##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936816_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2007535 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60595506429527 31.0 31.0 34.0 30.0 34.0 2 31.693708951525128 33.0 31.0 34.0 30.0 34.0 3 31.709549273113545 33.0 31.0 34.0 30.0 34.0 4 35.31550483553213 37.0 35.0 37.0 33.0 37.0 5 35.31634915456019 37.0 35.0 37.0 33.0 37.0 6 35.37985240606017 37.0 35.0 37.0 33.0 37.0 7 35.31572201729982 37.0 35.0 37.0 33.0 37.0 8 35.27655458061752 37.0 35.0 37.0 32.0 37.0 9 36.87670650823024 39.0 37.0 39.0 33.0 39.0 10-11 36.74049244471454 39.0 37.0 39.0 32.0 39.0 12-13 36.646444769331545 39.0 37.0 39.0 32.0 39.0 14-15 37.75341575613875 40.0 37.5 41.0 32.0 41.0 16-17 37.67840461062946 40.0 37.0 41.0 32.0 41.0 18-19 37.633089584988554 40.0 37.0 41.0 32.0 41.0 20-21 37.566487010189114 40.0 37.0 41.0 32.0 41.0 22-23 37.44740813983318 40.0 37.0 41.0 32.0 41.0 24-25 37.41024888731704 40.0 37.0 41.0 32.0 41.0 26-27 37.43492442223921 40.0 37.0 41.0 32.0 41.0 28-29 37.35573551644181 40.0 37.0 41.0 31.5 41.0 30-31 37.19571165633476 39.5 36.5 41.0 31.0 41.0 32-33 37.00304129193265 39.0 36.0 41.0 31.0 41.0 34-35 36.8361027329536 39.0 36.0 41.0 30.0 41.0 36-37 36.65869138022501 39.0 35.5 41.0 30.0 41.0 38-39 36.499333760058974 39.0 35.0 41.0 30.0 41.0 40-41 36.315821891025564 39.0 35.0 41.0 30.0 41.0 42-43 36.094545798703386 39.0 35.0 40.0 29.5 41.0 44-45 35.844532473904565 39.0 35.0 40.0 28.5 41.0 46-47 35.51369316101587 38.5 35.0 40.0 27.0 41.0 48-49 35.266497470778845 38.5 35.0 40.0 26.0 41.0 50-51 34.57243111577133 38.0 34.0 39.5 25.0 40.5 52-53 34.769472512309875 38.0 34.5 40.0 26.0 40.5 54-55 35.15952997083488 39.0 35.0 40.0 26.0 41.0 56-57 35.04315167606045 38.5 34.5 40.0 26.0 41.0 58-59 34.82909911906891 38.0 34.0 40.0 26.0 41.0 60-61 34.53804641014976 38.0 34.0 40.0 24.5 41.0 62-63 34.228090917468435 38.0 34.0 40.0 23.5 41.0 64-65 33.89897984343984 37.0 33.5 40.0 23.0 41.0 66-67 33.521114949428025 37.0 33.0 39.5 22.0 41.0 68-69 33.1250132625334 36.0 33.0 39.0 22.0 41.0 70-71 32.714195020261165 36.0 32.0 39.0 20.5 40.5 72-73 32.322845927966384 35.0 32.0 38.5 20.0 40.0 74-75 31.9182218491832 35.0 31.5 37.5 18.5 39.5 76-77 31.454729058272957 35.0 31.0 37.0 17.5 39.0 78-79 30.98598031914761 35.0 31.0 36.5 12.5 39.0 80-81 30.538852373682154 34.5 30.5 36.0 7.5 37.5 82-83 30.12930334963027 34.0 30.0 35.5 7.0 37.0 84-85 29.748362295053386 34.0 30.0 35.0 3.5 37.0 86-87 29.38304587466719 34.0 29.0 35.0 2.0 36.0 88-89 29.009791859170576 34.0 29.0 35.0 2.0 36.0 90-91 28.633071901610684 34.0 29.0 35.0 2.0 36.0 92-93 28.231709783391075 34.0 29.0 35.0 2.0 35.0 94-95 27.83884988306555 34.0 27.5 35.0 2.0 35.0 96-97 27.318083370900133 34.0 27.0 35.0 2.0 35.0 98-99 26.713913331523486 33.5 25.5 35.0 2.0 35.0 100-101 24.435808840194568 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3599593154739509 1101 2 0.3416805629268467 1101 3 0.3361496837885376 1101 4 0.49457266080278117 1101 5 0.3855092273165823 1101 6 0.4866513208382557 1101 7 0.41335475623450435 1101 8 0.46270808165363064 1101 9 0.4865545353225258 1101 10-11 0.6403794457359453 1101 12-13 0.6862879254016434 1101 14-15 0.7404992580162357 1101 16-17 0.6139047930367383 1101 18-19 0.7412168652091964 1101 20-21 0.8437020475957411 1101 22-23 