##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936818_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1935928 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.694689058684002 33.0 31.0 34.0 30.0 34.0 2 31.81955682236116 33.0 31.0 34.0 30.0 34.0 3 31.826864945390533 33.0 31.0 34.0 30.0 34.0 4 35.42290674033332 37.0 35.0 37.0 33.0 37.0 5 35.4243851010988 37.0 35.0 37.0 33.0 37.0 6 35.49189329355224 37.0 35.0 37.0 33.0 37.0 7 35.42720545392184 37.0 35.0 37.0 33.0 37.0 8 35.37449636556732 37.0 35.0 37.0 33.0 37.0 9 37.00229399027236 39.0 37.0 39.0 33.0 39.0 10-11 36.81033824605048 39.0 37.0 39.0 33.0 39.0 12-13 36.7361593509676 39.0 37.0 39.0 32.0 39.0 14-15 37.87635258129434 40.0 38.0 41.0 33.0 41.0 16-17 37.809880842675966 40.0 38.0 41.0 32.5 41.0 18-19 37.77615903070776 40.0 37.5 41.0 32.0 41.0 20-21 37.703541402366206 40.0 37.5 41.0 32.0 41.0 22-23 37.59192232355748 40.0 37.0 41.0 32.0 41.0 24-25 37.421214528639496 40.0 37.0 41.0 32.0 41.0 26-27 37.52519256914513 40.0 37.0 41.0 32.0 41.0 28-29 37.484045894268796 40.0 37.0 41.0 32.0 41.0 30-31 37.34182262976722 40.0 37.0 41.0 31.5 41.0 32-33 37.1325253315206 40.0 36.0 41.0 31.0 41.0 34-35 36.96777178696729 39.0 36.0 41.0 30.5 41.0 36-37 36.79093514841461 39.0 36.0 41.0 30.0 41.0 38-39 36.62699103479055 39.0 35.5 41.0 30.0 41.0 40-41 36.45973429796976 39.0 35.0 41.0 30.0 41.0 42-43 36.24763885846994 39.0 35.0 41.0 30.0 41.0 44-45 35.994449948551804 39.0 35.0 40.0 29.0 41.0 46-47 35.64940431668947 39.0 35.0 40.0 27.5 41.0 48-49 35.33067422962011 38.5 35.0 40.0 26.5 41.0 50-51 34.68481601588489 38.0 34.0 39.5 25.0 40.5 52-53 34.93043801215748 38.5 34.5 40.0 26.5 40.5 54-55 35.31406488257828 39.0 35.0 40.0 26.5 41.0 56-57 35.20240189717799 39.0 35.0 41.0 26.0 41.0 58-59 34.95835924683149 38.5 34.5 40.0 26.0 41.0 60-61 34.6806802732333 38.0 34.0 40.0 25.5 41.0 62-63 34.35983233880599 38.0 34.0 40.0 24.5 41.0 64-65 34.0447516643181 37.0 34.0 40.0 23.5 41.0 66-67 33.66552991640185 37.0 33.5 39.5 22.5 41.0 68-69 33.28134284952746 36.0 33.0 39.0 22.0 41.0 70-71 32.86445337843143 36.0 32.5 39.0 21.5 40.5 72-73 32.463011279345096 35.0 32.0 38.5 20.0 40.0 74-75 32.02699867970296 35.0 32.0 37.5 19.5 39.5 76-77 31.588595237012946 35.0 31.0 37.0 18.0 39.0 78-79 31.139241748660073 35.0 31.0 36.5 14.5 39.0 80-81 30.697680130666015 35.0 31.0 36.0 10.0 38.5 82-83 30.277356131013136 34.0 30.0 35.5 7.0 37.0 84-85 29.907410554524755 34.0 30.0 35.0 6.0 37.0 86-87 29.57539949832845 34.0 30.0 35.0 2.0 36.0 88-89 29.219189453326777 34.0 29.0 35.0 2.0 36.0 90-91 28.856247494741538 34.0 29.0 35.0 2.0 36.0 92-93 28.472362608526765 34.0 29.0 35.0 2.0 35.0 94-95 28.03975251145704 34.0 29.0 35.0 2.0 35.0 96-97 27.55230928009719 34.0 27.0 35.0 2.0 35.0 98-99 26.93736543921055 34.0 26.5 35.0 2.0 35.0 100-101 24.630644063208962 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2418900694131274 1101 2 0.26378324406895715 1101 3 0.1956123089334021 1101 4 0.1338730354755171 1101 5 0.15440520713285366 1101 6 0.0033277368307196298 1101 7 0.033958177683331314 1101 8 0.032983063332061135 1101 9 -0.015567239385987364 1101 10-11 0.04784588771503451 1101 12-13 0.08289475390201773 1101 14-15 0.44392812993958586 1101 16-17 0.5342776221733345 1101 18-19 0.3677780673532922 1101 20-21 0.32792314319448224 