##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936819_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2113325 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14561508523298 33.0 31.0 34.0 31.0 34.0 2 32.149958004566265 33.0 31.0 34.0 30.0 34.0 3 32.32414228762732 33.0 31.0 34.0 31.0 34.0 4 36.05368601611205 37.0 35.0 37.0 35.0 37.0 5 35.98314882945122 37.0 35.0 37.0 35.0 37.0 6 36.02475482947488 37.0 35.0 37.0 35.0 37.0 7 35.96323423988265 37.0 35.0 37.0 35.0 37.0 8 35.945515715755974 37.0 35.0 37.0 35.0 37.0 9 37.634982314599036 39.0 37.0 39.0 35.0 39.0 10-11 37.56598937692972 39.0 37.0 39.0 35.0 39.0 12-13 37.46931849100351 39.0 37.0 39.0 35.0 39.0 14-15 38.68567920220506 40.0 38.0 41.0 34.5 41.0 16-17 38.601559391007065 40.0 38.0 41.0 34.0 41.0 18-19 38.67074326002863 40.0 38.0 41.0 34.5 41.0 20-21 38.75868288124165 40.0 38.0 41.0 34.0 41.0 22-23 38.6927507600582 40.0 38.0 41.0 34.0 41.0 24-25 38.60099416795808 40.0 38.0 41.0 34.0 41.0 26-27 38.44786249157134 40.0 38.0 41.0 34.0 41.0 28-29 38.28201554422533 40.0 38.0 41.0 34.0 41.0 30-31 38.140480285805545 40.0 38.0 41.0 34.0 41.0 32-33 38.110358085008215 40.0 37.0 41.0 34.0 41.0 34-35 38.0225713035146 40.0 37.0 41.0 33.0 41.0 36-37 37.867959021920434 40.0 37.0 41.0 33.0 41.0 38-39 37.71653058568843 40.0 37.0 41.0 33.0 41.0 40-41 37.54423976435238 40.0 37.0 41.0 33.0 41.0 42-43 37.33213419611276 40.0 37.0 41.0 32.0 41.0 44-45 36.99807862018383 40.0 36.0 41.0 31.0 41.0 46-47 36.722381318538325 39.5 36.0 41.0 31.0 41.0 48-49 36.39773484911218 39.0 36.0 41.0 30.0 41.0 50-51 36.13770622123904 39.0 35.5 41.0 30.0 41.0 52-53 35.8926379047236 39.0 35.0 40.0 29.0 41.0 54-55 35.669568097666 39.0 35.0 40.0 28.0 41.0 56-57 35.40993718429489 38.5 35.0 40.0 28.0 41.0 58-59 35.08994215276874 38.0 34.0 40.0 27.0 41.0 60-61 34.77263908769356 38.0 34.0 40.0 26.5 41.0 62-63 34.41436835318751 37.5 34.0 40.0 26.0 41.0 64-65 34.0287210911715 37.0 33.5 39.5 26.0 41.0 66-67 33.65715472064164 36.0 33.0 39.0 25.0 40.5 68-69 33.22047650030166 36.0 32.5 39.0 24.5 40.0 70-71 32.75163616575775 35.0 32.0 38.0 23.0 40.0 72-73 32.41059420581311 35.0 32.0 38.0 22.0 40.0 74-75 31.97187441590858 35.0 31.0 37.0 21.5 39.0 76-77 30.570751304224387 33.5 29.5 35.5 20.0 38.5 78-79 31.28399063087788 34.0 31.0 36.0 20.5 38.5 80-81 31.112997764186765 34.0 31.0 36.0 20.0 37.5 82-83 30.768836785634015 34.0 31.0 35.5 18.5 37.0 84-85 30.380833993824893 34.0 30.5 35.0 17.5 37.0 86-87 30.026186932913774 34.0 30.0 35.0 12.5 36.0 88-89 29.669420226420453 34.0 30.0 35.0 7.0 36.0 90-91 29.242821619959066 34.0 29.0 35.0 4.0 35.5 92-93 28.786368400506312 34.0 29.0 35.0 2.0 35.0 94-95 28.315088781895827 34.0 29.0 35.0 2.0 35.0 96-97 27.69560100789041 33.0 27.5 35.0 2.0 35.0 98-99 26.96789253900843 33.0 26.5 35.0 2.0 35.0 100-101 23.902174062200558 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24866868017556953 1101 2 0.17491410775785 1101 3 0.23640036481734938 1101 4 0.192896821752214 1101 5 0.22306120656688933 1101 6 0.13959523910515514 1101 7 0.2524402237311918 1101 8 0.21858787093812282 1101 9 0.2027276582879125 1101 10-11 0.2877128273097753 1101 12-13 0.30762764276799714 1101 14-15 0.4008343417979674 1101 16-17 0.4839846232246501 1101 18-19 0.4283650756920636 1101 20-21 0.407963807803867 1101 22-23 