0.7761216145567857 1101 24-25 0.7005890389462479 1101 26-27 0.7580539574397065 1101 28-29 0.6322234697949938 1101 30-31 0.6052298246384566 1101 32-33 0.6131906551236952 1101 34-35 0.6964241548223526 1101 36-37 0.6404078964513715 1101 38-39 0.6511394947642231 1101 40-41 0.7102017106806571 1101 42-43 0.6855746314341644 1101 44-45 0.6867471861787564 1101 46-47 0.7075950821837722 1101 48-49 0.799387053265967 1101 50-51 0.7908400286375681 1101 52-53 0.6431855169593703 1101 54-55 0.933707459518935 1101 56-57 0.9259409124956051 1101 58-59 0.953618111340127 1101 60-61 1.0453118923570273 1101 62-63 1.0455019905410623 1101 64-65 0.9492565933280659 1101 66-67 0.8265570985300954 1101 68-69 0.8594716986581332 1101 70-71 0.8200588458759412 1101 72-73 0.9363396408766747 1101 74-75 0.9675457850316924 1101 76-77 1.0625568261794633 1101 78-79 1.1703638971722619 1101 80-81 1.1646274742652984 1101 82-83 1.0899717657728552 1101 84-85 1.0411245431966876 1101 86-87 1.0467333415826943 1101 88-89 1.01553962190917 1101 90-91 0.9252512542779208 1101 92-93 1.015477840824449 1101 94-95 1.0867720503435123 1101 96-97 1.1321085286424584 1101 98-99 0.9676721690670682 1101 100-101 1.3774632464324377 1102 1 0.20679623323960072 1102 2 0.1638089281287911 1102 3 0.23883781930499026 1102 4 0.17732712530192885 1102 5 0.13637419650481064 1102 6 0.006021598106485726 1102 7 0.12256280931782015 1102 8 -0.09548704533013108 1102 9 0.11516759025530376 1102 10-11 0.04042612261868328 1102 12-13 -0.06878551205510774 1102 14-15 -0.027746244773048545 1102 16-17 0.16054518980282495 1102 18-19 -0.012329193928273696 1102 20-21 0.007096299922665139 1102 22-23 0.008585701036096793 1102 24-25 0.08944081279360461 1102 26-27 0.07219755616446122 1102 28-29 -0.15883205682341384 1102 30-31 -0.03210526505413469 1102 32-33 -0.1791035073722469 1102 34-35 -0.11960098778747863 1102 36-37 -0.09886648561464284 1102 38-39 -0.0590410982209022 1102 40-41 -0.27640479187461864 1102 42-43 -0.09550198156038192 1102 44-45 0.008801950366141398 1102 46-47 0.1169966367378521 1102 48-49 0.07777817283174926 1102 50-51 -0.0968800509773402 1102 52-53 0.12482174929198919 1102 54-55 -0.2092508734998617 1102 56-57 -0.054305078450838096 1102 58-59 -0.22867964942764019 1102 60-61 -0.15448592502690417 1102 62-63 -0.3031101585330802 1102 64-65 -0.03310684219115956 1102 66-67 -0.3936502172849359 1102 68-69 -0.19737218918650257 1102 70-71 -0.28239502453090637 1102 72-73 -0.41010348000764907 1102 74-75 -0.1470170249418139 1102 76-77 -0.3152900233196725 1102 78-79 -0.2993269534038454 1102 80-81 -0.29193094229379213 1102 82-83 -0.13453649138028467 1102 84-85 -0.3653185021994112 1102 86-87 -0.26203989935305927 1102 88-89 -0.1628151374972937 1102 90-91 -0.3986504550270098 1102 92-93 -0.14004613549237988 1102 94-95 -0.04201525656391425 1102 96-97 -0.20326298663258413 1102 98-99 -0.26207622869545943 1102 100-101 -0.008335028913361242 1103 1 0.1579116206216611 1103 2 0.24066774481785203 1103 3 0.18134423073074046 1103 4 0.18926094166311458 1103 5 0.09536690419108851 1103 6 0.13936733103587784 1103 7 0.18813998070766047 1103 8 0.25815142139622793 1103 9 0.10020878910547992 1103 10-11 0.1675549683315367 1103 12-13 0.16884271149211827 1103 14-15 0.3479191902798888 1103 16-17 0.3467626811022413 1103 18-19 0.32135496451471823 1103 