##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936818_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1935928 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.248620816476645 33.0 31.0 34.0 31.0 34.0 2 32.32780764573889 33.0 31.0 34.0 31.0 34.0 3 32.462109128025425 34.0 31.0 34.0 31.0 34.0 4 36.12204792740226 37.0 35.0 37.0 35.0 37.0 5 36.04054851213475 37.0 35.0 37.0 35.0 37.0 6 36.074870553037094 37.0 35.0 37.0 35.0 37.0 7 36.03088389650855 37.0 35.0 37.0 35.0 37.0 8 36.01567981867094 37.0 35.0 37.0 35.0 37.0 9 37.72314724514548 39.0 37.0 39.0 35.0 39.0 10-11 37.62763620341252 39.0 37.0 39.0 35.0 39.0 12-13 37.55532488811568 39.0 37.0 39.0 35.0 39.0 14-15 38.800835826539 40.0 38.0 41.0 35.0 41.0 16-17 38.722560446462886 40.0 38.0 41.0 35.0 41.0 18-19 38.76368852560633 40.0 38.0 41.0 34.5 41.0 20-21 38.860409323073995 40.0 38.0 41.0 35.0 41.0 22-23 38.80178033480584 40.0 38.0 41.0 35.0 41.0 24-25 38.71868685199036 40.0 38.0 41.0 34.5 41.0 26-27 38.55831441045328 40.0 38.0 41.0 34.0 41.0 28-29 38.416248176585086 40.0 38.0 41.0 34.0 41.0 30-31 38.358151232897086 40.0 38.0 41.0 34.0 41.0 32-33 38.26759053022633 40.0 38.0 41.0 34.0 41.0 34-35 38.15648025133166 40.0 38.0 41.0 34.0 41.0 36-37 37.99240028554781 40.0 37.0 41.0 33.0 41.0 38-39 37.857818834171525 40.0 37.0 41.0 33.0 41.0 40-41 37.64398830948258 40.0 37.0 41.0 33.0 41.0 42-43 37.429068126500574 40.0 37.0 41.0 33.0 41.0 44-45 37.18977152042845 40.0 37.0 41.0 32.0 41.0 46-47 36.84117952733779 40.0 36.0 41.0 31.0 41.0 48-49 36.47807356471935 39.5 36.0 41.0 30.0 41.0 50-51 36.243852044084285 39.0 36.0 41.0 30.0 41.0 52-53 36.03123437441889 39.0 35.0 40.5 29.5 41.0 54-55 35.768296134980226 39.0 35.0 40.0 28.5 41.0 56-57 35.532322741341616 38.5 35.0 40.0 28.0 41.0 58-59 35.199359428656436 38.0 34.5 40.0 27.5 41.0 60-61 34.878266133864486 38.0 34.0 40.0 27.0 41.0 62-63 34.55582154914852 37.5 34.0 40.0 26.0 41.0 64-65 34.16060540474646 37.0 34.0 39.5 26.0 41.0 66-67 33.7354392828659 36.5 33.0 39.0 25.5 40.5 68-69 33.30368200676885 36.0 33.0 39.0 24.5 40.0 70-71 32.908316838229524 35.0 32.0 38.5 24.0 40.0 72-73 32.52190758127368 35.0 32.0 38.0 22.5 40.0 74-75 32.04621814447645 35.0 31.5 37.0 21.5 39.0 76-77 30.591340690356255 33.5 29.5 35.5 20.0 38.5 78-79 31.288132099954133 34.0 31.0 36.0 20.5 38.5 80-81 30.98225373051064 34.0 31.0 36.0 18.0 37.5 82-83 30.776933594637818 34.0 31.0 35.5 18.5 37.0 84-85 30.441269510023098 34.0 31.0 35.0 18.0 37.0 86-87 30.077723964940844 34.0 30.0 35.0 13.5 36.0 88-89 29.70749480352575 34.0 30.0 35.0 7.5 36.0 90-91 29.26234007669707 34.0 29.5 35.0 4.5 35.5 92-93 28.843637779917437 34.0 29.0 35.0 2.0 35.0 94-95 28.365945685996586 34.0 29.0 35.0 2.0 35.0 96-97 27.751640298606148 33.0 28.0 35.0 2.0 35.0 98-99 26.98722963870557 33.0 26.5 35.0 2.0 35.0 100-101 23.72440013264956 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2263553997025909 1101 2 0.21856785088226616 1101 3 0.23423403502389561 1101 4 0.15277395020219586 1101 5 0.21195782689176923 1101 6 0.22347939000404438 1101 7 0.17135465295987728 1101 8 0.11315874159274131 1101 9 0.26689175353564565 1101 10-11 0.20863624027612104 1101 12-13 