FastQCFastQC Report
Thu 26 May 2016
SRR936816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007535
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85390.4253475032813874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62570.31167576156829147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43300.21568739772905576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62650.035.0526961
GTATCAA97550.032.8422661
ATCAACG116450.026.944493
TATCAAC118300.026.7240582
TCAACGC118100.026.6082974
CAACGCA119350.026.3283045
AACGCAG122500.025.6468146
GTACATG80100.022.728291
GAGTACT71200.022.39093812-13
ACGCAGA138650.022.3779647
CGCAGAG138950.022.089738
TACATGG81900.021.6516232
ACATGGG81150.020.8558063
GTACTTT77350.020.45835514-15
GCAGAGT146950.020.3364839
ATACCGT1908.211322E-620.0126426
AGAGTAC116850.019.6600310-11
AGTACTT78700.019.17029212-13
GTGTAGC11650.018.7686211
CATGGGG39150.017.8505294
Produced by FastQC (version 0.11.5)