##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936821_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 989709 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.883034306043495 33.0 31.0 34.0 30.0 34.0 2 31.950585475124505 33.0 31.0 34.0 30.0 34.0 3 31.916413814565697 33.0 31.0 34.0 30.0 34.0 4 35.53170174263344 37.0 35.0 37.0 33.0 37.0 5 35.54970602470019 37.0 35.0 37.0 33.0 37.0 6 35.70056147817186 37.0 35.0 37.0 33.0 37.0 7 35.660516374004885 37.0 35.0 37.0 33.0 37.0 8 35.62907986084799 37.0 35.0 37.0 33.0 37.0 9 37.20536036350079 39.0 37.0 39.0 34.0 39.0 10-11 37.10000666862684 39.0 37.0 39.0 33.0 39.0 12-13 36.99439835345541 39.0 37.0 39.0 33.0 39.0 14-15 38.0398420141678 40.0 38.0 41.0 33.0 41.0 16-17 38.014181441211505 40.0 38.0 41.0 33.0 41.0 18-19 38.11328734001611 40.0 38.0 41.0 33.0 41.0 20-21 38.07440065716286 40.0 38.0 41.0 33.0 41.0 22-23 37.94638019862404 40.0 38.0 41.0 33.0 41.0 24-25 38.00757849024309 40.0 38.0 41.0 33.0 41.0 26-27 37.91850634883587 40.0 37.5 41.0 33.0 41.0 28-29 37.802985018828764 40.0 37.0 41.0 33.0 41.0 30-31 37.50998828948711 39.5 37.0 41.0 33.0 41.0 32-33 37.179841751464316 39.0 35.5 41.0 32.0 41.0 34-35 36.93966761947199 39.0 35.0 41.0 31.5 41.0 36-37 36.688922703542154 39.0 35.0 41.0 31.0 41.0 38-39 36.46262941935458 39.0 35.0 41.0 30.5 41.0 40-41 36.19582725831532 39.0 35.0 41.0 30.0 41.0 42-43 35.85267639275787 39.0 35.0 40.0 30.0 41.0 44-45 35.374138761999745 38.0 35.0 40.0 27.5 41.0 46-47 34.71313891254904 38.0 35.0 40.0 23.5 41.0 48-49 34.18777691220349 38.0 34.0 40.0 21.0 41.0 50-51 33.35832906440176 37.5 33.5 39.5 16.5 40.5 52-53 33.50248861028848 38.0 33.5 39.5 16.0 40.5 54-55 33.88079526406247 38.0 34.0 40.0 14.5 41.0 56-57 33.790507613854174 38.0 34.0 40.0 13.5 41.0 58-59 33.54849102109812 38.0 34.0 40.0 9.0 41.0 60-61 33.27475298294751 37.5 33.5 40.0 8.0 41.0 62-63 32.94532635350391 37.0 33.0 40.0 6.5 41.0 64-65 32.59426508195843 36.5 33.0 40.0 2.0 41.0 66-67 32.21781099292822 36.0 32.5 39.0 2.0 41.0 68-69 31.832872086643647 35.5 32.0 39.0 2.0 41.0 70-71 31.435687156527827 35.0 31.5 38.5 2.0 40.0 72-73 31.046872363492703 35.0 31.0 37.5 2.0 40.0 74-75 30.610499146718883 35.0 31.0 37.0 2.0 39.0 76-77 30.181404837179414 35.0 30.5 36.5 2.0 39.0 78-79 29.76286464001035 35.0 30.0 36.0 2.0 39.0 80-81 29.343235738989947 34.5 29.5 36.0 2.0 37.5 82-83 28.947219839366923 34.0 29.0 35.0 2.0 37.0 84-85 28.56716570224177 34.0 29.0 35.0 2.0 36.5 86-87 28.20290155995348 34.0 29.0 35.0 2.0 36.0 88-89 27.831982936398475 34.0 27.5 35.0 2.0 36.0 90-91 27.444245227637616 34.0 27.0 35.0 2.0 35.5 92-93 27.123976340520294 34.0 26.0 35.0 2.0 35.0 94-95 26.76693906996905 34.0 25.0 35.0 2.0 35.0 96-97 26.28229813005641 33.5 24.5 35.0 2.0 35.0 98-99 25.689196016202743 33.0 21.5 35.0 2.0 35.0 100-101 23.42207355899562 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34197694676957013 1101 2 0.37225657383223165 1101 3 0.14725523694474063 1101 4 0.21673358145943666 1101 5 0.16966392355600846 1101 6 0.07765782139870936 1101 7 0.2896411719681282 1101 8 0.28540182633228284 1101 9 0.3443577283135326 1101 10-11 0.323297673397299 1101 12-13 0.4549383388980175 1101 14-15 0.4381107047912991 1101 16-17 0.30979429990677687 1101 18-19 0.4225618729791947 1101 20-21 0.3807749238823632 1101 22-23 0.31336343383920706 1101 24-25 0.2780911258962604 1101 26-27 0.26858910754362597 1101 28-29 0.1911717750025943 1101 30-31 0.441450682521328 1101 32-33 0.5083689097083308 1101 34-35 0.3958630906602991 1101 36-37 0.4439169598928743 