##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936817_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994892 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66109293134666 33.0 31.0 34.0 30.0 34.0 2 31.791585709903092 33.0 31.0 34.0 30.0 34.0 3 31.7924589401331 33.0 31.0 34.0 30.0 34.0 4 35.37238858043443 37.0 35.0 37.0 33.0 37.0 5 35.36234543022881 37.0 35.0 37.0 33.0 37.0 6 35.46427024620882 37.0 35.0 37.0 33.0 37.0 7 35.39949982254679 37.0 35.0 37.0 33.0 37.0 8 35.359581370821076 37.0 35.0 37.0 33.0 37.0 9 36.9461193889193 39.0 37.0 39.0 33.0 39.0 10-11 36.81463582990959 39.0 37.0 39.0 33.0 39.0 12-13 36.708215281829794 39.0 37.0 39.0 32.0 39.0 14-15 37.851606753648824 40.0 38.0 41.0 32.5 41.0 16-17 37.765875546144855 40.0 37.0 41.0 32.0 41.0 18-19 37.709713357916115 40.0 37.0 41.0 32.0 41.0 20-21 37.59607587779188 40.0 37.0 41.0 32.0 41.0 22-23 37.46174905709181 40.0 37.0 41.0 32.0 41.0 24-25 37.587476164123174 40.0 37.0 41.0 32.0 41.0 26-27 37.53686690808324 40.0 37.0 41.0 32.0 41.0 28-29 37.44390598588796 40.0 37.0 41.0 32.0 41.0 30-31 37.257699163664 40.0 37.0 41.0 31.0 41.0 32-33 37.05470697160548 39.5 36.0 41.0 31.0 41.0 34-35 36.86443125743148 39.0 36.0 41.0 30.0 41.0 36-37 36.67066136913678 39.0 36.0 41.0 30.0 41.0 38-39 36.5066572526232 39.0 35.0 41.0 30.0 41.0 40-41 36.32273250882754 39.0 35.0 41.0 30.0 41.0 42-43 36.09377149239157 39.0 35.0 40.0 29.5 41.0 44-45 35.82547977534624 39.0 35.0 40.0 28.5 41.0 46-47 35.483815163928675 38.5 35.0 40.0 27.0 41.0 48-49 35.22583428075305 38.5 35.0 40.0 26.0 41.0 50-51 34.52587057344458 38.0 34.0 39.5 25.0 40.5 52-53 34.730449066916904 38.0 34.0 39.5 26.0 40.5 54-55 35.13680966187643 39.0 35.0 40.0 26.0 41.0 56-57 35.034704886279556 38.5 34.5 40.0 26.0 41.0 58-59 34.78296068158076 38.0 34.0 40.0 25.5 41.0 60-61 34.49478092047089 38.0 34.0 40.0 24.5 41.0 62-63 34.154345197634754 38.0 34.0 40.0 23.0 41.0 64-65 33.81982934414495 37.0 33.5 40.0 22.5 41.0 66-67 33.43492204089244 36.5 33.0 39.5 22.0 41.0 68-69 33.02245134072421 36.0 32.5 39.0 21.5 41.0 70-71 32.625315555929845 35.5 32.0 39.0 20.0 40.0 72-73 32.20517702211448 35.0 32.0 38.0 18.5 40.0 74-75 31.749415256565268 35.0 31.0 37.0 18.0 39.5 76-77 31.308796416046583 35.0 31.0 37.0 14.5 39.0 78-79 30.86251586552054 35.0 31.0 36.0 11.0 39.0 80-81 30.403197516457034 34.5 30.0 36.0 7.5 37.5 82-83 29.970642019718362 34.0 30.0 35.5 6.5 37.0 84-85 29.557004589722148 34.0 29.5 35.0 2.0 37.0 86-87 29.197287622588092 34.0 29.0 35.0 2.0 36.0 88-89 28.82228085530445 34.0 29.0 35.0 2.0 36.0 90-91 28.409664031937567 34.0 29.0 35.0 2.0 35.5 92-93 28.014765460987363 34.0 27.5 35.0 2.0 35.0 94-95 27.57322952821506 34.0 27.0 35.0 2.0 35.0 96-97 27.010038889323333 33.5 25.5 35.0 2.0 35.0 98-99 26.310421566681306 33.0 25.0 35.0 2.0 35.0 100-101 23.65765063973388 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34047836826147915 1101 2 0.3760669493674236 1101 3 0.25254712970918547 1101 4 0.28048855201388534 1101 5 0.2980335164691752 1101 6 0.13594173144792876 1101 7 0.12313747209705639 1101 8 0.1363703166368495 1101 9 0.27436669551500614 1101 10-11 0.34384944868768486 1101 12-13 0.3374081980795154 1101 14-15 0.3391692399708788 1101 16-17 0.4598746376566254 1101 18-19 0.4845276710829225 1101 20-21 0.5780334382316994 1101 22-23 0.547991297658541 1101 24-25 0.5428429188115871 1101 26-27 0.43585896472981034 