0.24134799230728987 1101 24-25 0.34587413447611226 1101 26-27 0.3213871899193208 1101 28-29 0.2895126332235165 1101 30-31 0.34478894259460446 1101 32-33 0.4403298441835304 1101 34-35 0.44403288623135495 1101 36-37 0.4725012151594896 1101 38-39 0.49468721309991537 1101 40-41 0.38824286443269074 1101 42-43 0.5231599038607655 1101 44-45 0.5274811292448902 1101 46-47 0.41396629872958357 1101 48-49 0.18971830756488117 1101 50-51 0.3665718979395507 1101 52-53 0.2725551388594667 1101 54-55 0.25319817852647475 1101 56-57 0.34115187790929014 1101 58-59 0.4945345058700781 1101 60-61 0.5043064524538607 1101 62-63 0.5213420762458512 1101 64-65 0.5377919472362294 1101 66-67 0.3955054267950615 1101 68-69 0.2762519672283119 1101 70-71 0.264792627473291 1101 72-73 0.42144310157551246 1101 74-75 0.3936974835076157 1101 76-77 0.3284389092997557 1101 78-79 0.45164635052224966 1101 80-81 0.42324935706347233 1101 82-83 0.5521204728608353 1101 84-85 0.4767207435099827 1101 86-87 0.43926122391263434 1101 88-89 0.39026735176358684 1101 90-91 0.5121206738451711 1101 92-93 0.7843402309865581 1101 94-95 0.6387265628088414 1101 96-97 0.8083885135026385 1101 98-99 0.8949263600673305 1101 100-101 0.8180797923404022 1102 1 0.28130938122708216 1102 2 0.197996193001984 1102 3 0.2725588294359298 1102 4 0.1343965833562919 1102 5 0.19390834397338352 1102 6 0.2050244801798229 1102 7 0.24527628661412137 1102 8 0.3123756192858167 1102 9 0.4064347986645913 1102 10-11 0.3980483793442886 1102 12-13 0.3402184314174477 1102 14-15 0.5067753692341554 1102 16-17 0.4894366877780243 1102 18-19 0.4328953798897288 1102 20-21 0.37336893013392114 1102 22-23 0.4006346581029945 1102 24-25 0.47516646147715846 1102 26-27 0.41784385601651763 1102 28-29 0.33251747148834454 1102 30-31 0.40343646165790403 1102 32-33 0.5177045634960606 1102 34-35 0.47746113129120005 1102 36-37 0.6013144054329302 1102 38-39 0.5512704579420173 1102 40-41 0.48142239610472615 1102 42-43 0.5636733651351093 1102 44-45 0.5079689531869818 1102 46-47 0.5550905448792349 1102 48-49 0.44318230643040124 1102 50-51 0.6246010391894075 1102 52-53 0.6298237580278396 1102 54-55 0.5820520133510385 1102 56-57 0.5323528908965685 1102 58-59 0.603595002604564 1102 60-61 0.4979418953957193 1102 62-63 0.6747734701418011 1102 64-65 0.6372980712758078 1102 66-67 0.6150206176960467 1102 68-69 0.5665700778373264 1102 70-71 0.40546732702184585 1102 72-73 0.4093678734244648 1102 74-75 0.3031994872616508 1102 76-77 0.29240483487332725 1102 78-79 0.4607070705784331 1102 80-81 0.3892889363700398 1102 82-83 0.4733368168834602 1102 84-85 0.3339882460698078 1102 86-87 0.3107597907455606 1102 88-89 0.5411909545233975 1102 90-91 0.6080471742041631 1102 92-93 0.6837071139403008 1102 94-95 0.8597448204751998 1102 96-97 0.8266091084540044 1102 98-99 1.0538290241836634 1102 100-101 1.1152690653054158 1103 1 0.04921008298892815 1103 2 -0.18987215092312226 1103 3 9.8682007910611E-4 1103 4 0.1162496520321028 1103 5 0.17925269558575962 1103 6 0.17495722167069516 1103 7 0.25550172773531443 1103 8 0.22125943601119502 1103 9 0.16888998245284625 1103 10-11 0.21170857343052774 1103 12-13 0.29045260530484995 1103 14-15 0.3013419069354839 1103 16-17 0.38443256959999417 1103 18-19 0.33783962492587705 1103 20-21 0.3489476785117205 1103 22-23 0.3716762177628894 1103 24-25 