1101 38-39 0.5819063162135976 1101 40-41 0.665518545000289 1101 42-43 0.9171598120832769 1101 44-45 0.9247346238214149 1101 46-47 0.9618263227165258 1101 48-49 0.6998286700598157 1101 50-51 0.9509368159507972 1101 52-53 0.944950270746574 1101 54-55 0.9822989704268892 1101 56-57 0.8022959758503134 1101 58-59 0.66262741991018 1101 60-61 0.8300583852879697 1101 62-63 0.878229440671447 1101 64-65 1.0250610637483817 1101 66-67 0.9672292190894609 1101 68-69 0.8374727440244882 1101 70-71 0.5715483983150769 1101 72-73 0.6927150445340367 1101 74-75 0.7648752165268213 1101 76-77 0.7535834616970654 1101 78-79 0.9050767264958282 1101 80-81 0.9252196447246774 1101 82-83 1.0283915445567224 1101 84-85 1.0854977924405844 1101 86-87 0.9396044965774131 1101 88-89 0.9114403587516904 1101 90-91 0.7627760144584848 1101 92-93 0.9320356813442849 1101 94-95 0.9323817139211421 1101 96-97 1.0442589001774962 1101 98-99 0.750544299415143 1101 100-101 0.7403951769751522 1102 1 0.13421255302930035 1102 2 0.18438858286269522 1102 3 0.10287374497118051 1102 4 0.16437919149808522 1102 5 0.17660138024668726 1102 6 0.12240481530022151 1102 7 0.12128372701245382 1102 8 0.28793467504368664 1102 9 0.3824681629953304 1102 10-11 0.3164124312677572 1102 12-13 0.1756634487034674 1102 14-15 0.2702395632482393 1102 16-17 0.4059885663939653 1102 18-19 0.2809692580651202 1102 20-21 0.2172097136479536 1102 22-23 0.2876061678290327 1102 24-25 -0.014379445888586417 1102 26-27 0.18803929706477618 1102 28-29 0.014195387489131406 1102 30-31 0.21283807651028042 1102 32-33 0.23756959327658222 1102 34-35 0.19253118216499132 1102 36-37 0.2651042128140091 1102 38-39 0.05180240923694157 1102 40-41 0.31203525852731673 1102 42-43 0.273438347562184 1102 44-45 -0.05532959123885206 1102 46-47 0.20258854911001123 1102 48-49 0.4408475442961759 1102 50-51 0.17850999819820146 1102 52-53 0.1269689033799679 1102 54-55 0.3412240761613745 1102 56-57 0.4611609905898746 1102 58-59 0.35205344028448593 1102 60-61 0.22740367310083087 1102 62-63 0.41594384118190675 1102 64-65 0.34531110954674205 1102 66-67 0.19957782119945477 1102 68-69 0.2788893339180518 1102 70-71 0.21957623664109605 1102 72-73 0.15528738161354383 1102 74-75 0.12787969547417788 1102 76-77 0.28702290563853694 1102 78-79 0.3243025704026117 1102 80-81 0.22620295995991313 1102 82-83 0.20643125063299195 1102 84-85 -0.0033253710007237203 1102 86-87 -0.07550984888651513 1102 88-89 0.09564802048629417 1102 90-91 -0.07532167633042164 1102 92-93 -0.2057974851679525 1102 94-95 0.18758923140195805 1102 96-97 0.0402109442005667 1102 98-99 -0.054273688589670854 1102 100-101 0.1692054903244653 1103 1 0.18990678224603386 1103 2 0.2930860505476076 1103 3 0.266690126609344 1103 4 0.28954737841987566 1103 5 0.34123433838786354 1103 6 0.2939879529954368 1103 7 0.31786524083393886 1103 8 0.3579189930631159 1103 9 0.44950167046979317 1103 10-11 0.31901990851032735 1103 12-13 0.3688348276970359 1103 14-15 0.43854247601374396 1103 16-17 0.39904302647914847 1103 18-19 0.4081464087698876 1103 20-21 0.44116509887719957 1103 22-23 0.6410956404876345 1103 24-25 0.3276593971709758 1103 26-27 0.47209656877132744 1103 28-29 0.4657714729327225 1103 30-31 0.594652546617084 1103 32-33 0.7587031499312147 1103 34-35 0.6555764557645176 1103 36-37 0.7817605176197446 1103 38-39 0.8201730607350797 1103 40-41 0.6832203151773157 1103 42-43 0.6021011107457142 1103 44-45 0.6016154847120347 1103 46-47 0.8504909682179047 1103 48-49 0.8407705262850911 1103 50-51 0.6195541026086389 1103 52-53 0.8280647861641199 