1101 22-23 0.33667929338169245 1101 24-25 0.3204333042030427 1101 26-27 0.364971142170198 1101 28-29 0.5147051349767935 1101 30-31 0.34934309866117985 1101 32-33 0.35899699158260745 1101 34-35 0.2757710585568631 1101 36-37 0.2770719886940256 1101 38-39 0.1488692976494761 1101 40-41 0.258164853161297 1101 42-43 0.2578767618448765 1101 44-45 0.20759563648176282 1101 46-47 0.28323324489226565 1101 48-49 0.31037523963864544 1101 50-51 0.304797805203485 1101 52-53 0.3131378514539591 1101 54-55 0.4005943514188246 1101 56-57 0.3843349350157581 1101 58-59 0.2108693805038513 1101 60-61 0.32667851023177974 1101 62-63 0.32166331587797714 1101 64-65 0.5505850063875002 1101 66-67 0.5528770722150398 1101 68-69 0.3617973371360037 1101 70-71 0.4190140121682546 1101 72-73 0.43661460510179495 1101 74-75 0.4061692903215217 1101 76-77 0.45976405134668497 1101 78-79 0.39466984693433815 1101 80-81 0.19773684733912233 1101 82-83 0.29131803778666665 1101 84-85 0.36113334772228356 1101 86-87 0.3277859794812237 1101 88-89 0.29426238960057205 1101 90-91 0.42788465548867194 1101 92-93 0.4904112416864308 1101 94-95 0.4638887311739097 1101 96-97 0.4138840111160498 1101 98-99 0.4291294552390852 1101 100-101 0.819014853163619 1102 1 0.30880354364146 1102 2 0.1760762743765234 1102 3 0.295513827224287 1102 4 0.24959237217395014 1102 5 0.22407363124244029 1102 6 0.08815389809940655 1102 7 0.2313278797668019 1102 8 0.23938836861287882 1102 9 0.2540078205684466 1102 10-11 0.4813358889299266 1102 12-13 0.48023838974520316 1102 14-15 0.4945581091531679 1102 16-17 0.5254024106061621 1102 18-19 0.3729673918860996 1102 20-21 0.4408033816142449 1102 22-23 0.49868045668907257 1102 24-25 0.6368861985750627 1102 26-27 0.6500805787732702 1102 28-29 0.3486098244868927 1102 30-31 0.4028425486281435 1102 32-33 0.45103869590281676 1102 34-35 0.27896256075449344 1102 36-37 0.248132117040619 1102 38-39 0.46629088673736163 1102 40-41 0.37462467495392815 1102 42-43 0.32178481729086883 1102 44-45 0.41173496792118414 1102 46-47 0.2981046278633741 1102 48-49 0.3211934574650428 1102 50-51 0.29105197205738165 1102 52-53 0.3340662547937683 1102 54-55 0.3612618926463327 1102 56-57 0.43069154864293324 1102 58-59 0.49945656947723194 1102 60-61 0.3108801426898893 1102 62-63 0.38377669091729416 1102 64-65 0.5574300468536251 1102 66-67 0.32090844074050295 1102 68-69 0.33621716045973926 1102 70-71 0.437302922636853 1102 72-73 0.5196006312670605 1102 74-75 0.4834362419069649 1102 76-77 0.3834748438103155 1102 78-79 0.38917534143983445 1102 80-81 0.394009707827113 1102 82-83 0.3136866373077005 1102 84-85 0.31107254036365717 1102 86-87 0.2719131078427921 1102 88-89 0.20760566263829716 1102 90-91 0.24736137935544278 1102 92-93 0.4614167619877243 1102 94-95 0.33338476028488984 1102 96-97 0.24767386770804123 1102 98-99 0.48789769744666955 1102 100-101 0.599373077780136 1103 1 0.30480851555971 1103 2 0.29034497509416823 1103 3 0.3460931400950251 1103 4 0.19672088044689673 1103 5 0.13216913992383894 1103 6 0.2050431389518934 1103 7 0.1682329425938036 1103 8 0.26679557633754314 1103 9 0.2295497190988698 1103 10-11 0.3238706782235923 1103 12-13 0.1521493458685157 1103 14-15 0.4012649958089156 1103 16-17 0.43641076855805494 1103 18-19 0.3023332551771958 1103 20-21 0.5627738237732984 1103 22-23 