##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936819_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2113325 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55482285024783 31.0 31.0 34.0 30.0 34.0 2 31.6589734186649 33.0 31.0 34.0 30.0 34.0 3 31.657626725657437 33.0 31.0 34.0 30.0 34.0 4 35.24605396708978 37.0 35.0 37.0 33.0 37.0 5 35.27035500928631 37.0 35.0 37.0 33.0 37.0 6 35.31846923686608 37.0 35.0 37.0 33.0 37.0 7 35.24917700779577 37.0 35.0 37.0 32.0 37.0 8 35.21896915997303 37.0 35.0 37.0 32.0 37.0 9 36.79862254977346 39.0 37.0 39.0 33.0 39.0 10-11 36.66144062082264 39.0 37.0 39.0 32.0 39.0 12-13 36.539147551843655 39.0 37.0 39.0 32.0 39.0 14-15 37.64507186542534 40.0 37.0 41.0 32.0 41.0 16-17 37.57053931600677 40.0 37.0 41.0 32.0 41.0 18-19 37.52623898359221 40.0 37.0 41.0 32.0 41.0 20-21 37.46330190576461 40.0 37.0 41.0 32.0 41.0 22-23 37.31737073095714 40.0 37.0 41.0 31.5 41.0 24-25 37.137339027362096 39.5 36.5 41.0 31.0 41.0 26-27 37.096984609560764 39.0 36.5 41.0 31.0 41.0 28-29 37.17682845752546 40.0 37.0 41.0 31.0 41.0 30-31 37.04032366058226 39.0 36.5 41.0 31.0 41.0 32-33 36.848604686927 39.0 36.0 41.0 30.0 41.0 34-35 36.6561260099606 39.0 36.0 41.0 30.0 41.0 36-37 36.501242591650595 39.0 35.0 41.0 30.0 41.0 38-39 36.335723800172715 39.0 35.0 41.0 30.0 41.0 40-41 36.134267800740545 39.0 35.0 40.5 29.5 41.0 42-43 35.925135509209426 39.0 35.0 40.0 29.0 41.0 44-45 35.672919214981135 39.0 35.0 40.0 27.5 41.0 46-47 35.33542072326784 38.0 35.0 40.0 26.5 41.0 48-49 35.00270568890256 38.0 34.0 40.0 25.0 41.0 50-51 34.361778003856486 37.5 33.5 39.5 24.0 40.5 52-53 34.672374812203515 38.0 34.0 40.0 25.0 40.5 54-55 34.97931387742065 39.0 34.5 40.0 25.5 41.0 56-57 34.861779423420444 38.5 34.0 40.0 25.0 41.0 58-59 34.616074669064155 38.0 34.0 40.0 24.0 41.0 60-61 34.3271394603291 38.0 34.0 40.0 23.0 41.0 62-63 34.011790898229094 37.5 34.0 40.0 22.5 41.0 64-65 33.70534820720902 37.0 33.0 40.0 22.0 41.0 66-67 33.32380301184153 36.5 33.0 39.5 21.5 41.0 68-69 32.88595033892089 36.0 32.0 39.0 20.0 41.0 70-71 32.526361066092534 35.5 32.0 39.0 19.5 40.5 72-73 32.13772018028462 35.0 32.0 38.0 18.0 40.0 74-75 31.69812925129831 35.0 31.0 37.0 17.0 39.5 76-77 31.25177599280754 35.0 31.0 37.0 13.0 39.0 78-79 30.81311393183727 35.0 31.0 36.0 9.0 39.0 80-81 30.382327138513954 34.5 30.0 36.0 7.0 37.5 82-83 29.9595447931577 34.0 30.0 35.5 4.5 37.0 84-85 29.588863047567223 34.0 29.5 35.0 2.0 37.0 86-87 29.24913418428306 34.0 29.0 35.0 2.0 36.0 88-89 28.862110181822484 34.0 29.0 35.0 2.0 36.0 90-91 28.508750902014597 34.0 29.0 35.0 2.0 36.0 92-93 28.12845255698958 34.0 28.5 35.0 2.0 35.0 94-95 27.712537352276627 34.0 27.0 35.0 2.0 35.0 96-97 27.247432127098293 34.0 26.5 35.0 2.0 35.0 98-99 26.62130670862267 33.5 25.5 35.0 2.0 35.0 100-101 24.385859723421625 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4670431640503878 1101 2 0.38838755350325727 1101 3 0.5039880056279777 1101 4 0.3758364314383513 1101 5 0.43333771045013947 1101 6 0.38972238075491106 1101 7 0.4561212123955727 1101 8 0.3664081386317406 1101 9 0.4045147185582678 1101 10-11 0.4288634385654646 1101 12-13 0.5642496430655655 1101 14-15 0.7718164669961922 1101 16-17 0.823582859629731 1101 18-19 0.9090141447610307 1101 20-21 0.7075872718021614 1101 22-23 0.7493206400715238 1101 24-25 0.7920708004750594 1101 26-27 0.8290343083527247 