 35.0 41.0 31.0 41.0 40-41 36.281101047749075 39.0 35.0 41.0 30.0 41.0 42-43 35.954217603975636 39.0 35.0 41.0 30.0 41.0 44-45 35.490412903319864 38.5 35.0 40.0 28.0 41.0 46-47 34.83368584606701 38.0 35.0 40.0 24.0 41.0 48-49 34.2645667946543 38.0 34.0 40.0 21.0 41.0 50-51 33.46799944597703 37.5 33.5 39.5 18.0 40.5 52-53 33.637196706270984 38.0 33.5 39.5 18.0 40.5 54-55 33.9867872812156 38.0 34.0 40.0 16.0 41.0 56-57 33.880056110446354 38.0 34.0 40.0 13.5 41.0 58-59 33.648289141670546 38.0 34.0 40.0 10.5 41.0 60-61 33.36717767995593 38.0 34.0 40.0 8.0 41.0 62-63 33.0467024698802 37.0 33.0 40.0 6.5 41.0 64-65 32.71591430972592 36.5 33.0 40.0 2.0 41.0 66-67 32.35043827173993 36.0 33.0 39.0 2.0 41.0 68-69 31.978317603038377 35.5 32.0 39.0 2.0 41.0 70-71 31.568808715239484 35.0 32.0 39.0 2.0 40.5 72-73 31.18951490707098 35.0 31.5 38.0 2.0 40.0 74-75 30.776895888462263 35.0 31.0 37.0 2.0 39.5 76-77 30.352442439408946 35.0 31.0 37.0 2.0 39.0 78-79 29.91838418993407 35.0 30.5 36.0 2.0 39.0 80-81 29.511249165581013 35.0 30.0 36.0 2.0 37.5 82-83 29.11716023656364 34.0 29.5 35.0 2.0 37.0 84-85 28.775565504363975 34.0 29.0 35.0 2.0 37.0 86-87 28.42984527971391 34.0 29.0 35.0 2.0 36.0 88-89 28.081052414529978 34.0 29.0 35.0 2.0 36.0 90-91 27.72349880083187 34.0 27.5 35.0 2.0 35.5 92-93 27.386748124705154 34.0 27.0 35.0 2.0 35.0 94-95 27.00669461775019 34.0 26.0 35.0 2.0 35.0 96-97 26.563066454638328 34.0 25.0 35.0 2.0 35.0 98-99 26.012654009532945 33.5 24.5 35.0 2.0 35.0 100-101 24.046438996551935 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3313593556687344 1101 2 0.28291443904219804 1101 3 0.23931902447023745 1101 4 0.18265620653955494 1101 5 0.20893391624997548 1101 6 0.09444581652979878 1101 7 -0.02470470119921231 1101 8 0.1124513008202257 1101 9 0.2656064156256761 1101 10-11 0.2631560914567501 1101 12-13 0.23133388915405817 1101 14-15 0.4836991662985781 1101 16-17 0.4555670156143279 1101 18-19 0.3895819930467681 1101 20-21 0.3220965932814934 1101 22-23 0.4227568602986125 1101 24-25 0.5193606644872304 1101 26-27 0.45568715252231584 1101 28-29 0.6443773196792506 1101 30-31 0.454243915920884 1101 32-33 0.526135900691763 1101 34-35 0.5252303366348343 1101 36-37 0.3316491942817521 1101 38-39 0.4148594772027039 1101 40-41 0.3347654114661225 1101 42-43 0.4356190746532107 1101 44-45 0.4151142351327337 1101 46-47 0.6320205208477532 1101 48-49 0.5163994603595441 1101 50-51 0.40748096505681985 1101 52-53 0.6444735173558414 1101 54-55 0.6432414250758143 1101 56-57 0.7646983390530195 1101 58-59 0.8194334099149643 1101 60-61 0.6438454646810925 1101 62-63 0.38840793628779835 1101 64-65 0.6284391720389877 1101 66-67 0.6930848193508865 1101 68-69 0.6353666736307702 1101 70-71 0.654297696785413 1101 72-73 0.7829848871253873 1101 74-75 0.478271660208204 1101 76-77 0.5304221074526687 1101 78-79 0.6057105432096179 1101 80-81 0.5603144212490072 1101 82-83 0.45356124537882536 1101 84-85 0.5222663165321606 1101 86-87 0.6140655101895511 1101 88-89 0.7259894684161559 1101 90-91 0.5937549711057208 1101 92-93 0.5910556279454404 1101 94-95 0.7043642427010148 1101 96-97 0.5573320040837189 1101 98-99 0.779497824445599 1101 100-101 1.0323612211576254 1102 1 0.10426020403231462 1102 2 -8.804803701174535E-4 1102 3 0.12793190205370664 1102 4 0.13226748744521188 1102 5 -0.0333953488670673 1102 6 -0.16390676145386607 1102 7 -0.11558513538593473 1102 8 0.03686094119081673 