20-21 0.2646862834335906 1103 22-23 0.3936086581728375 1103 24-25 0.4484605849828185 1103 26-27 0.38242452043733266 1103 28-29 0.48865597180375175 1103 30-31 0.3494692531400858 1103 32-33 0.27852594023767097 1103 34-35 0.4375747617820167 1103 36-37 0.3275015733438451 1103 38-39 0.44169940009183506 1103 40-41 0.41139126338465815 1103 42-43 0.26504280184609996 1103 44-45 0.3513092957358239 1103 46-47 0.2856885707608754 1103 48-49 0.46952999730116574 1103 50-51 0.560739878352905 1103 52-53 0.5787535737693403 1103 54-55 0.6862543126709255 1103 56-57 0.6636847880587666 1103 58-59 0.6399411657583656 1103 60-61 0.6477431010762302 1103 62-63 0.7382249083591645 1103 64-65 0.8174457346793815 1103 66-67 0.726354424615117 1103 68-69 0.8813559063507981 1103 70-71 0.7649913035631997 1103 72-73 0.7296452334349013 1103 74-75 0.5120104771237983 1103 76-77 0.5141091208697439 1103 78-79 0.6106772155629088 1103 80-81 0.6485831878805008 1103 82-83 0.6021048316248248 1103 84-85 0.3726436559820776 1103 86-87 0.41284090356847614 1103 88-89 0.5545892256503144 1103 90-91 0.5595701592219555 1103 92-93 0.39056377758724636 1103 94-95 0.44054403595964686 1103 96-97 0.5643053929667445 1103 98-99 0.40072033327808043 1103 100-101 0.4678560576052213 1104 1 0.1266272312506267 1104 2 0.022836072959282916 1104 3 0.06402648823203805 1104 4 0.22774902264496433 1104 5 0.17770312249960085 1104 6 0.07690035173707344 1104 7 0.08410351441096964 1104 8 0.1961001231573931 1104 9 0.06787700972618183 1104 10-11 0.16185518593771064 1104 12-13 0.040670011498633585 1104 14-15 -0.19277474259165928 1104 16-17 0.1195393201028665 1104 18-19 0.1418695251889588 1104 20-21 0.09058822452801252 1104 22-23 0.02747689891668159 1104 24-25 0.10986971812899782 1104 26-27 -0.07183349774847159 1104 28-29 -0.03003487946449468 1104 30-31 0.23304334084149048 1104 32-33 0.07439704557050675 1104 34-35 0.0766509515630105 1104 36-37 0.026691419441242203 1104 38-39 0.1243246420134767 1104 40-41 0.12060263335658306 1104 42-43 0.084400676210592 1104 44-45 0.03794466630750293 1104 46-47 0.13566341682867034 1104 48-49 0.32228094969809007 1104 50-51 0.199664158111986 1104 52-53 0.2900766903316807 1104 54-55 0.4359739696304814 1104 56-57 0.3030701245986478 1104 58-59 0.27100114259702224 1104 60-61 0.18304896302750961 1104 62-63 0.08933700809105716 1104 64-65 0.10722071056120086 1104 66-67 -0.1607146786619893 1104 68-69 -0.020130543077968355 1104 70-71 -0.030518867503268154 1104 72-73 0.19900103629910149 1104 74-75 0.1665233884892281 1104 76-77 0.15771361030411057 1104 78-79 0.1690779384178711 1104 80-81 0.13051427991627307 1104 82-83 -0.0500630427594011 1104 84-85 0.29236719947075684 1104 86-87 0.276020183041485 1104 88-89 0.22042239293481458 1104 90-91 0.1260061330934299 1104 92-93 0.1219719637618546 1104 94-95 0.43119456596779315 1104 96-97 0.2677366852475451 1104 98-99 0.06297572361778592 1104 100-101 0.22114066793628595 1105 1 0.06883383615169691 1105 2 0.10862726165760961 1105 3 0.13888782208292483 1105 4 0.07344398776136529 1105 5 0.10317070577590215 1105 6 0.06253831817117828 1105 7 0.02280541538852532 1105 8 0.021723993143197617 1105 9 -0.0026654992373167374 1105 10-11 0.08256879309625731 1105 12-13 0.20756327686937937 1105 14-15 0.35588864852348934 1105 16-17 0.3285952666589438 1105 18-19 0.15005976595221426 