##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936817_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994892 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.244682920178136 33.0 31.0 34.0 31.0 34.0 2 32.37749562382324 34.0 31.0 34.0 31.0 34.0 3 32.480802970787394 34.0 31.0 34.0 31.0 34.0 4 36.116617841968385 37.0 35.0 37.0 35.0 37.0 5 36.03135457959629 37.0 35.0 37.0 35.0 37.0 6 36.070218838914585 37.0 35.0 37.0 35.0 37.0 7 36.02656635045907 37.0 35.0 37.0 35.0 37.0 8 35.98972425574918 37.0 35.0 37.0 35.0 37.0 9 37.6862301317565 39.0 37.0 39.0 35.0 39.0 10-11 37.606018020023136 39.0 37.0 39.0 35.0 39.0 12-13 37.5134265413867 39.0 37.0 39.0 35.0 39.0 14-15 38.73742914403387 40.0 38.0 41.0 35.0 41.0 16-17 38.646720975371096 40.0 38.0 41.0 34.0 41.0 18-19 38.81052257465567 40.0 38.0 41.0 35.0 41.0 20-21 38.84215686864251 40.0 38.0 41.0 35.0 41.0 22-23 38.768078171650394 40.0 38.0 41.0 35.0 41.0 24-25 38.667723365475425 40.0 38.0 41.0 34.0 41.0 26-27 38.497747246467476 40.0 38.0 41.0 34.0 41.0 28-29 38.417788030630234 40.0 38.0 41.0 34.0 41.0 30-31 38.40062469547223 40.0 38.0 41.0 34.0 41.0 32-33 38.23440667464705 40.0 38.0 41.0 34.0 41.0 34-35 38.09479335222157 40.0 37.5 41.0 33.5 41.0 36-37 37.939413261469795 40.0 37.0 41.0 33.0 41.0 38-39 37.78938458823836 40.0 37.0 41.0 33.0 41.0 40-41 37.61767529269755 40.0 37.0 41.0 33.0 41.0 42-43 37.37008569887493 40.0 37.0 41.0 32.5 41.0 44-45 37.10545483164001 40.0 36.5 41.0 31.5 41.0 46-47 36.75578728071494 39.5 36.0 41.0 31.0 41.0 48-49 36.393509022042295 39.0 36.0 41.0 30.0 41.0 50-51 36.10736546138838 39.0 35.5 40.5 29.5 41.0 52-53 35.8726447346523 39.0 35.0 40.0 29.0 41.0 54-55 35.59399381019123 39.0 35.0 40.0 28.0 41.0 56-57 35.32053890636686 38.0 35.0 40.0 28.0 41.0 58-59 34.97587087421274 38.0 34.0 40.0 27.0 41.0 60-61 34.64713553415423 38.0 34.0 40.0 26.0 41.0 62-63 34.284590343737904 37.0 34.0 40.0 26.0 41.0 64-65 33.90316092299734 37.0 33.0 39.0 25.5 40.5 66-67 33.512911475909476 36.0 33.0 39.0 25.0 40.0 68-69 33.20216583153373 36.0 32.5 39.0 24.0 40.0 70-71 32.73096588687508 35.0 32.0 38.5 22.5 40.0 72-73 32.22107688035242 35.0 31.5 37.5 22.0 39.5 74-75 31.69907769443158 35.0 31.0 37.0 20.0 39.0 76-77 30.297315343387012 33.5 29.5 35.5 19.5 38.0 78-79 31.039769571485575 34.0 31.0 36.0 20.0 38.0 80-81 30.804156315229093 34.0 31.0 36.0 18.0 37.5 82-83 30.465391860812517 34.0 30.5 35.0 17.5 37.0 84-85 30.09289751024116 34.0 30.0 35.0 13.5 36.5 86-87 29.73865903517584 34.0 30.0 35.0 8.5 36.0 88-89 29.332578405247002 34.0 29.0 35.0 4.5 36.0 90-91 28.864804460592353 34.0 29.0 35.0 2.0 35.0 92-93 28.36812042957714 33.5 29.0 35.0 2.0 35.0 94-95 27.783768996015823 33.0 27.5 35.0 2.0 35.0 96-97 27.10700554215466 33.0 26.5 34.5 2.0 35.0 98-99 26.259639619588427 32.5 25.0 34.0 2.0 35.0 100-101 22.6094041682457 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21885465872497178 1101 2 0.13369764764890135 1101 3 0.19401585747274197 1101 4 0.19303137465195164 1101 5 0.21506913590018684 1101 6 0.19534893139864806 1101 7 0.24135833332425705 1101 8 0.22747663836405962 1101 9 0.17140404084297955 1101 10-11 0.25638663439999476 1101 12-13 0.3163218376761421 1101 14-15 0.3473810514582567 1101 16-17 0.3839101335026598 1101 18-19 0.324697462326327 1101 20-21 0.2827750076646822 