FastQCFastQC Report
Thu 26 May 2016
SRR936818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935928
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99480.5138620857800497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79380.4100359104264208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.2750102276530945No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21180.10940489522337608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66400.039.5716481
GTATCAA105350.034.0066531
ATCAACG126750.027.9543653
TCAACGC127000.027.8986174
TATCAAC129550.027.4608762
CAACGCA129200.027.38685
AACGCAG132200.026.835096
ACGCAGA147850.023.8982187
CGCAGAG148800.023.5860618
GAGTACT86050.022.68410712-13
GCAGAGT157200.021.66119
GTACATG77600.021.1244051
GTACTTT93000.020.78542914-15
AGAGTAC130550.020.42616310-11
TACATGG79400.020.0398432
AGTACTT92900.019.73341812-13
ACATGGG76950.019.4428843
TAGACCG1759.9493336E-518.9979865
CAGAGTA155200.018.94143510-11
GTATAGC8600.018.7848421
Produced by FastQC (version 0.11.5)