1105 20-21 0.20480265397937814 1105 22-23 0.20387556281853847 1105 24-25 0.13993703377708044 1105 26-27 0.20027545349620368 1105 28-29 0.13242260135718453 1105 30-31 0.09711323062360577 1105 32-33 0.11705769911453245 1105 34-35 -0.09641461735125745 1105 36-37 -0.06328529077460132 1105 38-39 0.21149687405327455 1105 40-41 0.15626307238944293 1105 42-43 0.10390076303654183 1105 44-45 0.054565706788821444 1105 46-47 0.040290729208010134 1105 48-49 -0.005524294589271506 1105 50-51 -0.15953888181773834 1105 52-53 -0.06469567245778052 1105 54-55 -0.11841023008962992 1105 56-57 0.02832626115033321 1105 58-59 0.005418438326209696 1105 60-61 0.06227508844504115 1105 62-63 0.0032398856051756297 1105 64-65 0.12248318029980254 1105 66-67 -0.04793492034458069 1105 68-69 -0.012213548685899411 1105 70-71 -0.1705497162524665 1105 72-73 -0.11677370654286534 1105 74-75 0.16643094438981976 1105 76-77 0.16900200524981912 1105 78-79 0.23913217909940343 1105 80-81 0.03612987589980676 1105 82-83 0.11628526209161905 1105 84-85 0.06951512024463469 1105 86-87 -0.04453151925786969 1105 88-89 0.10387694210383813 1105 90-91 0.13514226596777945 1105 92-93 0.3319012772239667 1105 94-95 0.40219316653778137 1105 96-97 0.18643644111340407 1105 98-99 0.11645591197902405 1105 100-101 0.1754666431959926 1106 1 0.20031594501858407 1106 2 0.26755187684334203 1106 3 0.24155845316494862 1106 4 0.2900062690535705 1106 5 0.22034570321270053 1106 6 0.2242782180503724 1106 7 0.30204907091233224 1106 8 0.368471627198538 1106 9 0.40051063938051357 1106 10-11 0.49065368180881563 1106 12-13 0.45480215369760657 1106 14-15 0.3876250713667204 1106 16-17 0.4993516111351326 1106 18-19 0.5739680658153574 1106 20-21 0.3868116675264446 1106 22-23 0.2321775211030257 1106 24-25 0.21692986573371087 1106 26-27 0.3041928025683305 1106 28-29 0.22353676849563442 1106 30-31 0.43270526286197253 1106 32-33 0.4008237302562918 1106 34-35 0.4884558184818246 1106 36-37 0.3797905041516003 1106 38-39 0.3698069899990486 1106 40-41 0.49920324062449595 1106 42-43 0.38822791200079365 1106 44-45 0.3802548731471589 1106 46-47 0.42941785944975663 1106 48-49 0.38358166139502003 1106 50-51 0.37633699691394895 1106 52-53 0.35781624084811625 1106 54-55 0.3984503853457326 1106 56-57 0.41239809314708253 1106 58-59 0.48221234370625154 1106 60-61 0.39897921497348676 1106 62-63 0.36889568579150733 1106 64-65 0.1361025741365367 1106 66-67 0.2007766818111847 1106 68-69 0.2908461315159485 1106 70-71 0.5185390774389447 1106 72-73 0.3828722375838467 1106 74-75 0.6095518739174288 1106 76-77 0.6695840744428345 1106 78-79 0.6153533765370973 1106 80-81 0.5606258171289866 1106 82-83 0.7152713494900347 1106 84-85 0.7130924107420746 1106 86-87 0.7897098182419633 1106 88-89 0.6471974668688034 1106 90-91 0.7937935103454556 1106 92-93 1.0467181310413487 1106 94-95 0.8702020953878176 1106 96-97 1.0266484734409254 1106 98-99 1.0772035192879308 1106 100-101 1.3297815110909177 1107 1 0.3610334591990032 1107 2 0.4312629241577497 1107 3 0.3856126119260104 1107 4 0.3084323669803979 1107 5 0.40757041438915564 1107 6 0.40276818993788766 1107 7 0.3876158405587944 1107 8 0.4710608570355248 1107 9 0.48660638078534646 1107 10-11 0.555782025754695 1107 12-13 0.5225552406079146 1107 14-15 0.4641099174433876 1107 16-17 0.525967582605432 