##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936821_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 989709 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19432075488856 33.0 31.0 34.0 31.0 34.0 2 32.2730145931784 33.0 31.0 34.0 30.0 34.0 3 32.33680506088153 34.0 31.0 34.0 30.0 34.0 4 36.03306224354836 37.0 35.0 37.0 35.0 37.0 5 35.971294592652995 37.0 35.0 37.0 35.0 37.0 6 36.05759167593707 37.0 35.0 37.0 35.0 37.0 7 36.00385668918844 37.0 35.0 37.0 35.0 37.0 8 35.98262923748294 37.0 35.0 37.0 35.0 37.0 9 37.62669633195212 39.0 37.0 39.0 35.0 39.0 10-11 37.56005300547939 39.0 37.0 39.0 35.0 39.0 12-13 37.449618524232875 39.0 37.0 39.0 35.0 39.0 14-15 38.51824728278716 40.0 38.0 41.0 34.0 41.0 16-17 38.472367130136234 40.0 38.0 41.0 34.0 41.0 18-19 38.75014473951434 40.0 38.0 41.0 35.0 41.0 20-21 38.83700309889069 40.0 38.0 41.0 35.0 41.0 22-23 38.7540559901951 40.0 38.0 41.0 35.0 41.0 24-25 38.626353807028124 40.0 38.0 41.0 34.0 41.0 26-27 38.39789827110798 40.0 38.0 41.0 34.0 41.0 28-29 38.24855942504312 40.0 38.0 41.0 34.0 41.0 30-31 38.03268283909715 40.0 37.0 41.0 34.0 41.0 32-33 37.65932612515396 40.0 35.5 41.0 33.0 41.0 34-35 37.41138506369044 40.0 35.0 41.0 33.0 41.0 36-37 37.14564432575636 39.5 35.0 41.0 33.0 41.0 38-39 36.9035064852396 39.0 35.0 41.0 32.5 41.0 40-41 36.608725898218566 39.0 35.0 41.0 31.5 41.0 42-43 36.19252123604009 39.0 35.0 41.0 30.5 41.0 44-45 35.638780186903425 39.0 35.0 41.0 29.0 41.0 46-47 34.87741699832981 39.0 35.0 40.5 24.0 41.0 48-49 34.18760918613451 39.0 34.5 40.0 18.5 41.0 50-51 33.79817148272876 38.0 34.0 40.0 11.0 41.0 52-53 33.52898528759464 38.0 34.0 40.0 7.5 41.0 54-55 33.24644668281283 38.0 33.0 40.0 6.0 41.0 56-57 32.975957579450125 38.0 33.0 40.0 2.0 41.0 58-59 32.6356994833835 37.0 33.0 40.0 2.0 41.0 60-61 32.29751169283092 37.0 32.5 40.0 2.0 41.0 62-63 31.919210596245968 36.0 32.0 39.0 2.0 41.0 64-65 31.521127422302918 36.0 31.0 39.0 2.0 40.0 66-67 31.143543708302136 35.0 31.0 39.0 2.0 40.0 68-69 30.85769453445407 35.0 31.0 38.0 2.0 40.0 70-71 30.409971516880212 35.0 30.0 37.5 2.0 40.0 72-73 29.92914735543478 35.0 29.5 37.0 2.0 39.0 74-75 29.427850509594236 34.0 29.0 36.5 2.0 39.0 76-77 28.03534220664862 32.5 27.0 35.0 2.0 37.0 78-79 28.72537836879325 34.0 29.0 35.0 2.0 37.5 80-81 28.502566916133933 34.0 29.0 35.0 2.0 37.0 82-83 28.181247720289498 34.0 28.5 35.0 2.0 37.0 84-85 27.839607399750836 34.0 27.5 35.0 2.0 36.0 86-87 27.477626756955832 34.0 27.0 35.0 2.0 36.0 88-89 27.06578701416275 33.5 26.0 35.0 2.0 35.5 90-91 26.60477322121957 33.0 25.0 35.0 2.0 35.0 92-93 26.15306468871153 33.0 24.5 35.0 2.0 35.0 94-95 25.609057813963496 32.5 21.5 35.0 2.0 35.0 96-97 24.994673181713008 32.0 18.5 34.0 2.0 35.0 98-99 24.251079862868785 32.0 2.0 34.0 2.0 35.0 100-101 21.020380232977573 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12131235287786524 1101 2 0.14786358719062065 1101 3 0.13303521182289302 1101 4 0.18162702944806597 1101 5 0.23456283207365658 1101 6 0.27245907435433736 1101 7 0.23054312756856632 1101 8 0.16248741191354554 1101 9 0.2041268503236111 1101 10-11 0.2904802869508316 1101 12-13 0.342972533920765 1101 14-15 0.40951707330957277 1101 16-17 0.2380300996614224 1101 18-19 0.28055156539321047 1101 20-21 0.3056207007904206 1101 22-23 0.307251725653245 1101 24-25 0.34077964549781825 1101 26-27 0.24953378616585553 1101 28-29 0.2305008057364475 1101 30-31 0.23112446207701254 1101 32-33 0.33144472674586467 1101 34-35 