1101 22-23 0.3023762601650688 1101 24-25 0.2944875557808828 1101 26-27 0.17358758307042876 1101 28-29 0.26266430726737156 1101 30-31 0.298934751769373 1101 32-33 0.3032931643353365 1101 34-35 0.3788930683205436 1101 36-37 0.4253655300790413 1101 38-39 0.40270096970882463 1101 40-41 0.4830055368069779 1101 42-43 0.42631372488371255 1101 44-45 0.3876726915657187 1101 46-47 0.2468894644391213 1101 48-49 0.3956502565043749 1101 50-51 0.245449561640676 1101 52-53 0.3054869485452514 1101 54-55 0.31377477205484894 1101 56-57 0.35364907269344314 1101 58-59 0.29277019265349224 1101 60-61 0.37418517369178517 1101 62-63 0.22598457718425635 1101 64-65 0.3722542976472809 1101 66-67 0.1737367373075358 1101 68-69 0.2277992827011417 1101 70-71 0.0880695044497628 1101 72-73 0.13119965967376856 1101 74-75 0.21037510893090428 1101 76-77 0.16253038249673324 1101 78-79 0.2574163354418211 1101 80-81 0.3954229619760987 1101 82-83 0.3710139250626341 1101 84-85 0.40600663120426717 1101 86-87 0.14567783987887495 1101 88-89 0.07665445466064114 1101 90-91 0.2948745968510309 1101 92-93 0.5308324115583325 1101 94-95 0.3071054602142702 1101 96-97 0.1829437645634897 1101 98-99 0.42320636280599544 1101 100-101 0.9939988380961005 1102 1 0.30963422683873176 1102 2 0.2865084087326082 1102 3 0.24458479726607862 1102 4 0.16846141458766084 1102 5 0.2276684148802559 1102 6 0.21700983858600864 1102 7 0.19482586715007955 1102 8 0.15306131978552173 1102 9 0.27486869315043805 1102 10-11 0.2695439674373503 1102 12-13 0.2828798960023491 1102 14-15 0.3140314250951306 1102 16-17 0.3662741738609938 1102 18-19 0.3548674932546305 1102 20-21 0.24850521643602264 1102 22-23 0.2430238120484134 1102 24-25 0.29400175312860455 1102 26-27 0.1883776697934536 1102 28-29 0.22901094752367612 1102 30-31 0.33106731015212887 1102 32-33 0.3118517007801458 1102 34-35 0.23363167191539702 1102 36-37 0.1120815169563869 1102 38-39 0.2006296916166903 1102 40-41 0.2461069470434154 1102 42-43 0.3728641205055112 1102 44-45 0.2682905317929922 1102 46-47 0.25767050532893165 1102 48-49 0.3765547544478167 1102 50-51 0.2776018637604025 1102 52-53 0.27401884695359513 1102 54-55 0.33486396465340107 1102 56-57 0.2593035722377337 1102 58-59 0.4296913009494858 1102 60-61 0.4183414544413182 1102 62-63 0.4400715734254774 1102 64-65 0.3211334937250001 1102 66-67 0.3555716455151625 1102 68-69 0.23371664075433785 1102 70-71 0.20974129737317782 1102 72-73 0.266810530895512 1102 74-75 0.3028705678714019 1102 76-77 0.2897777000165753 1102 78-79 0.2682078351760815 1102 80-81 0.5670973162491784 1102 82-83 0.4050893738858008 1102 84-85 0.2890510300661724 1102 86-87 0.3144675808196986 1102 88-89 0.31469572654150113 1102 90-91 0.36808405335397865 1102 92-93 0.3732996956525625 1102 94-95 0.23355918623096628 1102 96-97 0.3344411936087681 1102 98-99 0.3298098423995093 1102 100-101 0.5868472296752394 1103 1 0.2117192846792193 1103 2 0.15121932985622522 1103 3 0.10769825851151893 1103 4 0.056382245810404186 1103 5 0.03048445784159526 1103 6 0.0838585308433295 1103 7 0.009289900623024039 1103 8 0.04569834346120416 1103 9 0.26742911782190504 1103 10-11 0.17311673443224862 1103 12-13 0.1945023535125472 1103 14-15 0.29745309437688405 1103 16-17 0.228217853001766 1103 18-19 0.13636162684164788 1103 20-21 0.11463095411325952 