1101 28-29 0.8823504113748797 1101 30-31 0.7439950124480745 1101 32-33 0.8003880704458339 1101 34-35 0.6475925736396491 1101 36-37 0.6643345967199323 1101 38-39 0.7126885894001589 1101 40-41 0.6221333840203798 1101 42-43 0.7187796114432459 1101 44-45 0.698748892784856 1101 46-47 0.7376892063406117 1101 48-49 0.7891326256327034 1101 50-51 0.7860201416255777 1101 52-53 0.8956464236399739 1101 54-55 0.8300548501244265 1101 56-57 0.638927369775864 1101 58-59 0.6652914222492257 1101 60-61 0.7988213593999234 1101 62-63 0.8140668063467444 1101 64-65 0.7316932775799856 1101 66-67 0.4871204727609708 1101 68-69 0.5966953172786802 1101 70-71 0.6220009069452246 1101 72-73 0.5939268720313322 1101 74-75 0.5221542309673488 1101 76-77 0.5105295958511391 1101 78-79 0.49882503708966297 1101 80-81 0.5558264679702916 1101 82-83 0.49614414899534864 1101 84-85 0.6510038264926159 1101 86-87 0.7905971057020089 1101 88-89 0.8099948052455979 1101 90-91 0.7736565092711984 1101 92-93 0.8224442601201538 1101 94-95 1.0405451020408059 1101 96-97 0.8502093342807591 1101 98-99 0.9237108281286801 1101 100-101 1.2972047689258552 1102 1 0.468621825214413 1102 2 0.5040648491666815 1102 3 0.45605066076677403 1102 4 0.3383177142875837 1102 5 0.3402695046502515 1102 6 0.3429226366815925 1102 7 0.23398400130407992 1102 8 0.22661209834897278 1102 9 0.33191819054088256 1102 10-11 0.4505896260507356 1102 12-13 0.37019156378858753 1102 14-15 0.5587379660655003 1102 16-17 0.5044112491787232 1102 18-19 0.4523547472160061 1102 20-21 0.5626029099048679 1102 22-23 0.5034671100810968 1102 24-25 0.5483926472562715 1102 26-27 0.522036187604904 1102 28-29 0.6037271003848801 1102 30-31 0.5375585457994987 1102 32-33 0.6069276000633153 1102 34-35 0.43458877382075656 1102 36-37 0.3482756442550752 1102 38-39 0.41033567186951814 1102 40-41 0.35048976973716606 1102 42-43 0.33994660136808363 1102 44-45 0.2616865335473193 1102 46-47 0.22071117338056467 1102 48-49 0.3632187094520418 1102 50-51 0.11660382641144906 1102 52-53 0.38430413733930635 1102 54-55 0.4096817688766379 1102 56-57 0.40794114760873157 1102 58-59 0.44069566245852343 1102 60-61 0.5403317287851905 1102 62-63 0.5501024440251285 1102 64-65 0.558396970986081 1102 66-67 0.23345034795763553 1102 68-69 0.17366828083592623 1102 70-71 0.3366385283903597 1102 72-73 0.3398607978371615 1102 74-75 0.4026325637367911 1102 76-77 0.3725738381149988 1102 78-79 0.436711006791608 1102 80-81 0.3093829220235236 1102 82-83 0.45775045738699305 1102 84-85 0.5360359122392211 1102 86-87 0.5101143064983731 1102 88-89 0.5009107480707726 1102 90-91 0.3627249115941886 1102 92-93 0.5796385131592814 1102 94-95 0.44217568550226716 1102 96-97 0.436580791979722 1102 98-99 0.3870601624624932 1102 100-101 0.5829916820503591 1103 1 0.4308271829994901 1103 2 0.5399320017074416 1103 3 0.467942824274143 1103 4 0.4698826118092825 1103 5 0.35003257518000197 1103 6 0.29877637601763496 1103 7 0.2560793181302543 1103 8 0.19216022126012433 1103 9 0.40171102541764725 1103 10-11 0.4005670849778582 1103 12-13 0.21181413606438326 1103 14-15 0.338078692870738 1103 16-17 0.5854010707446093 1103 18-19 0.6159685928040872 1103 20-21 0.4730277255492794 1103 22-23 0.41402149890397055 1103 24-25 0.6272104211708225 1103 26-27 0.7437488633872888 1103 28-29 0.7205144757631388 1103 30-31 0.7865918123707303 1103 32-33 0.6857701944850874 1103 