1102 9 -0.10675272723930362 1102 10-11 0.163157290678285 1102 12-13 0.2709668759694708 1102 14-15 0.2641568234993912 1102 16-17 0.30187307065516933 1102 18-19 0.12145832932219491 1102 20-21 -0.09864085943491574 1102 22-23 -0.008614689369721873 1102 24-25 0.14670342941240477 1102 26-27 0.1382733422871283 1102 28-29 0.1737732375291472 1102 30-31 0.2594727787847617 1102 32-33 0.17432370563665955 1102 34-35 0.09506700266191359 1102 36-37 -0.10619857996307758 1102 38-39 0.11034158139107575 1102 40-41 0.13178902927764824 1102 42-43 0.1869266544185777 1102 44-45 0.1712551059731524 1102 46-47 0.08373900530584422 1102 48-49 0.30451414705435553 1102 50-51 0.26110269974790157 1102 52-53 0.0027782121368318258 1102 54-55 0.07089495969562876 1102 56-57 0.28532023820876873 1102 58-59 0.1801052823452025 1102 60-61 -0.011368065278894335 1102 62-63 -0.08523874221119598 1102 64-65 0.09428277299289078 1102 66-67 -0.26695583425911806 1102 68-69 -0.36333174271153723 1102 70-71 -0.23263284430333897 1102 72-73 -0.10045077977884276 1102 74-75 -0.31787909564399897 1102 76-77 -0.056969020363791145 1102 78-79 0.08954632181276523 1102 80-81 -0.006153717148013271 1102 82-83 -0.09556272329065862 1102 84-85 -0.18627359698683676 1102 86-87 -0.2511265708367105 1102 88-89 -0.3575735729467091 1102 90-91 0.006306451972996285 1102 92-93 0.07441942721631278 1102 94-95 0.12482844422505224 1102 96-97 0.07725064546851357 1102 98-99 0.2556547305910968 1102 100-101 0.1669374333992799 1103 1 0.20067852407179387 1103 2 0.1884339867795468 1103 3 0.20103497788157298 1103 4 0.09957247917940037 1103 5 0.1813378471683933 1103 6 0.12422238996570911 1103 7 0.1522066628014258 1103 8 0.17653909533857615 1103 9 0.0826235324178981 1103 10-11 0.37571335891414037 1103 12-13 0.2621034602658838 1103 14-15 0.25941192074640895 1103 16-17 0.3882268302213703 1103 18-19 0.3657283439577057 1103 20-21 0.5122187082037541 1103 22-23 0.4134442200517796 1103 24-25 0.4477103933433497 1103 26-27 0.444098005623772 1103 28-29 0.42578551476927373 1103 30-31 0.5204784052888343 1103 32-33 0.5828073090371149 1103 34-35 0.3653664762405384 1103 36-37 0.4541765090223606 1103 38-39 0.598857062953627 1103 40-41 0.4032981123530206 1103 42-43 0.24530787694525458 1103 44-45 0.5012232870641355 1103 46-47 0.3546047691247267 1103 48-49 0.40532396749210164 1103 50-51 0.2418031220531418 1103 52-53 0.19112854687445946 1103 54-55 0.2723762022837022 1103 56-57 0.414522446148176 1103 58-59 0.27559375211723847 1103 60-61 0.21590562389463486 1103 62-63 0.2552197751241536 1103 64-65 0.36830884265928177 1103 66-67 0.5704452236624746 1103 68-69 0.4883091023052373 1103 70-71 0.14462734363052832 1103 72-73 0.045112899093116 1103 74-75 -0.1974772411915957 1103 76-77 -0.1010330035426854 1103 78-79 0.08633268818477546 1103 80-81 0.03371218464591763 1103 82-83 0.02496483235857383 1103 84-85 -0.08115946283953335 1103 86-87 -0.06652399400449838 1103 88-89 -0.17185365214269765 1103 90-91 -0.01642853519870968 1103 92-93 0.08109852010230512 1103 94-95 0.19936544481673835 1103 96-97 0.26899745193861335 1103 98-99 0.2902509165543776 1103 100-101 0.1945045100126741 1104 1 0.15612206882765278 1104 2 0.16180354780184913 1104 3 0.2032451756672593 1104 4 0.20167126652955858 1104 5 -0.0024327656919211904 1104 6 0.10557850969204452 1104 7 0.21829646527304192 1104 8 0.29569680157010225 1104 9 -0.007840197438817142 1104 10-11 0.25615812583990305 1104 12-13 