##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936820_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1013763 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11123605813193 31.0 31.0 34.0 31.0 34.0 2 32.19448825810372 33.0 31.0 34.0 30.0 34.0 3 32.275530868654705 34.0 31.0 34.0 30.0 34.0 4 36.00231415034875 37.0 35.0 37.0 35.0 37.0 5 35.871385126503924 37.0 35.0 37.0 35.0 37.0 6 35.99593889301543 37.0 35.0 37.0 35.0 37.0 7 35.945945945945944 37.0 35.0 37.0 35.0 37.0 8 35.90910794732102 37.0 35.0 37.0 35.0 37.0 9 37.56600507219143 39.0 37.0 39.0 35.0 39.0 10-11 37.496096720831204 39.0 37.0 39.0 35.0 39.0 12-13 37.37345760300978 39.0 37.0 39.0 34.0 39.0 14-15 38.41177375777178 40.0 38.0 41.0 34.0 41.0 16-17 38.4403662394465 40.0 38.0 41.0 33.5 41.0 18-19 38.70425089493304 40.0 38.0 41.0 34.5 41.0 20-21 38.766092272059645 40.0 38.0 41.0 35.0 41.0 22-23 38.66975318688885 40.0 38.0 41.0 34.0 41.0 24-25 38.559208611874766 40.0 38.0 41.0 34.0 41.0 26-27 38.314330371102514 40.0 38.0 41.0 34.0 41.0 28-29 38.11755213003434 40.0 37.0 41.0 34.0 41.0 30-31 37.86749417763323 40.0 37.0 41.0 33.0 41.0 32-33 37.517365498642185 40.0 35.5 41.0 33.0 41.0 34-35 37.288943273723746 39.0 35.0 41.0 33.0 41.0 36-37 37.033212397769496 39.0 35.0 41.0 33.0 41.0 38-39 36.78511989488668 39.0 35.0 41.0 32.0 41.0 40-41 36.33842525323966 39.0 35.0 41.0 30.5 41.0 42-43 35.975470104945636 39.0 35.0 41.0 30.0 41.0 44-45 35.47171824183759 39.0 35.0 40.5 28.0 41.0 46-47 34.69001038704313 38.0 35.0 40.0 23.5 41.0 48-49 33.98768745752213 38.0 34.0 40.0 17.0 41.0 50-51 33.604790271493435 38.0 34.0 40.0 10.5 41.0 52-53 33.30672898892542 38.0 33.0 40.0 7.5 41.0 54-55 32.914771006635675 38.0 33.0 40.0 3.5 41.0 56-57 32.719402365247106 37.5 33.0 40.0 2.0 41.0 58-59 32.417801300698486 37.0 32.5 40.0 2.0 41.0 60-61 32.040708232594795 36.5 32.0 39.5 2.0 41.0 62-63 31.665024764170717 36.0 31.0 39.0 2.0 40.0 64-65 31.34271866304057 35.5 31.0 39.0 2.0 40.0 66-67 31.033369239161424 35.0 31.0 39.0 2.0 40.0 68-69 30.604524430266245 35.0 30.5 38.0 2.0 40.0 70-71 30.162065985836925 35.0 30.0 37.5 2.0 39.5 72-73 29.761267179804353 35.0 29.0 37.0 2.0 39.0 74-75 29.288740070410935 34.0 29.0 36.0 2.0 39.0 76-77 27.87360310052744 32.5 27.0 35.0 2.0 37.0 78-79 28.491770759043288 34.0 28.5 35.0 2.0 37.0 80-81 28.35421888547915 34.0 28.5 35.0 2.0 37.0 82-83 28.016437273800683 34.0 27.5 35.0 2.0 36.5 84-85 27.59245504126704 34.0 27.0 35.0 2.0 36.0 86-87 27.194999718869205 33.0 26.0 35.0 2.0 36.0 88-89 26.802077014055556 33.0 25.5 35.0 2.0 35.0 90-91 26.337843263169006 33.0 25.0 35.0 2.0 35.0 92-93 25.885485562207343 33.0 24.0 35.0 2.0 35.0 94-95 25.372317297040826 32.0 21.0 34.0 2.0 35.0 96-97 24.635744251861627 32.0 14.0 34.0 2.0 35.0 98-99 23.83899787228376 31.5 2.0 34.0 2.0 35.0 100-101 20.929378957409178 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2086985532970118 1101 2 0.36510346639538227 1101 3 0.35684122997038514 1101 4 0.21728410065006187 1101 5 0.02936962992855996 1101 6 0.10840751346648148 1101 7 0.06446815265463357 1101 8 0.18527057650198486 1101 9 0.2690330724202852 1101 10-11 0.33842960627976026 1101 12-13 0.20962302429808943 1101 14-15 0.3011966723801862 1101 16-17 0.45207563669729467 1101 18-19 0.3080068572607715 1101 20-21 0.14968615205447833 1101 22-23 0.299921105732345 1101 24-25 0.13229885783187711 1101 26-27 0.14461193829571073 1101 28-29 0.23794934172939008 1101 30-31 0.2646887497889949 1101 32-33 0.37788650112837985 1101 34-35 0.3008450855086622 1101 36-37 0.34414039484033054 1101 