1107 18-19 0.5503419023258331 1107 20-21 0.5938467435692374 1107 22-23 0.5871682330543067 1107 24-25 0.691985991375681 1107 26-27 0.760908757151256 1107 28-29 0.6916400198093697 1107 30-31 0.7411606156588135 1107 32-33 0.7255056021706281 1107 34-35 0.5466869170543518 1107 36-37 0.6745926736808414 1107 38-39 0.6950526017685519 1107 40-41 0.8104708357649102 1107 42-43 0.9580946508526509 1107 44-45 0.9199334669266577 1107 46-47 0.8613821575812182 1107 48-49 0.7915202112673683 1107 50-51 0.8428523395783714 1107 52-53 0.9725844866486355 1107 54-55 0.9452623992132345 1107 56-57 0.8939501409879895 1107 58-59 1.0050033278420685 1107 60-61 1.0055558488256864 1107 62-63 1.137043638943986 1107 64-65 1.067558513024153 1107 66-67 1.1846211889485758 1107 68-69 0.9408662473433793 1107 70-71 1.0533012139881563 1107 72-73 1.0764463482655984 1107 74-75 1.0994420564002674 1107 76-77 1.1542297378677908 1107 78-79 1.0737545904403625 1107 80-81 1.1197792636407549 1107 82-83 1.1470722375665652 1107 84-85 1.083350787369966 1107 86-87 1.0751665716964247 1107 88-89 1.1037075446500033 1107 90-91 1.270697808675596 1107 92-93 1.2837074125337935 1107 94-95 1.3278791395035654 1107 96-97 1.3717896319139093 1107 98-99 1.9072997772473421 1107 100-101 1.994256463160614 1108 1 -0.12328574265411163 1108 2 -0.04123628751097286 1108 3 0.011681600988953988 1108 4 0.05471559247634161 1108 5 0.17663179042435928 1108 6 0.06678898765800767 1108 7 -0.09982776115490566 1108 8 -0.07839442383564688 1108 9 -0.14427794908565517 1108 10-11 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-2.2730425795625564 2316 68-69 -2.462809498346097 2316 70-71 -2.173023407398759 2316 72-73 -2.5773701974406578 2316 74-75 -2.786423142660013 2316 76-77 -2.8707804878317553 2316 78-79 -3.2395687094715413 2316 80-81 -2.9221508240380665 2316 82-83 -3.5942623997619556 2316 84-85 -3.284309782332599 2316 86-87 -3.5387692213983435 2316 88-89 -3.594642184762254 2316 90-91 -4.013791378302631 2316 92-93 -4.079176444495193 2316 94-95 -4.03028673707486 2316 96-97 -4.306392396025849 2316 98-99 -4.854138060987335 2316 100-101 -4.885696970576856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4289.0 3 1574.0 4 1640.0 5 1760.0 6 2019.0 7 2411.0 8 2907.0 9 3668.0 10 4671.0 11 5908.0 12 7289.0 13 8938.0 14 10102.0 15 11988.0 16 14862.0 17 18885.0 18 17208.0 19 12003.0 20 11553.0 21 12632.0 22 13476.0 23 15243.0 24 17373.0 25 19841.0 26 23443.0 27 27282.0 28 32555.0 29 39119.0 30 47957.0 31 59089.0 32 74166.0 33 96390.0 34 129008.0 35 181101.0 36 268189.0 37 391659.0 38 353235.0 39 62069.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28757907745667 19.349191597296105 14.769065173029821 22.59416415221741 2 15.002648311682618 20.523892355472686 40.83562081358726 23.637838519257436 3 18.903658838576725 25.2973214290163 30.126960447991358 25.67205928441562 4 12.562875928654977 17.238457816001844 38.20090494490242 31.997761310440755 5 13.552883781136998 37.50276740071763 34.458826063629274 14.485522754516097 6 30.578413887758437 37.010349587851806 18.053028670832035 14.358207853557719 7 27.45923190450818 33.19549994592 21.479678630225884 17.86558951934594 8 25.694404973091338 35.08765631902293 20.255634764174037 18.96230394371169 9 25.993396714110023 17.240727807131808 21.051101935172543 35.71477354358563 