0.3412022965028356 1104 14-15 0.45807756850932435 1104 16-17 0.3906004128259113 1104 18-19 0.4231857674177135 1104 20-21 0.28117199043752095 1104 22-23 0.35413939029361075 1104 24-25 0.29499962049455775 1104 26-27 0.30271481805028344 1104 28-29 0.08361010379856282 1104 30-31 0.29376588795332026 1104 32-33 0.3370996983921444 1104 34-35 0.14742785232666478 1104 36-37 0.12469236764174241 1104 38-39 0.1817034580670125 1104 40-41 0.39220852858549193 1104 42-43 0.226455584540453 1104 44-45 0.20440195801225514 1104 46-47 0.19340699115826254 1104 48-49 0.42916399960301277 1104 50-51 0.4826555019395613 1104 52-53 0.5890040296315746 1104 54-55 0.6609181664522552 1104 56-57 0.5085253227501809 1104 58-59 0.4215439956506728 1104 60-61 0.6174193134840706 1104 62-63 0.41507059320608874 1104 64-65 0.5736488245968943 1104 66-67 0.503592529871355 1104 68-69 0.49778413530382437 1104 70-71 0.4650236868981743 1104 72-73 0.6864870812041524 1104 74-75 0.615991328457941 1104 76-77 0.5027639567277866 1104 78-79 0.5702064779149474 1104 80-81 0.6896005992471181 1104 82-83 0.6949467957037712 1104 84-85 0.6443540414946654 1104 86-87 0.6503146769950803 1104 88-89 0.7321442585900151 1104 90-91 0.7190218537086217 1104 92-93 0.683081522935165 1104 94-95 0.7659685586483569 1104 96-97 0.9815881176481263 1104 98-99 0.7490020390209509 1104 100-101 0.7154023288896703 1105 1 0.20381173427118782 1105 2 0.27089541163257636 1105 3 0.22152885471105677 1105 4 0.286010677522583 1105 5 0.40255049878903293 1105 6 0.3431160480452178 1105 7 0.4110152243548981 1105 8 0.40648258783167535 1105 9 0.35225638601651355 1105 10-11 0.5331502048739338 1105 12-13 0.4552826758835167 1105 14-15 0.5122640738052056 1105 16-17 0.43040804262916055 1105 18-19 0.32717540289393554 1105 20-21 0.4550267368154195 1105 22-23 0.5449188752723799 1105 24-25 0.5557138383070921 1105 26-27 0.4099009059767198 1105 28-29 0.4575369165716978 1105 30-31 0.34641238146146236 1105 32-33 0.5673789918879706 1105 34-35 0.5049084075276227 1105 36-37 0.4832005027245856 1105 38-39 0.49947778341780946 1105 40-41 0.6829140459199081 1105 42-43 0.849290310765106 1105 44-45 1.0714109400563174 1105 46-47 0.9278803496786239 1105 48-49 1.0762663601395914 1105 50-51 1.0774793964763774 1105 52-53 0.9669881985638895 1105 54-55 1.0398058813952602 1105 56-57 1.2046618034656333 1105 58-59 1.1319959768880636 1105 60-61 1.162894023702016 1105 62-63 1.0560314664045407 1105 64-65 1.1107503431517287 1105 66-67 0.9808404627156122 1105 68-69 0.9457116379587802 1105 70-71 1.0301084237607654 1105 72-73 1.4083162277153143 1105 74-75 1.334159212376722 1105 76-77 1.302893784882027 1105 78-79 1.184298626933039 1105 80-81 1.4649539826104636 1105 82-83 1.4826791215561421 1105 84-85 1.314893371548795 1105 86-87 1.3134391354785926 1105 88-89 1.2494895353892552 1105 90-91 1.363173204002905 1105 92-93 1.2372186862895838 1105 94-95 1.0777815626491254 1105 96-97 1.1705804284534658 1105 98-99 1.158451880900401 1105 100-101 1.0963121580760138 1106 1 0.09923424210980158 1106 2 0.0889877129463521 1106 3 0.16750719162899586 1106 4 -0.045238836612838895 1106 5 -0.055030284295106924 1106 6 -0.09777042807615288 1106 7 0.13155239623435477 1106 8 0.13924411253950808 1106 9 0.02512052532360798 1106 10-11 0.17222732130004204 1106 12-13 0.20815899419607575 1106 14-15 0.22574649198561048 1106 16-17 0.20707876896431543 1106 18-19 0.04455445500452271 1106 20-21 0.295264745677585 