##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936820_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1013763 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.812288473735972 33.0 31.0 34.0 30.0 34.0 2 31.83857568287657 33.0 31.0 34.0 30.0 34.0 3 31.82055963770625 33.0 31.0 34.0 30.0 34.0 4 35.4722099741261 37.0 35.0 37.0 33.0 37.0 5 35.50260662501985 37.0 35.0 37.0 33.0 37.0 6 35.6164202086681 37.0 35.0 37.0 33.0 37.0 7 35.57946975772444 37.0 35.0 37.0 33.0 37.0 8 35.54842699920987 37.0 35.0 37.0 33.0 37.0 9 37.13642833680061 39.0 37.0 39.0 33.0 39.0 10-11 37.02635329953846 39.0 37.0 39.0 33.0 39.0 12-13 36.92993234118823 39.0 37.0 39.0 33.0 39.0 14-15 37.946537307043165 40.0 37.5 41.0 33.0 41.0 16-17 37.92915849167902 40.0 37.5 41.0 33.0 41.0 18-19 38.02973229443174 40.0 38.0 41.0 33.0 41.0 20-21 38.03526169331491 40.0 38.0 41.0 33.0 41.0 22-23 37.927413014678976 40.0 38.0 41.0 33.0 41.0 24-25 37.85598902307541 40.0 37.0 41.0 33.0 41.0 26-27 37.81643539959537 40.0 37.0 41.0 33.0 41.0 28-29 37.71389368126475 40.0 37.0 41.0 33.0 41.0 30-31 37.44542905984929 39.5 36.5 41.0 32.5 41.0 32-33 37.128089602796706 39.0 35.5 41.0 32.0 41.0 34-35 36.894193711942535 39.0 35.0 41.0 31.0 41.0 36-37 36.651077717375756 39.0 35.0 41.0 31.0 41.0 38-39 36.43676135349189 39.0 35.0 41.0 30.5 41.0 40-41 36.17401059221929 39.0 35.0 41.0 30.0 41.0 42-43 35.83967159977233 39.0 35.0 40.0 29.5 41.0 44-45 35.39371135068058 38.0 35.0 40.0 27.5 41.0 46-47 34.75276667228928 38.0 35.0 40.0 24.0 41.0 48-49 34.25054228651075 38.0 34.0 40.0 21.0 41.0 50-51 33.42811337561146 37.5 33.5 39.5 18.0 40.5 52-53 33.563812745187974 38.0 33.5 39.5 18.0 40.5 54-55 33.92289617987636 38.0 34.0 40.0 17.0 41.0 56-57 33.81594711979032 38.0 34.0 40.0 14.5 41.0 58-59 33.60359867148436 38.0 34.0 40.0 12.5 41.0 60-61 33.31557622442327 37.5 33.5 40.0 8.5 41.0 62-63 33.005933832661086 37.0 33.0 40.0 7.5 41.0 64-65 32.65871954293064 36.5 33.0 40.0 4.5 41.0 66-67 32.30324247383264 36.0 32.5 39.0 2.0 41.0 68-69 31.918339394907882 35.5 32.0 39.0 2.0 41.0 70-71 31.512331777742922 35.0 31.5 38.5 2.0 40.0 72-73 31.136076183486672 35.0 31.0 37.5 2.0 40.0 74-75 30.759122694357558 35.0 31.0 37.0 2.0 39.5 76-77 30.309559038946972 35.0 31.0 36.5 2.0 39.0 78-79 29.866321319677283 35.0 30.0 36.0 2.0 39.0 80-81 29.45452043524966 35.0 30.0 36.0 2.0 37.5 82-83 29.06703835117281 34.0 29.0 35.0 2.0 37.0 84-85 28.7031209464145 34.0 29.0 35.0 2.0 36.5 86-87 28.340604756733086 34.0 29.0 35.0 2.0 36.0 88-89 27.97613594104342 34.0 28.5 35.0 2.0 36.0 90-91 27.597879879222262 34.0 27.0 35.0 2.0 35.5 92-93 27.248535900402757 34.0 26.5 35.0 2.0 35.0 94-95 26.90085700503964 34.0 25.5 35.0 2.0 35.0 96-97 26.444817970275103 34.0 25.0 35.0 2.0 35.0 98-99 25.942090015121877 33.5 23.5 35.0 2.0 35.0 100-101 23.98262513033125 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17089872763672176 1101 2 0.14674715878788902 1101 3 0.21737969082252562 1101 4 0.15739917625717936 1101 5 0.10991914561099492 1101 6 0.11535366196725505 1101 7 0.08095185388653192 1101 8 0.1172804900906641 1101 9 0.24718113790144258 1101 10-11 0.15786951975260166 1101 12-13 0.2289318651353014 1101 14-15 0.4762741942101343 1101 16-17 0.5814971976409851 1101 18-19 0.5009562044534519 1101 20-21 0.5112903586039863 1101 22-23 0.6314589923524565 1101 24-25 0.5918399470063562 1101 26-27 0.6380136981457696 1101 28-29 0.5684522024177241 1101 30-31 0.6586349294896578 1101 32-33 0.5909608744482711 1101 34-35 0.7048391060282171 1101 36-37 0.9054240217840857 1101 38-39 0.8193594547996454 1101 40-41 0.8539982953999257 1101 42-43 0.8266692094864467 1101 44-45 0.9867173267350751 