38-39 0.2817047535682846 1101 40-41 0.45762790113860774 1101 42-43 0.5981139201590366 1101 44-45 0.3035744012406596 1101 46-47 -0.11493260476398603 1101 48-49 0.13282157221558322 1101 50-51 0.13555736241482208 1101 52-53 0.27687731393451287 1101 54-55 -0.09313743599986424 1101 56-57 0.02117179802402802 1101 58-59 0.1933209513097225 1101 60-61 0.21428808374319175 1101 62-63 0.06039265671627092 1101 64-65 -0.09946383461061004 1101 66-67 0.10837622246486944 1101 68-69 -0.2510904536246592 1101 70-71 -0.43957438737792387 1101 72-73 -0.12767573847538927 1101 74-75 -0.4058608750072459 1101 76-77 -0.19975753621501724 1101 78-79 -0.14960207422748084 1101 80-81 -0.028162786610391066 1101 82-83 0.18972281333252994 1101 84-85 -0.11907239054502838 1101 86-87 -0.25460296419468165 1101 88-89 -0.15577868937884531 1101 90-91 0.1381019458558761 1101 92-93 -0.04950305767029661 1101 94-95 -0.08980186185663186 1101 96-97 -0.2550131886116631 1101 98-99 -0.15220943391069142 1101 100-101 0.2245890065117635 1102 1 -0.09222190664836205 1102 2 0.02203744562315535 1102 3 0.14056289626765306 1102 4 -0.027177228044607205 1102 5 -0.06416748729748889 1102 6 0.03953127147512703 1102 7 -0.002505592719565186 1102 8 0.108880076566237 1102 9 0.19929096161494897 1102 10-11 0.16553397365651534 1102 12-13 0.22031529676389283 1102 14-15 0.2596500616442725 1102 16-17 0.24103391199700042 1102 18-19 0.14982366391243573 1102 20-21 0.09773919773078177 1102 22-23 -0.15251647883863484 1102 24-25 -0.12139380530978627 1102 26-27 -0.011097405756913759 1102 28-29 -0.10716794296121179 1102 30-31 0.02015152821024202 1102 32-33 -0.013854455760153428 1102 34-35 0.03021968502277872 1102 36-37 0.13948962715793556 1102 38-39 0.1574517163436937 1102 40-41 0.27661690443218845 1102 42-43 0.3275454393362196 1102 44-45 0.1029351761759969 1102 46-47 0.2936065520833253 1102 48-49 0.41541260702368277 1102 50-51 0.36830660801368964 1102 52-53 0.29438916572951257 1102 54-55 0.5272631749378505 1102 56-57 0.31309722636605386 1102 58-59 -0.04351702341967467 1102 60-61 0.16889292760652097 1102 62-63 0.4239286269248659 1102 64-65 0.4612289121857174 1102 66-67 0.49012691606546 1102 68-69 0.241676868279324 1102 70-71 0.10391180399805222 1102 72-73 0.02004113622114545 1102 74-75 0.07804940235537572 1102 76-77 0.19948301749256458 1102 78-79 -0.12111550769917301 1102 80-81 0.0979069365271954 1102 82-83 0.5337042920191308 1102 84-85 0.41932250945315985 1102 86-87 0.24189906378392223 1102 88-89 0.08268192695761556 1102 90-91 0.10234130511295092 1102 92-93 0.1661426915951374 1102 94-95 0.2926577402749224 1102 96-97 0.6653083384911511 1102 98-99 0.46106198922749897 1102 100-101 0.6253521612064716 1103 1 0.15087094283181557 1103 2 0.2196699245242968 1103 3 0.12706575310653534 1103 4 0.07128759886456493 1103 5 -0.014731963841910556 1103 6 -0.025616261342861435 1103 7 0.11484846428180617 1103 8 0.15681726076847013 1103 9 0.24680661196592268 1103 10-11 0.04929782765266566 1103 12-13 0.26824321205734947 1103 14-15 0.2821058066348314 1103 16-17 0.26874351627908055 1103 18-19 0.24644639595150863 1103 20-21 0.06549185810909819 1103 22-23 0.09702591916508396 1103 24-25 0.199357588854987 1103 26-27 0.2922429477673205 1103 28-29 0.03262372420920201 1103 30-31 0.13570902889330938 1103 32-33 0.12389120054709934 1103 34-35 0.13301067435386216 1103 36-37 0.1262769991966124 1103 38-39 0.2751483199737166 1103 40-41 0.33474747031115726 1103 42-43 0.13214999141821693 1103 44-45 0.16534515693696505 1103 46-47 0.12331654807753978 1103 48-49 0.08762208113804348 1103 