FastQCFastQC Report
Thu 26 May 2016
SRR936820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013763
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194321.9168188225453089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147611.45606024287728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108181.0671133193853No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37150.36645645974453595No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33160.32709814818650906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31200.3077642407544959No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30980.3055941082876372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28750.28359685646447935No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25070.24729645883702603No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.17410380927297603No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15910.15694003430782147No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15540.15329026606810467No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.14332738519752644No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13460.13277265001780497No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT12090.11925864329236716No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG11370.11215639158264801No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10820.10673106041550146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG702.0527482E-433.9297372
GGTATCA118700.031.6190741
GTATCAA172800.030.0778181
ATCACGC804.4936666E-429.6870563
CGTAGCT650.005835733429.2389858
TCACGCA1109.476515E-525.90874
GTAAGGT3900.025.576544
TATCAAC214900.024.1818432
GAGTACT125450.024.16024812-13
ATCAACG216100.024.0683573
TCAACGC219000.023.7496434
CAACGCA220300.023.5663725
GTACATG108150.023.545671
GTCGGGA4050.023.4575942
AGTACTT126550.023.36837612-13
TACATGG109000.023.0971222
AACGCAG225350.023.022866
CATGGGT7350.022.6187064
GTACTTT135750.022.2882514-15
ACATGGG108050.022.0241953
Produced by FastQC (version 0.11.5)