34-35 0.7120146744759168 1103 36-37 0.6547375873259469 1103 38-39 0.635380779342313 1103 40-41 0.5422522992811736 1103 42-43 0.6023828405243066 1103 44-45 0.6651222995942803 1103 46-47 0.6297244619762026 1103 48-49 0.6593635274873293 1103 50-51 0.5198191458311143 1103 52-53 0.5740338166887469 1103 54-55 0.5859563019218541 1103 56-57 0.5324402039229099 1103 58-59 0.6925549210649109 1103 60-61 0.6996286296662646 1103 62-63 0.5933146431524676 1103 64-65 0.594255917563224 1103 66-67 0.5490804798507725 1103 68-69 0.6250512896284661 1103 70-71 0.4436844118839076 1103 72-73 0.49326784301149473 1103 74-75 0.5279759225288707 1103 76-77 0.5559252549607265 1103 78-79 0.5065770874916886 1103 80-81 0.5791615737533107 1103 82-83 0.36790741416993455 1103 84-85 0.58984464439758 1103 86-87 0.7837071103748343 1103 88-89 0.6509448382776135 1103 90-91 0.5575481642558131 1103 92-93 0.5886178635103683 1103 94-95 0.6336631633031082 1103 96-97 0.7157819160954269 1103 98-99 0.6888145164353467 1103 100-101 0.9819310865810635 1104 1 0.47070365539408243 1104 2 0.4544737270381347 1104 3 0.4676334258628536 1104 4 0.46202018401672973 1104 5 0.46459110304613915 1104 6 0.52275301469939 1104 7 0.5116748521322094 1104 8 0.4065924404115364 1104 9 0.4802866339301559 1104 10-11 0.5794975746269202 1104 12-13 0.5599000955181381 1104 14-15 0.5944946541206164 1104 16-17 0.6883991645259258 1104 18-19 0.6565762651894076 1104 20-21 0.6516357803371378 1104 22-23 0.6685691920317325 1104 24-25 0.5501418262683657 1104 26-27 0.6548704036583288 1104 28-29 0.6593564334323361 1104 30-31 0.6325907810426941 1104 32-33 0.6057597073853813 1104 34-35 0.6414498195860929 1104 36-37 0.718398048642463 1104 38-39 0.6582212189893397 1104 40-41 0.5802813618735172 1104 42-43 0.7789928420886767 1104 44-45 0.76811430969898 1104 46-47 0.6246558902496488 1104 48-49 0.6099464016479317 1104 50-51 0.559996615369279 1104 52-53 0.784055080587045 1104 54-55 0.6003647657645246 1104 56-57 0.6478237942191569 1104 58-59 0.8299576546636018 1104 60-61 0.7492418816521536 1104 62-63 0.7042444027603239 1104 64-65 0.7711179571592695 1104 66-67 0.6172706729640467 1104 68-69 0.6445294839932458 1104 70-71 0.5509923560067875 1104 72-73 0.5833879521700425 1104 74-75 0.46482909733898126 1104 76-77 0.5077095104056184 1104 78-79 0.6106204148572232 1104 80-81 0.560655608853228 1104 82-83 0.6245116796963472 1104 84-85 0.7923957327962974 1104 86-87 0.8004105688001459 1104 88-89 0.6894904339854371 1104 90-91 0.7176705330656681 1104 92-93 0.5464038107967575 1104 94-95 0.45528860581816133 1104 96-97 0.5241043857204986 1104 98-99 0.761989847995725 1104 100-101 1.1274303246729893 1105 1 0.2572516206822044 1105 2 0.3022463027634643 1105 3 0.26560107855491566 1105 4 0.24829977069001075 1105 5 0.23341662147795716 1105 6 0.19960269902939132 1105 7 0.21146085861113306 1105 8 0.3794909294517623 1105 9 0.40413331525715535 1105 10-11 0.37788198265699435 1105 12-13 0.4214108188398811 1105 14-15 0.4309356226481995 1105 16-17 0.452562934595214 1105 18-19 0.5088941342395614 1105 20-21 0.5512793215161125 1105 22-23 0.4693985647115042 1105 24-25 0.5673765807078439 1105 26-27 0.5709459280015707 1105 28-29 0.5364159732871556 1105 30-31 0.3228978203654833 1105 32-33 0.48326437740973915 1105 34-35 0.4008608053750251 1105 36-37 0.4711521807272945 1105 38-39 0.5522920614853817 1105 40-41 0.511491903123428 1105 42-43 