1101 46-47 0.9270982717480862 1101 48-49 0.830651213282259 1101 50-51 0.8831503386267414 1101 52-53 0.8414036370223101 1101 54-55 0.8484258597462002 1101 56-57 0.9238514728591412 1101 58-59 0.8548776164275154 1101 60-61 0.8373230981102111 1101 62-63 0.8145915246452375 1101 64-65 0.8583068194451968 1101 66-67 0.9398404701319194 1101 68-69 0.8018622169952998 1101 70-71 0.7907065712073127 1101 72-73 0.6572844286645214 1101 74-75 0.7036974505863434 1101 76-77 0.7728795242406257 1101 78-79 0.8402907729395075 1101 80-81 0.8859307780052674 1101 82-83 0.5925104918153075 1101 84-85 0.7088250416963149 1101 86-87 0.680905226721439 1101 88-89 0.693949505928348 1101 90-91 0.8449420164318298 1101 92-93 1.002466715766328 1101 94-95 0.9822046965306939 1101 96-97 1.057955915356171 1101 98-99 1.1123084936861183 1101 100-101 1.2072976671631679 1102 1 0.2851761626987148 1102 2 0.18567417139386677 1102 3 0.31152826321700644 1102 4 0.36651684386187355 1102 5 0.29416809496888163 1102 6 0.10974276305915964 1102 7 0.03386204414481142 1102 8 -0.08947328665834675 1102 9 0.03531407808664255 1102 10-11 0.04376888061378281 1102 12-13 0.16813390320421462 1102 14-15 0.08423009699480133 1102 16-17 0.2746924519209557 1102 18-19 0.05339646252375729 1102 20-21 0.04594494680316075 1102 22-23 0.17476364450001114 1102 24-25 0.002658477633879386 1102 26-27 0.02244824010202251 1102 28-29 0.04273701978753763 1102 30-31 0.10142937447550793 1102 32-33 0.16262704234920733 1102 34-35 0.05921057927295692 1102 36-37 -0.028122271581580094 1102 38-39 0.07655353963926359 1102 40-41 -0.07624148299284883 1102 42-43 0.13774292993673498 1102 44-45 0.1894724971534032 1102 46-47 0.23677012989049473 1102 48-49 0.21927840587511582 1102 50-51 0.1641191630373413 1102 52-53 0.31477644615429057 1102 54-55 -0.04515722441987435 1102 56-57 0.12602106068316488 1102 58-59 -0.011085632443368354 1102 60-61 0.34668465784697133 1102 62-63 0.20471544565109667 1102 64-65 0.3318637550630186 1102 66-67 -0.08760645012621637 1102 68-69 0.09465480778000668 1102 70-71 0.0881256094547389 1102 72-73 0.1899149191481584 1102 74-75 0.3521171968798775 1102 76-77 0.325822141458481 1102 78-79 0.17147567646498985 1102 80-81 0.08710685898297754 1102 82-83 0.1953776904795923 1102 84-85 0.007182391965262269 1102 86-87 0.2189969863928205 1102 88-89 0.34741656022367096 1102 90-91 0.1309460749762863 1102 92-93 0.39128536540768977 1102 94-95 0.4423031502994199 1102 96-97 0.36245757076970264 1102 98-99 0.44621432959018037 1102 100-101 0.5675745554514293 1103 1 0.21379911719159495 1103 2 0.21938237163776364 1103 3 0.1976359421452294 1103 4 0.3541222978489955 1103 5 0.2556521520427921 1103 6 0.3663819212165649 1103 7 0.2732892417023365 1103 8 0.3084861026253449 1103 9 0.3111302055043126 1103 10-11 0.22293446926605753 1103 12-13 0.3575468750017379 1103 14-15 0.6404573126065429 1103 16-17 0.510591016207286 1103 18-19 0.5037191414479523 1103 20-21 0.5057021658726057 1103 22-23 0.48710410844515906 1103 24-25 0.5449218806774567 1103 26-27 0.6009143888381914 1103 28-29 0.6088477131260959 1103 30-31 0.5657783303530906 1103 32-33 0.44639226653187336 1103 34-35 0.7225041691374514 1103 36-37 0.46520693507236643 1103 38-39 0.5331194331177471 1103 40-41 0.2091042916340271 1103 42-43 0.3309650202449177 1103 44-45 0.25546753063028405 1103 46-47 0.08208372931404284 1103 48-49 0.10958530347463125 1103 50-51 0.017223884780676713 1103 52-53 0.03080367901426939 1103 54-55 0.08431693829541587 1103 56-57 0.07125913864999944 1103 58-59 0.2532382570809091 1103 60-61 0.17043434250257405 1103 62-63 -0.024279393581700504 1103 64-65 0.17343513331751836 1103 66-67 