1101 28-29 0.41586536997518664 1101 30-31 0.47294766422720613 1101 32-33 0.6069680808785947 1101 34-35 0.638678636712882 1101 36-37 0.5642636702582067 1101 38-39 0.6268468419156719 1101 40-41 0.4171942945230498 1101 42-43 0.6137350023986698 1101 44-45 0.5436418238650376 1101 46-47 0.6364188308939092 1101 48-49 0.8581186025557273 1101 50-51 0.7973074831741442 1101 52-53 0.7834306319980229 1101 54-55 0.8249446955534978 1101 56-57 0.7011004215642984 1101 58-59 0.6900417403672989 1101 60-61 0.8301807573110551 1101 62-63 0.8212916743054208 1101 64-65 0.9608739354328151 1101 66-67 0.845057175823932 1101 68-69 0.8604089920272031 1101 70-71 0.8733232873589429 1101 72-73 0.7404806072770995 1101 74-75 0.6715663903007645 1101 76-77 0.8467837506173659 1101 78-79 0.9351230949387173 1101 80-81 0.875433592493561 1101 82-83 0.9552795504882994 1101 84-85 0.7523714847689114 1101 86-87 0.7217723340056423 1101 88-89 0.607605103122733 1101 90-91 0.7217044670321613 1101 92-93 0.8055112477751756 1101 94-95 0.8092874440533286 1101 96-97 0.6757528475780035 1101 98-99 0.6054858006287915 1101 100-101 0.8560777646514808 1102 1 0.20230934906629727 1102 2 0.37298564572582293 1102 3 0.14591238030523357 1102 4 0.17496073255487943 1102 5 0.22241265598375293 1102 6 0.2601488336436475 1102 7 0.1707711051794476 1102 8 0.27352696237871044 1102 9 0.4465702886616896 1102 10-11 0.41592678864068233 1102 12-13 0.30228184883522147 1102 14-15 0.2770771082235868 1102 16-17 0.5734269521562112 1102 18-19 0.3931576051495185 1102 20-21 0.5374039225188838 1102 22-23 0.5515085643569435 1102 24-25 0.20701540009773112 1102 26-27 0.3098112126343082 1102 28-29 0.1756756593436677 1102 30-31 0.19584954532508192 1102 32-33 0.4821253370754519 1102 34-35 0.3641252186984829 1102 36-37 0.331607422592775 1102 38-39 0.35877299820496233 1102 40-41 0.29813603875947337 1102 42-43 0.359109204911789 1102 44-45 0.16614018566848898 1102 46-47 0.3295833357103177 1102 48-49 0.2807840924245326 1102 50-51 0.42290187637973986 1102 52-53 0.3429973797029433 1102 54-55 0.34544509789884614 1102 56-57 0.40629793363999767 1102 58-59 0.4917541093382596 1102 60-61 0.28839778046778974 1102 62-63 0.4790500024271225 1102 64-65 0.5082505797659351 1102 66-67 0.40583896377406603 1102 68-69 0.33424288423280046 1102 70-71 0.25070123371147446 1102 72-73 0.1939361881378474 1102 74-75 0.30241517888909897 1102 76-77 0.4823272796876168 1102 78-79 0.33282556429323407 1102 80-81 0.3183654413824719 1102 82-83 0.5044312101361577 1102 84-85 0.37354573552077497 1102 86-87 0.4779303522040337 1102 88-89 0.5324114544161063 1102 90-91 0.44044618266685376 1102 92-93 0.263661035503322 1102 94-95 0.2422249065669071 1102 96-97 0.1854864031795671 1102 98-99 -0.09404376481689525 1102 100-101 0.1256040003426122 1103 1 0.0032326553428845273 1103 2 0.1740510308005483 1103 3 0.10337192903002546 1103 4 0.04981855080109199 1103 5 0.09027154908696389 1103 6 0.04145085198622667 1103 7 -0.03033422668779906 1103 8 0.08696596939204682 1103 9 0.029662088038186596 1103 10-11 0.0856123209008004 1103 12-13 0.2870909484674584 1103 14-15 0.2620933546252928 1103 16-17 0.28515586580936514 1103 18-19 0.2860318386446963 1103 20-21 0.402892570628822 1103 22-23 0.4802751968423351 1103 24-25 0.1594888066531226 1103 26-27 0.35986310228232554 1103 28-29 0.39099648974276136 1103 30-31 0.31140202593853417 1103 32-33 0.5159862151636148 1103 34-35 