0.5256481588227402 1105 44-45 0.48274803791538545 1105 46-47 0.5948339423174716 1105 48-49 0.5994222948639845 1105 50-51 0.38106847463011206 1105 52-53 0.3909495735051678 1105 54-55 0.6325093118854568 1105 56-57 0.6383601967634362 1105 58-59 0.789270510826853 1105 60-61 0.6177600060757982 1105 62-63 0.5084509715338257 1105 64-65 0.6896484955717028 1105 66-67 0.7605192364882072 1105 68-69 0.7829401387424824 1105 70-71 0.7409116059654082 1105 72-73 0.7704738569724796 1105 74-75 0.7932464911106969 1105 76-77 0.5044978341624464 1105 78-79 0.5767414571593683 1105 80-81 0.7055173997780102 1105 82-83 0.8486693018854652 1105 84-85 0.7792605503697771 1105 86-87 0.8700802384698143 1105 88-89 0.9814964163457489 1105 90-91 0.9756243530273672 1105 92-93 0.8889087172780421 1105 94-95 1.0375218232876016 1105 96-97 1.019715153969063 1105 98-99 1.087387095637066 1105 100-101 1.3671840547810525 1106 1 0.6522055341332305 1106 2 0.521601469806491 1106 3 0.5182152534234241 1106 4 0.5167111931476782 1106 5 0.48253394689585605 1106 6 0.42011772272360304 1106 7 0.4545007718822589 1106 8 0.4469328064312066 1106 9 0.6525973336633655 1106 10-11 0.6707994008634373 1106 12-13 0.5948617000942278 1106 14-15 0.7887477306048183 1106 16-17 0.806963052001116 1106 18-19 0.8144234549422933 1106 20-21 0.7847065085560132 1106 22-23 0.8753892243148726 1106 24-25 1.019428935216844 1106 26-27 0.9417012077163847 1106 28-29 0.8399058399273116 1106 30-31 0.814424594117682 1106 32-33 0.8111421237845917 1106 34-35 0.831379474333005 1106 36-37 0.6546607944382217 1106 38-39 0.9193135496051354 1106 40-41 0.9111869081652486 1106 42-43 0.974829158003736 1106 44-45 1.0233490566956007 1106 46-47 0.9010105340431949 1106 48-49 1.1279783206743446 1106 50-51 0.9507517154116201 1106 52-53 1.145992464530714 1106 54-55 1.0212848583199587 1106 56-57 1.0452742442844212 1106 58-59 1.179038444814438 1106 60-61 1.2335586738975834 1106 62-63 1.3476517592895192 1106 64-65 1.1690126337508673 1106 66-67 1.2245536203202718 1106 68-69 1.201326115365454 1106 70-71 1.2462203171989756 1106 72-73 1.1558864108821467 1106 74-75 1.0808753180458872 1106 76-77 1.1256790653147704 1106 78-79 1.1711872823855423 1106 80-81 1.1708658538547887 1106 82-83 1.2207652812068766 1106 84-85 1.2188012471359073 1106 86-87 1.1190010983733316 1106 88-89 1.1239520222406618 1106 90-91 1.1837553032103365 1106 92-93 1.2652307171549566 1106 94-95 1.2598962865580141 1106 96-97 1.2069935385017807 1106 98-99 1.2722309647294914 1106 100-101 1.6171117412694294 1107 1 0.23338405563816877 1107 2 0.36043768742543847 1107 3 0.24798808741251221 1107 4 0.27786673260991535 1107 5 0.3135438074874912 1107 6 0.2273855238710354 1107 7 0.34932014647041854 1107 8 0.4155913155521489 1107 9 0.411343045996297 1107 10-11 0.5520984259910904 1107 12-13 0.48464295854260087 1107 14-15 0.4130191767905771 1107 16-17 0.5276380096702908 1107 18-19 0.4794476709695701 1107 20-21 0.48091631144722413 1107 22-23 0.6196212664047849 1107 24-25 0.5804137775685732 1107 26-27 0.5981054404551998 1107 28-29 0.45191361378479655 1107 30-31 0.5138427568986543 1107 32-33 0.457149607177314 1107 34-35 0.5823469133317261 1107 36-37 0.5862675061367426 1107 38-39 0.5563663037949169 1107 40-41 0.6945924406357236 1107 42-43 0.7839922171667979 1107 44-45 0.744418742233151 1107 46-47 0.753044253468957 1107 48-49 0.6975567738808124 1107 50-51 