0.33380408435130704 1103 68-69 0.3121477372145911 1103 70-71 0.37098691353378754 1103 72-73 0.16119419781749755 1103 74-75 0.15582870975117302 1103 76-77 -0.05203701465170596 1103 78-79 0.1888302976608074 1103 80-81 0.33579974134762836 1103 82-83 0.14104173525406338 1103 84-85 0.03977227821559737 1103 86-87 0.013690657017715324 1103 88-89 0.12363890764332552 1103 90-91 0.4593813945158729 1103 92-93 0.3528318868668272 1103 94-95 0.26679515364912376 1103 96-97 0.2653351236488284 1103 98-99 0.2002573872984037 1103 100-101 0.3492861118462933 1104 1 0.0971142469570303 1104 2 0.1518873244266885 1104 3 0.12008165059499731 1104 4 0.11642625487911573 1104 5 0.18674337319486511 1104 6 -0.04217942953545162 1104 7 0.0740949268893587 1104 8 0.21982999875626064 1104 9 0.10241329699024959 1104 10-11 0.3418947854880443 1104 12-13 0.11373135988805672 1104 14-15 -0.07613677619850279 1104 16-17 0.2135891674813024 1104 18-19 0.24002154762865047 1104 20-21 0.27145179825510013 1104 22-23 -0.03469880128877634 1104 24-25 -0.0468985706764542 1104 26-27 0.14998127867586675 1104 28-29 0.2686277572874758 1104 30-31 0.349999855953925 1104 32-33 0.058321750165212904 1104 34-35 0.05083861359765507 1104 36-37 -0.02007772617578496 1104 38-39 0.39420847929806513 1104 40-41 0.39030071522396526 1104 42-43 0.3709230338337619 1104 44-45 0.41418964016375526 1104 46-47 -0.02380075131688386 1104 48-49 -0.126600079769581 1104 50-51 -0.09619498168078877 1104 52-53 -0.12124457698936908 1104 54-55 0.11514952693733704 1104 56-57 0.19252799825557076 1104 58-59 -0.013438102394630391 1104 60-61 -0.02647979812353185 1104 62-63 -0.061126251929572106 1104 64-65 -0.0424958255140524 1104 66-67 -0.1826996958441569 1104 68-69 0.0032899283567822124 1104 70-71 0.03307431902889846 1104 72-73 0.08613284942957833 1104 74-75 0.010249812650975798 1104 76-77 0.06571761070940951 1104 78-79 0.23718559348060708 1104 80-81 0.051870182063098014 1104 82-83 -0.0029118432836909847 1104 84-85 -0.009883272454150216 1104 86-87 0.10032663766992655 1104 88-89 0.11385744502210571 1104 90-91 -0.006138210963165136 1104 92-93 0.22889750481884974 1104 94-95 0.2858994435444906 1104 96-97 0.3473942072292111 1104 98-99 0.2796804996829785 1104 100-101 0.5803378525329208 1105 1 0.2067689784148321 1105 2 0.2174001896518334 1105 3 0.23110718729189017 1105 4 0.293357007417292 1105 5 0.238490060011884 1105 6 0.35440954202154984 1105 7 0.27284715505335555 1105 8 0.34524656854623004 1105 9 0.2608212253789901 1105 10-11 0.1988587667327124 1105 12-13 0.13500790410257935 1105 14-15 0.38633077010855743 1105 16-17 0.26946037960251346 1105 18-19 0.25905781826338625 1105 20-21 0.26477215011125566 1105 22-23 0.29427023691939524 1105 24-25 0.2074511929444114 1105 26-27 0.20613101129619338 1105 28-29 0.32426404342248816 1105 30-31 0.22310591655998024 1105 32-33 0.22651422478351435 1105 34-35 -0.12561744313333634 1105 36-37 -0.08277147956646758 1105 38-39 0.09147607210594089 1105 40-41 0.0708861410939221 1105 42-43 0.02299280442974805 1105 44-45 -0.18813879120879307 1105 46-47 -0.11076696072554881 1105 48-49 0.10958530347462414 1105 50-51 -0.18410573886904302 1105 52-53 -0.035133304417101385 1105 54-55 0.07732644315836978 1105 56-57 0.03791763632423795 1105 58-59 0.05791884947702641 1105 60-61 0.06326677244144463 1105 62-63 -0.08046284031986772 1105 64-65 0.1115478077495311 1105 66-67 0.1213478124872367 1105 68-69 0.0941990192658757 1105 70-71 0.08392731833138711 1105 72-73 0.14610678959853018 1105 74-75 0.24639160138227822 1105 76-77 0.3382365985416982 1105 78-79 0.3041055927055929 1105 80-81 -0.11411592581878693 1105 82-83 -0.28563194713551354 1105 84-85 -0.17095288804742026 1105 86-87 -0.02877609037781781 1105 88-89 0.03629796273116881 1105 90-91 0.16958158288322522 1105 92-93 0.3332094865285349 1105 94-95 0.3599156607032903 1105 96-97 -0.20503546028997377 1105 98-99 -0.05713555084790656 1105 100-101 -0.04136591790806321 1106 1 0.0876496919042431 1106 2 0.1720127871992112 1106 3 0.08017351951039586 1106 4 0.11552813339276469 1106 5 0.21390812021255812 1106 6 0.22974399018208658 1106 7 0.26162920633540665 1106 8 0.3124427781337431 1106 9 0.32615009940352024 1106 10-11 0.3084671836780828 1106 12-13 0.34375929763658064 1106 14-15 0.3172532896180371 1106 16-17 0.30151166165379095 1106 18-19 0.4021110512622741 1106 20-21 0.23765397842787195 1106 22-23 0.11620446211448865 1106 24-25 0.09411507254306883 1106 26-27 0.03305408821927358 1106 28-29 0.022828703400186612 1106 30-31 0.235445660149729 1106 32-33 0.2131362983621159 1106 34-35 0.16674599053443728 1106 36-37 0.0683015416152557 1106 38-39 0.00944569306469134 1106 40-41 0.08207621913182095 1106 42-43 0.09969291591247043 1106 44-45 0.30545095238095143 1106 46-47 0.3919643659188168 1106 48-49 0.44836735475667666 1106 50-51 0.4608357327028614 1106 52-53 0.5116336966977286 1106 54-55 0.7063328534147857 1106 56-57 0.6717178035483187 1106 58-59 0.53035474691292 1106 60-61 0.6709869508138055 1106 62-63 0.6622977505385492 1106 64-65 0.3206057787640262 1106 66-67 0.3914356051293808 1106 68-69 0.4812182500351021 1106 70-71 0.5022313874506743 1106 72-73 0.5266948609474724 1106 74-75 0.662570531148166 1106 76-77 0.7594461860556301 1106 78-79 0.45717136751049736 1106 80-81 0.6594347988188929 1106 82-83 0.5218164921064066 1106 84-85 0.583583065129833 1106 86-87 0.8428794280168326 1106 88-89 0.7907642392083147 1106 90-91 0.9645648604751997 1106 92-93 1.0052412591037871 1106 94-95 1.093674021907301 1106 96-97 1.1675676612261938 1106 98-99 1.0778491202780707 1106 100-101 1.2071220976995178 1107 1 0.1255769389069954 1107 2 0.24933984059112646 1107 3 0.09098221710515375 1107 4 0.1697503390670505 1107 5 0.15089430682362348 1107 6 0.17817369455322307 1107 7 0.12392814892234583 1107 8 0.21842795945774185 1107 9 0.2178015626546923 1107 10-11 0.17597314093476513 1107 12-13 0.22352743003660436 1107 14-15 0.0032281388249728593 1107 16-17 0.13545545866027453 1107 18-19 0.1595078231738043 1107 20-21 0.2945336790757267 1107 22-23 0.21945209782823127 1107 24-25 0.22920610687176435 1107 26-27 0.30680864924391926 1107 28-29 0.1729301629280684 1107 30-31 0.3386472202063189 1107 32-33 0.20957590667248382 1107 34-35 0.22996272297915255 1107 36-37 0.1733428700834878 1107 38-39 0.1953660892222615 1107 40-41 0.37702232457527174 1107 42-43 0.35663172371647534 1107 44-45 0.0955716799109183 1107 46-47 0.22474975574680656 1107 48-49 0.39290250257111126 1107 50-51 0.3659831748057769 1107 52-53 0.17347141730239457 1107 54-55 0.2600970949815391 1107 56-57 0.3518591953124073 1107 58-59 0.3813800466373962 1107 60-61 0.2916679397184154 1107 62-63 0.2562376380953353 1107 64-65 0.33962804401087254 1107 66-67 0.24058157653454515 1107 68-69 0.2926501325282196 1107 70-71 0.2607344405447236 1107 72-73 0.4818948944570387 1107 74-75 0.3778602027543876 1107 76-77 0.4770936281585456 1107 78-79 0.3933537898799955 1107 80-81 0.5666528530299288 1107 82-83 0.5385829117951388 1107 84-85 0.6529888287756762 1107 86-87 0.5964212032793235 1107 88-89 0.6862902463570215 1107 90-91 0.5252441739013705 1107 92-93 0.6242281531390823 1107 94-95 0.47752240972471327 1107 96-97 0.40972456047408556 1107 98-99 0.7536668468399874 1107 100-101 0.8588618028153121 1108 1 -0.06974796483611811 1108 2 -0.013762022759642889 1108 3 0.02714680914007417 