10-11 24.207629706252572 27.1850596343424 27.822280935704587 20.785029723700447 12-13 25.445021427926946 24.66831204166245 28.182646594487508 21.704019935923096 14-15 22.52869117116704 25.849407459118805 26.794028458012953 24.827872911701203 16-17 21.582768594032146 29.159686859388263 27.246841704699452 22.01070284188014 18-19 21.47693408895888 27.76462824055371 29.74068093529223 21.01775673519518 20-21 22.278016083385584 26.87639498498226 29.80615004580585 21.039438885826307 22-23 21.96399914590967 26.79162762259672 29.73569791114122 21.50867532035239 24-25 21.81010546651891 26.953108361673365 29.829157739961175 21.40762843184655 26-27 21.65176647808758 27.12883735689069 29.714555990652908 21.50484017436882 28-29 21.43316875703966 27.220223206702077 29.848329436872095 21.498278599386165 30-31 21.650797193210384 27.000928555985848 29.78323398514908 21.565040265654687 32-33 21.489825467354493 27.13593948757827 29.69685255402601 21.677382491041225 34-35 21.900401568013248 27.192645541628618 29.51123877330711 21.395714117051025 36-37 21.865605859116872 26.96306643190214 29.51128610793606 21.66004160104493 38-39 21.595983365978363 27.27681658141764 29.631119448933273 21.496080603670723 40-41 21.85442043383491 27.10243942778049 29.55462017215007 21.488519966234534 42-43 21.672372885324183 27.157615672349973 29.370064345025227 21.799947097300617 44-45 21.804897427499366 27.294534323462344 29.150914142464508 21.749654106573782 46-47 21.948646830458227 27.440030226793105 28.7899494418529 21.821373500895767 48-49 21.957388368873744 27.59910576545172 28.57304036259537 21.87046550307916 50-51 21.87174965628549 27.731284994121985 28.46783031462331 21.929135034969217 52-53 22.064654552935988 27.849699473172024 28.25004489657349 21.8356010773185 54-55 21.92105225793611 27.77396800471487 28.275496328460946 22.02948340888807 56-57 21.997882277863976 28.066369242365102 28.142763664750287 21.79298481502063 58-59 22.139955046686886 27.73488404994281 28.238292411737277 21.886868491633024 60-61 22.071241840483953 27.689900531613894 28.5449560442188 21.69390158368336 62-63 21.911543202788366 27.759800711538123 28.590277239821926 21.738378845851592 64-65 21.86583191965826 27.792681799943164 28.452764904361178 21.888721376037402 66-67 21.860466159108725 28.003825493140095 28.15421948953732 21.98148885821386 68-69 21.71641774308455 27.879291686009672 28.333955050473712 22.070335520432067 70-71 21.940463477213058 27.775635857490165 28.2775181028263 22.006382562470485 72-73 21.94127119140102 27.696589823644207 28.289656392010254 22.072482592944525 74-75 21.777632525197017 28.04761184746254 28.04965446187946 22.125101165460983 76-77 21.958460373733573 27.867741632522808 28.110358798876874 22.06343919486675 78-79 21.87155890163877 27.913317953751637 28.07993562499066 22.13518751961893 80-81 21.83610681206191 28.091595214100956 27.987005557758742 22.085292416078392 82-83 21.921862667719022 28.005812864238276 28.084739462501894 21.987585005540808 84-85 21.882579409985162 28.0287562051992 27.90164443227767 22.187019952537966 86-87 21.844311389180668 27.969252341478096 28.06689282518093 22.119543444160307 88-89 21.946070458611167 27.869718077343148 27.909612910476344 22.27459855356934 90-91 21.906464602166828 27.846577827391332 28.097148438668007 22.14980913177383 