0.3321451524414698 1101 36-37 0.39013135799341114 1101 38-39 0.20808750424358635 1101 40-41 0.47697249090248306 1101 42-43 0.6695901500999852 1101 44-45 0.7604352435983586 1101 46-47 0.7089162089767029 1101 48-49 0.7997884070428611 1101 50-51 0.6439114745853445 1101 52-53 0.719318631924267 1101 54-55 0.7877464576290691 1101 56-57 0.7311337085455492 1101 58-59 0.7790951266506099 1101 60-61 0.9106018055157179 1101 62-63 0.7864519280375823 1101 64-65 0.7863241987023564 1101 66-67 0.6194266888969544 1101 68-69 0.5554355470975416 1101 70-71 0.6935257841714737 1101 72-73 0.9249318775778903 1101 74-75 0.8546347264757088 1101 76-77 0.6847357495545374 1101 78-79 0.7431869206603992 1101 80-81 0.9338790537675159 1101 82-83 0.9366962286696499 1101 84-85 0.8534516944390056 1101 86-87 0.5002209221392278 1101 88-89 0.5811744219997159 1101 90-91 0.7579257294344934 1101 92-93 0.48307321039285966 1101 94-95 0.34594303690372286 1101 96-97 0.4970133230531104 1101 98-99 0.4316083424914048 1101 100-101 1.0140283654519244 1102 1 0.3045996223483698 1102 2 0.3041444236501931 1102 3 0.27389593788849 1102 4 0.2709360538057126 1102 5 0.26871115505700516 1102 6 0.297145941861217 1102 7 0.23443886482489518 1102 8 0.26633597906634066 1102 9 0.28882683342025217 1102 10-11 0.3057313637655312 1102 12-13 0.3966265354588856 1102 14-15 0.579316613644437 1102 16-17 0.4090850019449732 1102 18-19 0.43090604916377373 1102 20-21 0.35378686345381993 1102 22-23 0.3063370858407879 1102 24-25 0.46287498928645476 1102 26-27 0.3628004441595962 1102 28-29 0.5170335199289937 1102 30-31 0.5633590771450727 1102 32-33 0.713888952302149 1102 34-35 0.6887542326421467 1102 36-37 0.5665541826595728 1102 38-39 0.6937085875983868 1102 40-41 0.6761161720998388 1102 42-43 0.8513193901657203 1102 44-45 0.6151973443229082 1102 46-47 0.4118051786949124 1102 48-49 0.39342637251576207 1102 50-51 0.5064628373411395 1102 52-53 0.42342490891100937 1102 54-55 0.370696367935075 1102 56-57 0.3639259574266944 1102 58-59 0.4717628747781859 1102 60-61 0.349913883097706 1102 62-63 0.4787108096016759 1102 64-65 0.33676877471569355 1102 66-67 0.04258809472534253 1102 68-69 0.18926235228324373 1102 70-71 0.3425182643896747 1102 72-73 0.3757386810467054 1102 74-75 0.515921080863901 1102 76-77 0.34548137348756924 1102 78-79 0.34627129673621937 1102 80-81 0.5198764359595174 1102 82-83 0.4936335526114455 1102 84-85 0.5541312802270859 1102 86-87 0.4757141506186393 1102 88-89 0.6829490090843109 1102 90-91 0.9931855145760018 1102 92-93 0.9541746632851442 1102 94-95 0.8183896784897016 1102 96-97 0.8961418708776634 1102 98-99 1.057633495749993 1102 100-101 1.4559852433043652 1103 1 0.23309350266423223 1103 2 0.23146911488220212 1103 3 0.23019053642050835 1103 4 0.1212334546798246 1103 5 0.10480198063440582 1103 6 0.07148316939840527 1103 7 0.12614968539148919 1103 8 0.05289401631335977 1103 9 0.08705820294895972 1103 10-11 0.09405710355600405 1103 12-13 0.22584141570980165 1103 14-15 0.410772439639409 1103 16-17 0.2545478987501113 1103 18-19 0.21307498770710964 1103 20-21 0.12992891064823198 1103 22-23 0.16585344984832773 1103 24-25 0.04941354004874654 1103 26-27 0.10602425107637004 1103 28-29 0.09458508951893663 1103 30-31 0.10912911806669001 1103 32-33 0.3124905650407399 1103 34-35 0.3749104771325733 1103 36-37 0.42778257353990057 1103 38-39 0.3089201087504989 1103 40-41 0.31960165466872326 1103 42-43 0.4374867697902758 1103 44-45 0.5454960442959944 1103 46-47 0.4399121130996946 