1103 22-23 0.18901245398101452 1103 24-25 0.064014684358348 1103 26-27 0.10692760122499578 1103 28-29 0.15316331365044533 1103 30-31 0.08163377214296474 1103 32-33 0.09771959632545446 1103 34-35 0.2251981627338111 1103 36-37 0.016303934107973816 1103 38-39 -0.04293146198078546 1103 40-41 0.07698046396726 1103 42-43 0.27888640834480327 1103 44-45 0.2226437026211272 1103 46-47 0.11634245979782776 1103 48-49 0.036135189579589166 1103 50-51 0.14098815748052118 1103 52-53 0.23541315474156477 1103 54-55 -0.03538761777495836 1103 56-57 0.10278998003271766 1103 58-59 -0.04198951182360133 1103 60-61 0.05434648691353061 1103 62-63 0.009940778566665642 1103 64-65 -0.1029727482701972 1103 66-67 -0.0982813667612703 1103 68-69 0.01624321360781522 1103 70-71 -0.05846167351507603 1103 72-73 0.09757676803120319 1103 74-75 0.20757214062192375 1103 76-77 0.08589977247264713 1103 78-79 0.1467650558676965 1103 80-81 0.06091709444152471 1103 82-83 0.093739337027678 1103 84-85 0.14683859549448997 1103 86-87 -8.155678111982922E-4 1103 88-89 0.03332905205020609 1103 90-91 0.0490096658371435 1103 92-93 -0.10576818506713792 1103 94-95 -0.3063293333903232 1103 96-97 -0.2557716363126161 1103 98-99 -0.5620875478806546 1103 100-101 -0.15158570295470142 1104 1 0.18123835235991237 1104 2 0.2082624867078806 1104 3 0.22876298467007672 1104 4 0.10918996407197312 1104 5 0.12295039864150681 1104 6 0.0676718238102012 1104 7 0.11009175845283892 1104 8 0.122090651948497 1104 9 0.18834246975785618 1104 10-11 0.24028129462629266 1104 12-13 0.21091369016517092 1104 14-15 0.24459978146994388 1104 16-17 0.22013270652136896 1104 18-19 0.19685052302567385 1104 20-21 0.10966387344721085 1104 22-23 0.14894144791995956 1104 24-25 0.2529294102129427 1104 26-27 0.12825039307631414 1104 28-29 0.042945553624178956 1104 30-31 0.1227431202611271 1104 32-33 0.19341120630278397 1104 34-35 0.21616403970121922 1104 36-37 0.134662162117678 1104 38-39 0.15265953581243252 1104 40-41 0.22678136734917587 1104 42-43 0.2877715888692052 1104 44-45 0.17694388560763485 1104 46-47 0.2147585018178546 1104 48-49 0.1456352898877995 1104 50-51 0.26127899052509207 1104 52-53 0.2941636790799862 1104 54-55 0.16628448985272115 1104 56-57 0.22610556185078678 1104 58-59 0.21383657196111727 1104 60-61 0.21443171850393128 1104 62-63 0.2605821432447968 1104 64-65 0.23041225899454787 1104 66-67 0.23200350858297014 1104 68-69 0.13143443755047457 1104 70-71 0.022300729748288006 1104 72-73 0.26926097343811506 1104 74-75 0.2705027820473944 1104 76-77 0.18367353792163144 1104 78-79 0.21642303523459816 1104 80-81 0.5028042872096066 1104 82-83 0.46018775732519757 1104 84-85 0.3943798282521165 1104 86-87 0.3127193546475091 1104 88-89 0.31651344287534755 1104 90-91 0.4205710696658791 1104 92-93 0.28971034593417855 1104 94-95 0.36013002962208773 1104 96-97 0.28902388690113767 1104 98-99 0.05355719080123578 1104 100-101 0.0990957850113503 1105 1 0.18717188856508926 1105 2 0.1931172037885176 1105 3 0.16051987347211139 1105 4 0.12451702512895224 1105 5 0.1657209952522436 1105 6 0.1619427104594351 1105 7 0.1692211466422222 1105 8 0.04112493232731396 1105 9 0.22279159824645944 1105 10-11 0.16668999764238634 1105 12-13 0.18133255538567994 1105 14-15 0.14095832488731475 1105 16-17 0.004596569585977761 1105 18-19 