0.18861031421526064 1101 14-15 0.21821328008906704 1101 16-17 0.2221583663764406 1101 18-19 0.23443919461413287 1101 20-21 0.1876983416854756 1101 22-23 0.24308428113491232 1101 24-25 0.33219618045389154 1101 26-27 0.22490890561751797 1101 28-29 0.21213009703083685 1101 30-31 0.14447686287003592 1101 32-33 0.28003127146867257 1101 34-35 0.26980589221016515 1101 36-37 0.3511407076121671 1101 38-39 0.2223660805131331 1101 40-41 0.2933589977283617 1101 42-43 0.22617477708696043 1101 44-45 0.2960544019865097 1101 46-47 0.30233094390867876 1101 48-49 0.3704248512136914 1101 50-51 0.2553017777252009 1101 52-53 0.2647500489340473 1101 54-55 0.2902659852033409 1101 56-57 0.24377810066064143 1101 58-59 0.2285089990879996 1101 60-61 0.13904468611799103 1101 62-63 0.055275820945247744 1101 64-65 0.20610112521663382 1101 66-67 0.049737231155077666 1101 68-69 0.11024319265508353 1101 70-71 0.24463737765383797 1101 72-73 0.14736696235213742 1101 74-75 0.09358906084503715 1101 76-77 -0.028715862390757962 1101 78-79 0.1945636783117095 1101 80-81 0.6774459751772994 1101 82-83 0.4570738822043481 1101 84-85 0.3884180557051131 1101 86-87 0.3502945323028719 1101 88-89 0.1616469329884005 1101 90-91 0.2897062181503607 1101 92-93 0.3000647295397876 1101 94-95 0.3659515216679061 1101 96-97 0.48899470327435424 1101 98-99 0.7575903163837872 1101 100-101 1.0148116984917728 1102 1 0.25686247024827935 1102 2 0.27815074111031635 1102 3 0.21900099076994906 1102 4 0.1720908441215414 1102 5 0.2046278669834365 1102 6 0.22669283943511687 1102 7 0.1598455653490234 1102 8 0.15854998899443729 1102 9 0.22205012310791972 1102 10-11 0.222462061206663 1102 12-13 0.2524286044363251 1102 14-15 0.17688611944113575 1102 16-17 0.29654721393657013 1102 18-19 0.32113752770614923 1102 20-21 0.12875972237657152 1102 22-23 0.1914539865468683 1102 24-25 0.1591972688702441 1102 26-27 0.24873205543453736 1102 28-29 0.25995831946720216 1102 30-31 0.29542702205508675 1102 32-33 0.17655329026465694 1102 34-35 0.13352293391448455 1102 36-37 0.13576314446654436 1102 38-39 0.23841013225118957 1102 40-41 0.4850928374915 1102 42-43 0.2983737900231418 1102 44-45 0.17697172977457853 1102 46-47 0.39508130463381974 1102 48-49 0.4025692269802832 1102 50-51 0.3027024971783945 1102 52-53 0.1398117300214068 1102 54-55 0.2075002634950991 1102 56-57 0.2940369531968585 1102 58-59 0.28730719770901203 1102 60-61 0.35325405315562364 1102 62-63 0.30867837023603073 1102 64-65 0.26015185640275007 1102 66-67 0.15956482783523995 1102 68-69 0.25228974408940985 1102 70-71 0.21812730397770963 1102 72-73 0.2850544395727894 1102 74-75 0.3500888744495718 1102 76-77 0.3933048890825148 1102 78-79 0.41266061809765375 1102 80-81 0.34813928308096465 1102 82-83 0.3986096767898921 1102 84-85 0.3559031773531167 1102 86-87 0.23137818059460713 1102 88-89 0.2011067065179155 1102 90-91 0.18074798155128136 1102 92-93 0.4152324729730914 1102 94-95 0.49122237757256215 1102 96-97 0.4894027553918576 1102 98-99 0.638542593320846 1102 100-101 1.1792016777386323 1103 1 0.20574770111924323 1103 2 0.17135589541963725 1103 3 0.19408260995362525 1103 4 0.11626942677251861 1103 5 0.1834337134938977 1103 6 