FastQCFastQC Report
Thu 26 May 2016
SRR936819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113325
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91960.4351436717021755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67350.3186921084073675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45530.21544248991518106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104000.035.0117151
GGTATCA63850.034.1719441
ATCAACG125000.028.7453733
TCAACGC125750.028.573934
TATCAAC127200.028.3650072
CAACGCA128800.027.8946515
AACGCAG131550.027.236696
ACGCAGA149550.023.736597
CGCAGAG150150.023.5484668
GTACATG84300.022.7245561
GCAGAGT153700.022.201179
GAGTACT79050.021.84840612-13
TACATGG86300.021.5374452
ACATGGG85800.020.939233
GTACTTT84450.020.33978814-15
AGTACTT85050.020.33500512-13
AGAGTAC126750.020.3003610-11
GTGTAGC12300.019.3233661
GTATAGG7150.019.2801271
TATCGCG757.718947E-419.00248390-91
Produced by FastQC (version 0.11.5)