0.13489705946970787 1105 20-21 0.2331988688407236 1105 22-23 0.23787252422645366 1105 24-25 0.23880034773523562 1105 26-27 0.2641531262626131 1105 28-29 0.1875308295950333 1105 30-31 0.20202475539579723 1105 32-33 0.1538071476250522 1105 34-35 0.2605045666096615 1105 36-37 0.22982208655256642 1105 38-39 0.25716273243992305 1105 40-41 0.09412861632628022 1105 42-43 0.2515629969052853 1105 44-45 0.28774665632833063 1105 46-47 0.28871366527858555 1105 48-49 0.3338268455798499 1105 50-51 0.31224046038435205 1105 52-53 0.41993305659843827 1105 54-55 0.5202980332545835 1105 56-57 0.4953059551864172 1105 58-59 0.38218856392069256 1105 60-61 0.44985363921020394 1105 62-63 0.4812114802509271 1105 64-65 0.3088420805355696 1105 66-67 0.43756294165852694 1105 68-69 0.6006211820157006 1105 70-71 0.3497054901020391 1105 72-73 0.5639348119657797 1105 74-75 0.6651201638838486 1105 76-77 0.4803311156814196 1105 78-79 0.5770572113856822 1105 80-81 0.5704896559307393 1105 82-83 0.6719751079243537 1105 84-85 0.6648473951238252 1105 86-87 0.523670091531411 1105 88-89 0.6105094909069635 1105 90-91 0.4642521079726727 1105 92-93 0.46200748184280727 1105 94-95 0.20319035452724066 1105 96-97 0.40385245398688596 1105 98-99 0.24276465673334613 1105 100-101 0.6619652738921751 1106 1 0.09985521749245407 1106 2 0.08175399713319109 1106 3 0.07761494571715843 1106 4 0.07349163220639099 1106 5 0.07835620073720406 1106 6 0.08622763888170226 1106 7 0.08530926259020077 1106 8 0.11729386143741749 1106 9 0.16449657117328087 1106 10-11 0.10005079342018774 1106 12-13 0.157672825338949 1106 14-15 0.2071991739473802 1106 16-17 0.12624728493560156 1106 18-19 -0.07480629479839251 1106 20-21 0.12369975046218684 1106 22-23 0.03851645866222242 1106 24-25 0.07633827970195739 1106 26-27 0.1518601747000332 1106 28-29 0.1733009415579616 1106 30-31 0.13363343010613704 1106 32-33 0.09646881458687773 1106 34-35 0.057038438241129086 1106 36-37 0.11246301979658568 1106 38-39 0.17493174676059908 1106 40-41 0.3038902534301826 1106 42-43 0.07703060704808706 1106 44-45 -0.005423814442984565 1106 46-47 0.03489640688606954 1106 48-49 -0.00528333272071535 1106 50-51 -0.14003156496665525 1106 52-53 -0.012579055537315753 1106 54-55 -0.210428528062387 1106 56-57 0.03997445524929333 1106 58-59 0.04632179869721398 1106 60-61 0.05778928041550557 1106 62-63 0.2420873782343591 1106 64-65 0.12375386705740965 1106 66-67 0.21980072878437085 1106 68-69 0.21604344062637892 1106 70-71 0.009046378601944127 1106 72-73 -0.11545055970547224 1106 74-75 0.05512997251082297 1106 76-77 0.053928969675322236 1106 78-79 0.2621377251130532 1106 80-81 0.23047325973920607 1106 82-83 0.16333523895699997 1106 84-85 0.10816118820072163 1106 86-87 0.009830538082734819 1106 88-89 0.0810746269487268 1106 90-91 0.13399953048109836 1106 92-93 0.1584906848364689 1106 94-95 0.08369492775144494 1106 96-97 0.16698630388794555 1106 98-99 -0.05295336213788815 1106 100-101 -0.01866478388162207 1107 1 0.07348199346531459 1107 2 0.03718688171608164 1107 3 0.07163112499780055 1107 4 0.03699942503880749 1107 5 0.03656998042340831 1107 6 0.05648791991762181 1107 7 0.0507703547566436 1107 8 0.1238491998634288 1107 9 0.05030850328442682 1107 10-11 0.06370590696545975 1107 12-13 0.04706237596744245 1107 14-15 0.08480653391688264 1107 