0.18702564987582093 1103 7 0.2048699804337275 1103 8 0.17709574387509974 1103 9 0.21306269260559674 1103 10-11 0.20090912744306166 1103 12-13 0.29227492808050926 1103 14-15 0.3654530631494026 1103 16-17 0.30464696284159487 1103 18-19 0.3427106383792591 1103 20-21 0.297505464229296 1103 22-23 0.30833380468019556 1103 24-25 0.24764198352367828 1103 26-27 0.30690597752667514 1103 28-29 0.23807231306548715 1103 30-31 0.25244918060339927 1103 32-33 0.18219084168504907 1103 34-35 0.25541563864047134 1103 36-37 0.23635620221674714 1103 38-39 0.05966360790309011 1103 40-41 0.20995609540074867 1103 42-43 0.10503864315776212 1103 44-45 0.22450258815561597 1103 46-47 0.19808707010889748 1103 48-49 0.2516239234069033 1103 50-51 0.2294487421223934 1103 52-53 0.20569753322013185 1103 54-55 0.19385719036127824 1103 56-57 -0.010171642863049613 1103 58-59 0.2149065059994868 1103 60-61 0.10588401600962527 1103 62-63 0.008151394424970704 1103 64-65 0.11928025897736916 1103 66-67 0.01748343520651474 1103 68-69 0.03643296696252918 1103 70-71 0.16429592394674586 1103 72-73 0.22135265880177712 1103 74-75 0.15743585051464137 1103 76-77 0.25610620613284496 1103 78-79 0.4116794262547785 1103 80-81 0.5603999613774526 1103 82-83 0.4650272490959466 1103 84-85 0.5574304567396062 1103 86-87 0.5236625304003546 1103 88-89 0.2721978451060423 1103 90-91 0.2842354528171036 1103 92-93 0.40216951159272085 1103 94-95 0.526881326759117 1103 96-97 0.6791643360161252 1103 98-99 0.841427973316577 1103 100-101 0.8273205752477146 1104 1 0.16482635242588373 1104 2 0.07974857047420159 1104 3 0.05098029499445289 1104 4 0.11567320048462193 1104 5 0.02209698679728689 1104 6 0.09813020647728621 1104 7 0.008094237363017953 1104 8 0.055410804171906136 1104 9 0.1763376814395201 1104 10-11 0.06023195764286271 1104 12-13 0.06171590799842619 1104 14-15 0.08288583494853441 1104 16-17 0.20571764199178943 1104 18-19 0.11278682050848232 1104 20-21 0.14651779326194259 1104 22-23 0.15974410106576187 1104 24-25 0.14901604283449643 1104 26-27 0.22622476265767233 1104 28-29 0.16529457274204162 1104 30-31 0.2448859553351994 1104 32-33 0.24846781934447648 1104 34-35 0.2770540179162353 1104 36-37 0.2221007055581481 1104 38-39 0.2903905404768494 1104 40-41 0.3055560296683808 1104 42-43 0.138048300759543 1104 44-45 0.16809789587578905 1104 46-47 0.17256689954182747 1104 48-49 0.30901776141875104 1104 50-51 0.13841130838145688 1104 52-53 0.11209822256726909 1104 54-55 0.057057176877712834 1104 56-57 -0.07863169359495004 1104 58-59 -0.09226485322790978 1104 60-61 0.021414341384847546 1104 62-63 -0.011423367716552946 1104 64-65 -0.0016913159870242112 1104 66-67 -0.07220185001297352 1104 68-69 0.07166825513154862 1104 70-71 0.15364896575422193 1104 72-73 0.19832336576181575 1104 74-75 0.09183073350260074 1104 76-77 0.19831333893750624 1104 78-79 0.2996246998446921 1104 80-81 0.335606428431209 1104 82-83 0.3347513838304472 1104 84-85 0.1093600809695694 1104 86-87 0.21858301951659342 1104 88-89 0.274416523211265 1104 90-91 0.17780274000856267 1104 92-93 0.23119983275432787 1104 94-95 0.2352002635802002 1104 96-97 0.2574659127502379 1104 98-99 0.37947205613935964 1104 