0.2756640762202167 1103 36-37 0.3743716994528725 1103 38-39 0.4325825220144921 1103 40-41 0.3363527645226867 1103 42-43 0.20951855657946084 1103 44-45 0.3307648299567134 1103 46-47 0.22087699405404493 1103 48-49 0.2755588872339203 1103 50-51 0.1285141948174413 1103 52-53 0.14597135663263572 1103 54-55 0.27225495528468 1103 56-57 0.3280201345847118 1103 58-59 0.3755381825109012 1103 60-61 0.42144098477710656 1103 62-63 0.47455392915563976 1103 64-65 0.33672811278380976 1103 66-67 0.21873992455619629 1103 68-69 0.11235245375463165 1103 70-71 0.2468467800559324 1103 72-73 0.13666999012530567 1103 74-75 0.19786453912268698 1103 76-77 0.11740797632673505 1103 78-79 0.09354921897174506 1103 80-81 0.17754208445170505 1103 82-83 0.13303846727407276 1103 84-85 0.1925332284683101 1103 86-87 0.10863028590059542 1103 88-89 0.1969930632422887 1103 90-91 0.19960244051722142 1103 92-93 0.3436182471348417 1103 94-95 0.23050237616410385 1103 96-97 0.24952502390330267 1103 98-99 0.1673367469138718 1103 100-101 0.6162078558258202 1104 1 0.41382277163962655 1104 2 0.4490590858545609 1104 3 0.5579158913870899 1104 4 0.41552104270044765 1104 5 0.3784509852939735 1104 6 0.3594978205502386 1104 7 0.2961240420531226 1104 8 0.3248253311885989 1104 9 0.35704501618710793 1104 10-11 0.47008156883232033 1104 12-13 0.3950329277614131 1104 14-15 0.6027628663978319 1104 16-17 0.556588388773875 1104 18-19 0.5276503269694288 1104 20-21 0.6481163795447031 1104 22-23 0.5384919598656808 1104 24-25 0.4352649794993795 1104 26-27 0.2793928087136024 1104 28-29 0.3564451714495789 1104 30-31 0.24794522230555316 1104 32-33 0.39046415647418087 1104 34-35 0.331611137797303 1104 36-37 0.3688504918634905 1104 38-39 0.2966414789305887 1104 40-41 0.33676159650945436 1104 42-43 0.49649125450581266 1104 44-45 0.4021982648141247 1104 46-47 0.2752017981490482 1104 48-49 0.3713517173239538 1104 50-51 0.21142137019877083 1104 52-53 0.2619279626888229 1104 54-55 0.2644561431771706 1104 56-57 0.24269249146312433 1104 58-59 0.3694038789235137 1104 60-61 0.40262863409957106 1104 62-63 0.5670940372453472 1104 64-65 0.6133635927130499 1104 66-67 0.28116264163230653 1104 68-69 0.34190143152268604 1104 70-71 0.36162947597612316 1104 72-73 0.31062845644301973 1104 74-75 0.30899479998484836 1104 76-77 0.26096454104377287 1104 78-79 0.348735153935543 1104 80-81 0.3324097687908534 1104 82-83 0.16904243096857385 1104 84-85 0.13592595642633754 1104 86-87 0.17719156206432274 1104 88-89 0.1908526803688524 1104 90-91 0.14053761709008583 1104 92-93 0.1360002937872835 1104 94-95 0.4382017919447172 1104 96-97 0.30987379254391456 1104 98-99 0.2657397184688932 1104 100-101 0.24340445385735165 1105 1 0.18203579328239528 1105 2 0.2501150121010305 1105 3 0.2287311942524184 1105 4 0.2870448460202226 1105 5 0.31447810932188247 1105 6 0.25176480486334185 1105 7 0.1507629518806226 1105 8 0.206320875142616 1105 9 0.28158723617588066 1105 10-11 0.23535822212929958 1105 12-13 0.39203979252282295 1105 14-15 0.28404933447342984 1105 16-17 0.40471523641215157 1105 18-19 0.4362010340739815 1105 20-21 0.35749098209890207 1105 22-23 0.38698329438398815 1105 24-25 0.2576854205440142 1105 26-27 0.15107667605899167 1105 28-29 0.14729608545859207 1105 30-31 0.14366444022527958 1105 32-33 0.24520140020888448 1105 34-35 -0.13164641328514648 1105 36-37 -0.02958361045960345 1105 38-39 0.17209460591781323 1105 40-41 