0.5775973564531114 1107 52-53 0.5376557334466199 1107 54-55 0.7326184546416528 1107 56-57 0.7255879707559174 1107 58-59 0.6766679923419829 1107 60-61 0.7836254513530037 1107 62-63 0.8399131059371356 1107 64-65 0.7265040486037222 1107 66-67 0.7748245081464162 1107 68-69 0.8175207277348377 1107 70-71 0.8611484394375353 1107 72-73 0.8170241888169301 1107 74-75 0.9217333813622979 1107 76-77 0.811498742633944 1107 78-79 0.9012973155387556 1107 80-81 1.0256728493452236 1107 82-83 0.9542425221645665 1107 84-85 0.9506020599465792 1107 86-87 1.019831670574188 1107 88-89 0.9743592371497449 1107 90-91 1.0001499290235287 1107 92-93 1.1812251934180473 1107 94-95 1.047317543377435 1107 96-97 0.9331800894928186 1107 98-99 0.7707185363214499 1107 100-101 1.1716361959978983 1108 1 -0.020668672492323736 1108 2 0.08964132490424959 1108 3 0.040811034273346536 1108 4 0.12241032191921164 1108 5 0.07162092747886817 1108 6 0.10487831362704014 1108 7 -0.06787186394193157 1108 8 -0.03945089372734856 1108 9 -0.057250102058468144 1108 10-11 -0.04733942519153089 1108 12-13 -0.14315629064248725 1108 14-15 0.06086767334296184 1108 16-17 0.08052140153672838 1108 18-19 0.14202514660786392 1108 20-21 0.26800324883326 1108 22-23 0.2703796305230739 1108 24-25 0.3602266341511182 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-0.9347158209866535 2316 70-71 -1.5448502645525615 2316 72-73 -1.2602037163880198 2316 74-75 -1.8137929208067582 2316 76-77 -2.113936176344737 2316 78-79 -2.5490016362666523 2316 80-81 -2.3161072181880726 2316 82-83 -2.2494819535828654 2316 84-85 -1.8751710430862047 2316 86-87 -1.8923955410791358 2316 88-89 -2.3112798965525165 2316 90-91 -2.384937261802545 2316 92-93 -2.7217397915411965 2316 94-95 -2.681210822884072 2316 96-97 -2.907452201856831 2316 98-99 -3.0404704181960547 2316 100-101 -2.7376785128863936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6382.0 3 2023.0 4 2030.0 5 2233.0 6 2499.0 7 2924.0 8 3517.0 9 4436.0 10 5414.0 11 6614.0 12 8310.0 13 9965.0 14 11063.0 15 12846.0 16 15827.0 17 19843.0 18 17809.0 19 13035.0 20 12731.0 21 13466.0 22 14860.0 23 16611.0 24 18598.0 25 21794.0 26 25221.0 27 29480.0 28 34842.0 29 42357.0 30 50854.0 31 62803.0 32 79582.0 33 102164.0 34 136418.0 35 191676.0 36 280368.0 37 404912.0 38 363230.0 39 64545.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30097326411839 19.363391708979066 14.714251624474212 22.62138340242833 2 14.96455224234065 20.507929801693383 40.825879704974625 23.701638250991344 3 18.91214661029509 25.32317458032642 30.041682075097814 25.722996734280677 4 12.596716495700171 17.20878444007486 38.133661841700025 32.06083722252494 5 13.600563127555876 37.4495930257069 34.41103479899027 14.538809047746945 6 30.665161015897436 36.943090000843135 18.043130831515217 14.348618151744208 7 27.50638216433179 33.17394305247704 21.418538760308696 17.90113602288247 8 25.614099735613376 35.150016435356 20.24490101368729 18.99098281534333 9 25.99986829968738 17.204750875593895 20.99265083831039 35.80272998640834 10-11 24.236148398754445 27.165265651316407 27.842376358253805 20.756209591675336 12-13 25.422832943423117 24.645285899032555 28.205558109173438 21.72632304837089 14-15 22.549844312012556 25.824715314071028 26.75763543718153 24.86780493673488 16-17 21.60133890991397 29.168093735063156 27.194381163928828 22.03618619109405 18-19 21.48156339860242 