100-101 0.4921156250947618 1105 1 0.23015236692750562 1105 2 0.19580737506502288 1105 3 0.16844459641786358 1105 4 0.13551997661634374 1105 5 0.10177113100638024 1105 6 0.12093194957450493 1105 7 0.13695600939092856 1105 8 0.1418582686547154 1105 9 0.059130264660623766 1105 10-11 0.16147273541611185 1105 12-13 0.18846535364453842 1105 14-15 0.30225908629643783 1105 16-17 0.26660361060334736 1105 18-19 0.22821055547063906 1105 20-21 0.3265812322059247 1105 22-23 0.15968696520484826 1105 24-25 0.2677010564609503 1105 26-27 0.34501123778316867 1105 28-29 0.2325742029007074 1105 30-31 0.25304266630483596 1105 32-33 0.25696621183156054 1105 34-35 0.3061226807103665 1105 36-37 0.3206346719205371 1105 38-39 0.4980034404845526 1105 40-41 0.5500801695696182 1105 42-43 0.5595670943766535 1105 44-45 0.5677648300834051 1105 46-47 0.7290325477391022 1105 48-49 0.748875579282327 1105 50-51 0.665783126798253 1105 52-53 0.6407147865254217 1105 54-55 0.509436704101141 1105 56-57 0.4611846327505731 1105 58-59 0.5936936688578243 1105 60-61 0.6606674713870717 1105 62-63 0.7258840821648604 1105 64-65 0.637465794026717 1105 66-67 0.5974765793856989 1105 68-69 0.5998036988640578 1105 70-71 0.6281287346713995 1105 72-73 0.6842945847055262 1105 74-75 0.5173867878632912 1105 76-77 0.6015400413367615 1105 78-79 0.6824519726956382 1105 80-81 0.8864078042264651 1105 82-83 0.7355824612041957 1105 84-85 0.5737799705975952 1105 86-87 0.5749967466339072 1105 88-89 0.5249437829118904 1105 90-91 0.5493745883536647 1105 92-93 0.7323061598707383 1105 94-95 0.7653235591403806 1105 96-97 0.8628146909043863 1105 98-99 0.9280802764479823 1105 100-101 1.2095587272999317 1106 1 0.12188726280434281 1106 2 0.015798860840106954 1106 3 0.04655697038508322 1106 4 -0.016308071258443135 1106 5 -0.013518327655006601 1106 6 0.06017683919535699 1106 7 0.048949430260393 1106 8 0.10622369119275987 1106 9 0.1435031877120636 1106 10-11 0.11006019028393865 1106 12-13 0.07528232089094189 1106 14-15 0.15551982265553477 1106 16-17 0.09268807371340415 1106 18-19 0.08914480247691614 1106 20-21 -0.013540279918522913 1106 22-23 0.04197792468279715 1106 24-25 -0.027258060743854173 1106 26-27 0.08510452779817967 1106 28-29 0.03484774843289529 1106 30-31 -0.03971055752386121 1106 32-33 0.07450896559080178 1106 34-35 0.079445124973482 1106 36-37 0.05016192303235556 1106 38-39 0.18270191722832863 1106 40-41 0.17107020878312085 1106 42-43 0.1076491450436734 1106 44-45 0.26262219539957243 1106 46-47 0.3971492264108676 1106 48-49 0.3838046278046008 1106 50-51 0.12677272340531687 1106 52-53 0.2193973613241269 1106 54-55 0.2745364249973292 1106 56-57 0.2619715203682844 1106 58-59 0.3009305696704345 1106 60-61 0.18708298507586107 1106 62-63 0.1713893542976237 1106 64-65 0.04877139021402144 1106 66-67 0.13576762833677947 1106 68-69 0.2027929818392451 1106 70-71 0.2940577239415276 1106 72-73 0.13860344166488403 1106 74-75 0.38776056900011113 1106 76-77 0.09886072564438919 1106 78-79 0.056605398647935345 1106 80-81 0.487476389425467 1106 82-83 0.3203014977308847 1106 84-85 0.3050138740603714 1106 86-87 0.366812406287643 1106 88-89 0.15449552015405388 1106 90-91 0.1678032212780174 