0.5869629366995923 1103 24-25 0.4439795805897191 1103 26-27 0.5085923053472712 1103 28-29 0.49524996961314827 1103 30-31 0.4974515651871414 1103 32-33 0.4724629890657397 1103 34-35 0.4910771869040147 1103 36-37 0.4660290152863098 1103 38-39 0.45377905200693647 1103 40-41 0.5857825878462108 1103 42-43 0.6371929295965728 1103 44-45 0.7211760408985057 1103 46-47 0.6743350608700922 1103 48-49 0.8063724420154657 1103 50-51 0.6623229821605818 1103 52-53 0.7212202822482894 1103 54-55 0.8105846654330335 1103 56-57 1.0124060861369557 1103 58-59 0.8431330441020535 1103 60-61 1.0155674128830796 1103 62-63 0.9411056409109264 1103 64-65 0.9685885537869012 1103 66-67 0.851287389730409 1103 68-69 0.8531842001978021 1103 70-71 0.6286658534138709 1103 72-73 0.6326490898535937 1103 74-75 0.567634178003722 1103 76-77 0.5333325402668443 1103 78-79 0.7196532494650825 1103 80-81 0.7450736966935558 1103 82-83 0.7628990004068541 1103 84-85 0.7212020249122411 1103 86-87 0.8296108753548133 1103 88-89 0.7908976862245929 1103 90-91 0.9456197945605993 1103 92-93 0.9675827026270518 1103 94-95 0.9625458637918207 1103 96-97 1.0377987760925826 1103 98-99 0.905097806094723 1103 100-101 1.1049196794764704 1104 1 0.35436482207479614 1104 2 0.3299980261715838 1104 3 0.3779965135138674 1104 4 0.41568382593116127 1104 5 0.3553406424140846 1104 6 0.307755863221459 1104 7 0.32210904550709785 1104 8 0.3102476212040983 1104 9 0.2952429006297592 1104 10-11 0.4743603000939274 1104 12-13 0.4323354611698278 1104 14-15 0.41336986421777056 1104 16-17 0.5227468632126673 1104 18-19 0.4429552266426029 1104 20-21 0.48522732655006706 1104 22-23 0.38221693331185236 1104 24-25 0.5085293981402614 1104 26-27 0.3595797656339599 1104 28-29 0.4412948614496983 1104 30-31 0.5750962396012369 1104 32-33 0.4606273705494317 1104 34-35 0.33604929278369156 1104 36-37 0.23539120063473007 1104 38-39 0.31348237149152425 1104 40-41 0.28349649192751514 1104 42-43 0.35333823183906077 1104 44-45 0.4937921891502768 1104 46-47 0.27810118847515497 1104 48-49 0.4191938353731217 1104 50-51 0.44955320556636735 1104 52-53 0.2481353695122266 1104 54-55 0.4536073431000389 1104 56-57 0.4397126180803568 1104 58-59 0.4463851108793975 1104 60-61 0.40920949171135845 1104 62-63 0.5382161555616563 1104 64-65 0.46783621312469137 1104 66-67 0.25237597673730505 1104 68-69 0.3174814116284921 1104 70-71 0.4355980900841061 1104 72-73 0.4891517486436143 1104 74-75 0.3701648741769148 1104 76-77 0.3992816988082559 1104 78-79 0.47001646293515975 1104 80-81 0.5698547994804386 1104 82-83 0.692946277148554 1104 84-85 0.6012402423852556 1104 86-87 0.4723008882484727 1104 88-89 0.4539843265421304 1104 90-91 0.6536844398164909 1104 92-93 0.5899148533395753 1104 94-95 0.5745546618164745 1104 96-97 0.5211120245356504 1104 98-99 0.48619785412991234 1104 100-101 0.6250618046544965 1105 1 0.15589479346152046 1105 2 0.29219018404794994 1105 3 0.28578444877803477 1105 4 0.2373668851484041 1105 5 0.2174287314009149 1105 6 0.2658390004336866 1105 7 0.2129541574435052 1105 8 0.2952297322381199 1105 9 0.42430578181890866 1105 10-11 0.4474601926240993 1105 12-13 0.36154129537606394 1105 14-15 0.4410825705983896 1105 16-17 0.4146961483342224 1105 18-19 0.32873735309424035 1105 20-21 0.4003957639749629 1105 22-23 0.36612128515992737 1105 24-25 