0.19773079038855457 1105 42-43 0.07848684343906598 1105 44-45 -0.07850595485824385 1105 46-47 -0.007026131782616574 1105 48-49 -0.08806305633745382 1105 50-51 -0.07416976548030618 1105 52-53 -0.02035270151484525 1105 54-55 0.03335657156557659 1105 56-57 0.22738125068774906 1105 58-59 0.1985746169861855 1105 60-61 -0.03953893060072744 1105 62-63 0.10017114405706451 1105 64-65 0.14768522834405218 1105 66-67 -0.04142127565554432 1105 68-69 0.23811759240564356 1105 70-71 0.25990227346721184 1105 72-73 0.06974946918920466 1105 74-75 0.05595156718785432 1105 76-77 0.09382605774347397 1105 78-79 0.1698570910824948 1105 80-81 0.24193078650026578 1105 82-83 0.21113120446246825 1105 84-85 0.3249054241824325 1105 86-87 0.24398510118539463 1105 88-89 0.2914219003048899 1105 90-91 0.4355362366929647 1105 92-93 0.3496513355993578 1105 94-95 0.3446738381275303 1105 96-97 0.533135500327802 1105 98-99 0.394679046137945 1105 100-101 0.3524980137619309 1106 1 0.1695472252734902 1106 2 0.30812108463781485 1106 3 0.25675700381705013 1106 4 0.3002683124651355 1106 5 0.3334090160359793 1106 6 0.3356989326398505 1106 7 0.2994186181700016 1106 8 0.3447104209343195 1106 9 0.3942964020447661 1106 10-11 0.3829714277152121 1106 12-13 0.44929856146955416 1106 14-15 0.47344256416811703 1106 16-17 0.3715226801889955 1106 18-19 0.1277509425752683 1106 20-21 0.3597357802756065 1106 22-23 0.2755592383092704 1106 24-25 0.38269173006202095 1106 26-27 0.23784334965542797 1106 28-29 0.3739378220432741 1106 30-31 0.33277534572452794 1106 32-33 0.018064633813331454 1106 34-35 0.2589704993030111 1106 36-37 0.3183925100466283 1106 38-39 0.20681641819960106 1106 40-41 0.2829466058477763 1106 42-43 0.172601004221832 1106 44-45 0.35744537929468834 1106 46-47 0.43475124018978306 1106 48-49 0.280621116140388 1106 50-51 0.057282090972378796 1106 52-53 0.11732472427025442 1106 54-55 0.17840629484508952 1106 56-57 0.36782299394554485 1106 58-59 0.21149715150459514 1106 60-61 0.442647268194861 1106 62-63 0.5534441370100822 1106 64-65 0.4198839306793829 1106 66-67 0.30701330084533396 1106 68-69 0.3112904629326252 1106 70-71 0.2870161972687697 1106 72-73 0.2512915196670562 1106 74-75 0.395047094573723 1106 76-77 0.4480333085132351 1106 78-79 0.3963465437072138 1106 80-81 0.20609293811862273 1106 82-83 0.44081188296451046 1106 84-85 0.37619464051035223 1106 86-87 0.3756910494139838 1106 88-89 0.326758944062572 1106 90-91 0.30755739560812145 1106 92-93 0.04749504178029085 1106 94-95 -6.778437119230318E-4 1106 96-97 -0.12402069082791911 1106 98-99 -0.20794430173080514 1106 100-101 -0.2454292347150151 1107 1 0.17100700021943638 1107 2 0.20612705545964616 1107 3 0.2081052049878167 1107 4 0.20158986591872718 1107 5 0.20756181361298331 1107 6 0.25024685303467464 1107 7 -0.03347853030761172 1107 8 0.13501736797252306 1107 9 0.1749373771345617 1107 10-11 0.12520350983351136 1107 12-13 0.12372585219831933 1107 14-15 0.2779918494460176 1107 16-17 0.2801329259112748 1107 18-19 0.21872299930240757 1107 20-21 0.3127176164983041 1107 22-23 0.23393691228853442 1107 24-25 0.3145844979149075 1107 26-27 0.026510162198491116 1107 28-29 0.11862539380796022 1107 30-31 0.1365706955895547 1107 32-33 0.24910738281783296 1107 34-35 0.30085854422716096 1107 36-37 0.38814843428911416 1107 38-39 0.2517126220914676 1107 40-41 0.2537830625246542 1107 42-43 0.3949767456999851 1107 44-45 