1103 48-49 0.5011170384895749 1103 50-51 0.642538816073035 1103 52-53 0.665159391637637 1103 54-55 0.6604279184097095 1103 56-57 0.5627994530236791 1103 58-59 0.44065873343622286 1103 60-61 0.7290976777658287 1103 62-63 0.7334919578195915 1103 64-65 0.6723353210610519 1103 66-67 0.7536174450830551 1103 68-69 0.5632265528270075 1103 70-71 0.47443248673974736 1103 72-73 0.6232402353258486 1103 74-75 0.8767783006064924 1103 76-77 0.6909025135397187 1103 78-79 0.7523603592507158 1103 80-81 1.0679338190174086 1103 82-83 0.9323207202972839 1103 84-85 0.9259658863681146 1103 86-87 0.6872445820122941 1103 88-89 0.7612893173419408 1103 90-91 0.8712418769132633 1103 92-93 0.8742290398568251 1103 94-95 0.6914909061382417 1103 96-97 0.5693859994142301 1103 98-99 0.43727661470106227 1103 100-101 0.7817528716135875 1104 1 0.22692345135253333 1104 2 0.2509442375769524 1104 3 0.26522506177085603 1104 4 0.13336413855112994 1104 5 0.23982533698464437 1104 6 0.077843750712006 1104 7 0.10597650018975457 1104 8 0.16566231869104797 1104 9 0.3358411104367889 1104 10-11 0.3474028760894612 1104 12-13 0.2308268138846543 1104 14-15 0.29147602094644043 1104 16-17 0.3243755019483885 1104 18-19 0.29758448792721026 1104 20-21 0.3351658577005949 1104 22-23 0.23909375745572703 1104 24-25 0.12935196272312766 1104 26-27 0.23603633987648465 1104 28-29 0.18220302102012198 1104 30-31 0.4279527431299286 1104 32-33 0.459925062140087 1104 34-35 0.41267319501019983 1104 36-37 0.4396861817839337 1104 38-39 0.4454089857403929 1104 40-41 0.27498724797257523 1104 42-43 0.37716185043702666 1104 44-45 0.13111874890221742 1104 46-47 0.09496247215020048 1104 48-49 -0.031047218082065342 1104 50-51 0.04253889819802481 1104 52-53 0.19792398196528183 1104 54-55 0.33339877388942085 1104 56-57 0.3405663264263481 1104 58-59 0.28637501126837606 1104 60-61 0.37793774425423265 1104 62-63 0.237374417233422 1104 64-65 0.24931477995584217 1104 66-67 0.2629587533014721 1104 68-69 0.1700966376457096 1104 70-71 -0.01854377256501394 1104 72-73 0.09371629753060873 1104 74-75 0.202424870737957 1104 76-77 0.15292421753493457 1104 78-79 0.2283794483859083 1104 80-81 0.33215581603463207 1104 82-83 0.40479940064707165 1104 84-85 0.4863359744718174 1104 86-87 0.014933190289180942 1104 88-89 -0.08004148544636891 1104 90-91 0.1498419084492646 1104 92-93 0.13602875997482045 1104 94-95 0.13269269055025745 1104 96-97 0.34529068842327604 1104 98-99 0.038114618209441176 1104 100-101 0.28621308040819216 1105 1 0.20208078067047808 1105 2 0.1821531749625862 1105 3 0.1800477793140871 1105 4 -3.294879234090331E-4 1105 5 0.1649048456850224 1105 6 0.11860841846355186 1105 7 0.16849971132840125 1105 8 0.10117164703665793 1105 9 0.27607960277627086 1105 10-11 0.24191458125567067 1105 12-13 0.2091235552095938 1105 14-15 0.35249515030085377 1105 16-17 0.2333287555592065 1105 18-19 0.05326446456414402 1105 20-21 0.2102983722012297 1105 22-23 0.24313629376638346 1105 24-25 0.14120357011558582 1105 26-27 0.2332088011687503 1105 28-29 0.15106844368229844 1105 30-31 0.12148932726059769 1105 32-33 -0.059224127242551106 1105 34-35 0.27433312059950765 1105 36-37 0.18041227684325634 1105 38-39 0.20918247541269608 1105 40-41 0.1773308133385143 1105 42-43 0.10054669280385298 1105 44-45 0.2889603999822583 1105 46-47 0.1863232019421588 1105 48-49 0.05106703811487989 1105 50-51 0.1918451805670216 1105 52-53 0.2450113638838829 1105 54-55 0.2257860268384988 1105 56-57 0.23797520371622483 1105 58-59 