1106 22-23 0.15153375957182647 1106 24-25 0.2288662572648832 1106 26-27 0.22213368045781579 1106 28-29 0.06925985715370331 1106 30-31 0.15627413679301583 1106 32-33 0.18725474011942111 1106 34-35 0.06677516402525185 1106 36-37 0.1742068254423259 1106 38-39 0.172750048971956 1106 40-41 0.17808779109365247 1106 42-43 0.33053269888669234 1106 44-45 0.49356571707686925 1106 46-47 0.20464500983253942 1106 48-49 0.5908941230895053 1106 50-51 0.5234512551999089 1106 52-53 0.5455654575102784 1106 54-55 0.7511472563272363 1106 56-57 0.7156391038724621 1106 58-59 0.5777054687384933 1106 60-61 0.6671534365184755 1106 62-63 0.7393352159834592 1106 64-65 0.6362711899813078 1106 66-67 0.6511975407908039 1106 68-69 0.8155226042462616 1106 70-71 0.7933790049843736 1106 72-73 0.7710044192625638 1106 74-75 0.6476714302513535 1106 76-77 0.8204108225332938 1106 78-79 0.6943592628326023 1106 80-81 0.8283223845694714 1106 82-83 0.7219382752448276 1106 84-85 0.6429102666797881 1106 86-87 0.8894672249295006 1106 88-89 1.0195233515640076 1106 90-91 0.796214586708718 1106 92-93 0.9745991636029459 1106 94-95 0.9249382831279966 1106 96-97 0.9046537875268257 1106 98-99 0.7212385410024922 1106 100-101 0.5173235337853797 1107 1 0.10048870684741118 1107 2 0.09438516497301563 1107 3 0.14222990792474732 1107 4 0.07551875886385773 1107 5 0.09467760979480744 1107 6 0.038000977358173316 1107 7 0.018035990583115336 1107 8 0.0998315057111725 1107 9 0.3030079633234024 1107 10-11 0.23297667418422208 1107 12-13 0.3114227716171527 1107 14-15 0.14091647738031554 1107 16-17 0.21207312929576005 1107 18-19 0.40587825874406036 1107 20-21 0.40705129145289476 1107 22-23 0.1969620128652494 1107 24-25 0.37190596631582906 1107 26-27 0.13457601846194933 1107 28-29 0.33887397367792715 1107 30-31 0.16290255493828454 1107 32-33 0.3004873547565339 1107 34-35 0.3381950590406504 1107 36-37 0.24309031567413086 1107 38-39 0.1492568527259479 1107 40-41 0.5483501079851791 1107 42-43 0.3491996403064448 1107 44-45 0.43988221816864836 1107 46-47 0.17424141201320964 1107 48-49 0.23318729909054525 1107 50-51 -0.021593789845141487 1107 52-53 0.1386507177303713 1107 54-55 0.1170207542232049 1107 56-57 0.42512567712308424 1107 58-59 0.4910949818471053 1107 60-61 0.4092398246722766 1107 62-63 0.3831359187729291 1107 64-65 0.23936324672504838 1107 66-67 0.12192549185584056 1107 68-69 0.2639829024256599 1107 70-71 0.07448936895990244 1107 72-73 0.20224022971185818 1107 74-75 0.3156377513075661 1107 76-77 0.2438794356086511 1107 78-79 0.40974398945328616 1107 80-81 0.2568558538332759 1107 82-83 0.22889205888400355 1107 84-85 0.047686632130311324 1107 86-87 0.02342742911442386 1107 88-89 0.14416906469286772 1107 90-91 0.255793346849277 1107 92-93 0.4228588197277716 1107 94-95 0.35187746147166976 1107 96-97 0.30467620159418374 1107 98-99 0.21634721307745863 1107 100-101 0.3195338500406386 1108 1 0.0876082889944847 1108 2 -0.06084089432906481 1108 3 0.09031885380235494 1108 4 0.11530461648380452 1108 5 0.053354143238259155 1108 6 -0.18849841774031262 1108 7 -0.12030715881668641 1108 8 -0.06819709490169856 1108 9 -0.03240992582386326 1108 10-11 -0.054131498143888734 1108 12-13 0.1490266371913762 1108 14-15 0.2286235746499301 1108 16-17 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2316 62-63 -1.0634636412779699 2316 64-65 -1.4005686042961116 2316 66-67 -1.109646247884072 2316 68-69 -1.1115787156093795 2316 