1103 54-55 0.9031586201312294 1103 56-57 0.9407372987361526 1103 58-59 1.0766815732008652 1103 60-61 0.9768074794608452 1103 62-63 1.022530273020969 1103 64-65 1.0478731183249437 1103 66-67 0.9424350966177144 1103 68-69 0.9832376037965815 1103 70-71 0.956694278342173 1103 72-73 0.8782870011631303 1103 74-75 1.08721325769638 1103 76-77 0.8957250129626715 1103 78-79 0.839631938390955 1103 80-81 1.0872086481575955 1103 82-83 0.9100696557106147 1103 84-85 0.9779072883476836 1103 86-87 1.0407966271219102 1103 88-89 1.1396781966997906 1103 90-91 1.0196378196191702 1103 92-93 1.1728620302681279 1103 94-95 1.4186479042795561 1103 96-97 1.4357309085681393 1103 98-99 1.356246229381508 1103 100-101 1.3688955624244592 1104 1 0.34034253750039056 1104 2 0.3710202125769513 1104 3 0.36473505883979485 1104 4 0.358966820364067 1104 5 0.3111880855874034 1104 6 0.3295472571010265 1104 7 0.25011293640641696 1104 8 0.29931541372707926 1104 9 0.37441724596894943 1104 10-11 0.4807084313772165 1104 12-13 0.43110241394471416 1104 14-15 0.7759431868877584 1104 16-17 0.6013834247525551 1104 18-19 0.5335410193988324 1104 20-21 0.6879839869703943 1104 22-23 0.6713274957899529 1104 24-25 0.513441752778796 1104 26-27 0.6890355071907166 1104 28-29 0.5203402509784141 1104 30-31 0.5688574944841349 1104 32-33 0.6263677448578662 1104 34-35 0.4662508169962578 1104 36-37 0.6878325116804689 1104 38-39 0.6119619567779679 1104 40-41 0.5251596642217038 1104 42-43 0.3290576567652792 1104 44-45 0.2967173854130536 1104 46-47 0.19483863291137737 1104 48-49 0.24991810162656947 1104 50-51 0.04940415878214566 1104 52-53 -0.07439124411043707 1104 54-55 0.018624728785894717 1104 56-57 0.05341731673794925 1104 58-59 0.12303456616439234 1104 60-61 0.1466978261597518 1104 62-63 0.308478849630454 1104 64-65 0.2105824549520321 1104 66-67 0.09404154375878448 1104 68-69 0.10465859626079421 1104 70-71 0.04103870731061576 1104 72-73 -0.012246942742212497 1104 74-75 0.08068883700613583 1104 76-77 0.03793061125972841 1104 78-79 0.19934190573805566 1104 80-81 0.4096539857570498 1104 82-83 0.04359792642089744 1104 84-85 0.257868952397299 1104 86-87 0.19517027616564775 1104 88-89 -0.035373706300728 1104 90-91 -0.08328451641004975 1104 92-93 -0.06323297521781868 1104 94-95 0.24623180318039672 1104 96-97 0.13346861117051745 1104 98-99 0.2587663171567094 1104 100-101 0.5871678002321836 1105 1 0.21373210649908714 1105 2 0.3012559702222788 1105 3 0.13895145079911586 1105 4 0.19442010436036838 1105 5 0.3071349987591745 1105 6 0.16442828102454143 1105 7 0.049120512522073057 1105 8 0.11420109675914603 1105 9 0.19179116306382582 1105 10-11 0.3189470520109907 1105 12-13 0.2629056408838153 1105 14-15 0.2660932236879603 1105 16-17 0.29546314952753505 1105 18-19 0.34604186344023447 1105 20-21 0.2344370717729234 1105 22-23 0.27686643310806147 1105 24-25 0.062381805582027994 1105 26-27 0.13262399986781048 1105 28-29 0.013981243030919188 1105 30-31 -0.04553501515481173 1105 32-33 0.14947169024500795 1105 34-35 -0.07106720767676222 1105 36-37 -0.10019155362807908 1105 38-39 0.07281067417265064 1105 40-41 0.07825366680109624 1105 42-43 0.11180393116651999 1105 44-45 0.07632720195221765 1105 46-47 0.2619711541643355 1105 48-49 -0.0044177390724371435 1105 50-51 -0.15173977053445498 1105 52-53 -0.018543556446275034 1105 54-55 -0.04956875251352244 1105 56-57 0.17477485605339638 1105 58-59 0.06475350712876349 1105 60-61 0.045632308289704326 1105 62-63 -0.05906151242954394 1105 64-65 0.05033275374269408 1105 66-67 0.045877587027717937 