0.41183182126814444 1103 26-27 0.339145524461145 1103 28-29 0.46556683795756015 1103 30-31 0.34893164090755846 1103 32-33 0.4802212413572775 1103 34-35 0.4736327959975526 1103 36-37 0.3580122446304941 1103 38-39 0.3575866185236123 1103 40-41 0.3864591355208091 1103 42-43 0.39519064780623836 1103 44-45 0.4490663437918627 1103 46-47 0.29075204178883496 1103 48-49 0.24244062915170161 1103 50-51 0.20902206793358857 1103 52-53 0.2442974569013927 1103 54-55 0.19282370824723216 1103 56-57 0.13589898156537572 1103 58-59 0.31455513073916563 1103 60-61 0.2413877112154026 1103 62-63 0.3165306810056876 1103 64-65 0.2753872164068838 1103 66-67 0.23770423119718487 1103 68-69 0.3428960754444219 1103 70-71 0.2334686075574055 1103 72-73 0.30860573189084306 1103 74-75 0.28286298196880466 1103 76-77 0.3394877161188532 1103 78-79 0.3489941212639387 1103 80-81 0.39414251057372596 1103 82-83 0.4320869574485755 1103 84-85 0.43599951761932587 1103 86-87 0.20884759860849655 1103 88-89 0.5117945588662778 1103 90-91 0.6735570026184767 1103 92-93 0.845546141300602 1103 94-95 0.7259826495621979 1103 96-97 1.0846811932854301 1103 98-99 1.288158824900318 1103 100-101 1.2918668727935092 1104 1 0.2538956727868964 1104 2 0.09356460491286356 1104 3 0.17474995068116073 1104 4 0.16587738896233617 1104 5 0.1563294020570467 1104 6 0.20775113567647452 1104 7 0.25612689793341303 1104 8 0.2888224310341698 1104 9 0.30110623687799887 1104 10-11 0.33818763109798766 1104 12-13 0.3401116297148832 1104 14-15 0.37328167274291246 1104 16-17 0.3005708254968056 1104 18-19 0.3130570439405602 1104 20-21 0.28349369138593516 1104 22-23 0.30524246900134955 1104 24-25 0.341951814547123 1104 26-27 0.3093552085155338 1104 28-29 0.3392453764846195 1104 30-31 0.4523005492642085 1104 32-33 0.447739912450551 1104 34-35 0.411857984736379 1104 36-37 0.31922069456762614 1104 38-39 0.34228733060347594 1104 40-41 0.486408148516702 1104 42-43 0.40632464512941624 1104 44-45 0.5245850496489908 1104 46-47 0.6496232011058396 1104 48-49 0.6376000504492083 1104 50-51 0.5575669104243062 1104 52-53 0.5562148000149634 1104 54-55 0.4748197915182786 1104 56-57 0.5558117242606144 1104 58-59 0.5019410618024125 1104 60-61 0.5062040710909486 1104 62-63 0.6075242323084566 1104 64-65 0.4987580947159742 1104 66-67 0.5769526364048119 1104 68-69 0.48453884195291863 1104 70-71 0.5539685698965542 1104 72-73 0.5956625650184932 1104 74-75 0.60074290757413 1104 76-77 0.4946585515525008 1104 78-79 0.5077670726582575 1104 80-81 0.5581926168932192 1104 82-83 0.6572108037227622 1104 84-85 0.8606415946444663 1104 86-87 0.7130470736741117 1104 88-89 0.8618924770900769 1104 90-91 0.9994647740808169 1104 92-93 0.9493203384001383 1104 94-95 0.8637915410004382 1104 96-97 0.939221064543247 1104 98-99 1.1197026882087933 1104 100-101 1.1802366212769329 1105 1 0.20270629443216137 1105 2 0.048863604700052576 1105 3 0.13725852224484925 1105 4 0.12913671127677873 1105 5 0.10235692564362608 1105 6 0.07434568297968269 1105 7 0.1572110529545725 1105 8 0.12693928804866061 1105 9 0.12605244292656437 1105 10-11 0.18449062700727836 1105 12-13 0.258354281815798 1105 14-15 0.30563311867702936 1105 16-17 0.2210876021159507 1105 18-19 0.19879809995720166 1105 20-21 0.24154209411938155 1105 22-23 0.2811043545089049 1105 24-25 0.3100419519286035 1105 26-27 