0.40011829655178843 1105 26-27 0.3796361532871728 1105 28-29 0.40040655752319054 1105 30-31 0.04724726872692031 1105 32-33 0.1150746717869211 1105 34-35 0.1387726554558384 1105 36-37 0.08567971405938835 1105 38-39 0.109815586925464 1105 40-41 -0.004353408538186443 1105 42-43 0.027024220402815047 1105 44-45 0.03363062163246866 1105 46-47 0.08816730282850216 1105 48-49 0.10330156367849952 1105 50-51 0.056126777056469734 1105 52-53 0.054702479208543764 1105 54-55 0.0933249287017972 1105 56-57 0.31956621877323954 1105 58-59 0.13798387202882623 1105 60-61 0.22004784710548364 1105 62-63 0.2930644281087851 1105 64-65 0.2091285492105257 1105 66-67 0.2227758597448357 1105 68-69 0.31936054660969404 1105 70-71 0.20999986401654525 1105 72-73 0.2031977611500153 1105 74-75 0.17141914236176348 1105 76-77 0.38406530807151995 1105 78-79 0.4372102759376517 1105 80-81 0.48039296487515415 1105 82-83 0.47498974574877195 1105 84-85 0.2967331978963692 1105 86-87 0.22218536951380585 1105 88-89 0.3639164436978568 1105 90-91 0.46904979190489726 1105 92-93 0.35404033457316686 1105 94-95 0.3004308583819757 1105 96-97 0.20283308363498875 1105 98-99 0.38977349339403844 1105 100-101 0.469258320273898 1106 1 0.24243529825654164 1106 2 0.2808977708696041 1106 3 0.1544136228720383 1106 4 0.10068930323587466 1106 5 0.18108122442927055 1106 6 0.16211822703366607 1106 7 0.23882926294921702 1106 8 0.2885806413303271 1106 9 0.247377903586127 1106 10-11 0.36502367273857317 1106 12-13 0.3630281912510043 1106 14-15 0.5872277659746317 1106 16-17 0.5441867297406091 1106 18-19 0.4863296359416509 1106 20-21 0.47967966483207647 1106 22-23 0.33601976409293144 1106 24-25 0.4887724850483508 1106 26-27 0.30634231187020333 1106 28-29 0.2682027510602367 1106 30-31 0.49104803015277554 1106 32-33 0.43453431595514047 1106 34-35 0.36240372252522945 1106 36-37 0.372433479010148 1106 38-39 0.4078915101108791 1106 40-41 0.4673628502761673 1106 42-43 0.3806608152934743 1106 44-45 0.30442663979265916 1106 46-47 0.0923199506501291 1106 48-49 0.4253370107431351 1106 50-51 0.48497303295105354 1106 52-53 0.5489456210098922 1106 54-55 0.5355583636204528 1106 56-57 0.35283213791094425 1106 58-59 0.4532846814037086 1106 60-61 0.611520272605425 1106 62-63 0.4984751014440505 1106 64-65 0.46621376213396815 1106 66-67 0.5144392247102445 1106 68-69 0.4340728321984031 1106 70-71 0.33574613773436823 1106 72-73 0.4354584639566852 1106 74-75 0.5582807271926384 1106 76-77 0.6836575152475071 1106 78-79 0.5029566067659736 1106 80-81 0.6783501219997241 1106 82-83 0.6679926852742142 1106 84-85 0.6297383837684656 1106 86-87 0.5790062321727873 1106 88-89 0.5623886662175259 1106 90-91 0.6210906954026534 1106 92-93 0.6732991401211663 1106 94-95 0.7033570342456947 1106 96-97 0.5593023597283668 1106 98-99 0.5537986800799608 1106 100-101 0.5890599849976184 1107 1 0.16376753140658806 1107 2 0.25946031550726545 1107 3 0.25157646319383176 1107 4 0.18216860570196758 1107 5 0.19903821630186513 1107 6 0.25037024643968664 1107 7 0.27385150088985455 1107 8 0.3526237295773669 1107 9 0.36516538790294817 1107 10-11 0.5419657621212721 1107 12-13 0.37794679989050195 1107 14-15 0.22582881544708755 1107 16-17 0.32569288887859926 1107 18-19 0.283708017055595 1107 20-21 0.3744877937042759 1107 22-23 0.23634879980566836 1107 24-25 