70-71 -1.0625364457007542 2316 72-73 -1.317726414669803 2316 74-75 -1.363565128490002 2316 76-77 -1.7474698494740544 2316 78-79 -1.9207983268931237 2316 80-81 -1.8835585394035839 2316 82-83 -1.6617721420548435 2316 84-85 -1.8007735460059457 2316 86-87 -2.277626795331507 2316 88-89 -2.3064804353322934 2316 90-91 -2.0351499557882633 2316 92-93 -1.8726762719642345 2316 94-95 -2.559022061916803 2316 96-97 -2.7597732869290397 2316 98-99 -2.483096380702868 2316 100-101 -2.509710903997618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1703.0 3 554.0 4 525.0 5 556.0 6 622.0 7 792.0 8 1027.0 9 1340.0 10 1890.0 11 2636.0 12 4100.0 13 5881.0 14 6698.0 15 9360.0 16 12768.0 17 18846.0 18 15587.0 19 7689.0 20 6483.0 21 6116.0 22 6624.0 23 6974.0 24 7818.0 25 8361.0 26 9827.0 27 11624.0 28 13816.0 29 16441.0 30 19985.0 31 24994.0 32 31931.0 33 41741.0 34 58298.0 35 88484.0 36 126811.0 37 180660.0 38 168661.0 39 32017.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.787334371021416 19.45290083827694 15.08815139410685 18.6716133965948 2 15.177403978891766 22.163541932559923 40.960185035657915 21.6988690528904 3 19.03920132750968 29.648653688099806 30.982517580857362 20.329627403533152 4 11.716128978482748 17.87873988839202 38.64395053907738 31.76118059404785 5 11.802907636531886 40.29778733257538 32.26078067050086 15.638524360391868 6 27.200585245681257 38.15268284498597 18.474213654273616 16.172518255059153 7 25.622278103209844 37.51061663500347 20.435315904162138 16.43178935762454 8 27.069295044279883 32.530966737461675 19.892390196523888 20.507348021734554 9 29.13515558320646 16.289784208348834 20.638339778841434 33.93672042960327 10-11 24.08428172419543 28.526971819242952 26.064855344338838 21.32389111222278 12-13 27.215904235251447 27.008039453142096 27.166243884950568 18.609812426655882 14-15 23.987498684318854 26.17574999036036 28.798045809672786 21.038705515648008 16-17 19.507835269183357 30.41078504822866 28.557235133099024 21.524144549488955 18-19 19.405580561807117 27.224149787446862 34.259867050095856 19.11040260065016 20-21 21.892669863838012 25.553022942005466 33.87122571838669 18.683081475769836 22-23 20.813412207016853 26.40490493332076 33.65641194189377 19.12527091776861 24-25 19.988698740716824 26.460987219681897 34.492797400189566 19.05751663941171 26-27 20.04867145569627 26.515475814775584 34.31884438361815 19.117008345910005 28-29 19.466873317834494 26.762107425276437 34.53855544167521 19.23246381521386 30-31 19.544240434574526 26.88927275634132 34.420429293889306 19.14605751519485 32-33 19.24884291564428 27.186951921114133 33.9970859387693 19.567119224472282 34-35 19.745943696840516 26.878746518091347 33.92489544509846 19.450414339969683 36-37 20.21226960385089 26.699083726830203 33.73151675598889 19.357129913330017 38-39 20.12966555140406 26.801869475219036 33.79490450835145 19.27356046502545 40-41 19.785422073729073 27.059908515628578 33.76537758132986 19.38929182931249 42-43 19.648320152802853 27.58517441572853 32.990398269511864 19.776107161956755 44-45 19.752903207665657 27.85485992433071 32.354975000807094 20.03726186719654 46-47 20.149956311776656 27.982020655833654 31.4867638742654 20.38125915812429 48-49 20.394474092688238 28.32384178755218 30.778487264388453 20.50319685537113 50-51 20.696577362277196 28.744022608249175 30.372153985630828 20.187246043842798 52-53 20.471418342771592 29.263347002538566 30.085165657749137 