1106 92-93 0.26679555266467503 1106 94-95 0.38493235691897 1106 96-97 0.42104825336983964 1106 98-99 0.4494948133091299 1106 100-101 0.40193226143381366 1107 1 0.10075345613303455 1107 2 0.020366865465234696 1107 3 0.010446243147654855 1107 4 0.08478442402030595 1107 5 0.05099125600494858 1107 6 0.03320923241875562 1107 7 0.02274983422456245 1107 8 0.0489559273295157 1107 9 0.02133661993073588 1107 10-11 0.08112301522181298 1107 12-13 0.09716612323627061 1107 14-15 0.12190430068438474 1107 16-17 0.1765713209102202 1107 18-19 0.20003358290667705 1107 20-21 0.174840428694651 1107 22-23 0.06029924757655891 1107 24-25 0.13921063772209408 1107 26-27 0.16491980699260722 1107 28-29 0.17592219545923626 1107 30-31 0.06442257287132946 1107 32-33 0.20710116065794182 1107 34-35 0.05186204292267149 1107 36-37 0.16096050960813812 1107 38-39 0.09768265414410848 1107 40-41 0.18814446238453542 1107 42-43 0.11000177053816884 1107 44-45 0.1254010763368072 1107 46-47 0.19626926151180868 1107 48-49 0.5062471105557691 1107 50-51 0.2141670122973025 1107 52-53 0.34739192172213507 1107 54-55 0.21251446131238083 1107 56-57 0.20233240482271952 1107 58-59 0.3392130606137158 1107 60-61 0.26049853032437653 1107 62-63 0.38225997743641216 1107 64-65 0.22032934503933888 1107 66-67 0.22971869040792114 1107 68-69 0.32096980493437144 1107 70-71 0.22941539768439867 1107 72-73 0.2612820166067351 1107 74-75 0.3351393886387193 1107 76-77 0.4569753635735445 1107 78-79 0.30453135482723326 1107 80-81 0.4875407458810592 1107 82-83 0.42107090700973515 1107 84-85 0.4748765162875479 1107 86-87 0.4963444104093 1107 88-89 0.2910967786961045 1107 90-91 0.17456005651434836 1107 92-93 0.21179579089022837 1107 94-95 0.35570409630448196 1107 96-97 0.3664636677720772 1107 98-99 0.39604209539259827 1107 100-101 0.7014301388559794 1108 1 -0.00781737936839022 1108 2 -0.0426786179502443 1108 3 -0.03525130674695731 1108 4 0.019915049529430462 1108 5 -0.09370433692599534 1108 6 0.023319455416086043 1108 7 0.0732996137996551 1108 8 -0.04684931062325859 1108 9 -0.0860702989537927 1108 10-11 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3960.0 13 5649.0 14 7197.0 15 10049.0 16 13478.0 17 18730.0 18 17516.0 19 10544.0 20 8711.0 21 8836.0 22 9385.0 23 10290.0 24 11634.0 25 13990.0 26 17080.0 27 21473.0 28 27275.0 29 34018.0 30 42950.0 31 54469.0 32 69446.0 33 91587.0 34 126414.0 35 183014.0 36 284214.0 37 445802.0 38 352407.0 39 30281.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.29440942440839 19.916348041748474 15.415624677069339 22.373617856773794 2 15.306102418134198 21.009252036636205 40.22471445766328 23.459931087566314 3 19.087429096882847 25.22807815099135 30.518819676196184 25.165673075929618 4 12.93676149644227 17.696243397858904 37.72480855405039 31.64218655164844 5 13.453441105228439 37.58790216223951 34.44845251384816 14.510204218683894 6 30.725729469277784 37.21135290155419 17.72173345289701 14.34118417627102 7 27.445803769561678 33.35676740044051 21.244281812133508 17.953147017864303 8 25.789518814252197 35.11441036485899 20.042863208614378 19.053207612274438 9 25.91676963192846 17.01793661747751 20.965345818646146 