FastQCFastQC Report
Thu 26 May 2016
SRR936819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113325
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105060.4971312978363479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84090.3979037772230963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57520.27217772940745033No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.10968497509848225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71450.038.6979061
GTATCAA110450.033.42121
ATCAACG135100.027.1403623
TCAACGC135350.027.1253224
TATCAAC137900.026.6938782
CAACGCA137800.026.6775195
AACGCAG140050.026.2489286
ACGCAGA158200.022.9672327
GAGTACT93300.022.7296912-13
CGCAGAG159800.022.6183838
GTACATG81300.022.3807681
GTGTACG3200.022.2693751
GCAGAGT166900.021.400079
TACATGG83600.021.2490542
GTACTTT100350.021.1099214-15
ACATGGG80300.020.701713
AGAGTAC136450.020.53677210-11
AGTACTT98450.020.21398712-13
TATACCG1507.7287614E-418.9982555
CAGAGTA163350.018.84124410-11
Produced by FastQC (version 0.11.5)