50-51 0.06515767760546254 1103 52-53 0.10513940732563043 1103 54-55 0.29717823143386823 1103 56-57 0.1813380761396104 1103 58-59 0.3174360460802248 1103 60-61 0.23703149698671666 1103 62-63 0.46468361581426265 1103 64-65 0.3889737383211376 1103 66-67 0.4852055285017549 1103 68-69 0.2905953478236505 1103 70-71 0.1245971874125118 1103 72-73 0.3419829806695134 1103 74-75 0.29544037379343635 1103 76-77 0.5139371956345435 1103 78-79 0.5119912612640078 1103 80-81 0.4795977789259247 1103 82-83 0.6824444861181433 1103 84-85 0.38350986392121555 1103 86-87 0.3030460549752334 1103 88-89 0.6265766579456731 1103 90-91 0.36551181998874327 1103 92-93 0.4697429775611255 1103 94-95 0.6308282350009158 1103 96-97 0.9446079636242395 1103 98-99 0.7782093898627807 1103 100-101 1.1087656504509091 1104 1 0.08509932432185963 1104 2 0.07839841712703333 1104 3 0.098001511060545 1104 4 0.04056139471037312 1104 5 -0.07211575651982116 1104 6 0.05958352872508499 1104 7 0.13742798077613116 1104 8 0.09387074143249663 1104 9 0.020526806578608614 1104 10-11 0.0890797158118346 1104 12-13 0.24109389079038834 1104 14-15 0.23791723658418817 1104 16-17 0.22339180916266344 1104 18-19 0.10370087237433978 1104 20-21 0.12753647339914664 1104 22-23 0.08738630252749857 1104 24-25 0.07103425566349841 1104 26-27 0.10260314200175458 1104 28-29 0.15659711390267717 1104 30-31 0.2139595478132037 1104 32-33 -0.052552200541335026 1104 34-35 0.07074383368945547 1104 36-37 -0.12756466286005974 1104 38-39 -0.04326990818082521 1104 40-41 0.07573197600154202 1104 42-43 0.05816549045603381 1104 44-45 0.14066040963558635 1104 46-47 0.08038567908176475 1104 48-49 0.3760848016494407 1104 50-51 0.34876335522736923 1104 52-53 0.35065589219302495 1104 54-55 0.46000796424331725 1104 56-57 0.346388880642742 1104 58-59 0.29547710576428443 1104 60-61 0.08033305681971825 1104 62-63 0.2595778696334641 1104 64-65 0.13254811526265442 1104 66-67 -0.16422330500415327 1104 68-69 0.04317812677559374 1104 70-71 0.13692478543990916 1104 72-73 0.20884279428389263 1104 74-75 0.006020192892524534 1104 76-77 0.1097978934222219 1104 78-79 0.09674896468296623 1104 80-81 0.13520400501896646 1104 82-83 0.4133212834249598 1104 84-85 0.3594870645434476 1104 86-87 0.3508020515141901 1104 88-89 0.25851351757015806 1104 90-91 0.35440291537070934 1104 92-93 0.40390871040839826 1104 94-95 0.4290546873855803 1104 96-97 0.43652037845754776 1104 98-99 0.27647414066302645 1104 100-101 0.8766370623385491 1105 1 0.04273030161396463 1105 2 0.18477871628226694 1105 3 0.03935720353516814 1105 4 0.09083071192342373 1105 5 -0.008530737531991406 1105 6 0.07020121453458472 1105 7 0.17463347562228648 1105 8 0.12975386938723688 1105 9 0.20454408938105928 1105 10-11 0.21542630187709477 1105 12-13 0.23573903145534558 1105 14-15 0.2923062352666719 1105 16-17 0.17621285853103075 1105 18-19 0.1345972724363449 1105 20-21 0.16045759639379753 1105 22-23 0.04453774017765966 1105 24-25 0.06762177407006931 1105 26-27 0.0845186848987538 1105 28-29 0.1782708525681045 1105 30-31 0.152367766832036 1105 32-33 0.2082132222285651 1105 34-35 0.1745956510962614 1105 36-37 0.21465468535353693 1105 38-39 -0.025391314055418945 1105 40-41 0.07447015704470772 1105 42-43 0.013008840839660252 1105 44-45 0.020337132103229294 1105 46-47 -0.0773223151831317 1105 48-49 0.10027200617552268 1105 50-51 -0.011729359645144655 1105 52-53 0.12860724071662588 1105 54-55 0.3362043914307904 1105 56-57 0.06952739494174409 1105 58-59 0.1580938421821756 1105 60-61 0.17820273828914424 1105 62-63 0.21297533533911306 1105 