0.3591122698310656 1107 46-47 0.5780103426600007 1107 48-49 0.5959143060237864 1107 50-51 0.6148064963816537 1107 52-53 0.7597085683876088 1107 54-55 0.7522138636633642 1107 56-57 0.8896862543262429 1107 58-59 0.8271735918895331 1107 60-61 0.7374654787681507 1107 62-63 0.7944814048174678 1107 64-65 0.7518814478920675 1107 66-67 0.7261564359167565 1107 68-69 0.7832440655739958 1107 70-71 0.7099162271662038 1107 72-73 0.7167137234982661 1107 74-75 0.7248164943284827 1107 76-77 0.6561382225256018 1107 78-79 0.6015764290137042 1107 80-81 0.5525477084932966 1107 82-83 0.5839780433967654 1107 84-85 0.6421337667252054 1107 86-87 0.6112612022375572 1107 88-89 0.6924666900989997 1107 90-91 0.6665298769655514 1107 92-93 0.468543380305956 1107 94-95 0.5268749209345707 1107 96-97 0.2234425764855139 1107 98-99 0.30332854922829355 1107 100-101 0.18642551139506836 1108 1 0.028672510347981017 1108 2 0.1562135252623129 1108 3 0.17986821495944127 1108 4 0.2565422371826145 1108 5 0.2770073462951075 1108 6 0.27216754323468706 1108 7 0.1267957921652254 1108 8 0.3754540675316207 1108 9 0.2791786181439804 1108 10-11 0.17286471270530512 1108 12-13 0.26360758661589045 1108 14-15 0.2925218065249524 1108 16-17 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70-71 -1.3197700031962185 2316 72-73 -2.143376238512026 2316 74-75 -2.2248504020931357 2316 76-77 -2.3005018394219228 2316 78-79 -2.3918247539571382 2316 80-81 -2.4842647066586956 2316 82-83 -2.0973828292939203 2316 84-85 -2.141195641456452 2316 86-87 -2.5536870289164746 2316 88-89 -2.347853700920094 2316 90-91 -2.1642579441313785 2316 92-93 -2.5288779799995424 2316 94-95 -2.2626995474128826 2316 96-97 -2.44339675575371 2316 98-99 -2.243175127056432 2316 100-101 -2.2126196286711917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4340.0 3 1544.0 4 1586.0 5 1736.0 6 2033.0 7 2456.0 8 2977.0 9 3822.0 10 4714.0 11 5950.0 12 7385.0 13 8876.0 14 9920.0 15 11880.0 16 14518.0 17 19192.0 18 17132.0 19 11918.0 20 11840.0 21 12382.0 22 13671.0 23 15431.0 24 17341.0 25 20078.0 26 23370.0 27 27460.0 28 33048.0 29 39359.0 30 48093.0 31 59499.0 32 75117.0 33 97535.0 34 130510.0 35 184841.0 36 271758.0 37 389868.0 38 336470.0 39 55213.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3380582036501 19.34776733581995 14.74475421565565 22.5694202448743 2 14.96152758643509 20.43123795543737 40.869193793879305 23.738040664248228 3 18.945675480695716 25.2422842778798 30.083808727397788 25.728231514026696 4 12.550658440650093 17.16337801487602 38.21698333153671 32.06898021293718 5 13.618784184009971 37.47191926993864 34.44444165803577 14.46485488801563 6 30.672208434111358 36.90925439390513 18.06737881186659 14.351158360116928 7 27.544851637641283 33.11039539230393 21.442100977851936 17.902651992202852 8 25.68891171358044 35.051220145821695 20.2903093470121 18.969558793585772 9 26.052408976745845 17.21951931277963 20.9678025906146 35.76026911985992 10-11 24.19502101318551 27.194401297191924 27.851495791847626 20.75908189777494 12-13 25.492986527161925 24.60483477689647 28.186751813699495 21.715426882242106 14-15 22.57690430452528 25.787894505025093 26.78331174523656 24.85188944521307 16-17 21.647694612812423 29.145276155375893 27.256059179070636 21.95097005274105 18-19 21.509839312125543 27.712792359178763 29.746083076383144 21.03128525231255 20-21 22.313294871585974 26.79760205251871 29.847151528672715 