27.763607606851913 29.722319641325097 21.032509353220565 20-21 22.293873747255848 26.812536817561057 29.840952871627703 21.052636563555396 22-23 22.013677574063184 26.774428940920874 29.680368864530266 21.531524620485676 24-25 21.860496498562966 26.957371957253585 29.7644401725387 21.41769137164475 26-27 21.658590511721272 27.16871391473758 29.678342569424554 21.49435300411659 28-29 21.474616366591032 27.20423483554239 29.77687990787495 21.544268889991628 30-31 21.70863682726843 26.941526775994312 29.73978997521392 21.61004642152334 32-33 21.45441694172542 27.168348265094146 29.69294732317178 21.684287470008652 34-35 21.891094269487336 27.163209473389706 29.473588533009774 21.472107724113183 36-37 21.899161728638497 26.94419990687715 29.440597728455753 21.716040636028602 38-39 21.595631788787415 27.28221732120636 29.566547307966985 21.555603582039243 40-41 21.882165379655476 27.07451391129791 29.5460630999683 21.497257609078314 42-43 21.69483789146331 27.149039853541357 29.274754610035863 21.88136764495947 44-45 21.823002167112673 27.309146730453083 29.13205421214033 21.735796890293916 46-47 22.015825029543826 27.43383023134005 28.704433692245757 21.845911046870366 48-49 22.012338642332754 27.581462307114034 28.535856102193346 21.870342948359866 50-51 21.916599317714688 27.724946485686324 28.459296581118704 21.89915761548028 52-53 22.087279826398486 27.886352361079027 28.133414503906412 21.89295330861607 54-55 21.962990797999822 27.7170873154422 28.280261561677072 22.039660324880906 56-57 21.99033350313695 28.00725200308298 28.129728383652647 21.87268611012743 58-59 22.155675014369464 27.69054569867782 28.205408031180074 21.948371255772642 60-61 22.106004226458044 27.69300584398845 28.504280312597867 21.69670961695564 62-63 21.954804092276262 27.749686024448074 28.549959695127075 21.74555018814859 64-65 21.904122613533 27.758628157665584 28.375279325013956 21.96196990378746 66-67 21.92173821226982 27.970826193451302 28.06988095908877 22.037554635190105 68-69 21.748726964815678 27.84589734806727 28.322680419936496 22.08269526718056 70-71 21.966671218291758 27.68766164875638 28.2865829372749 22.059084195676963 72-73 21.950453025788207 27.65904177378622 28.284507956821514 22.105997243604065 74-75 21.832719389693082 28.03743537617015 28.01199408815466 22.11785114598211 76-77 21.980464386809594 27.885671193195794 28.044661080757255 22.089203339237365 78-79 21.873911718742786 27.910639664941357 28.020394050441087 22.195054565874766 80-81 21.862062142426375 28.065298873089823 27.95479010197387 22.117848882509932 82-83 21.96997781833763 28.01231472867116 27.97486393905606 22.04284351393515 84-85 21.92003196959858 28.02401413063833 27.874440727336776 22.181513172426314 86-87 21.945145481663868 27.974407538057243 27.928644144037502 22.151802836241387 88-89 21.975743479612696 27.855381561738756 27.885668468015346 22.2832064906332 90-91 21.974510606533737 27.81449036009634 28.06037377783323 22.150625255536696 92-93 22.020223018612032 27.7773857915134 28.022111497941044 22.18027969193352 94-95 21.85591919601398 27.76481486129574 27.971082303811507 22.40818363887877 96-97 21.8475208383704 27.712196878939128 28.05038008609807 22.389902196592402 98-99 21.68341579983665 27.793548851691053 27.890646763529276 22.632388584943026 100-101 21.754892704208313 27.814237602251605 27.92461983270223 22.506249860837855 