36.09994793194788 10-11 24.31658098854916 27.296495530825528 27.58144931009831 20.805474170527003 12-13 25.560681731131563 24.629834479598767 28.234210580009705 21.57527320925997 14-15 22.52338413412069 25.812995111388442 26.973084742821012 24.690536011669856 16-17 21.447801777752066 29.13282415461732 27.460654528474198 21.958719539156416 18-19 21.283920082114353 27.473103365624276 30.321948593812394 20.92102795844898 20-21 22.0311115392771 26.52172329692746 30.55843893206564 20.888726231729805 22-23 21.772219834621946 26.447755288419817 30.467119644945473 21.31290523201276 24-25 21.66033772820468 26.62648380502909 30.559681002904714 21.153497463861513 26-27 21.605510653399552 26.78619950057185 30.407647635568292 21.200642210460302 28-29 21.26537041121904 26.910378808346607 30.54340857772651 21.28084220270785 30-31 21.562808333262286 26.649398066299128 30.493573491923364 21.294220108515223 32-33 21.428973601100047 26.80826730939494 30.403365025512496 21.35939406399251 34-35 21.771388028009493 26.79932546200145 30.246014205177225 21.18327230481183 36-37 21.73735703814684 26.601243792339634 30.189487823621725 21.471911345891805 38-39 21.536297026792678 26.90097979022834 30.304661474766004 21.258061708212978 40-41 21.779827182235618 26.78388635655074 30.23226019423595 21.204026266977692 42-43 21.603312317098055 26.838653686709147 29.91832855065464 21.63970544553816 44-45 21.743274163691915 26.936895960972677 29.730413825896257 21.589416049439155 46-47 21.92156847552227 27.23338201888793 29.21825462932472 21.62679487626508 48-49 21.98512576208193 27.39445141077975 28.989293079824733 21.631129747313587 50-51 21.907284460325926 27.479463341370213 28.876898858005518 21.736353340298344 52-53 22.151869309743656 27.667411377284175 28.523174275198553 21.657545037773616 54-55 21.984987829924748 27.48869257439449 28.65825436903635 21.868065226644408 56-57 21.958786983432923 27.803999912186338 28.50452046639384 21.732692637986897 58-59 22.14348253926438 27.508749092806244 28.611070217442396 21.73669815048698 60-61 22.087417507262668 27.49438512176073 28.89841461046072 21.519782760515888 62-63 21.887157447786656 27.54098487675804 28.954545043654782 21.61731263180052 64-65 21.943607512627157 27.54687643270315 28.722897292215265 21.786618762454427 66-67 21.931162836826896 27.74244137079259 28.454610516517633 21.87178527586288 68-69 21.837112514870245 27.54909440661848 28.687136250441007 21.92665682807027 70-71 22.013601675117243 27.535656550342235 28.572041070023236 21.878700704517286 72-73 21.9859909161542 27.403029532830786 28.61553490544204 21.995444645572967 74-75 21.869723682045674 27.80655237813828 28.275394836792355 22.048329103023686 76-77 21.968869681826494 27.68017554970344 28.403212625693357 21.947742142776704 78-79 21.929986790642793 27.651260571489384 28.321982860381173 22.096769777486646 80-81 22.116843369448713 27.795911515167166 28.09687875305087 21.99036636233325 82-83 21.98262513608416 27.76938206491489 28.285875529646653 21.9621172693543 84-85 21.939136570539507 27.770302101132906 28.174427710407723 22.116133617919857 86-87 21.868091168679825 27.74535003367894 28.279434978986824 22.10712381865441 