0.526131585300071 1107 26-27 0.3883704812715649 1107 28-29 0.5009933251980456 1107 30-31 0.38490977541925275 1107 32-33 0.4257204445433729 1107 34-35 0.551754992860026 1107 36-37 0.38205636914360497 1107 38-39 0.09930953527555886 1107 40-41 0.4608991154351898 1107 42-43 0.37364489495229947 1107 44-45 0.3954605647708078 1107 46-47 0.11034011377655872 1107 48-49 0.19337489263588026 1107 50-51 -0.14393342168144585 1107 52-53 0.08440294651678215 1107 54-55 0.26926166163398335 1107 56-57 0.3585039209355827 1107 58-59 0.44899958443879484 1107 60-61 0.32019068719611 1107 62-63 0.23393048322005683 1107 64-65 0.3487090669571913 1107 66-67 0.3850278741278075 1107 68-69 0.3237063701844747 1107 70-71 0.25720815047717593 1107 72-73 0.20193152396510783 1107 74-75 0.3478880959238424 1107 76-77 0.2566662992518296 1107 78-79 0.20212845888787712 1107 80-81 0.16051781335232462 1107 82-83 0.22567598087467644 1107 84-85 0.1759242741311411 1107 86-87 0.22982054874633917 1107 88-89 0.15901994354976168 1107 90-91 0.15591891581510353 1107 92-93 0.3809362958170901 1107 94-95 0.41542862232835276 1107 96-97 0.22155695481485083 1107 98-99 0.27385058717626265 1107 100-101 0.2312380919032897 1108 1 0.016933171407274017 1108 2 0.17528299815232629 1108 3 0.10100787071529638 1108 4 0.11846174213160054 1108 5 0.1208400285725233 1108 6 -0.10716207227761032 1108 7 0.08728724076760841 1108 8 0.1443267003058537 1108 9 -0.06519512402356753 1108 10-11 -0.030480667374739312 1108 12-13 0.06215241859957388 1108 14-15 0.21636613586644415 1108 16-17 0.13020175378578358 1108 18-19 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-2.1938268248638586 2316 74-75 -2.321866946797762 2316 76-77 -2.6161593044209646 2316 78-79 -2.681017441555717 2316 80-81 -2.6727073710722244 2316 82-83 -2.576570633518955 2316 84-85 -2.864954938270845 2316 86-87 -3.2323557242590937 2316 88-89 -3.222318564047857 2316 90-91 -2.7248469122515466 2316 92-93 -2.7968873881727525 2316 94-95 -3.1727744182487534 2316 96-97 -3.3812690048414673 2316 98-99 -3.292561483579881 2316 100-101 -3.246657003814466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4354.0 3 1546.0 4 1676.0 5 1785.0 6 1904.0 7 2225.0 8 2707.0 9 3509.0 10 4261.0 11 5388.0 12 6576.0 13 8269.0 14 9313.0 15 11157.0 16 14171.0 17 17788.0 18 16084.0 19 11363.0 20 10900.0 21 11398.0 22 12496.0 23 13843.0 24 15711.0 25 18121.0 26 21014.0 27 24963.0 28 29620.0 29 35808.0 30 43843.0 31 54502.0 32 69341.0 33 90194.0 34 121934.0 35 174106.0 36 260447.0 37 387312.0 38 353296.0 39 62964.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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18-19 21.476628689304246 27.72070966529946 29.787590857422224 21.015070787974068 20-21 22.29222215962742 26.804371207379617 29.859037658352644 21.04436897464032 22-23 21.975877343123287 26.782091310598904 29.73047058170042 21.51156076457739 24-25 21.80843094383917 26.97315125564171 29.82727693553987 21.391140864979253 26-27 21.663077008120773 27.103101640634968 29.745000782681608 21.48882056856265 28-29 21.441273278945403 27.2410222974673 29.815468167251414 21.502236256335888 30-31 21.64906858048706 26.95745074907997 29.81394857451498 21.579532095917987 32-33 21.479903780114885 27.139705580376226 29.723245140381792 21.657145499127093 34-35 21.89296580554355 