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 547.0 1 402.0 2 316.5 3 386.0 4 375.5 5 400.0 6 688.0 7 1385.0 8 2331.0 9 2838.0 10 2558.0 11 2262.0 12 2278.0 13 2405.0 14 2637.0 15 2825.5 16 3016.0 17 3168.0 18 3304.0 19 3670.0 20 4188.0 21 4738.0 22 5664.0 23 7063.0 24 8651.5 25 10621.0 26 13514.0 27 16936.5 28 20045.5 29 23282.5 30 27368.0 31 31584.5 32 35863.0 33 40766.5 34 46390.0 35 50958.0 36 56486.5 37 62337.0 38 64606.5 39 65926.5 40 67057.5 41 68942.0 42 71114.0 43 73638.0 44 76407.0 45 78224.5 46 78662.0 47 79361.0 48 83108.5 49 82493.5 50 77517.0 51 73587.0 52 69282.0 53 66423.5 54 63807.0 55 58718.5 56 52856.0 57 47197.5 58 42028.5 59 36972.0 60 31211.5 61 24997.5 62 19523.5 63 14981.0 64 11136.0 65 8340.0 66 6231.5 67 4676.5 68 3312.0 69 2207.0 70 1513.5 71 1017.5 72 713.5 73 516.5 74 353.0 75 242.0 76 148.0 77 76.0 78 52.0 79 38.5 80 26.0 81 19.0 82 15.5 83 12.5 84 11.5 85 7.0 86 5.5 87 6.0 88 6.5 89 4.0 90 1.5 91 2.0 92 1.5 93 1.0 94 1.0 95 3.5 96 3.5 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05947972980966013 2 0.09695621828161784 3 0.06558385482592596 4 0.13130966604757904 5 0.10684584718394 6 0.10253983651355092 7 0.11186163983296464 8 0.09572592951864951 9 0.11697207007914069 10-11 0.1086676209291046 12-13 0.09186944743472963 14-15 0.09705085587876923 16-17 0.1113884518472076 18-19 0.08926691351306591 20-21 0.0738646445766742 22-23 0.07320218139661434 24-25 0.06317059609856505 26-27 0.05394343037630274 28-29 0.06686146238746998 30-31 0.04970839790377722 32-33 0.04963741970591366 34-35 0.04570995942413022 36-37 0.05022890468810996 38-39 0.050370861083837086 40-41 0.06579678941951664 42-43 0.06291034270639868 44-45 0.05051281747956419 46-47 0.037168916281215617 48-49 0.044337714265434806 50-51 0.02709001218459063 52-53 0.04263423751670945 54-55 0.04791028355790047 56-57 0.051151621260336205 58-59 0.05711378988087492 60-61 0.05462955295565046 62-63 0.061396141151976155 64-65 0.04100173896584766 66-67 0.06444820366010907 68-69 0.054345640164196234 70-71 0.024913347450108245 72-73 0.01992121420037145 74-75 0.02921935812049732 76-77 0.043840866880389906 78-79 0.06324157429642861 80-81 0.03894337122780452 82-83 0.057728934262359075 84-85 0.06238983592206594 86-87 0.06506334804159322 88-89 0.08761075556291625 90-91 0.06380939987933706 92-93 0.07443247015958264 94-95 0.08261862231317946 96-97 0.08969278270024725 98-99 0.12156199354098399 100-101 0.11725598287059492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2113325.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.181474119485735 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34418432530755 49.959198182753035 2 11.853696066741145 14.741605903886287 3 3.4456603863179107 6.427687264090931 4 1.4845393079803604 3.692433702341601 5 0.7331570119737756 2.279439188278341 6 0.45665982876861605 1.7037468794390689 7 0.29359076663716455 1.2779134660158178 8 0.23210057330113076 1.154588463347365 9 0.1568346225105434 0.8776987218610803 >10 0.8989382453793672 10.14018004218897 >50 0.07001395603268704 3.0143391205776897 >100 0.029480204183069264 3.252867084570657 >500 6.105092622183414E-4 0.2340379857015897 >1k 3.815682888864367E-4 0.48829093123868916 >5k 1.5262731555457465E-4 0.7559730637088006 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9196 0.4351436717021755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6735 