21.041951547222595 22-23 22.03462012015625 26.739528438851057 29.699042880862432 21.526808560130263 24-25 21.825548505465946 26.958384141183373 29.824736415349008 21.391330938001683 26-27 21.7415333114608 27.089769042577 29.703495152493254 21.465202493468944 28-29 21.476974856642713 27.16213973945793 29.831650743091803 21.529234660807553 30-31 21.758646583373935 26.941483548984106 29.747600371348426 21.552269496293533 32-33 21.49912701559642 27.143849683264314 29.70395226582438 21.653071035314888 34-35 21.90375862735323 27.11567376755767 29.53848845337626 21.44207915171284 36-37 21.936601079156166 26.928901414211897 29.468036364875893 21.666461141756045 38-39 21.65847530674051 27.24906905731228 29.572334065120998 21.52012157082621 40-41 21.897498107665083 27.061542240424284 29.556416644526767 21.484543007383866 42-43 21.730027816111892 27.12542113914222 29.30333898611801 21.84121205862788 44-45 21.85429550646227 27.25665664270708 29.118341038865857 21.77070681196479 46-47 21.973751454653048 27.425250947231966 28.7566266135276 21.844370984587382 48-49 22.03832147844 27.552294328524457 28.531322458696273 21.878061734339273 50-51 21.922960085979764 27.685664558611812 28.4881406875975 21.903234667810924 52-53 22.170189056342444 27.835237331139716 28.178415610150275 21.81615800236756 54-55 21.989605695687757 27.6959459205426 28.28025270165439 22.034195682115254 56-57 22.026354427803536 28.021643374573845 28.109417945814364 21.84258425180825 58-59 22.164616028190355 27.7016782827322 28.210333874472305 21.92337181460514 60-61 22.147328190509562 27.620733415095156 28.575381670061713 21.656556724333566 62-63 21.941013299566205 27.71899694185923 28.568238196606792 21.77175156196777 64-65 21.930145782562086 27.72590682678862 28.413250189424843 21.93069720122445 66-67 21.975879984989852 27.88390748153624 28.098681949544545 22.041530583929365 68-69 21.785958249512742 27.795897648009927 28.338465973540895 22.079678128936433 70-71 21.941544571136316 27.70194650861939 28.27466730848301 22.081841611761284 72-73 22.004988656447026 27.628508040761584 28.293453328486734 22.07304997430466 74-75 21.86339925514569 28.00378443747857 28.011731355077302 22.12108495229844 76-77 21.975732701181773 27.799057037804985 28.08685983058773 22.13835043042551 78-79 21.916133048179713 27.813232568581142 28.028501657449144 22.242132725789997 80-81 21.923595609302772 28.019431849355776 27.960102169420086 22.096870371921366 82-83 21.978679120436617 27.97554946384384 28.004108019036693 22.041663396682846 84-85 21.895009066243368 27.984095154316663 27.889509405052376 22.231386374387593 86-87 21.90560347744997 27.916412851925106 28.02277689621547 22.155206774409457 88-89 21.97470782494213 27.813220535954663 27.897096623949857 22.314975015153347 90-91 21.94492684080068 27.7894647644247 28.08015609836323 22.1854522964114 92-93 22.05345434943399 27.78274768393737 27.99781870150058 22.16597926512806 94-95 21.834849828614058 27.70629106983231 28.057686146892063 22.401172954661565 96-97 21.8667020977899 27.74773374513658 27.998701415907906 22.38686274116562 98-99 21.766848450690667 27.769331517498802 27.86550522787854 22.59831480393199 100-101 21.797927314332252 27.749871557277427 27.82920640827008 22.622994720120236 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 387.0 1 296.0 2 256.0 3 332.5 4 319.5 5 328.5 6 559.0 7 1218.5 8 2131.5 9 2612.5 