27.13239105991424 29.58715116454776 21.38749196999445 36-37 21.870619549256702 26.917518703483438 29.562726569471838 21.649135177788022 38-39 21.61046237710101 27.278952295147686 29.62581157761182 21.48477375013948 40-41 21.870972207944234 27.084016739600646 29.578684233501 21.46632681895412 42-43 21.691480530402472 27.17323419152573 29.30582654419616 21.829458733875637 44-45 21.778147287794752 27.297810782288522 29.170276104565477 21.753765825351245 46-47 21.96903913138481 27.422782500621167 28.765077900657225 21.84310046733679 48-49 21.952540506280403 27.578957318895288 28.63546659221392 21.833035582610393 50-51 21.873529355548428 27.729709820379266 28.511944151284148 21.884816672788162 52-53 22.105584795268772 27.847290435544448 28.23771960026211 21.80940516892467 54-55 21.960760712566202 27.683152491729363 28.315977567513396 22.040109228191042 56-57 22.014257947116015 28.00669290646981 28.185304745794586 21.793744400619588 58-59 22.173110854705687 27.66985589205969 28.241989547647627 21.915043705586996 60-61 22.07802876440765 27.691696843789025 28.578772493344157 21.651501898459173 62-63 21.934364293745965 27.740691249941555 28.60423059367749 21.72071386263499 64-65 21.85069761494858 27.78071527003762 28.425416612315168 21.943170502698635 66-67 21.899626207334812 27.927581868859995 28.16609103801732 22.006700885787872 68-69 21.76935809429181 27.845537749456117 28.3652533306672 22.01985082558488 70-71 21.938657286316886 27.73958421325778 28.273028316969643 22.04873018345569 72-73 21.919031889663195 27.6808651946241 28.315460425111123 22.08464249060158 74-75 21.77729763465793 28.058871608558672 28.057424979359702 22.1064057774237 76-77 21.946326115312488 27.828049914992192 28.13886253071377 22.08676143898155 78-79 21.869458688737303 27.868046417375535 28.089136904800355 22.173357989086806 80-81 21.878161651256498 28.063501200329462 27.978010667069015 22.08032648134503 82-83 21.92930535902654 27.986044710354623 28.086208843121057 21.998441087497774 84-85 21.882302733518262 28.019014296630672 27.912736354754426 22.185946615096636 86-87 21.867662342661383 27.974659966710334 28.035292315347114 22.12238537528117 88-89 21.965437079100713 27.843541261789184 27.92876412383587 22.26225753527423 90-91 21.90841309659705 27.819432141876444 28.08142194695468 22.19073281457182 92-93 21.97492793110736 27.769452917143123 28.072635729532514 22.18298342221701 94-95 21.842736082267024 27.69521458407552 28.068098768583177 22.393950565074284 96-97 21.845965353237 27.71836638094077 28.079649109027134 22.3560191567951 98-99 21.69365260789445 27.860541210745655 27.863280419233277 22.582525762126618 100-101 21.678089077749068 27.758940003008302 27.975878803730087 22.587092115512547 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 376.0 1 285.5 2 258.5 3 352.5 4 341.0 5 357.5 6 586.5 7 1196.5 8 2113.5 9 2578.5 10 2321.0 11 2040.5 12 2119.0 13 2267.5 14 2401.0 15 2572.0 16 2673.0 17 2843.0 18 3097.0 19 3323.0 20 3750.5 21 4390.0 22 5300.5 23 6503.0 24 7799.0 25 9658.0 26 12519.0 27 15929.5 28 18549.0 29 21318.0 30 25341.5 31 29181.5 32 33029.5 33 37317.0 34 42685.5 35 47263.5 36 52059.5 37 56913.5 38 58854.5 39 60386.5 40 61565.0 41 63295.0 42 65376.5 43 67491.5 44 70016.5 45 71890.5 46 72021.0 47 72604.0 48 76398.5 49 75920.0 50 71125.5 