FastQCFastQC Report
Thu 26 May 2016
SRR936818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935928
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84340.435656697976371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61940.3199499154927249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.2175184201065329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62850.032.138491
GTATCAA97000.031.8480931
ATCAACG115900.026.2848283
TCAACGC116250.026.2063674
CAACGCA117300.025.97385
TATCAAC118300.025.9531172
AACGCAG120550.025.3491296
ACGCAGA134650.022.695267
CGCAGAG135050.022.6626458
GTACATG75050.022.417871
GAGTACT68000.021.3495112-13
TACATGG77400.021.000372
GCAGAGT141050.020.9568219
ACATGGG77050.020.3550343
GTACTTT71600.020.3084714-15
AGAGTAC109550.020.17198610-11
AGTACTT73300.019.22234312-13
CATGGGG35950.019.03734
CAGAGTA138000.017.13255310-11
ATTCGCG1701.7511411E-816.76626880-81
Produced by FastQC (version 0.11.5)