1108 4 -0.04685069358261984 1108 5 0.04603815020070101 1108 6 0.03613477754522165 1108 7 -0.10627040312132152 1108 8 -0.16166803852014056 1108 9 -0.05162293218864278 1108 10-11 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2316 52-53 -1.2435509884833778 2316 54-55 -1.1857764699266298 2316 56-57 -1.236196291668783 2316 58-59 -1.3326912269317646 2316 60-61 -1.492015400225796 2316 62-63 -1.5898893591521386 2316 64-65 -2.2120112221910873 2316 66-67 -1.8126406016151648 2316 68-69 -1.5834514105335522 2316 70-71 -1.3903508989353384 2316 72-73 -1.7972990594110634 2316 74-75 -2.2747687532541576 2316 76-77 -2.16637024918057 2316 78-79 -2.3638185487337147 2316 80-81 -2.2475912279241825 2316 82-83 -2.7397114081213516 2316 84-85 -2.6720961649046977 2316 86-87 -3.087310308225913 2316 88-89 -2.851144419229712 2316 90-91 -3.208483592981562 2316 92-93 -3.2133962399778433 2316 94-95 -3.220094253752091 2316 96-97 -3.588543204984962 2316 98-99 -3.9924657904558956 2316 100-101 -4.254909531130107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1707.0 3 560.0 4 476.0 5 555.0 6 690.0 7 873.0 8 1147.0 9 1475.0 10 1972.0 11 2906.0 12 4306.0 13 6236.0 14 7351.0 15 9964.0 16 13626.0 17 19226.0 18 16190.0 19 7617.0 20 6882.0 21 6708.0 22 7066.0 23 7665.0 24 8585.0 25 9377.0 26 10972.0 27 13109.0 28 15483.0 29 18476.0 30 22296.0 31 27835.0 32 35104.0 33 45972.0 34 63526.0 35 93848.0 36 132865.0 37 186491.0 38 173024.0 39 31594.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.70044900577293 19.46928504465387 15.076528346573248 18.75373760299995 2 15.103892233885308 22.16785369636905 40.94709774691303 21.781156322832608 3 19.004928490027083 29.671976674773926 30.83429123971998 20.48880359547901 4 11.673145990566736 17.883328744017852 38.66059100513905 31.782934260276363 5 11.827040712043582 40.16807751590476 32.395553874758455 15.609327897293204 6 27.270599458996625 38.134516074555364 18.482306779923817 16.112577686524197 7 25.67963843422833 37.52952428517424 20.393841379698348 16.39699590089908 8 26.954330658904702 32.59106913295985 19.998775673823708 20.45582453431174 9 29.13786533514187 16.360637094338056 20.670718103327427 33.83077946719265 10-11 24.061995606999186 28.555492485007033 26.027740072558554 21.354771835435226 12-13 27.102077200547285 26.992846975018708 27.16698354784511 18.738092276588898 14-15 23.944491276975043 26.170012615170187 28.75868896991904 21.12680713793573 16-17 19.494990668262915 30.43779713760932 28.533797074443672 21.533415119684093 18-19 19.397444977186414 27.37422916239966 34.138648359864874 19.089677500549058 20-21 21.898597307331478 25.631823648520935 33.72177206231799 18.747806981829594 22-23 20.861678340171657 26.440192775649642 33.429148830774636 19.268980053404064 24-25 20.02352865736208 26.5955709892148 34.24776120139199 19.133139152031134 26-27 20.16362361143856 26.544914863834418 34.12968357420967 19.161777950517347 28-29 19.477019343694494 26.874748135400434 34.4005240263414 19.24770849456367 30-31 19.582197652383478 27.059047517581476 34.193244806941635 19.16551002309341 32-33 19.403539422302433 27.233447091905717 33.73070736655399 19.632306119237857 34-35 19.789798499349903 26.976434703174725 33.80926591259295 19.42450088488242 36-37 20.187985167165337 26.824830299389262 33.57078101247217 19.41640352097323 38-39 20.077752727190223 26.91884842706588 33.61463866240056 19.388760183343337 40-41 19.750831490645822 27.203000123804927 33.62596016638198 19.42020821916727 42-43 19.66185176087842 27.62444918602346 32.88454386634666 19.829155186751464 44-45 19.782933042661227 27.88336288554539 32.322803867846126 20.010900203947255 46-47 