FastQCFastQC Report
Thu 26 May 2016
SRR936821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989709
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153511.5510619788240787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106951.080620667287051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73290.7405206985083495No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30590.3090807499982318No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28790.29089358589241887No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25100.2536098994755024No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22770.23006762593853347No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.20056400416688136No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18410.18601427288223105No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.18318515846577124No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12370.1249862333271699No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11080.11195209905133731No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG10970.11084066124487098No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.10811258662899903No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.102757477197843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA702.0481588E-433.9425666
GTATCAA155250.030.3926371
CACGGTA350.008391648527.14375966-67
GGTATCA105600.026.5484311
GTACATG109900.025.4664481
TACATGG110250.025.1726472
TATCAAC188050.025.1698512
ATCAACG189350.024.9719493
ATGGGAG29750.024.9190965
TCAACGC192000.024.6025334
CAACGCA194450.024.268115
ACATGGG109550.024.0754973
AACGCAG198000.023.8557956
GTACGTG1000.001650004623.7585951
GAGTACT92850.023.741612-13
AGTACTT94550.023.49059512-13
CATGGGG21100.023.1979394
CTTTGCG1652.4195597E-623.0409682
GTATAGG3100.022.992191
CATGGGA84850.022.5708474
Produced by FastQC (version 0.11.5)