0.3484386418618399 1105 60-61 0.33618132785147026 1105 62-63 0.3324839569270033 1105 64-65 0.31547960773763606 1105 66-67 0.17773969079513918 1105 68-69 0.045890036566685666 1105 70-71 -0.1468477726460442 1105 72-73 0.08279946599451904 1105 74-75 0.29659077214041574 1105 76-77 0.12823052016091907 1105 78-79 0.311503600124297 1105 80-81 0.2637475261975659 1105 82-83 0.3404366012655835 1105 84-85 0.3235895987856061 1105 86-87 0.1824281429215766 1105 88-89 0.1681374952217105 1105 90-91 0.22387024798348776 1105 92-93 0.15763790803318756 1105 94-95 0.1929842562133004 1105 96-97 0.4604896796249882 1105 98-99 0.49235238129714887 1105 100-101 0.9337202885217941 1106 1 0.1876222888761916 1106 2 0.24229498379388303 1106 3 0.20594708643448456 1106 4 0.10402062228547493 1106 5 0.22333092754619344 1106 6 0.21642330443754787 1106 7 0.2755406928683044 1106 8 0.2089215848821837 1106 9 0.31235936554384836 1106 10-11 0.16706654125190568 1106 12-13 0.37354080103294507 1106 14-15 0.4541447255859481 1106 16-17 0.40195537454042807 1106 18-19 0.06930163601297323 1106 20-21 0.2551269005326873 1106 22-23 0.28859734816978033 1106 24-25 0.31066872539634005 1106 26-27 0.23562909190233228 1106 28-29 0.352843821375032 1106 30-31 0.3674058004357832 1106 32-33 0.17660048030577258 1106 34-35 0.0956765708359626 1106 36-37 0.05365712680718815 1106 38-39 0.1365126970691577 1106 40-41 0.27790816908083826 1106 42-43 0.45538898736246125 1106 44-45 0.2578014628720382 1106 46-47 0.1423332099102339 1106 48-49 0.2605587946309953 1106 50-51 0.30950337577686327 1106 52-53 0.41000139720571127 1106 54-55 0.2787192347208318 1106 56-57 0.39398556308767496 1106 58-59 0.4844062705830936 1106 60-61 0.2685700010665357 1106 62-63 0.386185859688414 1106 64-65 0.30069431080617903 1106 66-67 0.35018650369832116 1106 68-69 0.2998848340397444 1106 70-71 0.06521977059633954 1106 72-73 0.23724608035479378 1106 74-75 0.28904174917357395 1106 76-77 0.3614815372540541 1106 78-79 0.39383433570819193 1106 80-81 0.504563379409344 1106 82-83 0.5621101949712468 1106 84-85 0.4802619142615008 1106 86-87 0.359894753012302 1106 88-89 0.4536149601626356 1106 90-91 0.4197095441928127 1106 92-93 0.3084540862930929 1106 94-95 0.41341651750279595 1106 96-97 0.544233056769535 1106 98-99 0.335951524927129 1106 100-101 0.5899533497810658 1107 1 0.1954031790100217 1107 2 0.21520950572801212 1107 3 0.25670243130747394 1107 4 0.15495951636162175 1107 5 0.08951557804456911 1107 6 0.09506535955019046 1107 7 0.12245267703519147 1107 8 0.05810798308819187 1107 9 0.057593071561868214 1107 10-11 0.1209278578583266 1107 12-13 0.22403997506152962 1107 14-15 0.23866195127964573 1107 16-17 0.2784347412158752 1107 18-19 0.25990950309142846 1107 20-21 0.2163022538649031 1107 22-23 0.2500959077075535 1107 24-25 0.27814061443685745 1107 26-27 0.3314976387512658 1107 28-29 0.35407280299616417 1107 30-31 0.3912472458458538 1107 32-33 0.38382753067008224 1107 34-35 0.2821944828440692 1107 36-37 0.3953738704847183 1107 38-39 0.6205399261944322 1107 40-41 0.5336760032800498 1107 42-43 0.4460678800021398 1107 44-45 0.15310260327122194 1107 46-47 0.3982007991111516 1107 48-49 0.6449028456417238 1107 50-51 0.67730405730304 1107 52-53 0.6734942587377901 1107 54-55 0.8582519024887887 1107 56-57 0.57243529055701 1107 58-59 0.5275682647254811 1107 60-61 0.3210770437696553 1107 62-63 0.5366483238763742 1107 64-65 0.6469558923618841 1107 66-67 0.5727598635265814 1107 68-69 0.5500105253419072 1107 70-71 