1105 68-69 0.03610977352974487 1105 70-71 -0.17477381758860489 1105 72-73 -0.4455339100676774 1105 74-75 -0.06107677944794787 1105 76-77 -0.041634217872530854 1105 78-79 0.11760071584224363 1105 80-81 0.10460011903456845 1105 82-83 0.21968479853849843 1105 84-85 0.06687673108288905 1105 86-87 0.06566900335120707 1105 88-89 0.14040551272464086 1105 90-91 -0.034576574993277376 1105 92-93 0.031307680568588125 1105 94-95 0.09817048188421751 1105 96-97 -0.052297727783006565 1105 98-99 -0.2142218257496289 1105 100-101 -0.007651389217972593 1106 1 -0.015797548870835953 1106 2 0.18976973601017022 1106 3 0.1902431656739374 1106 4 0.10504944144668826 1106 5 0.29710770350214943 1106 6 0.1514386553306295 1106 7 0.32831927769061764 1106 8 0.2801530968153756 1106 9 0.4215202398480926 1106 10-11 0.3688911349492088 1106 12-13 0.5006313406816716 1106 14-15 0.5707924531482149 1106 16-17 0.38222596015712895 1106 18-19 0.3908045036391954 1106 20-21 0.49961923488909576 1106 22-23 0.3394671870440007 1106 24-25 0.48896441656180656 1106 26-27 0.47379802096782697 1106 28-29 0.5039688824172615 1106 30-31 0.5891925989129163 1106 32-33 0.5203177729319819 1106 34-35 0.6345538406825 1106 36-37 0.5798095532925487 1106 38-39 0.5169401733437766 1106 40-41 0.5463352591503394 1106 42-43 0.5732738690471919 1106 44-45 0.7363883637093451 1106 46-47 0.7275718386981112 1106 48-49 0.4672784762253883 1106 50-51 0.6642649839531813 1106 52-53 0.9159517799917083 1106 54-55 0.7917910731201161 1106 56-57 0.8411072502612171 1106 58-59 0.8402502377596335 1106 60-61 0.8123940295688499 1106 62-63 0.829980676180945 1106 64-65 0.9230619192563125 1106 66-67 0.861638555425138 1106 68-69 0.8543353973366266 1106 70-71 0.8053240131105248 1106 72-73 0.9321039213166848 1106 74-75 0.9021075676401971 1106 76-77 0.9606015860900676 1106 78-79 0.9220658228050382 1106 80-81 0.8595863132745372 1106 82-83 0.8837084725837556 1106 84-85 0.9758878556351824 1106 86-87 1.1674964765940352 1106 88-89 1.1954743746081817 1106 90-91 1.173922906247494 1106 92-93 0.904068911640028 1106 94-95 0.7133837881243501 1106 96-97 0.8507540348123896 1106 98-99 0.9465847504130984 1106 100-101 0.8142029136724069 1107 1 0.3293467766656448 1107 2 0.37628764801828396 1107 3 0.2616039037013387 1107 4 0.25800977087760657 1107 5 0.34763161677116017 1107 6 0.21362193320018008 1107 7 0.06232456071550985 1107 8 0.24717010304806308 1107 9 0.2626544143156053 1107 10-11 0.28172920465002704 1107 12-13 0.33427590962035936 1107 14-15 0.3970950539606122 1107 16-17 0.3326317635041107 1107 18-19 0.3062042817840762 1107 20-21 0.4673425065339316 1107 22-23 0.3890174914962685 1107 24-25 0.3004075123186212 1107 26-27 0.09896261672066231 1107 28-29 0.29784711747196724 1107 30-31 0.32553613917802693 1107 32-33 0.3784302486915152 1107 34-35 0.16880172787156056 1107 36-37 0.34756793869799196 1107 38-39 0.1835110752087914 1107 40-41 0.06561191568189884 1107 42-43 0.1900111622550682 1107 44-45 0.3506517328746739 1107 46-47 0.2025732352681402 1107 48-49 0.02545958342609822 1107 50-51 -0.10580372729199183 1107 52-53 0.014577555088074234 1107 54-55 0.21685511583122263 1107 56-57 0.3669677369060196 1107 58-59 0.2946781627757886 1107 60-61 0.37272984480662075 1107 62-63 0.43062150231197194 1107 64-65 0.537955740032487 1107 66-67 0.5133300012342303 1107 68-69 0.30664851631641454 1107 70-71 0.32287291301236465 1107 72-73 0.4647477480781568 1107 74-75 0.4287301824981036 1107 76-77 0.40392877451529685 1107 78-79 0.34412524486446117 1107 80-81 0.40488705465370955 1107 82-83 