64-65 0.2588753259888463 1105 66-67 0.18223682054804158 1105 68-69 0.23144103075141587 1105 70-71 -0.05113225555831136 1105 72-73 0.11811534717856631 1105 74-75 0.03575891644518592 1105 76-77 0.01046537385312618 1105 78-79 0.2278376649852234 1105 80-81 0.1243174863798906 1105 82-83 0.32749095326918365 1105 84-85 -0.09326617113817548 1105 86-87 0.28424776181411815 1105 88-89 0.4880798006051137 1105 90-91 0.3325475329015184 1105 92-93 0.2083316159342452 1105 94-95 0.297341132398369 1105 96-97 0.36061990957434276 1105 98-99 0.46540065000194275 1105 100-101 0.7316417124199255 1106 1 -0.07577582993565102 1106 2 0.03921015440935349 1106 3 0.004254082019002681 1106 4 0.0648552381218579 1106 5 -0.014731963841910556 1106 6 0.051207346084197525 1106 7 0.11713626269029476 1106 8 0.1248765003794361 1106 9 0.1794326066608818 1106 10-11 0.11647842004877162 1106 12-13 0.1725701351342721 1106 14-15 0.26995394987090293 1106 16-17 0.2435832598688208 1106 18-19 0.03617893352887336 1106 20-21 0.02071400665020917 1106 22-23 0.14903188733484996 1106 24-25 0.0675660327099834 1106 26-27 0.21226394688314798 1106 28-29 0.26445396293598833 1106 30-31 0.19335160183761246 1106 32-33 0.2233330661527546 1106 34-35 0.09000116949091108 1106 36-37 0.11909309115063849 1106 38-39 0.31877674614523954 1106 40-41 0.3674397780034653 1106 42-43 0.4095041028240587 1106 44-45 0.21113980768851093 1106 46-47 0.47915761792723544 1106 48-49 0.20066916336615748 1106 50-51 0.21341696142508937 1106 52-53 0.23262061864304684 1106 54-55 0.4487531651208698 1106 56-57 0.5517459010732964 1106 58-59 0.3884241097406971 1106 60-61 0.13038361900262885 1106 62-63 0.17219356276386222 1106 64-65 0.06888090012485293 1106 66-67 0.16683130215339403 1106 68-69 0.37218796497660733 1106 70-71 0.3794767188006638 1106 72-73 0.4124018577694244 1106 74-75 0.39326309704719264 1106 76-77 0.5441261876769836 1106 78-79 0.40568047434977217 1106 80-81 0.5922801396686275 1106 82-83 0.8210110201588137 1106 84-85 0.8281385111360713 1106 86-87 0.6797536146569314 1106 88-89 0.7835229444178182 1106 90-91 0.46890483501968916 1106 92-93 0.7400623851650145 1106 94-95 0.5501142650628061 1106 96-97 0.5199008460291914 1106 98-99 0.3177208841156549 1106 100-101 0.7039800624756687 1107 1 0.19443509418379534 1107 2 0.22842007324173608 1107 3 0.0834185767691693 1107 4 0.04875016700710688 1107 5 0.044244877322732634 1107 6 0.1143458895349383 1107 7 0.051575722069252095 1107 8 0.06685840521225828 1107 9 0.11947281814349253 1107 10-11 0.13010424847978186 1107 12-13 0.17705995194862822 1107 14-15 0.4316380849517003 1107 16-17 0.24656514608522428 1107 18-19 0.17268853960527508 1107 20-21 0.25035554734571974 1107 22-23 0.1379602327068028 1107 24-25 0.26325772606019626 1107 26-27 0.19435820185373132 1107 28-29 0.2353899968923585 1107 30-31 0.3223406367620285 1107 32-33 0.2344633625262773 1107 34-35 0.16408384504381246 1107 36-37 0.3619536913442474 1107 38-39 0.28149502375340774 1107 40-41 0.4233988963294806 1107 42-43 0.3667375155874737 1107 44-45 0.22899621918602264 1107 46-47 0.2282421551228495 1107 48-49 0.3728976427763868 1107 50-51 0.30342226710682496 1107 52-53 0.3430667613193492 1107 54-55 0.6185298610773842 1107 56-57 0.3862779771772509 1107 58-59 0.3041503365632465 1107 60-61 0.18987287732417713 1107 62-63 0.3635103957372827 1107 64-65 0.39139002217415 1107 66-67 0.4457737960099699 1107 68-69 0.30317919663943016 1107 70-71 0.009290461447175602 1107 72-73 0.39107813671796876 1107 74-75 0.2247202047990733 1107 76-77 0.03501484282628908 1107 78-79 0.33931384243376783 