16-17 0.06652108481340946 1107 18-19 0.08551265454494938 1107 20-21 0.07088365004145913 1107 22-23 0.09501666014495669 1107 24-25 0.1327061091912256 1107 26-27 0.22183729665992757 1107 28-29 0.13744210513161903 1107 30-31 0.10944770201684406 1107 32-33 0.1533008126457034 1107 34-35 0.10829456396636061 1107 36-37 0.1317918222958383 1107 38-39 0.20266032134604473 1107 40-41 0.28596923033116184 1107 42-43 0.29415274025200233 1107 44-45 0.1568368435606473 1107 46-47 0.14467947713639262 1107 48-49 0.14248530009974303 1107 50-51 0.18353996573888054 1107 52-53 0.34198853650563876 1107 54-55 0.31224553785930453 1107 56-57 0.23427563511488358 1107 58-59 0.31190573034040625 1107 60-61 0.33557147540746257 1107 62-63 0.3726531764309797 1107 64-65 0.28075344242711964 1107 66-67 0.3824913249566322 1107 68-69 0.38228359938611334 1107 70-71 0.2786496742529536 1107 72-73 0.287074257335199 1107 74-75 0.2873556201803389 1107 76-77 0.3298938120204937 1107 78-79 0.5404734560187698 1107 80-81 0.6247196276336915 1107 82-83 0.404298088139587 1107 84-85 0.4534027291753766 1107 86-87 0.4138971313779116 1107 88-89 0.30347777441242485 1107 90-91 0.45035169631222516 1107 92-93 0.5842409372315167 1107 94-95 0.5657209341902281 1107 96-97 0.7650347377387803 1107 98-99 0.5282022605304384 1107 100-101 0.7124488090539138 1108 1 0.0793900779048613 1108 2 0.020892680928653817 1108 3 0.05086322120330067 1108 4 0.07753850311270583 1108 5 0.1218378636556281 1108 6 0.10669321805987408 1108 7 0.09316826303155068 1108 8 0.1181229292767938 1108 9 0.12198948057717729 1108 10-11 0.13149163068199243 1108 12-13 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-1.0882572646247795 2316 52-53 -0.9895315822599997 2316 54-55 -1.150107900881821 2316 56-57 -1.152238201353022 2316 58-59 -1.020411296967552 2316 60-61 -0.9553916335099473 2316 62-63 -1.2082323624471343 2316 64-65 -1.1164078714423908 2316 66-67 -1.1218454949800147 2316 68-69 -1.3789051173852194 2316 70-71 -1.1971534302748665 2316 72-73 -0.9233467120991676 2316 74-75 -0.8759002746371038 2316 76-77 -0.9126397480263115 2316 78-79 -1.1020905599169346 2316 80-81 -1.0772770209855018 2316 82-83 -0.9867206305182847 2316 84-85 -1.020242692761986 2316 86-87 -1.1066538119531764 2316 88-89 -1.281748406801345 2316 90-91 -1.1389094586194695 2316 92-93 -1.2505366053111686 2316 94-95 -0.9102032540740232 2316 96-97 -0.8058362390321321 2316 98-99 -0.9841979717727831 2316 100-101 -1.088759438943331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 12.0 5 29.0 6 79.0 7 168.0 8 440.0 9 896.0 10 1570.0 11 2607.0 12 4024.0 13 5887.0 14 7620.0 15 10437.0 16 14147.0 17 19594.0 18 18277.0 19 11234.0 20 9457.0 21 9793.0 22 10310.0 23 11551.0 24 13320.0 25 15824.0 26 19187.0 27 23999.0 28 30178.0 29 37620.0 30 47449.0 31 59237.0 32 76694.0 33 100480.0 34 136959.0 35 196355.0 36 302145.0 37 455649.0 38 320319.0 39 21338.