0.32363202035044125 1107 84-85 0.47423591280676547 1107 86-87 0.31594388974733434 1107 88-89 0.5205200623989654 1107 90-91 0.5924909032468619 1107 92-93 0.25873784474033457 1107 94-95 0.13612553439550723 1107 96-97 0.17826566624558637 1107 98-99 0.1927840848477267 1107 100-101 0.49862198044441186 1108 1 0.08089588115592505 1108 2 0.2655469329994915 1108 3 0.12950368069202867 1108 4 0.3131679222924433 1108 5 0.317513784514297 1108 6 0.17273946451022226 1108 7 0.1926345359452668 1108 8 0.20058507785341817 1108 9 0.21372354720291753 1108 10-11 0.12260935836440723 1108 12-13 0.2487182705867852 1108 14-15 0.18735826668372368 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-1.9755879708800883 2316 76-77 -2.0882348263202246 2316 78-79 -2.0957536620247588 2316 80-81 -2.103037902270639 2316 82-83 -1.736258533809366 2316 84-85 -1.8494359614412126 2316 86-87 -2.229492979158305 2316 88-89 -2.1806874996636587 2316 90-91 -2.105359164615333 2316 92-93 -2.5996587785137812 2316 94-95 -2.0760939925905433 2316 96-97 -1.9806887746372936 2316 98-99 -2.0540393868089772 2316 100-101 -2.3305602078948375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1598.0 3 558.0 4 529.0 5 576.0 6 650.0 7 835.0 8 1074.0 9 1414.0 10 1862.0 11 2778.0 12 4347.0 13 5816.0 14 7343.0 15 9897.0 16 13605.0 17 19564.0 18 16329.0 19 7541.0 20 6557.0 21 6678.0 22 6978.0 23 7355.0 24 8244.0 25 9332.0 26 10734.0 27 12509.0 28 14812.0 29 17776.0 30 21613.0 31 26773.0 32 33993.0 33 45365.0 34 62852.0 35 92863.0 36 131612.0 37 184084.0 38 164358.0 39 28900.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.712199874477115 19.44917485378403 15.129752461188465 18.70887281055039 2 15.184132771227512 22.11876978047962 40.934932074388755 21.762165373904114 3 19.04957270484854 29.579010445666263 30.87721567466353 20.49420117482167 4 11.65009100528245 17.837439603518128 38.697507738733485 31.814961652465932 5 11.835806666525132 40.16282650475003 32.292440689035345 15.708926139689492 6 27.18383008488882 38.18379571694562 18.464783977260552 16.16759022090501 7 25.526141868826812 37.56075920457738 20.389222706611612 16.523876219984196 8 27.07412809945342 32.534847212071405 19.9311193261137 20.45990536236147 9 29.163491412157803 16.26970089607872 20.62668911748475 33.94011857427872 10-11 24.036637536799745 28.529768756625973 26.031934041923154 21.401659664651124 12-13 27.177487457908754 26.964119031483214 27.177436920621588 18.68095658998644 14-15 24.00203937674706 26.057080315870962 28.851723768541852 21.089156538840122 16-17 19.495716709632628 30.394687244515907 28.58122385788187 21.528372187969598 18-19 19.427175125713454 27.160017866670977 34.313103303313014 19.099703704302552 20-21 21.918231811944082 25.50455850877849 33.80567272109929 18.771536958178135 22-23 20.87626378856415 26.33914474398856 33.67539599255684 19.109195474890452 24-25 20.082035333089824 26.423834401591193 34.384969922567095 19.10916034275189 26-27 20.08524727975597 26.39048447573984 34.29174636180938 19.23252188269481 28-29 19.481310757845662 26.674746662328598 34.580731079624165 19.263211500201578 30-31 19.685836948032197 26.83329140181609 34.35055152575151 19.130320124400203 32-33 19.3249210750864 27.08444372144671 33.95951223923456 19.631122964232326 34-35 19.83749768871456 26.809294449176473 33.926992681687246 19.426215180421718 36-37 20.229734194596595 26.608882004710477 33.70596811790133 19.455415682791607 38-39 20.134524526425977 26.674534307088553 33.82492613983114 19.366015026654328 40-41 19.79935516399265 27.008140776733363 