1107 80-81 0.15985358147367634 1107 82-83 0.1754840821887491 1107 84-85 0.049106193018563005 1107 86-87 0.3175193641542897 1107 88-89 0.4755332393020275 1107 90-91 0.3526592098800698 1107 92-93 0.4563652558180813 1107 94-95 0.7888671506090112 1107 96-97 0.7501604521055931 1107 98-99 0.4050458897005811 1107 100-101 1.0100853197546904 1108 1 0.10903744156166795 1108 2 0.16152691290018595 1108 3 0.06409786470956647 1108 4 0.09271457280349438 1108 5 0.004028061264442329 1108 6 0.06934058410637789 1108 7 0.06139405612079685 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-1.6714928033430176 2316 100-101 -2.0607828131396424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 15.0 5 43.0 6 113.0 7 236.0 8 439.0 9 933.0 10 1734.0 11 3043.0 12 4724.0 13 6591.0 14 8386.0 15 12157.0 16 16737.0 17 24403.0 18 21024.0 19 10062.0 20 7248.0 21 7031.0 22 7367.0 23 7757.0 24 8684.0 25 9958.0 26 11519.0 27 13794.0 28 16879.0 29 20720.0 30 25508.0 31 31594.0 32 39498.0 33 51177.0 34 70611.0 35 100857.0 36 141300.0 37 199029.0 38 125976.0 39 6610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.928957408582214 19.710801816565553 16.04185638555548 18.318384389296746 2 15.497382922012456 22.587661754754244 40.39977271965009 21.515182603583217 3 18.961353848339158 29.174007311380777 31.942211288251087 19.922427552028978 4 11.817752275433213 18.41012149782543 38.139584893116044 31.632541333625312 5 11.456127180915761 39.99459425866867 32.70493234438518 15.844346216030392 6 27.105546365373367 38.60547287679665 17.875874341438777 16.41310641639121 7 24.95911269114447 37.73469512568038 20.421361226796822 16.884830956378323 8 27.327590373686945 32.27588696766404 19.72019101111404 20.676331647534976 9 28.61381665519126 15.93155000877918 20.907767306330282 34.54686602969928 10-11 24.074413842288582 28.47736601158259 25.830840147056065 21.61737999907276 12-13 27.38480964582804 26.99929219864802 27.349986805793613 18.26591134973032 14-15 23.810352897772642 25.859999970406484 29.639141570952187 20.690505560868687 16-17 18.997388936072827 29.830640889438655 29.631185987257375 21.54078418723114 18-19 18.82505489475792 25.918562418249717 36.49815833759142 18.758224349400944 20-21 20.87615073698955 24.30026066520181 36.600156349799384 18.223432248009253 22-23 19.97271511263154 24.981011059947836 36.44500408385516 18.601269743565464 24-25 19.42341297261626 24.944747688921968 37.2398850416544 18.391954296807373 26-27 19.491073590780474 25.024475677957785 37.04914814776454 18.435302583497204 28-29 18.84554757428272 25.17499176335274 37.359853693470455 18.61960696889408 30-31 18.92597184943621 25.34029156716116 37.15750130947766 18.576235273924972 32-33 18.879628551869175 25.4248531706653 36.724793393321114 18.970724884144406 34-35 19.198964649528538 25.191538850796487 36.69792643842792 18.91157006124706 36-37 19.644704075427143 25.01680646986204 36.48791882388743 18.850570630823384 38-39 19.590080929223554 25.112081080161218 36.49258358419732 18.80525440641791 40-41 19.299143781565657 25.49212831439394 36.23540088383838 18.97332702020202 42-43 19.21588181853155 25.88553734945939 35.590468383636015 19.30811244837304 44-45 19.424001988649113 26.082425362773098 34.90483633348738 19.588736315090404 46-47 19.90484932091949 26.452845617875226 33.73992180598869 19.90238325521659 48-49 20.183711577558068 26.823527787066602 32.767569934984806 20.225190700390524 50-51 20.42455219504207 27.369012395061702 32.22005809121672 19.986377318679498 52-53 20.308633663737467 27.828324004188374 31.86991773681799 