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.33664007766102 19.932728545440323 15.343968935590691 22.386662441307962 2 15.318324028339584 21.039278486590433 40.15892264264162 23.483474842428365 3 19.115470912711064 25.179708976726562 30.438289391104885 25.266530719457492 4 13.010175564709003 17.756382974990437 37.610439139856744 31.623002320443817 5 13.530657298740984 37.62103412114541 34.33133222249626 14.516976357617356 6 30.697952570865993 37.193291666917304 17.719254977211797 14.389500785004902 7 27.52825716880914 33.3205506864532 21.173527188439273 17.977664956298387 8 25.920400703396474 35.18130304798455 19.969000828115004 18.929295420503966 9 25.956041730579898 17.039218163188785 20.885591801460933 36.119148304770384 10-11 24.335968745041527 27.28058449138626 27.640615102636172 20.74283166093604 12-13 25.502708918578048 24.621533396294133 28.239949831870597 21.63580785325722 14-15 22.500867214866773 25.77495423311137 26.963865713031083 24.760312838990785 16-17 21.49522380158926 29.12187727455923 27.41917858209868 21.963720341752836 18-19 21.271807966827154 27.506755403633644 30.24260301786895 20.97883361167025 20-21 22.12610900111511 26.543390432306474 30.46329596998701 20.867204596591407 22-23 21.825979178859537 26.5484704971182 30.278655385938023 21.34689493808424 24-25 21.670390359858413 26.720324947691577 30.414614624701606 21.194670067748405 26-27 21.618773641444864 26.83412243633431 30.289874725251035 21.257229196969785 28-29 21.318591969313527 26.940694089961553 30.43791235136052 21.302801589364403 30-31 21.54123755784948 26.626302365311965 30.403111640478997 21.429348436359554 32-33 21.459953067906156 26.812527791048414 30.301028124666317 21.42649101637911 34-35 21.823161354098367 26.788568002678844 30.176520834210574 21.21174980901222 36-37 21.751944696011297 26.578585821923422 30.163900155435307 21.505569326629974 38-39 21.534552947428583 26.91174258756054 30.23006888530146 21.323635579709418 40-41 21.81537506779904 26.78355338153039 30.14626045057663 21.25481110009394 42-43 21.63535670101439 26.862231138327285 29.83996126106075 21.66245089959757 44-45 21.802057454739405 26.950331145746237 29.647594957521513 21.60001644199285 46-47 21.991108232629646 27.152849872097505 29.172471112283 21.683570782989843 48-49 22.003035319742487 27.36670346981411 28.931860706144203 21.6984005042992 50-51 21.904986776773743 27.56797660700862 28.780538057851334 21.7464985583663 52-53 22.166964427146933 27.673778831134715 28.46221750350395 21.6970392382144 54-55 22.01275895638434 27.52246552846001 28.60829522657023 21.856480288585416 56-57 22.01270583186955 27.799584125379983 28.469414968278027 21.718295074472437 58-59 22.19019595065392 27.49111479830968 28.57307921740046 21.74561003363594 60-61 22.1799831015668 27.40751917616253 28.868474195801756 21.544023526468905 62-63 21.936553419570366 27.51820722955346 28.856604030856044 21.688635320020133 64-65 21.899035611711213 27.537387371694148 28.658576168177568 21.90500084841707 66-67 21.943104750761833 27.729328238236388 28.400227785522684 21.927339225479095 68-69 21.891999735322955 27.510070761006656 28.622466268715375 21.975463234955015 70-71 21.99612607112028 27.481635431611217 28.530674117763827 21.991564379504673 72-73 22.04555434579917 27.387121708844386 28.56991756947243 21.997406375884008 74-75 21.90993296880232 27.820704078215762 28.190298021146003 22.07906493183591 76-77 21.992418637199407 27.641596637812977 28.32872656765379 22.03725815733383 78-79 21.9566713077435 27.66755185494776 28.25345542999723 22.122321407311514 80-81 22.046028177345335 27.832509969346297 28.099019231877847 22.022442621430525 82-83 22.049209914173417 27.742841100505334 28.21045058955643 