20.164696331274794 28.082667772796793 31.38488050983261 20.367755386095805 48-49 20.374842171717173 28.401988636363633 30.651929450757574 20.571239741161616 50-51 20.640576240439522 28.84595560908652 30.263769878672463 20.2496982718015 52-53 20.52345810056417 29.223558031411628 29.964999541284854 20.287984326739345 54-55 19.935608366275634 29.10300589656496 30.097292828526072 20.86409290863333 56-57 20.554869660479472 29.58094742963629 29.229850263322877 20.63433264656136 58-59 20.675210167759346 28.901198957496888 29.599818094649972 20.823772780093794 60-61 20.467407355527694 28.842376899030427 30.205792209252657 20.484423536189226 62-63 21.00371371311628 28.85050491996699 29.992389083094146 20.153392283822587 64-65 20.56748502568716 29.055888318063083 30.19992384605065 20.176702810199103 66-67 20.42327729102534 29.38822124871734 29.69946325676849 20.48903820348883 68-69 20.41278258153433 29.62553397689361 29.642607420829304 20.319076020742756 70-71 20.378483352210615 29.79925922087408 29.243600847873775 20.578656579041528 72-73 20.368050349208854 29.691038945665472 29.054916545002563 20.88599416012311 74-75 20.263740231706244 30.11702714354915 29.080929468997475 20.538303155747126 76-77 20.441588835762815 29.847946073198212 29.21012074725561 20.50034434378336 78-79 20.236545336951796 29.816315747669847 29.143058424448608 20.80408049092975 80-81 20.038896005015015 30.427683293721604 28.896868822235877 20.6365518790275 82-83 20.090895725473594 30.21281361344057 28.729344459475325 20.966946201610508 84-85 20.140491310176913 29.860100606023536 28.69787314643908 21.301534937360465 86-87 20.482943584713713 29.839920034023038 28.62607321070699 21.051063170556258 88-89 20.485941315769086 29.534610945448424 28.638502749265864 21.34094498951662 90-91 19.97934540832539 29.745177799225132 29.12993149346612 21.145545298983357 92-93 20.060187668287888 30.190890336371005 28.776692303251505 20.9722296920896 94-95 19.807438844889568 30.06194433592594 28.634186091479126 21.496430727705366 96-97 19.970935151152506 29.865069448315428 28.311935086531065 21.852060314000997 98-99 19.982276572312767 29.86229804568618 28.424095177569797 21.731330204431252 100-101 20.005320971042664 29.921379938063502 28.769276180115362 21.30402291077847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2320.0 1 1607.5 2 1085.5 3 1280.5 4 1152.0 5 1296.0 6 2227.0 7 4209.5 8 6696.5 9 7488.0 10 6043.0 11 4771.5 12 4637.0 13 4660.5 14 4626.5 15 4528.0 16 4338.5 17 4268.5 18 4341.5 19 4485.5 20 4671.5 21 5031.5 22 5595.0 23 6213.5 24 6920.0 25 8073.0 26 9523.0 27 10711.5 28 11578.5 29 12821.5 30 14725.5 31 16737.5 32 19044.5 33 20988.0 34 23030.0 35 24451.0 36 26145.5 37 28604.5 38 28787.5 39 28102.5 40 28197.5 41 29111.0 42 29632.0 43 30407.5 44 32209.5 45 33412.0 46 33114.0 47 33962.5 48 43704.0 49 43356.0 50 32596.5 51 28293.5 52 25427.5 53 24574.5 54 23372.0 55 21672.5 56 20381.0 57 18758.0 58 17296.0 59 16126.0 60 14462.5 61 12458.0 62 10206.0 63 7189.5 64 4679.5 65 3363.5 66 2458.0 67 1846.0 68 1335.0 69 915.5 70 641.0 71 446.0 72 348.5 73 333.0 74 268.5 75 198.5 76 142.5 77 78.5 78 49.5 79 24.0 80 13.0 81 9.0 82 6.5 83 5.0 84 3.0 85 4.0 86 3.5 87 2.0 88 1.0 89 1.0 90 1.0 91 2.0 92 2.5 93 2.5 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04073930494602782 2 0.030480496920877957 3 0.02643615914173234 4 0.053266887822893516 5 0.10022066301492558 6 0.11837086182865225 7 0.06056642430232707 8 0.09479533184777902 9 0.07782884165233886 10-11 0.07773019926748165 12-13 0.07546142441576582 14-15 0.06875374224547552 16-17 0.054647881210894464 18-19 0.06515329519818734 20-21 0.05962932164618358 22-23 