20.1800689969407 54-55 19.89148762663162 29.079316410582745 30.202298141885738 20.826897820899905 56-57 20.530762263734285 29.5549915600321 29.31556834238614 20.59867783384747 58-59 20.711797662994886 28.825024566205336 29.694462540377366 20.768715230422412 60-61 20.52089968183965 28.779475662998976 30.24965874190993 20.44996591325144 62-63 20.962592131802182 28.78777384360365 30.145957918166282 20.103676106427887 64-65 20.610718029554803 29.127108983662424 30.12403810452237 20.1381348822604 66-67 20.38974200866394 29.33559080526336 29.867374392102253 20.40729279397045 68-69 20.439684869419565 29.524116572897647 29.714119937540563 20.322078620142232 70-71 20.385948246499407 29.77393909281903 29.355888385096208 20.48422427558536 72-73 20.340767883908104 29.69766693366192 29.145830447250038 20.81573473517994 74-75 20.293096112172098 30.120341374319636 29.121185591949672 20.46537692155859 76-77 20.440606002525012 29.863093179554916 29.22460093583723 20.47169988208284 78-79 20.18579299301844 29.762351863842095 29.27901296560892 20.77284217753055 80-81 20.017094738289558 30.355661186662353 29.016156663223757 20.61108741182433 82-83 19.998249911584075 30.264810253434675 28.81666417522135 20.920275659759895 84-85 20.182741338821963 29.754683501979855 28.753116388754492 21.309458770443694 86-87 20.42055274044699 29.854317480704395 28.706503187244564 21.01862659160405 88-89 20.52026929552877 29.47876722352393 28.721210798694457 21.279752682252838 90-91 19.91439228311896 29.74292686940735 29.19640820475858 21.146272642715118 92-93 20.011407052058345 30.20467584960401 28.855049704015645 20.928867394321994 94-95 19.783492402442675 30.063358550018883 28.740459108605354 21.412689938933084 96-97 19.93914788283389 29.922864393854155 28.392289356453176 21.745698366858775 98-99 19.990820148367657 29.834465706064485 28.425707544097932 21.749006601469922 100-101 20.091464097710375 29.82279808026477 28.797904151836445 21.287833670188405 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2221.0 1 1555.0 2 1032.5 3 1193.5 4 1076.0 5 1213.5 6 2120.5 7 4121.0 8 6467.5 9 7193.0 10 5846.0 11 4591.5 12 4483.0 13 4541.5 14 4417.0 15 4223.0 16 4138.0 17 4169.0 18 4243.5 19 4330.5 20 4439.0 21 4721.0 22 5307.5 23 5948.5 24 6637.0 25 7717.5 26 9112.5 27 10295.5 28 11181.0 29 12354.5 30 13904.5 31 15601.5 32 17856.5 33 19804.5 34 21726.5 35 23014.0 36 24703.0 37 27137.0 38 27262.0 39 26664.5 40 26946.5 41 27565.0 42 27737.5 43 28527.5 44 30228.5 45 31444.5 46 31246.5 47 32150.5 48 41534.5 49 41342.5 50 31010.0 51 26835.0 52 23983.5 53 23149.5 54 22350.0 55 20485.0 56 18975.0 57 17508.5 58 16341.5 59 15334.5 60 13609.0 61 11686.5 62 9669.5 63 6785.5 64 4395.0 65 3236.5 66 2378.5 67 1750.5 68 1205.0 69 855.5 70 646.0 71 449.0 72 320.0 73 303.5 74 259.5 75 190.5 76 141.5 77 78.0 78 40.5 79 20.5 80 14.0 81 15.5 82 11.0 83 6.5 84 5.5 85 3.5 86 2.5 87 4.0 88 4.5 89 3.0 90 2.