FastQCFastQC Report
Thu 26 May 2016
SRR936822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960249
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186921.946578439550575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145091.510962260830264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105451.098152666652087No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.3762565751174956No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.3301227077560091No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.3222080939423004No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29640.3086699387346407No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28230.2939862473171021No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23680.24660270409029325No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.16495721422256102No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.16006265041671483No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15230.15860469524050533No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15100.15725087971973936No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13380.13933885898345116No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11790.12278065376792895No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG10790.11236668822357533No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10770.11215840891268826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC502.8300346E-547.4903689
GCGTATC550.002576053134.554641
CCCGTAC702.054272E-433.925223
GGTATCA112050.031.8447021
GTATCAA168250.031.289151
GGATACG908.95707E-426.3835378
TATCAAC207100.025.3238772
CATATAG2451.0913936E-1125.2015933
ATCAACG209800.024.9927673
TAACGCA1151.2823974E-424.7801634
TCAACGC211700.024.7684574
CAACGCA213650.024.5868555
AACGCAG216900.024.237826
GAGTACT123100.024.07310312-13
AGTACTT125350.023.09164612-13
GTACATG101800.022.869541
GTACTTT131400.022.51636114-15
ATGGGAG26800.022.3298855
TACATGG103400.022.1905382
ACGCAGA238150.022.015277
Produced by FastQC (version 0.11.5)