51 67342.0 52 63686.5 53 61247.5 54 58303.5 55 53230.5 56 48149.0 57 43021.0 58 38075.0 59 33526.0 60 28253.0 61 22723.5 62 17751.5 63 13497.5 64 10027.5 65 7513.0 66 5650.5 67 4242.5 68 3016.0 69 2004.0 70 1388.5 71 944.0 72 635.5 73 442.5 74 295.0 75 202.0 76 136.0 77 66.0 78 43.5 79 32.0 80 26.5 81 19.5 82 9.0 83 10.0 84 10.0 85 7.0 86 5.0 87 5.0 88 3.0 89 2.0 90 2.5 91 1.5 92 1.5 93 2.5 94 1.5 95 2.5 96 2.0 97 0.5 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0409106123781463 2 0.041892053836712934 3 0.02427776239612217 4 0.044164865635498846 5 0.06554995846952986 6 0.06369038517961412 7 0.06539499402870354 8 0.06730622213222806 9 0.06704794806418421 10-11 0.0735564545788893 12-13 0.07275580496795335 14-15 0.06784859767512015 16-17 0.05583885351108099 18-19 0.049562793657615366 20-21 0.056045472765516066 22-23 0.03153526370815443 24-25 0.017769255881417076 26-27 0.014205073742411907 28-29 0.016891124050067978 30-31 0.013094495249823342 32-33 0.007929013888946283 34-35 0.011338231587125141 36-37 0.006482679107900707 38-39 0.00991772421288395 40-41 0.00821311536379452 42-43 0.005940303565008616 44-45 0.0016012992218718879 46-47 0.003357562884570087 48-49 0.001265542933414879 50-51 0.006560161328313862 52-53 0.005656202090160377 54-55 0.008471389431838373 56-57 0.023038046869511677 58-59 0.009891896806079565 60-61 0.021230128393204706 62-63 0.01495406853973908 64-65 0.011648160468777764 66-67 0.01875069733998372 68-69 0.028022736382758033 70-71 0.02027451434144245 72-73 0.018776524746788103 74-75 0.020351996561855604 76-77 0.02673136604253877 78-79 0.038611973172556005 80-81 0.03233591331909038 82-83 0.031225334826501812 84-85 0.02257315354703274 86-87 0.025439995702319505 88-89 0.021410920240835404 90-91 0.018414941051526707 92-93 0.03238756813269915 94-95 0.02451020905736164 96-97 0.03822456207049023 98-99 0.05488323945931873 100-101 0.0661698162328351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1935928.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.947319780229535 #Duplication Level Percentage of deduplicated Percentage of total 1 80.76487477332938 50.83932399366958 2 11.614402634159031 14.621910333374977 3 3.4153731369276588 6.4496575505697304 4 1.3984513047481995 3.5211504590825657 5 0.7557258852822013 2.378545948352789 6 0.44203783696414173 1.6695058247005679 7 0.28430338003294153 1.2527295044273588 8 0.2152926265389369 1.0841675047257595 9 0.16026688574379447 0.9079533816386527 >10 0.8538525870943553 9.91772222217664 >50 0.06730733838856191 2.906834057316351 >100 0.026961336947138623 2.9779547238799937 >500 5.751369218101371E-4 0.21851294239811866 >1k 4.108120870072387E-4 0.49748544211301654 >5k 1.643248348028955E-4 0.7565461115738856 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8434 0.435656697976371 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6194 0.3199499154927249 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4211 0.2175184201065329 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.1654813608770576E-5 0.0 14-15 0.0 0.0 0.0 5.1654813608770576E-5 0.0 16-17 0.0 0.0 0.0 5.1654813608770576E-5 0.0 18-19 0.0 2.5827406804385288E-5 0.0 5.1654813608770576E-5 0.0 20-21 0.0 5.1654813608770576E-5 0.0 5.1654813608770576E-5 0.0 22-23 0.0 5.1654813608770576E-5 0.0 1.2913703402192645E-4 