FastQCFastQC Report
Thu 26 May 2016
SRR936817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994892
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99780.5001774532155124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79050.3962620532840876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52890.265127134702029No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20380.10216091898709304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64600.038.173691
GTATCAA103000.034.644321
ATCAACG128000.027.569133
TATCAAC129000.027.5033742
TCAACGC129450.027.260324
CAACGCA132100.026.6828615
AACGCAG135500.026.0133296
GTACATG79650.023.4442371
ACGCAGA152100.022.9556437
CGCAGAG152100.022.768258
TACATGG82500.022.2798422
GAGTACT88700.022.0070812-13
ACATGGG80250.021.7202723
GCAGAGT162050.021.04789
GTACTTT93050.020.80040414-15
GTATAGG7750.020.2321281
AGAGTAC133650.020.17103610-11
AGTACTT93500.020.14075912-13
CATGGGG37500.019.1244934
CAGAGTA157050.018.01254810-11
Produced by FastQC (version 0.11.5)