88-89 21.9309874939014 27.679696553868737 28.117736727008253 22.27157922522161 90-91 21.89380090303738 27.599202649295346 28.243653763008737 22.26334268465854 92-93 21.9800952724335 27.459784020223953 28.262734758941733 22.297385948400812 94-95 21.86015962998366 27.49775364562846 28.208425527057106 22.43366119733077 96-97 21.789043724632574 27.493998339293025 28.244880670584692 22.47207726548971 98-99 21.66132124056587 27.52371866029796 28.023351135349905 22.791608963786267 100-101 21.442658550595276 27.73824430036076 28.06604845674803 22.75304869229593 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 576.0 1 431.0 2 327.0 3 377.0 4 330.5 5 325.0 6 625.5 7 1386.0 8 2342.5 9 2813.5 10 2548.0 11 2270.0 12 2314.5 13 2472.0 14 2680.0 15 2816.5 16 3030.5 17 3261.0 18 3505.5 19 3831.5 20 4221.0 21 4785.5 22 5713.5 23 6908.0 24 8207.0 25 10068.0 26 12831.5 27 16027.5 28 18818.5 29 21672.0 30 25404.5 31 29265.0 32 32968.0 33 36797.0 34 41733.5 35 46557.0 36 51732.5 37 56539.5 38 58890.5 39 60359.5 40 61523.0 41 63345.0 42 65092.0 43 67298.5 44 69825.5 45 71483.0 46 71796.5 47 73024.5 48 76766.5 49 75678.5 50 70949.5 51 67337.5 52 63381.0 53 60663.5 54 57977.0 55 53022.0 56 47804.0 57 42742.0 58 37888.0 59 33430.5 60 28210.5 61 22667.0 62 17613.0 63 13379.0 64 9905.5 65 7301.0 66 5354.5 67 4121.5 68 3038.0 69 1944.0 70 1267.5 71 851.0 72 590.0 73 412.5 74 289.5 75 201.5 76 119.0 77 60.0 78 42.5 79 27.5 80 10.5 81 4.0 82 4.0 83 4.0 84 1.0 85 1.5 86 1.5 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02727374158543086 2 0.0071283642780103395 3 0.009711104958448869 4 1.549644408263117E-4 5 0.005733684310573534 6 0.0 7 0.0 8 1.0330962721754115E-4 9 0.0 10-11 0.0 12-13 1.80791847630697E-4 14-15 0.0 16-17 0.0 18-19 0.004700588038398122 20-21 0.001162233306197338 22-23 0.0 24-25 0.005733684310573534 26-27 0.005888648751399846 28-29 0.007593257600489274 30-31 0.032206776285068454 32-33 7.23167390522788E-4 34-35 0.004364831749941113 36-37 0.016297093693567116 38-39 0.008083978329772595 40-41 0.009788587178862024 42-43 0.006353542073878781 44-45 0.0027635325280692255 46-47 0.0018337458831113552 48-49 0.0015754718150675024 50-51 0.00312511622333062 52-53 0.0027377051212648406 54-55 0.0016529540354806584 56-57 5.423755428920911E-4 58-59 6.715125769140175E-4 60-61 0.0 62-63 2.5827406804385288E-5 64-65 0.0062244050398568545 66-67 5.940303565008615E-4 68-69 7.748222041315585E-4 70-71 0.003280080664156931 72-73 0.009504485704013785 74-75 0.004984689513246361 76-77 0.0034092176981788576 78-79 0.009246211635969932 80-81 1.549644408263117E-4 82-83 0.004416486563549884 84-85 0.0010330962721754116 86-87 0.0 88-89 0.0016787814422850435 90-91 8.781318313490998E-4 92-93 3.099288816526234E-4 94-95 6.973399837184027E-4 96-97 2.8410147484823813E-4 98-99 2.582740680438529E-4 100-101 8.264770177403292E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1935928.