FastQCFastQC Report
Thu 26 May 2016
SRR936817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994892
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84510.42363195601566406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63780.3197165560842391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42310.212091682156227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91300.035.5449331
GGTATCA56200.035.1711
ATCAACG110750.029.2181633
TCAACGC111600.028.9530454
TATCAAC113600.028.5687922
CAACGCA114050.028.4144085
GTATCGA1205.371363E-627.716891
AACGCAG117000.027.6139836
ACGCAGA131900.024.5647777
CGCAGAG132750.024.2285488
GTACATG81100.024.0209791
TACATGG83550.022.578422
GAGTACT70900.022.14671912-13
GCAGAGT141500.021.9586419
ACATGGG82650.021.8469223
AGAGTAC115150.020.69146310-11
CGACGCA1150.003716486520.6595885
TCGACGC1150.003716486520.6595884
ATCGACG1150.003716486520.6595883
ATACCGT1856.5414188E-620.5458556
Produced by FastQC (version 0.11.5)