19.993124595256166 54-55 19.803691789895666 27.62623019384215 31.85290842139632 20.71716959486586 56-57 20.42086905227522 28.025286076234057 31.039610479605862 20.514234391884862 58-59 20.530835438942432 27.4292596722796 31.402634054098577 20.637270834679395 60-61 20.368200192948503 27.27044948320184 32.025505503372656 20.335844820477007 62-63 20.87367603358775 27.39122822160023 31.836944057409895 19.898151687402127 64-65 20.500462386407072 27.55344570350551 31.89805304496862 20.0480388651188 66-67 20.365233654447493 27.841495341115568 31.56643275246064 20.226838251976304 68-69 20.491979137125313 27.997533939162278 31.41223905993761 20.098247863774798 70-71 20.406063580294102 28.234464356613888 31.048204328944546 20.311267734147467 72-73 20.42821793531898 28.127775867945008 30.772831228708586 20.671174968027426 74-75 20.3535942667321 28.441082830036525 30.708396939034703 20.49692596419667 76-77 20.373815550395456 28.284301443534083 30.873715921488476 20.468167084581985 78-79 20.15083421947163 28.258851683136673 30.783655335275935 20.806658762115763 80-81 19.99877681512251 28.73902462843293 30.6021620779149 20.660036478529655 82-83 19.942590103697857 28.53972207494359 30.372597131971247 21.14509068938731 84-85 19.933750303948592 28.2227552317383 30.320876003276975 21.52261846103613 86-87 20.155736904457758 28.173898976188667 30.220732500066582 21.44963161928699 88-89 20.30989131855476 27.823485747120202 30.242443223994258 21.62417971033078 90-91 19.70525277435265 27.882811344019732 30.754327990135632 21.657607891491985 92-93 19.5794283279228 28.30834228512976 30.52069369270727 21.59153569424017 94-95 19.45814751574086 28.10277155508733 30.35374145633644 22.085339472835365 96-97 19.577987316537133 27.94233552089694 30.038786262245242 22.44089090032069 98-99 19.542425538232234 27.83093803899055 30.05148662132056 22.57514980145666 100-101 19.403529089140672 27.917644064686815 30.361747419965155 22.317079426207354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3439.0 1 2419.5 2 1572.0 3 1697.5 4 1373.0 5 1276.0 6 2342.5 7 5114.0 8 8128.5 9 8934.5 10 7062.5 11 5040.5 12 4558.0 13 4534.5 14 4599.5 15 4599.5 16 4541.5 17 4641.5 18 4776.5 19 4887.5 20 5133.0 21 5438.5 22 5957.5 23 6636.0 24 7314.5 25 8368.0 26 9708.0 27 10893.5 28 11762.5 29 12990.5 30 14669.0 31 16504.5 32 18716.5 33 20490.5 34 22446.0 35 23933.0 36 25625.0 37 27997.5 38 28104.0 39 27666.0 40 28167.0 41 28840.0 42 29136.5 43 29921.5 44 31891.5 45 33234.5 46 32838.0 47 33796.0 48 43922.5 49 43410.5 50 32140.0 51 28048.5 52 25333.5 53 24274.5 54 23005.0 55 21309.0 56 19765.0 57 18033.5 58 16968.0 59 16006.0 60 14210.0 61 11812.5 62 9466.0 63 6886.0 64 4599.5 65 3338.0 66 2473.0 67 1848.0 68 1316.0 69 871.5 70 619.5 71 460.5 72 375.0 73 352.5 74 285.5 75 231.5 76 171.5 77 87.5 78 52.0 79 33.0 80 21.5 81 10.5 82 7.0 83 8.0 84 4.5 85 1.5 86 1.5 87 1.0 88 1.5 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019925761741156463 2 0.003649768239716778 3 9.864238485721022E-5 4 0.0 5 0.0025647020062874656 6 0.0 7 9.864238485721022E-5 8 0.0 9 9.864238485721022E-5 10-11 0.0 12-13 0.005967864283861218 14-15 0.002712665583573281 16-17 0.0 18-19 4.93211924286051E-4 20-21 0.0010357450410007072 22-23 0.0014796357728581533 24-25 4.43890731857446E-4 26-27 1.4796357728581532E-4 28-29 9.864238485721022E-5 30-31 0.0 32-33 9.864238485721022E-5 34-35 0.0 36-37 0.0016275993501439685 38-39 0.009814917293292416 40-41 2.9592715457163064E-4 42-43 0.0 44-45 9.37102656143497E-4 46-47 2.466059621430255E-4 48-49 0.0 50-51 