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04405107425261573 2 0.045196610462494624 3 0.026659751793545215 4 0.0480083811594701 5 0.06644110017297596 6 0.06789905534918547 7 0.06977356914716912 8 0.06987770880261265 9 0.06935701052539497 10-11 0.07591780881833775 12-13 0.07612608812922482 14-15 0.06977356914716912 16-17 0.05727681049394479 18-19 0.050924291511889104 20-21 0.05691232169989242 22-23 0.033064340603322674 24-25 0.018640998324392942 26-27 0.014371272451207966 28-29 0.01843271901350587 30-31 0.014319202623486198 32-33 0.00801875346915227 34-35 0.01119501296018012 36-37 0.00572768104939448 38-39 0.009008080195865863 40-41 0.00885187071270056 42-43 0.0059880301880033205 44-45 0.001874513797983648 46-47 0.003749027595967296 48-49 8.851870712700559E-4 50-51 0.007133566397882215 52-53 0.006404588809777464 54-55 0.008539451746369952 56-57 0.024316609546065657 58-59 0.009216359506752935 60-61 0.020671721605541895 62-63 0.014944040556147417 64-65 0.010466035372075367 66-67 0.018536858668949406 68-69 0.027701148347980573 70-71 0.020619651777820127 72-73 0.018953417290723553 74-75 0.019630325051106536 76-77 0.026347332827214607 78-79 0.03785476475372534 80-81 0.03171052508255671 82-83 0.03098154749445196 84-85 0.021192419882759578 86-87 0.026086983688605767 88-89 0.019630325051106536 90-91 0.01791202073628819 92-93 0.03296020094787914 94-95 0.024629028512396265 96-97 0.039677208723987216 98-99 0.05607920445634413 100-101 0.06638903034525419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 960249.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.843207377073426 #Duplication Level Percentage of deduplicated Percentage of total 1 76.25851751079853 38.772276200697156 2 13.033868112210476 13.253673187090836 3 4.416432159562906 6.736367284662993 4 1.9982486497248004 4.0638948195566 5 1.098533915402123 2.7926493835769293 6 0.6837269526112971 2.0857722744546385 7 0.4570101474119878 1.6265103188806216 8 0.33409672614940944 1.3589239305292589 9 0.24434785506323142 1.1181085800410664 >10 1.3038301239218013 12.210382914830872 >50 0.10678580380221309 3.736984592580819 >100 0.05781265667604189 5.210863295908008 >500 0.0037033018173717133 1.2666534840415844 >1k 0.0024688678782478087 2.2966920386173117 >5k 2.0573898985398403E-4 0.7755362895932205 >10k+ 4.1147797970796806E-4 2.6947114049380843 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15195 1.5824020644645296 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10566 1.1003395994164014 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7414 0.772091405458376 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3106 0.3234577698076228 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3043 0.31689697151468005 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2492 0.25951602136529167 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2312 0.24077088338545524 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1937 0.20171851259412923 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1797 0.1871389608320342 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.17922434701832546 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1295 0.13486085379937912 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1100 0.11455362098788958 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1076 0.11205426925724472 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1047 0.10903421924938218 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1030 0.10726384510684207 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.04139655443536E-4 0.0 0.0 0.0 6 0.0 1.04139655443536E-4 0.0 0.0 0.0 7 0.0 1.04139655443536E-4 0.0 0.0 0.0 8 0.0 1.04139655443536E-4 0.0 0.0 0.0 9 0.0 1.04139655443536E-4 0.0 0.0 0.0 10-11 0.0 1.04139655443536E-4 0.0 0.0 0.0 12-13 0.0 2.08279310887072E-4 0.0 0.0 5.2069827721768E-5 14-15 0.0 2.08279310887072E-4 0.0 0.0 1.04139655443536E-4 16-17 