0.3186921084073675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4553 0.21544248991518106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.7318798575704164E-5 8 0.0 0.0 0.0 0.0 4.7318798575704164E-5 9 0.0 0.0 0.0 4.7318798575704164E-5 4.7318798575704164E-5 10-11 0.0 0.0 0.0 4.7318798575704164E-5 4.7318798575704164E-5 12-13 0.0 0.0 0.0 4.7318798575704164E-5 4.7318798575704164E-5 14-15 0.0 0.0 0.0 9.463759715140833E-5 4.7318798575704164E-5 16-17 0.0 0.0 0.0 9.463759715140833E-5 4.7318798575704164E-5 18-19 0.0 0.0 0.0 9.463759715140833E-5 4.7318798575704164E-5 20-21 0.0 0.0 0.0 1.182969964392604E-4 4.7318798575704164E-5 22-23 0.0 0.0 0.0 1.8927519430281665E-4 4.7318798575704164E-5 24-25 0.0 0.0 0.0 3.0757219074207707E-4 4.7318798575704164E-5 26-27 0.0 0.0 0.0 5.441661836205979E-4 4.7318798575704164E-5 28-29 0.0 0.0 0.0 8.990571729383791E-4 4.7318798575704164E-5 30-31 0.0 0.0 0.0 0.0022003241337702436 4.7318798575704164E-5 32-33 0.0 0.0 0.0 0.0032886565010114394 4.7318798575704164E-5 34-35 0.0 0.0 0.0 0.004660901659706861 4.7318798575704164E-5 36-37 0.0 0.0 0.0 0.007192457383507033 4.7318798575704164E-5 38-39 0.0 0.0 0.0 0.01102528006813907 4.7318798575704164E-5 40-41 0.0 0.0 0.0 0.014787124554907551 4.7318798575704164E-5 42-43 0.0 0.0 0.0 0.019045816426720926 4.7318798575704164E-5 44-45 0.0 0.0 0.0 0.020914908970461242 4.7318798575704164E-5 46-47 0.0 0.0 0.0 0.023115233104231483 4.7318798575704164E-5 48-49 0.0 0.0 0.0 0.02716099038245419 4.7318798575704164E-5 50-51 0.0 0.0 0.0 0.03141968225426756 4.7318798575704164E-5 52-53 0.0 0.0 0.0 0.04154590514946825 4.7318798575704164E-5 54-55 0.0 0.0 0.0 0.04880934073083884 4.7318798575704164E-5 56-57 0.0 0.0 0.0 0.06584410821809233 4.7318798575704164E-5 58-59 0.0 0.0 0.0 0.09087575266463985 4.7318798575704164E-5 60-61 0.0 0.0 0.0 0.11356511658169 4.7318798575704164E-5 62-63 0.0 0.0 0.0 0.12414086806335986 4.7318798575704164E-5 64-65 0.0 0.0 0.0 0.1450557770338211 4.7318798575704164E-5 66-67 0.0 0.0 0.0 0.1968462020749293 4.7318798575704164E-5 68-69 0.0 0.0 0.0 0.27239066400104106 4.7318798575704164E-5 70-71 0.0 0.0 0.0 0.3363183608768173 4.7318798575704164E-5 72-73 0.0 0.0 0.0 0.4099937302591887 4.7318798575704164E-5 74-75 0.0 0.0 0.0 0.49795937681142277 4.7318798575704164E-5 76-77 0.0 0.0 0.0 0.5512403440076656 4.7318798575704164E-5 78-79 0.0 0.0 0.0 0.6211775283025565 4.7318798575704164E-5 80-81 0.0 0.0 0.0 0.7222978008588362 4.7318798575704164E-5 82-83 0.0 0.0 0.0 0.8129842783291732 4.7318798575704164E-5 84-85 0.0 0.0 0.0 0.9366519584067761 4.7318798575704164E-5 86-87 0.0 0.0 0.0 1.1299729099878153 4.7318798575704164E-5 88-89 0.0 0.0 0.0 1.374611098624206 4.7318798575704164E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10400 0.0 35.011715 1 GGTATCA 6385 0.0 34.171944 1 ATCAACG 12500 0.0 28.745373 3 TCAACGC 12575 0.0 28.57393 4 TATCAAC 12720 0.0 28.365007 2 CAACGCA 12880 0.0 27.894651 5 AACGCAG 13155 0.0 27.23669 6 ACGCAGA 14955 0.0 23.73659 7 CGCAGAG 15015 0.0 23.548466 8 GTACATG 8430 0.0 22.724556 1 GCAGAGT 15370 0.0 22.20117 9 GAGTACT 7905 0.0 21.848406 12-13 TACATGG 8630 0.0 21.537445 2 ACATGGG 8580 0.0 20.93923 3 GTACTTT 8445 0.0 20.339788 14-15 AGTACTT 8505 0.0 20.335005 12-13 AGAGTAC 12675 0.0 20.30036 10-11 GTGTAGC 1230 0.0 19.323366 1 GTATAGG 715 0.0 19.280127 1 TATCGCG 75 7.718947E-4 19.002483 90-91 >>END_MODULE