0.26575491530894624 1105 28-29 0.1861950820957432 1105 30-31 0.28332547182456125 1105 32-33 0.2999306859011668 1105 34-35 0.29993641749985045 1105 36-37 0.13031998283226898 1105 38-39 0.2668924475314256 1105 40-41 0.10664886167079857 1105 42-43 0.10678750956865457 1105 44-45 0.3200421208934685 1105 46-47 0.4280305454580642 1105 48-49 0.34861716993088976 1105 50-51 0.26866797211591376 1105 52-53 0.40928164638889797 1105 54-55 0.3249250148518499 1105 56-57 0.394791649067308 1105 58-59 0.42038115543935817 1105 60-61 0.3541256146817844 1105 62-63 0.27047734289936187 1105 64-65 0.5493326850543383 1105 66-67 0.503738477363882 1105 68-69 0.3494320816493044 1105 70-71 0.20782872926852036 1105 72-73 0.39269975969347826 1105 74-75 0.3875539311961802 1105 76-77 0.31752452776493456 1105 78-79 0.26879305927479535 1105 80-81 0.47047596323168506 1105 82-83 0.4939597574008481 1105 84-85 0.44771272088881986 1105 86-87 0.4052439950048914 1105 88-89 0.44124387573281254 1105 90-91 0.7871749251767959 1105 92-93 0.6554292031704207 1105 94-95 0.7717376761119255 1105 96-97 1.002430730902521 1105 98-99 0.8804922178959593 1105 100-101 0.9162105873690116 1106 1 0.31325743937637185 1106 2 0.2046931862521717 1106 3 0.17430836767619695 1106 4 0.19255672614188057 1106 5 0.1270483801038722 1106 6 0.20308290680361551 1106 7 0.2569693767013419 1106 8 0.18458700407788342 1106 9 0.418916720761942 1106 10-11 0.32976859020432414 1106 12-13 0.399467649518769 1106 14-15 0.5087347883971987 1106 16-17 0.5625542526866454 1106 18-19 0.48452227657733005 1106 20-21 0.3881907001610472 1106 22-23 0.3769699389583039 1106 24-25 0.45189242036865807 1106 26-27 0.4792736363421142 1106 28-29 0.43711681307527783 1106 30-31 0.5908158970895556 1106 32-33 0.4180597187700812 1106 34-35 0.5625058313698403 1106 36-37 0.5609186582286299 1106 38-39 0.6568046534840946 1106 40-41 0.456311083009318 1106 42-43 0.5577725592531309 1106 44-45 0.5793457098213395 1106 46-47 0.37697259049837584 1106 48-49 0.39848331300512996 1106 50-51 0.439685166399002 1106 52-53 0.504861409020684 1106 54-55 0.5981415577215685 1106 56-57 0.4522357984189753 1106 58-59 0.48467863736689765 1106 60-61 0.4679970268190843 1106 62-63 0.5322683509331441 1106 64-65 0.6492067577607799 1106 66-67 0.7466522266360087 1106 68-69 0.597571103487752 1106 70-71 0.4804852270695221 1106 72-73 0.7363084761345675 1106 74-75 0.6398354165701505 1106 76-77 0.5448068416079437 1106 78-79 0.6642182532549015 1106 80-81 0.5931337629126041 1106 82-83 0.8155745145647373 1106 84-85 0.6126527397305743 1106 86-87 0.5243844702147307 1106 88-89 0.642572266656579 1106 90-91 0.9109238185788691 1106 92-93 0.8278305325081661 1106 94-95 0.7917743181138874 1106 96-97 1.0740691586103104 1106 98-99 1.0353335542401858 1106 100-101 1.0087826523399173 1107 1 0.1724352671023084 1107 2 0.038659703319680716 1107 3 0.05359965329186167 1107 4 0.10961971528919179 1107 5 0.1372031163721701 1107 6 0.11689116964281965 1107 7 0.13634775267951227 1107 8 0.13713183353532798 1107 9 0.18062129749542066 1107 10-11 0.14909178278490742 1107 12-13 0.1429708311382356 1107 14-15 0.17615006419398327 1107 16-17 0.1828870065624102 1107 18-19 0.13004131796512297 1107 20-21 0.15168479985031524 1107 22-23 0.09507021112181491 1107 24-25 0.17604240616434907 1107 26-27 0.11119615486783374 1107 28-29 