FastQCFastQC Report
Thu 26 May 2016
SRR936816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007535
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99190.4940885214952666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80870.40283232919974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53940.26868771901859745No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20760.10341040131305308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70700.034.5437741
GTATCAA110250.033.8742751
ATCAACG136850.027.143023
TATCAAC137900.027.0748062
TCAACGC137850.026.9454464
CAACGCA139150.026.7278465
AACGCAG141750.026.2382556
ACGCAGA159700.023.2593777
CGCAGAG160000.022.9805538
GAGTACT92300.022.1821812-13
GCAGAGT164250.022.067789
GTACATG76300.021.6710911
AGTACTT95950.021.66016412-13
AGAGTAC134000.021.46588710-11
GTACTTT96450.020.97990814-15
TACATGG78700.020.4008852
ACATGGG77000.019.9871333
TACTTTT110650.018.5236114-15
GGATCGC1801.2338461E-418.4723346
CAGAGTA162450.018.05746310-11
Produced by FastQC (version 0.11.5)