33.69743025475165 19.495073804522338 42-43 19.75393777362841 27.539963767127507 32.901388185820274 19.804710273423805 44-45 19.767183973867052 27.717807819170996 32.45177746780997 20.063230739151983 46-47 20.250750473118867 27.85828965887953 31.494762601936525 20.396197266065073 48-49 20.435453428499834 28.239756533266515 30.758944088333486 20.565845949900172 50-51 20.686606554660568 28.765706652871355 30.28792330667227 20.25976348579581 52-53 20.59291285198549 29.060924091675616 30.11613538148159 20.230027674857308 54-55 20.00901288681148 28.966428007040562 30.145832954428336 20.878726151719622 56-57 20.604210040259453 29.505090150554885 29.26213909087097 20.628560718314688 58-59 20.688420037971063 28.68967496243815 29.76343360267394 20.858471396916848 60-61 20.59950903148655 28.66790309762837 30.22679633193271 20.505791538952376 62-63 21.084946339230637 28.682834363707 30.047858444491933 20.18436085257043 64-65 20.72352666581792 28.8931730413643 30.174276089377123 20.20902420344066 66-67 20.491148931182156 29.18235485520383 29.86081216817334 20.465684045440675 68-69 20.49429275482265 29.395230581193655 29.750686026483073 20.359790637500623 70-71 20.39918804073137 29.60397025241584 29.38253600327856 20.614305703574228 72-73 20.426585256418033 29.533952600964476 29.114421013060404 20.925041129557094 74-75 20.305376991726384 29.990116529952065 29.11732932065797 20.58717715766358 76-77 20.51035986252545 29.721922804230154 29.20333589544172 20.56438143780267 78-79 20.24488603835288 29.6610533475018 29.249036023977244 20.845024590168077 80-81 20.073752507570095 30.228773283315867 28.951801583920012 20.745672625194022 82-83 20.183148702191318 30.04533233605563 28.790632327969597 20.980886633783456 84-85 20.218126400934597 29.686018849581313 28.72236785990755 21.37348688957654 86-87 20.499714521026917 29.69223850883974 28.680076599313843 21.1279703708195 88-89 20.559274847537097 29.394847334993962 28.70182955481338 21.34404826265556 90-91 20.003395724721937 29.51228398364999 29.26215247511444 21.222167816513632 92-93 20.08842616783891 30.028360249966397 28.864690478091553 21.01852310410314 94-95 19.907486471601732 29.87105705927232 28.707387440189574 21.514069028936373 96-97 20.004446532967503 29.70374974103998 28.434214050033603 21.85758967595891 98-99 20.012461518870158 29.706320506626945 28.429040089100365 21.852177885402536 100-101 20.19868101922069 29.687529218168557 28.715451805752306 21.398337956858445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2091.0 1 1444.0 2 984.5 3 1205.0 4 1138.5 5 1239.5 6 2079.5 7 4041.0 8 6428.0 9 7252.5 10 5910.0 11 4589.5 12 4466.0 13 4541.0 14 4470.5 15 4319.0 16 4119.5 17 4116.0 18 4226.5 19 4280.5 20 4545.5 21 4925.0 22 5380.0 23 6047.5 24 6770.0 25 7845.5 26 9276.5 27 10531.5 28 11405.0 29 12634.0 30 14457.0 31 16273.0 32 18516.0 33 20411.0 34 22327.0 35 23790.0 36 25435.5 37 27754.0 38 28028.5 39 27414.5 40 27681.5 41 28387.5 42 28610.5 43 29581.0 44 31369.5 45 32588.5 46 32301.0 47 33297.0 48 42848.0 49 42428.5 50 32087.0 51 28043.0 52 25170.5 53 24056.0 54 22935.5 55 21290.0 56 19924.0 57 18305.0 58 16826.5 59 15692.5 60 14220.0 61 12222.5 62 9980.0 63 7113.5 64 4622.0 65 3336.0 66 2483.5 67 1883.0 68 1349.0 69 873.0 70 620.0 71 480.0 72 357.0 73 317.5 74 238.5 75 169.0 76 135.0 77 77.5 78 44.5 79 27.5 80 16.0 81 11.5 82 10.0 83 9.5 84 6.5 85 3.5 86 3.5 87 2.5 88 1.5 89 2.5 90 3.0 91 1.