10 2378.0 11 2016.5 12 2079.5 13 2267.0 14 2398.5 15 2551.5 16 2690.0 17 2895.5 18 3166.0 19 3429.5 20 3786.5 21 4438.5 22 5382.0 23 6634.5 24 8069.0 25 9920.0 26 12773.0 27 16033.0 28 18749.5 29 21952.5 30 26057.5 31 29887.0 32 33781.0 33 38131.0 34 43509.5 35 48064.0 36 53406.5 37 58665.0 38 60841.5 39 62339.0 40 63218.5 41 65092.0 42 67299.0 43 69607.5 44 72228.0 45 74208.5 46 74543.5 47 75083.0 48 78832.5 49 78253.5 50 73419.0 51 69523.5 52 65559.5 53 63060.5 54 60207.5 55 54964.0 56 49736.0 57 44741.0 58 39743.0 59 34734.5 60 29380.0 61 23824.0 62 18533.5 63 14064.5 64 10412.0 65 7822.0 66 5865.5 67 4420.5 68 3166.5 69 2166.5 70 1514.0 71 1032.0 72 702.5 73 460.0 74 293.0 75 206.5 76 149.5 77 86.5 78 59.5 79 36.0 80 16.5 81 13.0 82 13.0 83 13.0 84 9.0 85 6.0 86 6.5 87 7.0 88 5.5 89 4.5 90 3.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024111580977817345 2 0.010827653827876395 3 0.011579574232590035 4 0.020402106981230062 5 0.05448916532824835 6 0.04125536620528831 7 0.01584045652596732 8 0.047621625631863784 9 0.027019006542710083 10-11 0.021555051601790977 12-13 0.03481391473824147 14-15 0.0176200014837896 16-17 0.01681795305209505 18-19 0.01714378522747096 20-21 0.013509503271355042 22-23 0.007819972209021841 24-25 0.001980057065745915 26-27 2.506401349045462E-5 28-29 0.002556529376026371 30-31 0.0 32-33 5.514082967900016E-4 34-35 7.519204047136387E-5 36-37 0.0 38-39 2.757041483950008E-4 40-41 1.0025605396181848E-4 42-43 2.506401349045462E-4 44-45 2.506401349045462E-4 46-47 2.506401349045462E-5 48-49 2.757041483950008E-4 50-51 2.0051210792363695E-4 52-53 5.26344283299547E-4 54-55 0.0024813373355550075 56-57 4.511522428281832E-4 58-59 0.0012782646880131856 60-61 0.0036844099830968297 62-63 0.0026317214164977352 64-65 0.0018798010117840964 66-67 0.012105918515889581 68-69 0.015263984215686864 70-71 0.0276706708934619 72-73 0.018396985901993693 74-75 0.02045223500821097 76-77 0.032307513389196005 78-79 0.020627683102644154 80-81 0.04757149760488287 82-83 0.03727018806030602 84-85 0.020326914940758698 86-87 0.016391864822757323 88-89 0.014186231635597317 90-91 0.025490101719792352 92-93 0.033435393996266466 94-95 0.013584695311826404 96-97 0.020652747116134608 98-99 0.029274767756851 100-101 0.04072902192198876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1994892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.652893868923634 #Duplication Level Percentage of deduplicated Percentage of total 1 80.46580197860193 50.414153514431725 2 11.92948039884457 14.948329386804271 3 3.3658368926668008 6.326382648490823 4 1.3933634938431756 3.4919302040235642 5 0.7278214866034882 2.2800061177845286 6 0.45324341869253965 1.7038207084879082 7 0.318380176213844 1.396320757320662 8 0.2104880749000409 1.0550149613909048 9 0.1586789272380921 0.8947524588734556 >10 0.8811340735213853 10.08764096243554 >50 0.06654571966889172 2.8734376017117254 >100 0.028104965940390178 3.0992231147848033 >500 5.601966323948273E-4 0.20381875247247344 >1k 4.0014045171058414E-4 0.4816440187578229 >5k 1.6005618068423366E-4 0.7435247922298309 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8451 0.42363195601566406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6378 0.3197165560842391 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4231 0.212091682156227 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.519204047136387E-5 