92-93 21.9726625280384 27.82662712427795 28.037329383745725 22.16338096393792 94-95 21.8186474018878 27.78201379943909 28.044386975150896 22.354951823522217 96-97 21.825008637695326 27.760344621001344 28.051448031464876 22.363198709838453 98-99 21.659773162175092 27.85002391963004 27.9305284244937 22.559674493701166 100-101 21.605891160691563 27.88826875242563 28.01456986954368 22.49127021733912 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 402.0 1 297.5 2 263.0 3 346.0 4 308.0 5 325.5 6 591.5 7 1289.0 8 2220.0 9 2621.5 10 2300.0 11 2048.0 12 2167.5 13 2303.5 14 2453.5 15 2639.5 16 2754.0 17 2895.0 18 3146.5 19 3451.5 20 3864.5 21 4479.0 22 5397.5 23 6684.5 24 8204.0 25 10120.5 26 12996.5 27 16320.5 28 19254.0 29 22334.0 30 26252.5 31 30160.5 32 34216.5 33 38726.5 34 44190.5 35 48922.5 36 53816.5 37 59284.5 38 61826.5 39 63003.0 40 64293.0 41 66224.0 42 68000.5 43 70207.0 44 72750.0 45 75156.5 46 75309.5 47 75123.0 48 79065.5 49 78638.5 50 73528.5 51 69744.5 52 65996.0 53 63035.5 54 59965.5 55 55048.5 56 49948.5 57 44540.5 58 39385.5 59 34680.5 60 28977.5 61 23266.5 62 18276.5 63 14005.5 64 10405.5 65 7712.5 66 5707.0 67 4299.0 68 3139.0 69 2062.5 70 1394.5 71 958.0 72 650.0 73 435.5 74 303.0 75 220.5 76 144.0 77 80.0 78 41.0 79 27.0 80 22.5 81 18.0 82 14.5 83 10.5 84 7.5 85 6.5 86 8.5 87 5.5 88 1.5 89 4.0 90 4.5 91 2.5 92 2.0 93 2.0 94 1.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03984986563123433 2 0.02968814989526957 3 0.026500160644770825 4 0.050011581367199076 5 0.10176659435576466 6 0.1210937791869133 7 0.06216579038472555 8 0.09270074992465885 9 0.0779064873090631 10-11 0.07770723798090694 12-13 0.0751169967148767 14-15 0.06906479837213299 16-17 0.05738380650897743 18-19 0.06667380643425892 20-21 0.06181710406045225 22-23 0.035939597566169455 24-25 0.010734557554413746 26-27 0.0032876139145768322 28-29 0.007870348462168778 30-31 0.00582804284856802 32-33 0.007247694311680743 34-35 0.007671099134012608 36-37 0.006649946327212228 38-39 0.004134423559240561 40-41 0.0078952546281883 42-43 0.003785737234967261 44-45 0.0026151474320497527 46-47 0.002216648775737409 48-49 3.984986563123433E-4 50-51 0.00273967826214736 52-53 0.007148069647602656 54-55 0.013474235816561105 56-57 0.00943943692139863 58-59 0.005927667512646105 60-61 0.0031381769184597027 62-63 0.009638686249554803 64-65 0.003013646088362096 66-67 0.023586139220486815 68-69 0.04958817654486721 70-71 0.06413337750026774 72-73 0.0043585790534162545 74-75 0.015118042773849523 76-77 0.023088015900096386 78-79 0.027147720961278383 80-81 0.07972463742848816 82-83 0.030236085547699045 84-85 0.012278739847624075 86-87 0.03402182278266631 88-89 0.05028554919341381 90-91 0.045578283815724256 92-93 0.04542884681960713 94-95 0.013922546804912492 96-97 0.017160348387450283 98-99 0.040597050611819965 100-101 0.08042201007703477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2007535.