0.17465749408756182 1107 30-31 0.19778089363292395 1107 32-33 0.05550769147816936 1107 34-35 0.09610885043698403 1107 36-37 0.10726373330543026 1107 38-39 0.14149625301758562 1107 40-41 0.10976037654702253 1107 42-43 0.01313482326892057 1107 44-45 0.11986358100904226 1107 46-47 -0.02474470878777879 1107 48-49 -0.059560966188421105 1107 50-51 -0.04989575968311044 1107 52-53 0.17048360464967516 1107 54-55 -0.018851955395710718 1107 56-57 0.07235745016252793 1107 58-59 0.16499972358734283 1107 60-61 0.22610305430627875 1107 62-63 0.08942906773344106 1107 64-65 0.06966099655912217 1107 66-67 0.09962259677741514 1107 68-69 0.024561412660993653 1107 70-71 0.09720213628898478 1107 72-73 0.13218924918297148 1107 74-75 0.2521431001971166 1107 76-77 0.27039062916044543 1107 78-79 0.2896601448477618 1107 80-81 0.1384895650688165 1107 82-83 0.16788410191342962 1107 84-85 0.19960473895730857 1107 86-87 0.2810698208307194 1107 88-89 0.17450781193792508 1107 90-91 0.42055368208496446 1107 92-93 0.6694196642197063 1107 94-95 0.6686413024273179 1107 96-97 0.7199617072570241 1107 98-99 0.6111026602711114 1107 100-101 0.6387160760509687 1108 1 0.004760043096077027 1108 2 -0.1329122389402002 1108 3 -0.05560298332851943 1108 4 0.045643048122094854 1108 5 0.013099023673859733 1108 6 -0.00701788397710601 1108 7 0.03967402440059686 1108 8 0.048499831643106006 1108 9 0.04035997079341769 1108 10-11 0.016526052263046154 1108 12-13 -0.027506154892094514 1108 14-15 -0.014633334216540561 1108 16-17 0.04423999179375926 1108 18-19 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-1.1486935733914692 2316 60-61 -0.7388509539045387 2316 62-63 -0.7926576324307248 2316 64-65 -0.8112933511814475 2316 66-67 -0.9342252069412709 2316 68-69 -0.9642721991281462 2316 70-71 -0.7609064138568691 2316 72-73 -0.7162093711668689 2316 74-75 -0.8392938145540043 2316 76-77 -1.3363692218605223 2316 78-79 -1.0107788629800538 2316 80-81 -1.0688325803451484 2316 82-83 -1.0199842309333818 2316 84-85 -0.8776488380093248 2316 86-87 -0.9264813001350376 2316 88-89 -0.9712428904124906 2316 90-91 -1.075767285326389 2316 92-93 -1.4099660146638229 2316 94-95 -1.2833471204362823 2316 96-97 -1.2200338622937785 2316 98-99 -1.1479111300195832 2316 100-101 -1.0579194739561721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 11.0 5 38.0 6 94.0 7 206.0 8 486.0 9 1027.0 10 1822.0 11 2903.0 12 4560.0 13 6253.0 14 8075.0 15 11245.0 16 14892.0 17 20071.0 18 18655.0 19 11722.0 20 9899.0 21 9878.0 22 10491.0 23 11323.0 24 13360.0 25 16014.0 26 19792.0 27 24972.0 28 31226.0 29 39070.0 30 48661.0 31 61764.0 32 79220.0 33 104163.0 34 140984.0 35 199870.0 36 305199.0 37 471901.0 38 378773.0 39 34694.0 40 4.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.29939564314076 19.934737031410464 15.381804930454003 22.384062394994768 2 15.317282303171403 21.021313042408412 40.175510822142094 23.485893832278094 3 19.095876396471255 25.207864767117165 30.474387360270068 25.221871476141512 4 13.025416855547805 17.69370687476873 37.61750457336838 31.66337169631509 5 13.475938106279278 37.565608290351584 34.44906071073676 14.509392892632375 6 30.744617574396223 37.22510579360464 17.67111639402209 14.359160237977054 7 27.498399440123443 33.26718159032008 21.245450884696755 17.98896808485972 8 25.881537387765725 