0.5312591778122133 1107 72-73 0.49380796436765806 1107 74-75 0.36223398903247883 1107 76-77 0.5360647608568883 1107 78-79 0.6224927506411149 1107 80-81 0.9120665516183983 1107 82-83 0.8824274956807052 1107 84-85 0.862263717831933 1107 86-87 0.6902553872945028 1107 88-89 0.7381680976569491 1107 90-91 0.8819063887425997 1107 92-93 0.9128097886880084 1107 94-95 0.8299774438676408 1107 96-97 0.8112572453623557 1107 98-99 1.0646179202601864 1107 100-101 1.2410143439793124 1108 1 0.08263300206871804 1108 2 0.12565853382409387 1108 3 0.06886531298907528 1108 4 0.09402164258952439 1108 5 0.059297430179363175 1108 6 0.1218882099024512 1108 7 0.14246949919105134 1108 8 -0.026195710841172115 1108 9 0.12897148518627688 1108 10-11 0.21497374067426023 1108 12-13 0.14972925903958156 1108 14-15 0.22727227144076778 1108 16-17 0.13190836206917567 1108 18-19 0.22428974554653536 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8.0 5 30.0 6 99.0 7 205.0 8 430.0 9 878.0 10 1600.0 11 2783.0 12 4305.0 13 6428.0 14 7913.0 15 11564.0 16 16297.0 17 23545.0 18 20069.0 19 9658.0 20 6882.0 21 6681.0 22 6871.0 23 7130.0 24 8077.0 25 9044.0 26 10734.0 27 12988.0 28 15739.0 29 19256.0 30 23622.0 31 29219.0 32 37056.0 33 47781.0 34 66334.0 35 96244.0 36 136408.0 37 198184.0 38 136938.0 39 8704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.85316750450249 19.74432363707661 15.971232619202105 18.431276239218803 2 15.545612636399369 22.546175462209327 40.256899163238685 21.65131273815262 3 19.036884728277617 29.115140370110286 31.84201352928398 20.00596137232812 4 11.84879519533113 18.429550653528732 38.11432509113836 31.607329060001778 5 11.519256184652443 39.98219676914807 32.636898964138915 15.861648082060567 6 27.18768850237797 38.50263057120831 17.856258758887712 16.45342216752601 7 24.93763318308715 37.75877555928056 20.483697733374154 16.819893524258138 8 27.389262904550733 32.2982816161114 19.667498224225504 20.644957255112363 9 28.710358297236866 15.924276731847442 20.851482607513926 34.51388236340176 10-11 24.091905820533547 28.46690582053355 25.8269502829426 21.6142380759903 12-13 27.394365414480422 26.870271968831243 27.417756128316505 18.317606488371833 14-15 23.854941199888046 25.861844239064208 29.62012066172986 20.66309389931788 16-17 19.099705064822086 29.876509155721532 29.563538373400668 21.460247406055718 18-19 18.914919640236413 25.898411194037013 36.33969903168165 18.846970134044923 20-21 20.956462037214326 24.387503479591533 36.393117304798075 18.26291717839607 22-23 20.107114673629138 25.063227060676756 36.190937134669845 18.63872113102425 24-25 19.542905993788395 25.017999705941644 36.943531196206145 18.495563104063816 26-27 19.577982114889103 25.03809427575405 36.89481129692315 18.48911231243369 28-29 18.89844054699072 25.217690910229905 37.114506732302324 18.76936181047705 30-31 18.988136677279428 25.35998682414375 36.989145074866954 18.66273142370987 32-33 18.924790396774675 25.520591511795626 36.51751724695416 19.037100844475543 34-35 19.34139226782822 25.25030084600625 36.45061326106967 18.95769362509586 36-37 19.711858369521558 25.03775448595038 36.30018668829118 18.950200456236878 38-39 19.684331204655706 25.167810837207444 36.271292460499716 18.87656549763713 40-41 19.337345654219984 25.53047560299747 36.14226107568797 18.989917667094574 42-43 19.35538628020964 25.93166274127041 35.385350643471966 19.32760033504798 44-45 19.47860431702652 26.201236929238796 34.643213308154216 19.67694544558047 