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025057870545786693 2 0.009800860657021408 3 0.01131645766583915 4 0.02081419892109701 5 0.05638020872802005 6 0.041931517243957564 7 0.014852850686413887 8 0.046680387871586494 9 0.028998422768712822 10-11 0.0234412337363811 12-13 0.03440405210016277 14-15 0.019298601912279265 16-17 0.016772606897583028 18-19 0.01586324869229238 20-21 0.013236213877008293 22-23 0.007628504944382641 24-25 0.001364037307935969 26-27 0.0 28-29 0.0022228756129326903 30-31 0.0 32-33 2.525995014696239E-4 34-35 0.0 36-37 0.0 38-39 1.0103980058784955E-4 40-41 0.0 42-43 0.0 44-45 5.0519900293924773E-5 46-47 0.0 48-49 5.051990029392478E-4 50-51 1.0103980058784955E-4 52-53 2.020796011756991E-4 54-55 0.0015155970088177434 56-57 3.5363930205747343E-4 58-59 8.083184047027964E-4 60-61 0.003283793519105111 62-63 0.001970276111463066 64-65 0.0015155970088177434 66-67 0.011215417865251301 68-69 0.013488813378477914 70-71 0.02531047004725631 72-73 0.015459089489940984 74-75 0.017985084504637223 76-77 0.029251022270182447 78-79 0.018944962610221792 80-81 0.04592258936717762 82-83 0.035262890405159494 84-85 0.019298601912279265 86-87 0.014549731284650336 88-89 0.012528935272893347 90-91 0.023188634234911475 92-93 0.02990778097400347 94-95 0.012528935272893347 96-97 0.017580925302285822 98-99 0.026573467554604435 100-101 0.03884980332602816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 989709.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.57356400234843 #Duplication Level Percentage of deduplicated Percentage of total 1 75.81931947342369 38.34453206003696 2 13.24253411262413 13.394442929961578 3 4.538902016647328 6.88645354917906 4 2.0663432227227942 4.180093649007606 5 1.143304627221811 2.8910494869491687 6 0.6862320036454885 2.082311889409495 7 0.46683116983323775 1.652652123209671 8 0.3178801567710684 1.2861065962830547 9 0.22962269245389014 1.0451554139887544 >10 1.3173671905350972 12.290545141311867 >50 0.10513228885410256 3.6878218531675366 >100 0.059496040581702596 5.292966533734524 >500 0.004020002677537633 1.3477145638519659 >1k 0.0024120016065225795 2.2254876260681162 >5k 2.0100013387688165E-4 0.7450143338717045 >10k+ 4.020002677537633E-4 2.647652249968947 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15351 1.5510619788240787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10695 1.080620667287051 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7329 0.7405206985083495 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3059 0.3090807499982318 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2879 0.29089358589241887 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2510 0.2536098994755024 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2277 0.23006762593853347 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1985 0.20056400416688136 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1841 0.18601427288223105 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1813 0.18318515846577124 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1237 0.1249862333271699 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1108 0.11195209905133731 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1097 0.11084066124487098 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1070 0.10811258662899903 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.102757477197843 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.051990029392478E-5 0.0 12-13 0.0 0.0 0.0 2.0207960117569912E-4 1.5155970088177433E-4 14-15 0.0 0.0 0.0 2.525995014696239E-4 2.0207960117569912E-4 16-17 0.0 5.051990029392478E-5 0.0 