35.18393999976341 19.97388002318621 18.960642589284657 9 25.91641134231602 17.047590881667514 20.9140099132883 36.12198786272817 10-11 24.36375689760702 27.259432831745826 27.621185303622468 20.755624967024687 12-13 25.55346773216638 24.624664980845232 28.229717145409673 21.592150141578717 14-15 22.5141660653236 25.786805153017166 26.92955413862042 24.769474643038812 16-17 21.463026273762907 29.147504524860114 27.416937763950173 21.972531437426802 18-19 21.316716548566834 27.474075213230336 30.239054570404456 20.970153667798375 20-21 22.07373274070036 26.487532893164705 30.522514516526673 20.916219849608265 22-23 21.812428282445907 26.454142169330318 30.378881620195664 21.354547928028108 24-25 21.703794348793895 26.620160056936054 30.49360510455943 21.182440489710626 26-27 21.58622202253316 26.78107146431469 30.32789464556493 21.304811867587222 28-29 21.320684629435092 26.911785582465008 30.461277836139423 21.30625195196048 30-31 21.59241170095457 26.610916284506793 30.423262769819072 21.373409244719568 32-33 21.438701108072696 26.76806846514163 30.356374765829326 21.43685566095635 34-35 21.836905915048284 26.746664294356947 30.191584515189955 21.22484527540481 36-37 21.768715047845827 26.593922335591785 30.150110304242855 21.487252312319534 38-39 21.583177394523336 26.883290891320133 30.24326454359595 21.290267170560583 40-41 21.805475307266164 26.77757256298761 30.175281192372065 21.241670937374156 42-43 21.598185572786242 26.83354437926942 29.892338180765105 21.675931867179234 44-45 21.825499068405644 26.873356401384086 29.699109809836454 21.60203472037382 46-47 21.946111382931708 27.186959677701743 29.20752256400113 21.65940637536542 48-49 22.0030568016202 27.34829557284273 28.95415743947911 21.69449018605796 50-51 21.933208900401052 27.49516696591927 28.81224120998211 21.759382923697565 52-53 22.147313102271493 27.63592890082177 28.505823707596857 21.710934289309876 54-55 21.998831189725827 27.481332159791034 28.649408970027352 21.87042768045579 56-57 21.994068567548418 27.761138588972077 28.499551061305272 21.745241782174237 58-59 22.225008807317657 27.457632010985666 28.62100237093701 21.696356810759667 60-61 22.147604957736775 27.44159261591925 28.8960456687943 21.514756757549673 62-63 21.944211136479243 27.545408302083207 28.89207765014846 21.618302911289085 64-65 21.947783121395926 27.517832718700262 28.72895994715425 21.805424212749557 66-67 22.01122497070965 27.706092392837068 28.38731779887987 21.895364837573414 68-69 21.856742337312056 27.5639336117256 28.607194823323432 21.97212922763891 70-71 22.016895197017966 27.42877625161328 28.604531490106215 21.949797061262537 72-73 22.059555927165942 27.396573503028048 28.561136730303367 21.98273383950264 74-75 21.917343131525588 27.793230067453266 28.218935653227277 22.07049114779387 76-77 22.02423638173378 27.58877599860503 28.408556149226534 21.978431470434657 78-79 21.947835633032383 27.617161622470803 28.343719834194538 22.091282910302272 80-81 21.99157204341273 27.795102699454144 28.205474622670305 22.00785063446282 82-83 22.012995862371813 27.795217689212258 28.263800296031587 21.927986152384342 84-85 21.96886079633172 27.750706553050335 28.118504532704918 22.16192811791303 86-87 21.913258721199156 27.750310003220108 28.22386195516978 22.11256932041095 88-89 22.034004172148812 