46-47 19.984823775949625 26.49372641976506 33.466453674121404 20.054996130163907 48-49 20.309866583677213 26.8479635924194 32.54427675904632 20.297893064857064 50-51 20.479484341125847 27.446820657679282 32.01713945333005 20.056555547864818 52-53 20.380384537273077 27.82226896996996 31.66789430024381 20.129452192513153 54-55 19.821275834700355 27.692343445845964 31.74597079969667 20.740409919757013 56-57 20.501806890788824 28.09605293005289 30.849458437975375 20.552681741182912 58-59 20.592984179140068 27.49276047634348 31.23407355315619 20.680181791360262 60-61 20.480948336879052 27.380832694665656 31.816360771372466 20.321858197082825 62-63 20.914867904395642 27.37025368257041 31.71173592600302 20.003142487030924 64-65 20.556214560254134 27.56686820759254 31.75507321351348 20.12184401863984 66-67 20.372804735234475 27.90301378112474 31.440293749437952 20.283887734202835 68-69 20.504009279542164 28.0754144673559 31.286879713530507 20.133696539571428 70-71 20.399039709973014 28.309155997619484 30.893572907373034 20.39823138503447 72-73 20.446211967356064 28.095581630560098 30.631326986013057 20.826879416070785 74-75 20.37795958205897 28.48296822601391 30.529680946621685 20.60939124530544 76-77 20.317992460410082 28.370965607062278 30.751564348712602 20.559477583815042 78-79 20.2636076632038 28.2303912182647 30.65508863629589 20.85091248223561 80-81 20.12492826021938 28.71758574419413 30.380331983412955 20.777154012173536 82-83 20.002192796310446 28.662626666828352 30.1992008924782 21.135979644383003 84-85 20.04017614450478 28.230157976318708 30.122559471000628 21.607106408175884 86-87 20.169885168619594 28.21121415908458 30.056882683444 21.56201798885182 88-89 20.38996468644697 27.8359713247887 30.059563203176705 21.714500785587624 90-91 19.75867483343471 27.92229378136045 30.602646049012733 21.716385336192108 92-93 19.659454767926267 28.278773414758106 30.378384713859756 21.68338710345587 94-95 19.48668750107355 28.19207464012149 30.14471930638198 22.176518552422987 96-97 19.65515359906718 27.990263588387037 29.868483672110475 22.486099140435307 98-99 19.542392986909057 27.96688262610994 29.868635898845298 22.622088488135702 100-101 19.455424136056564 27.977197268683828 30.132938468595725 22.43444012666388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3212.0 1 2234.0 2 1424.0 3 1578.5 4 1283.0 5 1192.0 6 2227.0 7 4753.5 8 7612.5 9 8498.0 10 6656.0 11 4747.5 12 4452.5 13 4511.5 14 4508.0 15 4521.0 16 4500.0 17 4545.0 18 4614.5 19 4680.5 20 4894.0 21 5266.0 22 5752.0 23 6304.5 24 6980.0 25 8082.5 26 9389.5 27 10543.5 28 11570.0 29 12672.5 30 14242.0 31 16062.0 32 18228.0 33 20034.5 34 21890.0 35 23187.5 36 24813.5 37 27197.5 38 27450.5 39 26883.5 40 27106.0 41 27895.0 42 28475.0 43 29655.0 44 31365.0 45 32281.0 46 32133.0 47 33269.5 48 43046.0 49 42565.0 50 31572.5 51 27661.5 52 24980.0 53 23873.0 54 22787.5 55 20970.0 56 19483.5 57 17892.5 58 16736.0 59 15775.0 60 13981.5 61 11705.0 62 9428.5 63 6736.0 64 4433.5 65 3241.0 66 2454.0 67 1823.0 68 1268.0 69 855.5 70 608.0 71 453.0 72 379.5 73 336.5 74 262.0 75 217.0 76 171.0 77 88.0 78 44.0 79 35.5 80 22.0 81 14.0 82 14.0 83 9.0 84 5.0 85 3.0 86 2.5 87 2.5 88 2.5 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025765149149901637 2 0.006062388035270974 3 4.041592023513982E-4 4 5.051990029392478E-4 5 1.0103980058784955E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011013338264075603 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.5363930205747343E-4 