21.99749839576482 84-85 21.964670422288087 27.73698952895985 28.11296942656799 22.185370622184067 86-87 21.93328853451986 27.742839103499044 28.172546782468512 22.151325579512587 88-89 22.035500704299483 27.600043110146423 28.06560762748823 22.29884855806586 90-91 21.871324186424108 27.559707180601954 28.26974935603944 22.299219276934494 92-93 22.027235092470036 27.498665966210154 28.152712217894777 22.321386723425032 94-95 21.847348127116657 27.434843590530217 28.17052251450204 22.54728576785109 96-97 21.83989577229113 27.499983708154396 28.15506628776011 22.505054231794364 98-99 21.686541037384004 27.49139790983245 27.989777749260096 22.832283303523447 100-101 21.53145053615832 27.713907533085212 28.012445820852466 22.742196109903997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 612.0 1 451.0 2 346.5 3 413.0 4 351.5 5 324.0 6 581.0 7 1276.5 8 2240.5 9 2804.0 10 2570.0 11 2239.0 12 2277.0 13 2447.0 14 2634.5 15 2876.0 16 3123.0 17 3315.0 18 3571.0 19 3912.5 20 4261.5 21 4826.0 22 5788.0 23 6959.5 24 8392.5 25 10163.5 26 12866.0 27 16392.0 28 19113.5 29 22077.5 30 26064.5 31 29784.5 32 33581.5 33 37861.0 34 43248.5 35 47704.5 36 52756.5 37 58442.5 38 60984.0 39 62342.5 40 63276.0 41 65103.5 42 67030.5 43 69182.0 44 71968.0 45 73815.0 46 73952.5 47 74827.5 48 79030.0 49 78580.5 50 73623.0 51 69614.5 52 65596.5 53 62610.0 54 59717.5 55 55002.0 56 49846.5 57 44393.5 58 39377.0 59 34635.5 60 29212.0 61 23587.5 62 18124.5 63 13799.0 64 10191.0 65 7548.0 66 5780.5 67 4433.0 68 3171.5 69 2062.5 70 1383.0 71 885.0 72 627.0 73 442.0 74 297.5 75 209.0 76 128.0 77 70.5 78 32.5 79 20.5 80 12.5 81 5.0 82 4.0 83 3.0 84 2.0 85 2.5 86 2.5 87 1.5 88 2.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029274767756851 2 0.004260882293377285 3 0.0 4 7.519204047136387E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010050669409672303 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.7596020235681934E-4 20-21 0.001127880607070458 22-23 0.0022306972006504613 24-25 0.011654766273061399 26-27 0.005839915143275926 28-29 3.2583217537591005E-4 30-31 0.0030076816188545547 32-33 0.004762162563186378 34-35 0.0 36-37 0.008596956627225935 38-39 0.013634823338807315 40-41 0.0013033287015036402 42-43 0.0 44-45 0.0 46-47 6.516643507518201E-4 48-49 0.0022306972006504613 50-51 0.005338634873466834 52-53 0.0 54-55 0.0029074255648927363 56-57 0.0030076816188545547 58-59 1.0025605396181848E-4 60-61 0.0016291608768795502 62-63 0.004010242158472739 64-65 2.506401349045462E-5 66-67 0.001654224890370005 68-69 4.010242158472739E-4 70-71 9.023044856563664E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0012281366610322763 80-81 0.0013534567284845494 82-83 0.00862202064071639 84-85 0.005238378819505016 86-87 0.002381081281593189 88-89 1.0025605396181848E-4 90-91 5.26344283299547E-4 92-93 1.253200674522731E-4 94-95 0.0 96-97 0.0014537127824463681 98-99 0.0014035847554654587 100-101 2.757041483950008E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1994892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.610285119525805 #Duplication Level Percentage of deduplicated Percentage of total 1 79.3978372984281 48.12325556531448 2 12.287239119100224 14.894661326809116 3 3.7076923151586367 6.741728650717192 4 1.5256539900847372 3.698812933311125 5 