27.610236977768647 28.0903866205429 22.265372229539636 90-91 21.922438664074225 27.551824829261623 28.328659015833374 22.197077490830782 92-93 22.014802750265165 27.548030389100393 28.17358535027884 22.26358151035561 94-95 21.923589878388007 27.455928930765083 28.187739714700026 22.432741476146887 96-97 21.881599612357434 27.477668674439393 28.209396073510128 22.43133563969305 98-99 21.7267356436604 27.521773180363084 28.020551137694515 22.730940038281993 100-101 21.552417814100057 27.740856909573093 28.03899203308943 22.66773324323742 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 703.0 1 511.5 2 363.5 3 419.0 4 371.0 5 348.5 6 652.0 7 1486.5 8 2561.0 9 3160.5 10 2866.5 11 2423.5 12 2452.0 13 2613.5 14 2782.0 15 3012.5 16 3248.5 17 3457.0 18 3795.0 19 4218.0 20 4559.5 21 5070.5 22 6038.5 23 7282.5 24 8747.5 25 10837.5 26 13742.5 27 17230.5 28 20303.0 29 23629.0 30 27626.5 31 31679.5 32 35927.5 33 40299.5 34 45888.5 35 50773.0 36 56243.0 37 61911.0 38 64194.0 39 65459.5 40 66981.0 41 68892.5 42 70695.0 43 73318.5 44 76142.0 45 78038.5 46 78162.5 47 79285.0 48 83812.0 49 82843.0 50 77300.5 51 73151.5 52 69581.5 53 67046.0 54 63667.5 55 58194.5 56 52522.5 57 47090.5 58 41759.0 59 36815.5 60 31321.5 61 24925.5 62 19291.5 63 14772.0 64 10805.5 65 8002.0 66 6017.5 67 4617.5 68 3304.5 69 2110.5 70 1398.5 71 917.0 72 635.0 73 480.5 74 343.5 75 224.5 76 135.5 77 71.5 78 42.0 79 23.5 80 17.0 81 9.5 82 6.0 83 6.0 84 5.5 85 2.5 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015851797522860894 2 0.0030757219074207705 3 0.002460577525936616 4 1.4195639572711248E-4 5 0.001182969964392604 6 5.678255829084499E-4 7 9.463759715140833E-5 8 0.0 9 0.0 10-11 7.097819786355624E-5 12-13 6.15144381484154E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002082027137330983 22-23 0.0 24-25 0.003643547490329221 26-27 0.0018690925437403144 28-29 0.0030757219074207705 30-31 0.015851797522860894 32-33 7.571007772112666E-4 34-35 0.0025552151230880246 36-37 0.003738185087480629 38-39 0.002342280529497356 40-41 0.004353329468964783 42-43 0.0019400707416038704 44-45 5.91484982196302E-4 46-47 1.4195639572711248E-4 48-49 2.365939928785208E-4 50-51 2.1293459359066874E-4 52-53 0.0011119917665290478 54-55 0.0030757219074207705 56-57 3.5489098931778125E-4 58-59 0.001206629363680456 60-61 0.0010410135686654916 62-63 0.0 64-65 2.1293459359066874E-4 66-67 4.7318798575704164E-5 68-69 0.0 70-71 1.182969964392604E-4 72-73 2.3659399287852082E-5 74-75 4.731879857570416E-4 76-77 6.861225793477104E-4 78-79 2.365939928785208E-4 80-81 0.0056782558290845 82-83 0.001750795547301054 84-85 0.003430612896738552 86-87 0.0019164113423160184 88-89 0.001206629363680456 90-91 1.8927519430281665E-4 92-93 7.097819786355624E-5 94-95 2.602533921663729E-4 96-97 0.0017981143458767584 98-99 0.00283912791454225 100-101 0.0010173541693776396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2113325.