0.0012330298107151277 52-53 4.43890731857446E-4 54-55 0.0 56-57 3.4524834700023577E-4 58-59 2.466059621430255E-4 60-61 0.0020714900820014144 62-63 4.932119242860511E-5 64-65 4.932119242860511E-5 66-67 9.864238485721022E-5 68-69 4.932119242860511E-5 70-71 5.425331167146562E-4 72-73 3.4524834700023577E-4 74-75 0.0030085927381449116 76-77 9.864238485721022E-5 78-79 9.864238485721022E-5 80-81 0.0015782781577153636 82-83 4.932119242860511E-5 84-85 0.00221945365928723 86-87 1.9728476971442045E-4 88-89 0.0020221688895728095 90-91 0.0012823510031437328 92-93 0.0 94-95 0.0 96-97 1.9728476971442045E-4 98-99 4.43890731857446E-4 100-101 0.0016769205425725738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1013763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.794611790306334 #Duplication Level Percentage of deduplicated Percentage of total 1 74.50425055923183 35.609017322061995 2 13.853242011635396 13.242206479665523 3 4.767649456608913 6.836038647926637 4 2.242505759002276 4.287187687561602 5 1.1799004233401642 2.8196441342380627 6 0.7275553639137651 2.086393570452807 7 0.5077751777769135 1.6988242249020957 8 0.34026210015356406 1.3010155987035144 9 0.26682502514589945 1.1477518643508289 >10 1.4201723689132064 12.507350459835601 >50 0.11491955323425646 3.804424497497539 >100 0.06640711713831539 5.594724262615076 >500 0.004996146676380137 1.625256888313903 >1k 0.00291441889455508 2.961778209447705 >5k 0.0 0.0 >10k+ 6.245183345475171E-4 4.478386152427126 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19432 1.9168188225453089 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14761 1.45606024287728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10818 1.0671133193853 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3715 0.36645645974453595 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3316 0.32709814818650906 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3120 0.3077642407544959 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3098 0.3055941082876372 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2875 0.28359685646447935 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2507 0.24729645883702603 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.17410380927297603 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1591 0.15694003430782147 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1554 0.15329026606810467 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1453 0.14332738519752644 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1346 0.13277265001780497 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1209 0.11925864329236716 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1137 0.11215639158264801 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1082 0.10673106041550146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.864238485721021E-5 2 0.0 9.864238485721021E-5 0.0 0.0 9.864238485721021E-5 3 0.0 9.864238485721021E-5 0.0 0.0 9.864238485721021E-5 4 0.0 9.864238485721021E-5 0.0 0.0 9.864238485721021E-5 5 0.0 9.864238485721021E-5 0.0 0.0 9.864238485721021E-5 6 0.0 9.864238485721021E-5 0.0 0.0 9.864238485721021E-5 7 0.0 9.864238485721021E-5 0.0 0.0 9.864238485721021E-5 8 0.0 9.864238485721021E-5 0.0 0.0 9.864238485721021E-5 9 0.0 9.864238485721021E-5 0.0 0.0 9.864238485721021E-5 10-11 0.0 9.864238485721021E-5 0.0 0.0 1.9728476971442042E-4 12-13 0.0 9.864238485721021E-5 0.0 1.9728476971442042E-4 1.9728476971442042E-4 14-15 0.0 9.864238485721021E-5 0.0 1.9728476971442042E-4 1.9728476971442042E-4 16-17 0.0 