FastQCFastQC Report
Thu 26 May 2016
SRR936820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013763
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155981.5386239190027649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108281.0680997432338721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74710.7369572572682176No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32350.31910811501307507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32150.31713526731593084No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25180.24838152507045533No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23460.23141503487501516No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT19660.1939309286292753No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.19294450478070319No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17970.17726036558840674No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13670.13484414009980636No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11650.1149183783586499No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.10761884187921636No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.10465957033350004No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG10540.10396907363949957No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10360.10219351071206978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG756.870383E-638.0410422
TCACGCA753.0587637E-431.7008694
GTATCAA159000.030.2563881
GGTATCA107900.027.0948891
ATCACGC908.890602E-426.4173893
TATCAAC191250.024.962882
ATCAACG194000.024.6580473
GTACATG113850.024.55151
GAGTACT92450.024.5258312-13
TCAACGC195900.024.4188944
CAACGCA197450.024.200735
TACATGG114300.023.962862
AACGCAG200850.023.7390216
AGTACTT94300.023.6414112-13
ACATGGG116400.022.7543583
TAGCGCT1053.454261E-822.61610662-63
GTACTTT100750.022.49978414-15
CTTACAC4550.021.9467543
ACGCAGA217500.021.7687647
CATGGGA94050.021.6900674
Produced by FastQC (version 0.11.5)