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.73912590172148 #Duplication Level Percentage of deduplicated Percentage of total 1 78.76318864283381 47.05244042755288 2 12.674877076896497 15.143721549711264 3 3.7572980718464515 6.733731076929916 4 1.586435722416694 3.7908913342575743 5 0.858204906483972 2.563420547896056 6 0.5193097050683838 1.8613864711839618 7 0.3526520151830126 1.474698619415965 8 0.23710982568903266 1.1331786983497885 9 0.1750090070174316 0.9409396593734644 >10 0.9646655270099471 10.679852309456015 >50 0.0754469406964183 3.1343184575895986 >100 0.03405734261006519 3.541043747926098 >500 0.001269248180626183 0.5034698783700422 >1k 2.3798403386740444E-4 0.27794153206327643 >5k 1.5865602257826962E-4 0.6710878150977081 >10k+ 7.932801128913481E-5 0.4978778748263908 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10506 0.4971312978363479 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8409 0.3979037772230963 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5752 0.27217772940745033 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2318 0.10968497509848225 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.463759715140833E-5 0.0 16-17 0.0 0.0 0.0 1.4195639572711248E-4 0.0 18-19 0.0 0.0 0.0 1.4195639572711248E-4 0.0 20-21 0.0 0.0 0.0 1.6561579501496457E-4 0.0 22-23 0.0 0.0 0.0 2.365939928785208E-4 0.0 24-25 0.0 0.0 0.0 3.548909893177812E-4 0.0 26-27 0.0 0.0 0.0 6.861225793477104E-4 0.0 28-29 0.0 0.0 0.0 0.0010410135686654916 0.0 30-31 0.0 0.0 0.0 0.002318621130209504 0.0 32-33 0.0 0.0 0.0 0.003477931695314256 0.0 34-35 0.0 0.0 0.0 0.004826517454721824 0.0 36-37 0.0 0.0 0.0 0.007334413779234146 0.0 38-39 0.0 0.0 0.0 0.01104893946742692 0.0 40-41 0.0 0.0 0.0 0.01490542155134681 0.0 42-43 0.0 0.0 0.0 0.01921143222173589 0.0 44-45 0.0 0.0 0.0 0.02093856836974909 0.0 46-47 0.0 0.0 0.0 0.02318621130209504 0.0 48-49 0.0 0.0 0.0 0.02709001218459063 0.0 50-51 0.0 0.0 0.0 0.031064791264949786 0.0 52-53 0.0 0.0 0.0 0.04095442016727195 0.0 54-55 0.0 0.0 0.0 0.04814687755077898 0.0 56-57 0.0 0.0 0.0 0.06503968864230537 0.0 58-59 0.0 0.0 0.0 0.09014231128671643 0.0 60-61 4.7318798575704164E-5 0.0 0.0 0.11278435640519087 0.0 62-63 4.7318798575704164E-5 0.0 0.0 0.12352572368187571 0.0 64-65 4.7318798575704164E-5 0.0 0.0 0.14418037926017058 0.0 66-67 4.7318798575704164E-5 0.0 0.0 0.1960181230998545 0.0 68-69 4.7318798575704164E-5 0.0 0.0 0.2713023316337998 0.0 70-71 4.7318798575704164E-5 0.0 0.0 0.33494611571812194 0.0 72-73 4.7318798575704164E-5 0.0 0.0 0.40845586930547834 0.0 74-75 4.7318798575704164E-5 0.0 0.0 0.49675274744774234 0.0 76-77 4.7318798575704164E-5 0.0 0.0 0.5500337146439851 0.0 78-79 4.7318798575704164E-5 0.0 0.0 0.6200418771367395 0.0 80-81 4.7318798575704164E-5 0.0 0.0 0.7209965338980043 0.0 82-83 4.7318798575704164E-5 0.0 0.0 0.8114700767747507 0.0 84-85 4.7318798575704164E-5 0.0 0.0 0.9353033726473685 0.0 86-87 4.7318798575704164E-5 0.0 0.0 1.1293104468077555 0.0 88-89 4.7318798575704164E-5 0.0 0.0 1.3736174038541162 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7145 0.0 38.697906 1 GTATCAA 11045 0.0 33.4212 1 ATCAACG 13510 0.0 27.140362 3 TCAACGC 13535 0.0 27.125322 4 TATCAAC 13790 0.0 26.693878 2 CAACGCA 13780 0.0 26.677519 5 AACGCAG 14005 0.0 26.248928 6 ACGCAGA 15820 0.0 22.967232 7 GAGTACT 9330 0.0 22.72969 12-13 CGCAGAG 15980 0.0 22.618383 8 GTACATG 8130 0.0 22.380768 1 GTGTACG 320 0.0 22.269375 1 GCAGAGT 16690 0.0 21.40007 9 TACATGG 8360 0.0 21.249054 2 GTACTTT 10035 0.0 21.10992 14-15 ACATGGG 8030 0.0 20.70171 3 AGAGTAC 13645 0.0 20.536772 10-11 AGTACTT 9845 0.0 20.213987 12-13 TATACCG 150 7.7287614E-4 18.998255 5 CAGAGTA 16335 0.0 18.841244 10-11 >>END_MODULE