4.0415920235139824E-4 2.0207960117569912E-4 18-19 0.0 1.0103980058784956E-4 0.0 4.5467910264532306E-4 2.0207960117569912E-4 20-21 0.0 1.0103980058784956E-4 0.0 5.051990029392478E-4 2.0207960117569912E-4 22-23 0.0 1.0103980058784956E-4 0.0 0.001364037307935969 2.0207960117569912E-4 24-25 0.0 1.0103980058784956E-4 0.0 0.0023744353138144647 2.0207960117569912E-4 26-27 0.0 1.0103980058784956E-4 0.0 0.0036374328211625844 2.0207960117569912E-4 28-29 0.0 1.0103980058784956E-4 0.0 0.0055571890323317255 2.0207960117569912E-4 30-31 0.0 1.0103980058784956E-4 0.0 0.009800860657021408 2.0207960117569912E-4 32-33 0.0 1.0103980058784956E-4 0.0 0.014044532281711089 2.0207960117569912E-4 34-35 0.0 1.0103980058784956E-4 0.0 0.018237684006106848 2.0207960117569912E-4 36-37 0.0 1.0103980058784956E-4 0.0 0.024653711343435292 2.0207960117569912E-4 38-39 0.0 1.0103980058784956E-4 0.0 0.033646253595753906 2.525995014696239E-4 40-41 0.0 1.0103980058784956E-4 0.0 0.0515808182000972 3.0311940176354867E-4 42-43 0.0 1.0103980058784956E-4 0.0 0.0670904275903321 3.0311940176354867E-4 44-45 0.0 1.0103980058784956E-4 0.0 0.08451979319173615 3.0311940176354867E-4 46-47 0.0 1.0103980058784956E-4 0.0 0.11867124579042931 3.0311940176354867E-4 48-49 0.0 1.0103980058784956E-4 0.0 0.14175884022475294 3.0311940176354867E-4 50-51 0.0 1.0103980058784956E-4 0.0 0.1718687007999321 3.0311940176354867E-4 52-53 0.0 1.0103980058784956E-4 0.0 0.20642431260097666 3.0311940176354867E-4 54-55 0.0 1.0103980058784956E-4 0.0 0.24739595173934964 3.0311940176354867E-4 56-57 0.0 1.5155970088177433E-4 0.0 0.35732725477893 3.0311940176354867E-4 58-59 0.0 2.0207960117569912E-4 0.0 0.4443225230850685 3.0311940176354867E-4 60-61 0.0 2.0207960117569912E-4 0.0 0.5113624307751066 3.0311940176354867E-4 62-63 0.0 2.0207960117569912E-4 0.0 0.590325034934511 3.0311940176354867E-4 64-65 0.0 2.0207960117569912E-4 0.0 0.6585774202316035 3.0311940176354867E-4 66-67 0.0 2.0207960117569912E-4 0.0 0.7799262207376108 3.0311940176354867E-4 68-69 0.0 2.0207960117569912E-4 0.0 0.9393165061649434 3.0311940176354867E-4 70-71 0.0 2.0207960117569912E-4 0.0 1.0173697521190572 3.0311940176354867E-4 72-73 0.0 2.0207960117569912E-4 0.0 1.1102253288592911 3.0311940176354867E-4 74-75 0.0 2.0207960117569912E-4 0.0 1.2866913405859703 3.0311940176354867E-4 76-77 0.0 2.0207960117569912E-4 0.0 1.4659864667291092 3.0311940176354867E-4 78-79 0.0 4.0415920235139824E-4 0.0 1.635480732215227 3.0311940176354867E-4 80-81 0.0 4.0415920235139824E-4 0.0 1.7886065500061128 3.0311940176354867E-4 82-83 0.0 4.0415920235139824E-4 0.0 2.028929715704313 3.0311940176354867E-4 84-85 0.0 4.0415920235139824E-4 0.0 2.4390502662903946 3.0311940176354867E-4 86-87 0.0 4.0415920235139824E-4 0.0 2.789658374330233 3.5363930205747343E-4 88-89 0.0 4.0415920235139824E-4 0.0 3.2190270069282994 4.0415920235139824E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGA 70 2.0481588E-4 33.942566 6 GTATCAA 15525 0.0 30.392637 1 CACGGTA 35 0.0083916485 27.143759 66-67 GGTATCA 10560 0.0 26.548431 1 GTACATG 10990 0.0 25.466448 1 TACATGG 11025 0.0 25.172647 2 TATCAAC 18805 0.0 25.169851 2 ATCAACG 18935 0.0 24.971949 3 ATGGGAG 2975 0.0 24.919096 5 TCAACGC 19200 0.0 24.602533 4 CAACGCA 19445 0.0 24.26811 5 ACATGGG 10955 0.0 24.075497 3 AACGCAG 19800 0.0 23.855795 6 GTACGTG 100 0.0016500046 23.758595 1 GAGTACT 9285 0.0 23.7416 12-13 AGTACTT 9455 0.0 23.490595 12-13 CATGGGG 2110 0.0 23.197939 4 CTTTGCG 165 2.4195597E-6 23.040968 2 GTATAGG 310 0.0 22.99219 1 CATGGGA 8485 0.0 22.570847 4 >>END_MODULE