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.54012656309342 #Duplication Level Percentage of deduplicated Percentage of total 1 80.48181251151705 50.333427404974316 2 11.85106856997411 14.823346565481588 3 3.380075914085026 6.341711263792236 4 1.4175385669713907 3.5461216554582737 5 0.767456508984182 2.3998413601770294 6 0.4629669222777515 1.7372405948265848 7 0.2973684127595199 1.3018220718892606 8 0.22384734016361063 1.1199552787715226 9 0.15747993406593472 0.8863933506868048 >10 0.8615450045871081 9.967193615227492 >50 0.0703725046019246 3.038443733881761 >100 0.0273519700324966 3.0632481455232146 >500 5.579199899816787E-4 0.214289101130015 >1k 3.9851427855833963E-4 0.4894404940673663 >5k 1.5940571142333587E-4 0.7375253641125116 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8539 0.4253475032813874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6257 0.31167576156829147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4330 0.21568739772905576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.9812332039042905E-5 0.0 10-11 0.0 0.0 0.0 4.9812332039042905E-5 0.0 12-13 0.0 0.0 0.0 7.471849805856436E-5 0.0 14-15 0.0 0.0 0.0 1.4943699611712872E-4 0.0 16-17 0.0 0.0 0.0 1.4943699611712872E-4 0.0 18-19 0.0 0.0 0.0 1.4943699611712872E-4 0.0 20-21 0.0 0.0 0.0 2.2415549417569307E-4 0.0 22-23 0.0 0.0 0.0 3.4868632427330034E-4 0.0 24-25 0.0 0.0 0.0 4.981233203904291E-4 0.0 26-27 0.0 0.0 0.0 6.973726485466007E-4 0.0 28-29 0.0 0.0 0.0 0.0016687131233079373 0.0 30-31 0.0 0.0 0.0 0.0028393029262254454 0.0 32-33 0.0 0.0 0.0 0.004483109883513862 0.0 34-35 0.0 0.0 0.0 0.00600238601070467 0.0 36-37 0.0 0.0 0.0 0.008343565616539686 0.0 38-39 0.0 0.0 0.0 0.012179115183545992 0.0 40-41 0.0 0.0 0.0 0.01628863257676703 0.0 42-43 0.0 0.0 0.0 0.019626058823382905 0.0 44-45 0.0 0.0 0.0 0.021419302776788447 0.0 46-47 0.0 0.0 0.0 0.023885013212721073 0.0 48-49 0.0 0.0 0.0 0.02759603194962977 0.0 50-51 0.0 0.0 0.0 0.032128954165182674 0.0 52-53 0.0 0.0 0.0 0.041319329426386095 0.0 54-55 0.0 0.0 0.0 0.04801908808563736 0.0 56-57 0.0 0.0 0.0 0.06567755979347807 0.0 58-59 0.0 0.0 0.0 0.09095731830329234 0.0 60-61 0.0 0.0 0.0 0.11210265325386606 0.0 62-63 0.0 0.0 0.0 0.1224885244840065 4.9812332039042905E-5 64-65 0.0 0.0 0.0 0.1433598916083655 4.9812332039042905E-5 66-67 0.0 0.0 0.0 0.19523943542702868 4.9812332039042905E-5 68-69 0.0 0.0 0.0 0.27309611040405274 4.9812332039042905E-5 70-71 0.0 0.0 0.0 0.33962047984219457 4.9812332039042905E-5 72-73 0.0 0.0 0.0 0.4169043130007696 4.9812332039042905E-5 74-75 0.0 0.0 0.0 0.5064668860069688 4.9812332039042905E-5 76-77 0.0 0.0 0.0 0.5617834807363259 4.9812332039042905E-5 78-79 0.0 0.0 0.0 0.6310973407686541 4.9812332039042905E-5 80-81 0.0 0.0 0.0 0.7385425409768696 4.9812332039042905E-5 82-83 0.0 0.0 0.0 0.8302719504267672 4.9812332039042905E-5 84-85 0.0 0.0 0.0 0.9548774990224329 4.9812332039042905E-5 86-87 0.0 0.0 0.0 1.1484731274921733 4.9812332039042905E-5 88-89 0.0 0.0 0.0 1.4057040101417908 4.9812332039042905E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6265 0.0 35.052696 1 GTATCAA 9755 0.0 32.842266 1 ATCAACG 11645 0.0 26.94449 3 TATCAAC 11830 0.0 26.724058 2 TCAACGC 11810 0.0 26.608297 4 CAACGCA 11935 0.0 26.328304 5 AACGCAG 12250 0.0 25.646814 6 GTACATG 8010 0.0 22.72829 1 GAGTACT 7120 0.0 22.390938 12-13 ACGCAGA 13865 0.0 22.377964 7 CGCAGAG 13895 0.0 22.08973 8 TACATGG 8190 0.0 21.651623 2 ACATGGG 8115 0.0 20.855806 3 GTACTTT 7735 0.0 20.458355 14-15 GCAGAGT 14695 0.0 20.336483 9 ATACCGT 190 8.211322E-6 20.012642 6 AGAGTAC 11685 0.0 19.66003 10-11 AGTACTT 7870 0.0 19.170292 12-13 GTGTAGC 1165 0.0 18.768621 1 CATGGGG 3915 0.0 17.850529 4 >>END_MODULE