0.8224312576902213 2.4923896509907255 6 0.48473397728740575 1.7627918742306847 7 0.32041967068792365 1.3594509318809733 8 0.23095355116762728 1.1198528468509505 9 0.17114585678930655 0.9335879259322862 >10 0.9473481602586412 10.592043735726168 >50 0.07052003297056757 2.9750471380502317 >100 0.0322871963447841 3.3867383179877044 >500 0.0012411243083843202 0.48384654522206344 >1k 2.4822486167686003E-4 0.2737183295835395 >5k 2.4822486167686003E-4 1.1620742273927769 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9978 0.5001774532155124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7905 0.3962620532840876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5289 0.265127134702029 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2038 0.10216091898709304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.0128026980909245E-5 0.0 8 0.0 0.0 0.0 5.0128026980909245E-5 0.0 9 0.0 0.0 0.0 5.0128026980909245E-5 0.0 10-11 0.0 0.0 0.0 5.0128026980909245E-5 0.0 12-13 0.0 0.0 0.0 1.253200674522731E-4 0.0 14-15 0.0 0.0 0.0 2.0051210792363698E-4 0.0 16-17 0.0 0.0 0.0 2.0051210792363698E-4 0.0 18-19 0.0 0.0 0.0 2.0051210792363698E-4 0.0 20-21 0.0 0.0 0.0 2.2557612141409157E-4 0.0 22-23 0.0 0.0 0.0 2.757041483950008E-4 0.0 24-25 0.0 0.0 0.0 3.5089618886636467E-4 0.0 26-27 0.0 0.0 0.0 4.0102421584727396E-4 0.0 28-29 0.0 0.0 0.0 0.0011780086340513673 0.0 30-31 0.0 0.0 0.0 0.0028071695109309173 0.0 32-33 0.0 0.0 0.0 0.0052634428329954705 0.0 34-35 0.0 0.0 0.0 0.006466515480537292 0.0 36-37 0.0 0.0 0.0 0.008847596762130481 0.0 38-39 0.0 0.0 0.0 0.012381622664284584 0.0 40-41 0.0 0.0 0.0 0.016141224687852777 0.0 42-43 0.0 0.0 0.0 0.01960005854953551 0.0 44-45 0.0 0.0 0.0 0.021279347453395974 0.0 46-47 0.0 0.0 0.0 0.024111580977817345 0.0 48-49 0.0 0.0 0.0 0.027795990960914174 0.0 50-51 0.0 0.0 0.0 0.03208193726778191 0.0 52-53 0.0 0.0 0.0 0.04208247865047331 0.0 54-55 0.0 0.0 0.0 0.04935104256270515 0.0 56-57 0.0 0.0 0.0 0.06827437274799839 0.0 58-59 0.0 0.0 0.0 0.09561921146608438 0.0 60-61 0.0 0.0 0.0 0.11644740667665217 0.0 62-63 0.0 0.0 0.0 0.12805204492273264 0.0 64-65 0.0 0.0 0.0 0.15091042522602727 0.0 66-67 0.0 0.0 0.0 0.20372030165041516 0.0 68-69 0.0 0.0 0.0 0.2825967521048759 0.0 70-71 0.0 0.0 0.0 0.35014426846165103 0.0 72-73 0.0 0.0 0.0 0.4306498797929913 0.0 74-75 0.0 0.0 0.0 0.5218327608712652 0.0 76-77 0.0 0.0 0.0 0.5794799918993108 0.0 78-79 0.0 0.0 0.0 0.6538198559119992 0.0 80-81 0.0 0.0 0.0 0.762748058541515 0.0 82-83 0.0 0.0 0.0 0.8541815797546934 0.0 84-85 0.0 0.0 0.0 0.9877978356723071 0.0 86-87 0.0 0.0 0.0 1.1838736132081338 0.0 88-89 0.0 0.0 0.0 1.4409301355662363 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6460 0.0 38.17369 1 GTATCAA 10300 0.0 34.64432 1 ATCAACG 12800 0.0 27.56913 3 TATCAAC 12900 0.0 27.503374 2 TCAACGC 12945 0.0 27.26032 4 CAACGCA 13210 0.0 26.682861 5 AACGCAG 13550 0.0 26.013329 6 GTACATG 7965 0.0 23.444237 1 ACGCAGA 15210 0.0 22.955643 7 CGCAGAG 15210 0.0 22.76825 8 TACATGG 8250 0.0 22.279842 2 GAGTACT 8870 0.0 22.00708 12-13 ACATGGG 8025 0.0 21.720272 3 GCAGAGT 16205 0.0 21.0478 9 GTACTTT 9305 0.0 20.800404 14-15 GTATAGG 775 0.0 20.232128 1 AGAGTAC 13365 0.0 20.171036 10-11 AGTACTT 9350 0.0 20.140759 12-13 CATGGGG 3750 0.0 19.124493 4 CAGAGTA 15705 0.0 18.012548 10-11 >>END_MODULE