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.73714699940661 #Duplication Level Percentage of deduplicated Percentage of total 1 79.39179570794562 48.22031166460351 2 12.395440182682313 15.057273449958544 3 3.634232921277532 6.621988175091491 4 1.5221183237923979 3.6979649753067703 5 0.7885193115418117 2.3946206668492955 6 0.48721157666380577 1.775510469098534 7 0.3133157938220367 1.3320935198623374 8 0.23921256837389154 1.1623271143544522 9 0.17454099807011086 0.9541010031486722 >10 0.9495438637550997 10.511945768793277 >50 0.06963549677891653 2.914529871659928 >100 0.03281853328008534 3.4307547094210045 >500 0.0011048098006533858 0.4436024164145752 >1k 2.54956107843088E-4 0.28492964144850447 >5k 2.54956107843088E-4 1.198046553989092 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9948 0.5138620857800497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7938 0.4100359104264208 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5324 0.2750102276530945 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2118 0.10940489522337608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.1654813608770576E-5 0.0 14-15 0.0 0.0 0.0 1.0330962721754115E-4 0.0 16-17 0.0 0.0 0.0 1.0330962721754115E-4 0.0 18-19 0.0 0.0 0.0 1.0330962721754115E-4 0.0 20-21 0.0 0.0 0.0 1.0330962721754115E-4 0.0 22-23 0.0 0.0 0.0 2.3244666123946756E-4 0.0 24-25 0.0 0.0 0.0 4.648933224789352E-4 0.0 26-27 0.0 0.0 0.0 7.231673905227881E-4 0.0 28-29 0.0 0.0 0.0 0.0015238170014587318 0.0 30-31 0.0 0.0 0.0 0.0030992888165262346 0.0 32-33 0.0 0.0 0.0 0.0048555524792244335 0.0 34-35 0.0 0.0 0.0 0.0062244050398568545 0.0 36-37 0.0 0.0 0.0 0.008471389431838373 0.0 38-39 0.0 0.0 0.0 0.012474637486518093 0.0 40-41 0.0 0.0 0.0 0.015909682591501335 0.0 42-43 0.0 0.0 0.0 0.019757966205354745 0.0 44-45 0.0 0.0 0.0 0.021695021715683643 0.0 46-47 0.0 0.0 0.0 0.023916178700860775 0.0 48-49 0.0 0.0 0.0 0.027996908975953652 0.0 50-51 0.0 0.0 0.0 0.03241339553950354 0.0 52-53 0.0 0.0 0.0 0.04326090639734535 0.0 54-55 0.0 0.0 0.0 0.05018265142092061 0.0 56-57 0.0 0.0 0.0 0.0695273791174052 0.0 58-59 0.0 0.0 0.0 0.0974209784661413 0.0 60-61 0.0 0.0 0.0 0.12038154311523983 0.0 62-63 0.0 0.0 0.0 0.1328820080085623 0.0 64-65 0.0 0.0 0.0 0.15597170969168273 0.0 66-67 0.0 0.0 0.0 0.2099768173196524 0.0 68-69 0.0 0.0 0.0 0.29215962577120635 0.0 70-71 0.0 0.0 0.0 0.35980160419189144 0.0 72-73 0.0 0.0 0.0 0.44244930596592436 0.0 74-75 0.0 0.0 0.0 0.5375974726332797 0.0 76-77 0.0 0.0 0.0 0.5960190668247993 0.0 78-79 0.0 0.0 0.0 0.6732946679835201 0.0 80-81 0.0 0.0 0.0 0.7801943047468707 0.0 82-83 0.0 0.0 0.0 0.8770987350769244 0.0 84-85 0.0 0.0 0.0 1.0090251290337244 0.0 86-87 0.0 0.0 0.0 1.2156185560620023 0.0 88-89 0.0 0.0 0.0 1.4825189779785197 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6640 0.0 39.571648 1 GTATCAA 10535 0.0 34.006653 1 ATCAACG 12675 0.0 27.954365 3 TCAACGC 12700 0.0 27.898617 4 TATCAAC 12955 0.0 27.460876 2 CAACGCA 12920 0.0 27.3868 5 AACGCAG 13220 0.0 26.83509 6 ACGCAGA 14785 0.0 23.898218 7 CGCAGAG 14880 0.0 23.586061 8 GAGTACT 8605 0.0 22.684107 12-13 GCAGAGT 15720 0.0 21.6611 9 GTACATG 7760 0.0 21.124405 1 GTACTTT 9300 0.0 20.785429 14-15 AGAGTAC 13055 0.0 20.426163 10-11 TACATGG 7940 0.0 20.039843 2 AGTACTT 9290 0.0 19.733418 12-13 ACATGGG 7695 0.0 19.442884 3 TAGACCG 175 9.9493336E-5 18.997986 5 CAGAGTA 15520 0.0 18.941435 10-11 GTATAGC 860 0.0 18.784842 1 >>END_MODULE