0.0 14-15 0.0 0.0 0.0 1.5038408094272772E-4 0.0 16-17 0.0 0.0 0.0 1.5038408094272772E-4 0.0 18-19 0.0 0.0 0.0 1.5038408094272772E-4 0.0 20-21 0.0 0.0 0.0 1.7544809443318233E-4 0.0 22-23 0.0 2.5064013490454623E-5 0.0 2.2557612141409157E-4 0.0 24-25 0.0 5.0128026980909245E-5 0.0 2.757041483950008E-4 0.0 26-27 0.0 5.0128026980909245E-5 0.0 3.0076816188545543E-4 0.0 28-29 0.0 5.0128026980909245E-5 0.0 0.0011028165935800032 0.0 30-31 0.0 5.0128026980909245E-5 0.0 0.0027570414839500085 0.0 32-33 0.0 5.0128026980909245E-5 0.0 0.005113058752052742 0.0 34-35 0.0 5.0128026980909245E-5 0.0 0.006391323440065929 0.0 36-37 0.0 5.0128026980909245E-5 0.0 0.008697212681187753 0.0 38-39 0.0 5.0128026980909245E-5 0.0 0.012281366610322765 0.0 40-41 0.0 5.0128026980909245E-5 0.0 0.01599084060691005 2.5064013490454623E-5 42-43 0.0 5.0128026980909245E-5 0.0 0.01947473848208324 5.0128026980909245E-5 44-45 0.0 5.0128026980909245E-5 0.0 0.021103899358962792 5.0128026980909245E-5 46-47 0.0 5.0128026980909245E-5 0.0 0.0238609408429128 5.0128026980909245E-5 48-49 0.0 5.0128026980909245E-5 0.0 0.02754535082600963 5.0128026980909245E-5 50-51 0.0 5.0128026980909245E-5 0.0 0.031781169105896456 5.0128026980909245E-5 52-53 0.0 5.0128026980909245E-5 0.0 0.041681454434626036 5.0128026980909245E-5 54-55 0.0 5.0128026980909245E-5 0.0 0.048799634265915146 5.0128026980909245E-5 56-57 0.0 5.0128026980909245E-5 0.0 0.06769790043771792 5.0128026980909245E-5 58-59 0.0 5.0128026980909245E-5 0.0 0.09524325126372757 5.0128026980909245E-5 60-61 0.0 5.0128026980909245E-5 0.0 0.11622183055523808 5.0128026980909245E-5 62-63 0.0 5.0128026980909245E-5 0.0 0.1279768528822613 5.0128026980909245E-5 64-65 0.0 5.0128026980909245E-5 0.0 0.15113600134744137 5.0128026980909245E-5 66-67 0.0 5.0128026980909245E-5 0.0 0.20464767014956198 5.0128026980909245E-5 68-69 0.0 5.0128026980909245E-5 0.0 0.2837998247524177 5.0128026980909245E-5 70-71 0.0 1.0025605396181849E-4 0.0 0.35129721308221196 5.0128026980909245E-5 72-73 0.0 1.0025605396181849E-4 0.0 0.43222891264288993 5.0128026980909245E-5 74-75 0.0 1.253200674522731E-4 0.0 0.5232363456267306 5.0128026980909245E-5 76-77 0.0 1.5038408094272772E-4 0.0 0.5809838327087381 5.0128026980909245E-5 78-79 0.0 1.5038408094272772E-4 0.0 0.6551983766539743 5.0128026980909245E-5 80-81 0.0 1.5038408094272772E-4 0.0 0.7637004910541523 5.0128026980909245E-5 82-83 0.0 1.5038408094272772E-4 0.0 0.8553595883887448 5.0128026980909245E-5 84-85 0.0 1.5038408094272772E-4 0.0 0.9894019325356962 5.0128026980909245E-5 86-87 0.0 1.5038408094272772E-4 0.0 1.1853774540175608 5.0128026980909245E-5 88-89 0.0 1.5038408094272772E-4 0.0 1.4428099365780203 5.0128026980909245E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9130 0.0 35.544933 1 GGTATCA 5620 0.0 35.171 1 ATCAACG 11075 0.0 29.218163 3 TCAACGC 11160 0.0 28.953045 4 TATCAAC 11360 0.0 28.568792 2 CAACGCA 11405 0.0 28.414408 5 GTATCGA 120 5.371363E-6 27.71689 1 AACGCAG 11700 0.0 27.613983 6 ACGCAGA 13190 0.0 24.564777 7 CGCAGAG 13275 0.0 24.228548 8 GTACATG 8110 0.0 24.020979 1 TACATGG 8355 0.0 22.57842 2 GAGTACT 7090 0.0 22.146719 12-13 GCAGAGT 14150 0.0 21.958641 9 ACATGGG 8265 0.0 21.846922 3 AGAGTAC 11515 0.0 20.691463 10-11 CGACGCA 115 0.0037164865 20.659588 5 TCGACGC 115 0.0037164865 20.659588 4 ATCGACG 115 0.0037164865 20.659588 3 ATACCGT 185 6.5414188E-6 20.545855 6 >>END_MODULE