0.0 2.6034913860884E-4 0.0 1.04139655443536E-4 1.04139655443536E-4 18-19 0.0 3.12418966330608E-4 0.0 1.56209483165304E-4 1.04139655443536E-4 20-21 0.0 3.12418966330608E-4 0.0 3.64488794052376E-4 1.56209483165304E-4 22-23 0.0 3.12418966330608E-4 0.0 6.76907760382984E-4 2.08279310887072E-4 24-25 0.0 3.12418966330608E-4 0.0 0.001353815520765968 2.08279310887072E-4 26-27 0.0 3.12418966330608E-4 0.0 0.002239002592036024 2.08279310887072E-4 28-29 0.0 3.12418966330608E-4 0.0 0.0052069827721768 2.08279310887072E-4 30-31 0.0 3.12418966330608E-4 0.0 0.00859152157409172 2.08279310887072E-4 32-33 0.0 3.12418966330608E-4 0.0 0.013902644001712056 2.08279310887072E-4 34-35 0.0 3.12418966330608E-4 0.0 0.01843271901350587 2.08279310887072E-4 36-37 0.0 3.12418966330608E-4 0.0 0.02525386644505748 2.08279310887072E-4 38-39 0.0 4.16558621774144E-4 0.0 0.03452229577953218 2.08279310887072E-4 40-41 0.0 4.16558621774144E-4 0.0 0.052694665654429214 2.08279310887072E-4 42-43 0.0 4.16558621774144E-4 0.0 0.06925287086995144 2.08279310887072E-4 44-45 0.0 4.16558621774144E-4 0.0 0.0872169614339614 2.08279310887072E-4 46-47 0.0 4.16558621774144E-4 0.0 0.12434274859958198 2.08279310887072E-4 48-49 0.0 4.16558621774144E-4 0.0 0.14829486935159525 2.08279310887072E-4 50-51 0.0 4.16558621774144E-4 0.0 0.1755794590778017 2.6034913860884E-4 52-53 0.0 4.16558621774144E-4 0.0 0.2059882384673142 4.16558621774144E-4 54-55 0.0 4.16558621774144E-4 0.0 0.2487896368546075 4.16558621774144E-4 56-57 0.0 4.16558621774144E-4 0.0 0.3631870483593318 4.16558621774144E-4 58-59 0.0 4.16558621774144E-4 0.0 0.4539447580783734 4.16558621774144E-4 60-61 0.0 4.16558621774144E-4 0.0 0.5194486013523576 4.16558621774144E-4 62-63 0.0 4.16558621774144E-4 0.0 0.6020834179468033 4.16558621774144E-4 64-65 0.0 4.16558621774144E-4 0.0 0.671075939678146 4.16558621774144E-4 66-67 0.0 4.16558621774144E-4 0.0 0.7956790374163367 4.16558621774144E-4 68-69 0.0 4.6862844949591197E-4 0.0 0.9635521619913168 4.16558621774144E-4 70-71 0.0 5.72768104939448E-4 0.0 1.0357730130414091 4.6862844949591197E-4 72-73 0.0 6.24837932661216E-4 0.0 1.1225734158535963 5.2069827721768E-4 74-75 0.0 6.24837932661216E-4 0.0 1.2970594085492408 5.2069827721768E-4 76-77 0.0 6.24837932661216E-4 0.0 1.481282459028856 5.2069827721768E-4 78-79 0.0 6.24837932661216E-4 0.0 1.659257130181859 5.2069827721768E-4 80-81 0.0 6.24837932661216E-4 0.0 1.8084371866047244 5.2069827721768E-4 82-83 0.0 6.24837932661216E-4 0.0 2.0416579449705234 5.2069827721768E-4 84-85 0.0 6.24837932661216E-4 0.0 2.462590432273296 5.2069827721768E-4 86-87 0.0 7.28977588104752E-4 0.0 2.8132286521516816 5.2069827721768E-4 88-89 0.0 7.810474158265199E-4 0.0 3.24848034207794 5.2069827721768E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 90 5.676011E-7 36.961575 2 TCACGCA 105 1.8943756E-6 31.681347 4 ATCACGC 105 1.8951469E-6 31.679695 3 GTATCAA 15790 0.0 30.96907 1 GGTATCA 10640 0.0 28.227365 1 TATCAAC 19105 0.0 25.47111 2 GAGTACT 9550 0.0 25.103912 12-13 ATCAACG 19355 0.0 25.091696 3 TCAACGC 19545 0.0 24.776127 4 GTACATG 11215 0.0 24.66145 1 CAACGCA 19645 0.0 24.628386 5 AACGCAG 20040 0.0 24.140429 6 TACATGG 11370 0.0 24.116278 2 AGTACTT 9680 0.0 23.834028 12-13 CTTAACG 60 1.7105551E-4 23.751726 20-21 GTACTTT 10275 0.0 23.32955 14-15 ACATGGG 11295 0.0 23.097149 3 ACGCAGA 21785 0.0 22.078175 7 CGCAGAG 21770 0.0 22.027891 8 CACGCAG 130 2.9297476E-4 21.93553 5 >>END_MODULE