20-21 9.598781055845709E-4 22-23 0.0022228756129326903 24-25 0.011063858164369526 26-27 0.005961348234683124 28-29 3.031194017635487E-4 30-31 0.0020207960117569913 32-33 0.004193151724395757 34-35 0.0 36-37 0.009245141753788235 38-39 0.012882574574950819 40-41 7.577985044088717E-4 42-43 0.0 44-45 0.0 46-47 3.031194017635487E-4 48-49 0.002525995014696239 50-51 0.0063149875367405975 52-53 0.0 54-55 0.0027785945161658628 56-57 0.0025765149149901637 58-59 3.031194017635487E-4 60-61 0.0018692363108752169 62-63 0.004748870627628929 64-65 1.0103980058784955E-4 66-67 0.0022228756129326903 68-69 5.051990029392478E-4 70-71 8.083184047027964E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0018692363108752169 80-81 0.0021218358123448406 82-83 0.010609179061724204 84-85 0.00676966663938592 86-87 0.004092111923807907 88-89 4.041592023513982E-4 90-91 7.072786041149469E-4 92-93 2.020796011756991E-4 94-95 0.0 96-97 0.0022228756129326903 98-99 0.0017176766099934425 100-101 3.031194017635487E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 989709.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.12945548347067 #Duplication Level Percentage of deduplicated Percentage of total 1 75.01744099737415 36.105485869670076 2 13.467787576254103 12.963945652243225 3 4.793760807648317 6.921632921703483 4 2.127097527718061 4.095041830772278 5 1.1622214768625931 2.796854341629586 6 0.741393576098868 2.1409721469948955 7 0.4895809488847611 1.649428513943311 8 0.3505504121290939 1.3497440364223616 9 0.26403143470672435 1.1436920264658765 >10 1.3944526380832338 12.426055697140717 >50 0.11813999668515926 3.9180164001162003 >100 0.06487810500708806 5.531995287526185 >500 0.005071903930466367 1.634506312173243 >1k 0.002958610626105381 2.9278717300696306 >5k 0.0 0.0 >10k+ 6.339879913082959E-4 4.394757233128903 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18631 1.8824725247522254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14307 1.4455764270103637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10270 1.037678752037215 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3396 0.3431311627963371 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3115 0.3147389788311514 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3013 0.30443291917119075 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2984 0.3015027649541431 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2793 0.2822041630418638 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2549 0.25755045169842855 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.16479591475878264 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1625 0.16418967595525552 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1461 0.1476191486588482 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1433 0.14479003424238843 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1359 0.13731308899888756 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1204 0.12165191990777087 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1139 0.11508433286956064 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1084 0.10952714383722893 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 1.0103980058784956E-4 12-13 0.0 0.0 0.0 1.0103980058784956E-4 1.5155970088177433E-4 14-15 0.0 1.0103980058784956E-4 0.0 1.0103980058784956E-4 2.0207960117569912E-4 16-17 0.0 1.0103980058784956E-4 0.0 2.0207960117569912E-4 2.0207960117569912E-4 18-19 