9.864238485721021E-5 0.0 2.9592715457163064E-4 1.9728476971442042E-4 18-19 0.0 9.864238485721021E-5 0.0 3.9456953942884084E-4 1.9728476971442042E-4 20-21 0.0 9.864238485721021E-5 0.0 4.93211924286051E-4 1.9728476971442042E-4 22-23 0.0 9.864238485721021E-5 0.0 9.86423848572102E-4 1.9728476971442042E-4 24-25 0.0 9.864238485721021E-5 0.0 0.0016769205425725736 1.9728476971442042E-4 26-27 0.0 9.864238485721021E-5 0.0 0.002367417236573045 1.9728476971442042E-4 28-29 0.0 9.864238485721021E-5 0.0 0.0050800828201463255 1.9728476971442042E-4 30-31 0.0 9.864238485721021E-5 0.0 0.010357450410007072 1.9728476971442042E-4 32-33 0.0 9.864238485721021E-5 0.0 0.01583210276958224 1.9728476971442042E-4 34-35 0.0 9.864238485721021E-5 0.0 0.020665579627585542 2.9592715457163064E-4 36-37 0.0 9.864238485721021E-5 0.0 0.025647020062874656 2.9592715457163064E-4 38-39 0.0 1.9728476971442042E-4 0.0 0.0357578645107387 2.9592715457163064E-4 40-41 0.0 1.9728476971442042E-4 0.0 0.05622615936860982 2.9592715457163064E-4 42-43 0.0 1.9728476971442042E-4 0.0 0.07408043102776488 2.9592715457163064E-4 44-45 0.0 1.9728476971442042E-4 0.0 0.09380890799920691 2.9592715457163064E-4 46-47 0.0 1.9728476971442042E-4 0.0 0.12858034866137352 2.9592715457163064E-4 48-49 0.0 1.9728476971442042E-4 0.0 0.1516133455255321 3.9456953942884084E-4 50-51 0.0 1.9728476971442042E-4 0.0 0.18155130932969538 3.9456953942884084E-4 52-53 0.0 1.9728476971442042E-4 0.0 0.21484311421900384 3.9456953942884084E-4 54-55 0.0 1.9728476971442042E-4 0.0 0.25612495228174637 3.9456953942884084E-4 56-57 0.0 1.9728476971442042E-4 0.0 0.36438496966253453 3.9456953942884084E-4 58-59 0.0 1.9728476971442042E-4 0.0 0.44384141066501737 3.9456953942884084E-4 60-61 0.0 1.9728476971442042E-4 0.0 0.5081069243994898 3.9456953942884084E-4 62-63 0.0 1.9728476971442042E-4 0.0 0.5863303355912575 3.9456953942884084E-4 64-65 0.0 1.9728476971442042E-4 0.0 0.6574514950733061 3.9456953942884084E-4 66-67 0.0 1.9728476971442042E-4 0.0 0.7763155688262444 3.9456953942884084E-4 68-69 0.0 2.9592715457163064E-4 0.0 0.9329103547870656 3.9456953942884084E-4 70-71 0.0 2.9592715457163064E-4 0.0 1.0048206533479718 3.9456953942884084E-4 72-73 0.0 2.9592715457163064E-4 0.0 1.0910834189056022 3.9456953942884084E-4 74-75 0.0 2.9592715457163064E-4 0.0 1.2560628075792861 3.9456953942884084E-4 76-77 0.0 2.9592715457163064E-4 0.0 1.4348028089405513 3.9456953942884084E-4 78-79 0.0 2.9592715457163064E-4 0.0 1.6030373963145232 3.9456953942884084E-4 80-81 0.0 3.9456953942884084E-4 0.0 1.7450331093164773 3.9456953942884084E-4 82-83 0.0 3.9456953942884084E-4 0.0 1.9732915878760617 4.93211924286051E-4 84-85 0.0 3.9456953942884084E-4 0.0 2.3741249187433358 4.93211924286051E-4 86-87 0.0 3.9456953942884084E-4 0.0 2.694860633106554 4.93211924286051E-4 88-89 0.0 4.438907318574459E-4 0.0 3.1140907687496977 4.93211924286051E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 70 2.0527482E-4 33.929737 2 GGTATCA 11870 0.0 31.619074 1 GTATCAA 17280 0.0 30.077818 1 ATCACGC 80 4.4936666E-4 29.687056 3 CGTAGCT 65 0.0058357334 29.238985 8 TCACGCA 110 9.476515E-5 25.9087 4 GTAAGGT 390 0.0 25.57654 4 TATCAAC 21490 0.0 24.181843 2 GAGTACT 12545 0.0 24.160248 12-13 ATCAACG 21610 0.0 24.068357 3 TCAACGC 21900 0.0 23.749643 4 CAACGCA 22030 0.0 23.566372 5 GTACATG 10815 0.0 23.54567 1 GTCGGGA 405 0.0 23.457594 2 AGTACTT 12655 0.0 23.368376 12-13 TACATGG 10900 0.0 23.097122 2 AACGCAG 22535 0.0 23.02286 6 CATGGGT 735 0.0 22.618706 4 GTACTTT 13575 0.0 22.28825 14-15 ACATGGG 10805 0.0 22.024195 3 >>END_MODULE