0.035215331394024045 24-25 0.010406771602435678 26-27 0.0033538410851451476 28-29 0.007250215287004951 30-31 0.005326688782289352 32-33 0.00655971859300448 34-35 0.006806324555147504 36-37 0.006510397400575874 38-39 0.003600447047288173 40-41 0.00705293051729053 42-43 0.003551125854859568 44-45 0.0020221688895728095 46-47 0.001824884119858389 48-49 2.9592715457163064E-4 50-51 0.001923526504715599 52-53 0.006115827861147033 54-55 0.012034370952579646 56-57 0.008236639135577054 58-59 0.004833476858003301 60-61 0.0021701324668586246 62-63 0.008631208675005895 64-65 0.002663344391144676 66-67 0.02397009952030208 68-69 0.04868001692703324 70-71 0.06318044750104314 72-73 0.0042416225488600395 74-75 0.014993642498295953 76-77 0.02377281475058766 78-79 0.025400414100731628 80-81 0.07945644100248284 82-83 0.029000861148019804 84-85 0.010456092794864282 86-87 0.03274927177259379 88-89 0.04858137454217603 90-91 0.04231758310374318 92-93 0.04266283145074342 94-95 0.010949304719150335 96-97 0.016522599463582713 98-99 0.04093658971574224 100-101 0.07792748403719607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1013763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.33141603042377 #Duplication Level Percentage of deduplicated Percentage of total 1 75.65236922843745 38.07690869323714 2 13.297192451889927 13.385330506653654 3 4.639623366520346 7.005564416544326 4 2.0703390183895864 4.168123778343418 5 1.1259602427360655 2.8335586705432925 6 0.6859952495296214 2.071626737938184 7 0.4598731351687555 1.620224626117576 8 0.3186348616561697 1.2829875027050561 9 0.23477122025225292 1.0634731162637752 >10 1.3385467385601222 12.380783849839352 >50 0.110733508322463 3.857691805842468 >100 0.05889373100645773 5.283201101705049 >500 0.003926248628235516 1.2689760736809073 >1k 0.002552061608353086 2.3522941202305816 >5k 1.9631243141177582E-4 0.7381858013291333 >10k+ 3.9262486282355165E-4 2.6110691990260575 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15598 1.5386239190027649 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10828 1.0680997432338721 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7471 0.7369572572682176 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3235 0.31910811501307507 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3215 0.31713526731593084 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2518 0.24838152507045533 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2346 0.23141503487501516 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1966 0.1939309286292753 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1956 0.19294450478070319 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1797 0.17726036558840674 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1367 0.13484414009980636 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1165 0.1149183783586499 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.10761884187921636 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1061 0.10465957033350004 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1054 0.10396907363949957 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1036 0.10219351071206978 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.4524834700023577E-4 1.9728476971442042E-4 14-15 0.0 0.0 0.0 3.9456953942884084E-4 1.9728476971442042E-4 16-17 0.0 0.0 0.0 4.93211924286051E-4 1.9728476971442042E-4 18-19 0.0 0.0 0.0 4.93211924286051E-4 2.466059621430255E-4 20-21 0.0 0.0 9.864238485721021E-5 4.93211924286051E-4 2.9592715457163064E-4 22-23 0.0 0.0 9.864238485721021E-5 9.86423848572102E-4 2.9592715457163064E-4 24-25 0.0 0.0 9.864238485721021E-5 