0.0 1.0103980058784956E-4 0.0 3.0311940176354867E-4 2.0207960117569912E-4 20-21 0.0 1.0103980058784956E-4 0.0 4.0415920235139824E-4 2.0207960117569912E-4 22-23 0.0 1.0103980058784956E-4 0.0 0.001364037307935969 2.0207960117569912E-4 24-25 0.0 1.0103980058784956E-4 0.0 0.0023744353138144647 2.0207960117569912E-4 26-27 0.0 1.0103980058784956E-4 0.0 0.0037384726217504338 2.0207960117569912E-4 28-29 0.0 1.0103980058784956E-4 0.0 0.005658228832919576 2.0207960117569912E-4 30-31 0.0 1.0103980058784956E-4 0.0 0.010053460158491031 2.0207960117569912E-4 32-33 0.0 1.0103980058784956E-4 0.0 0.014297131783180712 2.0207960117569912E-4 34-35 0.0 1.0103980058784956E-4 0.0 0.01859132330816432 3.0311940176354867E-4 36-37 0.0 1.0103980058784956E-4 0.0 0.025310470047256315 3.0311940176354867E-4 38-39 0.0 2.0207960117569912E-4 0.0 0.03475769140222025 3.0311940176354867E-4 40-41 0.0 2.0207960117569912E-4 0.0 0.05279329580715139 3.0311940176354867E-4 42-43 0.0 2.0207960117569912E-4 0.0 0.06865654449944378 3.0311940176354867E-4 44-45 0.0 2.0207960117569912E-4 0.0 0.08649006930319922 3.0311940176354867E-4 46-47 0.0 2.0207960117569912E-4 0.0 0.12155088010718301 3.0311940176354867E-4 48-49 0.0 2.0207960117569912E-4 0.0 0.1447395143420945 3.0311940176354867E-4 50-51 0.0 2.0207960117569912E-4 0.0 0.17535457392021292 3.0311940176354867E-4 52-53 0.0 2.0207960117569912E-4 0.0 0.210213305123021 4.0415920235139824E-4 54-55 0.0 2.0207960117569912E-4 0.0 0.25189222286550894 4.0415920235139824E-4 56-57 0.0 2.0207960117569912E-4 0.0 0.3623287249080285 4.0415920235139824E-4 58-59 0.0 2.0207960117569912E-4 0.0 0.44998075191798803 4.0415920235139824E-4 60-61 0.0 2.0207960117569912E-4 0.0 0.5189404158191954 4.0415920235139824E-4 62-63 0.0 2.0207960117569912E-4 0.0 0.5995196567880053 4.0415920235139824E-4 64-65 0.0 2.0207960117569912E-4 0.0 0.668933999791858 4.0415920235139824E-4 66-67 0.0 2.0207960117569912E-4 0.0 0.7899796808961017 4.0415920235139824E-4 68-69 0.0 2.0207960117569912E-4 0.0 0.9501782847281373 4.0415920235139824E-4 70-71 0.0 2.0207960117569912E-4 0.0 1.028938809286366 4.0415920235139824E-4 72-73 0.0 2.0207960117569912E-4 0.0 1.1205313885192516 4.5467910264532306E-4 74-75 0.0 2.0207960117569912E-4 0.0 1.2971489599468127 5.557189032331726E-4 76-77 0.0 2.0207960117569912E-4 0.0 1.4764440860899517 6.062388035270973E-4 78-79 0.0 2.0207960117569912E-4 0.0 1.64684770978136 6.062388035270973E-4 80-81 0.0 3.0311940176354867E-4 0.0 1.801337564880182 6.062388035270973E-4 82-83 0.0 3.0311940176354867E-4 0.0 2.0429742479860242 6.062388035270973E-4 84-85 0.0 3.0311940176354867E-4 0.0 2.4541557164782777 6.062388035270973E-4 86-87 0.0 3.0311940176354867E-4 0.0 2.8043596653157645 6.062388035270973E-4 88-89 0.0 3.0311940176354867E-4 0.0 3.2355470143244123 6.062388035270973E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTATC 45 9.630374E-4 42.216923 2 GGTATCA 11220 0.0 31.328804 1 GTATCAA 16730 0.0 31.288921 1 TAATACC 255 0.0 26.072523 4 TATCAAC 20615 0.0 25.34244 2 ATCAACG 20795 0.0 25.052029 3 TCAACGC 20990 0.0 24.819294 4 CAACGCA 21145 0.0 24.604874 5 GAGTACT 12060 0.0 24.453413 12-13 AACGCAG 21475 0.0 24.271025 6 AGTACTT 12160 0.0 23.998468 12-13 GTACATG 10415 0.0 23.397152 1 TACATGG 10570 0.0 22.691095 2 GTACTTT 13055 0.0 22.390734 14-15 ACGCAGA 23300 0.0 22.308794 7 CGCAGAG 23375 0.0 22.176233 8 GTGTACG 130 2.9355695E-4 21.929188 9 ACATGGG 10420 0.0 21.693739 3 GCAGAGT 23700 0.0 21.6917 9 GGATATG 395 0.0 21.646133 1 >>END_MODULE