0.0 24-25 0.0 5.1654813608770576E-5 0.0 3.6158369526139404E-4 0.0 26-27 0.0 5.1654813608770576E-5 0.0 6.198577633052468E-4 0.0 28-29 0.0 5.1654813608770576E-5 0.0 0.0013946799674368054 0.0 30-31 0.0 5.1654813608770576E-5 0.0 0.002918496968895537 0.0 32-33 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.004623105817984967 0.0 34-35 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.006095268005834928 0.0 36-37 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.008393907211425218 0.0 38-39 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.012474637486518093 0.0 40-41 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.015858027777892567 0.0 42-43 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.019654656578137204 0.0 44-45 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.021436747647639788 0.0 46-47 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.023632077226012536 0.0 48-49 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.02784194453512734 0.0 50-51 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.03236174072589476 0.0 52-53 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.043338388617758516 0.0 54-55 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.050389270675355695 0.0 56-57 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.07012140947390605 0.0 58-59 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.09775673475459831 0.0 60-61 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.12100140087854508 0.0 62-63 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.13347603836506317 0.0 64-65 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.15646243042096608 0.0 66-67 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.21041588323532695 0.0 68-69 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.2928569657549248 0.0 70-71 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.3614803856341765 0.0 72-73 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.4449545644259497 0.0 74-75 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.5403093503477402 0.0 76-77 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.5984984978780203 0.0 78-79 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.6764197842068507 0.0 80-81 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.783835969106289 0.0 82-83 5.1654813608770576E-5 5.1654813608770576E-5 0.0 0.8810245009111909 0.0 84-85 5.1654813608770576E-5 1.0330962721754115E-4 0.0 1.0136998896653182 0.0 86-87 5.1654813608770576E-5 1.0330962721754115E-4 0.0 1.2199317329983346 0.0 88-89 5.1654813608770576E-5 1.2913703402192645E-4 0.0 1.4861089875243294 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6285 0.0 32.13849 1 GTATCAA 9700 0.0 31.848093 1 ATCAACG 11590 0.0 26.284828 3 TCAACGC 11625 0.0 26.206367 4 CAACGCA 11730 0.0 25.9738 5 TATCAAC 11830 0.0 25.953117 2 AACGCAG 12055 0.0 25.349129 6 ACGCAGA 13465 0.0 22.69526 7 CGCAGAG 13505 0.0 22.662645 8 GTACATG 7505 0.0 22.41787 1 GAGTACT 6800 0.0 21.34951 12-13 TACATGG 7740 0.0 21.00037 2 GCAGAGT 14105 0.0 20.956821 9 ACATGGG 7705 0.0 20.355034 3 GTACTTT 7160 0.0 20.30847 14-15 AGAGTAC 10955 0.0 20.171986 10-11 AGTACTT 7330 0.0 19.222343 12-13 CATGGGG 3595 0.0 19.0373 4 CAGAGTA 13800 0.0 17.132553 10-11 ATTCGCG 170 1.7511411E-8 16.766268 80-81 >>END_MODULE