FastQCFastQC Report
Thu 26 May 2016
SRR936821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989709
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186311.8824725247522254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143071.4455764270103637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102701.037678752037215No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.3431311627963371No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31150.3147389788311514No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30130.30443291917119075No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29840.3015027649541431No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27930.2822041630418638No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25490.25755045169842855No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.16479591475878264No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16250.16418967595525552No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.1476191486588482No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.14479003424238843No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13590.13731308899888756No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT12040.12165191990777087No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG11390.11508433286956064No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10840.10952714383722893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATC459.630374E-442.2169232
GGTATCA112200.031.3288041
GTATCAA167300.031.2889211
TAATACC2550.026.0725234
TATCAAC206150.025.342442
ATCAACG207950.025.0520293
TCAACGC209900.024.8192944
CAACGCA211450.024.6048745
GAGTACT120600.024.45341312-13
AACGCAG214750.024.2710256
AGTACTT121600.023.99846812-13
GTACATG104150.023.3971521
TACATGG105700.022.6910952
GTACTTT130550.022.39073414-15
ACGCAGA233000.022.3087947
CGCAGAG233750.022.1762338
GTGTACG1302.9355695E-421.9291889
ACATGGG104200.021.6937393
GCAGAGT237000.021.69179
GGATATG3950.021.6461331
Produced by FastQC (version 0.11.5)