0.0017262417350011787 2.9592715457163064E-4 26-27 0.0 0.0 9.864238485721021E-5 0.0025647020062874656 2.9592715457163064E-4 28-29 0.0 0.0 9.864238485721021E-5 0.0054253311671465625 2.9592715457163064E-4 30-31 0.0 0.0 9.864238485721021E-5 0.010653377564578704 2.9592715457163064E-4 32-33 0.0 0.0 9.864238485721021E-5 0.016078708731725264 2.9592715457163064E-4 34-35 0.0 0.0 9.864238485721021E-5 0.020714900820014145 2.9592715457163064E-4 36-37 0.0 0.0 9.864238485721021E-5 0.025153808138588606 2.9592715457163064E-4 38-39 0.0 9.864238485721021E-5 9.864238485721021E-5 0.03487008304702381 2.9592715457163064E-4 40-41 0.0 9.864238485721021E-5 9.864238485721021E-5 0.05454923882603725 2.9592715457163064E-4 42-43 0.0 9.864238485721021E-5 9.864238485721021E-5 0.07186097736847764 3.4524834700023577E-4 44-45 0.0 9.864238485721021E-5 9.864238485721021E-5 0.09149081195506248 3.9456953942884084E-4 46-47 0.0 9.864238485721021E-5 9.864238485721021E-5 0.12611428903994326 3.9456953942884084E-4 48-49 0.0 9.864238485721021E-5 9.864238485721021E-5 0.14919660709653043 3.9456953942884084E-4 50-51 0.0 9.864238485721021E-5 9.864238485721021E-5 0.17893728613097934 3.9456953942884084E-4 52-53 0.0 9.864238485721021E-5 9.864238485721021E-5 0.2121797698278592 3.9456953942884084E-4 54-55 0.0 9.864238485721021E-5 9.864238485721021E-5 0.25316568073603 3.9456953942884084E-4 56-57 0.0 9.864238485721021E-5 9.864238485721021E-5 0.36004470472881733 3.9456953942884084E-4 58-59 0.0 9.864238485721021E-5 9.864238485721021E-5 0.43895861261458546 3.9456953942884084E-4 60-61 0.0 9.864238485721021E-5 9.864238485721021E-5 0.5027802356172005 3.9456953942884084E-4 62-63 0.0 9.864238485721021E-5 9.864238485721021E-5 0.5808556832316824 3.9456953942884084E-4 64-65 0.0 9.864238485721021E-5 9.864238485721021E-5 0.651089061250016 3.9456953942884084E-4 66-67 0.0 9.864238485721021E-5 9.864238485721021E-5 0.7701010985802401 3.9456953942884084E-4 68-69 0.0 9.864238485721021E-5 9.864238485721021E-5 0.9260547090394895 3.9456953942884084E-4 70-71 0.0 9.864238485721021E-5 9.864238485721021E-5 0.9966826565972521 3.9456953942884084E-4 72-73 0.0 9.864238485721021E-5 9.864238485721021E-5 1.0813178228047384 3.9456953942884084E-4 74-75 0.0 9.864238485721021E-5 9.864238485721021E-5 1.245360108822279 3.9456953942884084E-4 76-77 0.0 9.864238485721021E-5 9.864238485721021E-5 1.4235082558744008 3.9456953942884084E-4 78-79 0.0 9.864238485721021E-5 9.864238485721021E-5 1.5904111710528004 3.9456953942884084E-4 80-81 0.0 2.9592715457163064E-4 9.864238485721021E-5 1.7319136721304684 3.9456953942884084E-4 82-83 0.0 2.9592715457163064E-4 9.864238485721021E-5 1.9598762235354812 3.9456953942884084E-4 84-85 0.0 2.9592715457163064E-4 9.864238485721021E-5 2.3581941735888963 3.9456953942884084E-4 86-87 0.0 2.9592715457163064E-4 9.864238485721021E-5 2.676809076677685 4.438907318574459E-4 88-89 0.0 2.9592715457163064E-4 9.864238485721021E-5 3.0962858182829716 4.93211924286051E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 75 6.870383E-6 38.041042 2 TCACGCA 75 3.0587637E-4 31.700869 4 GTATCAA 15900 0.0 30.256388 1 GGTATCA 10790 0.0 27.094889 1 ATCACGC 90 8.890602E-4 26.417389 3 TATCAAC 19125 0.0 24.96288 2 ATCAACG 19400 0.0 24.658047 3 GTACATG 11385 0.0 24.5515 1 GAGTACT 9245 0.0 24.52583 12-13 TCAACGC 19590 0.0 24.418894 4 CAACGCA 19745 0.0 24.20073 5 TACATGG 11430 0.0 23.96286 2 AACGCAG 20085 0.0 23.739021 6 AGTACTT 9430 0.0 23.64141 12-13 ACATGGG 11640 0.0 22.754358 3 TAGCGCT 105 3.454261E-8 22.616106 62-63 GTACTTT 10075 0.0 22.499784 14-15 CTTACAC 455 0.0 21.946754 3 ACGCAGA 21750 0.0 21.768764 7 CATGGGA 9405 0.0 21.690067 4 >>END_MODULE