##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936816_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2007535 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.153803545143674 33.0 31.0 34.0 31.0 34.0 2 32.29624838421248 33.0 31.0 34.0 31.0 34.0 3 32.41774365079563 34.0 31.0 34.0 31.0 34.0 4 36.079230997217984 37.0 35.0 37.0 35.0 37.0 5 35.923265098740494 37.0 35.0 37.0 35.0 37.0 6 36.00625742515074 37.0 35.0 37.0 35.0 37.0 7 35.967230957368116 37.0 35.0 37.0 35.0 37.0 8 35.91893341834638 37.0 35.0 37.0 35.0 37.0 9 37.62471638103445 39.0 37.0 39.0 35.0 39.0 10-11 37.538764454916105 39.0 37.0 39.0 35.0 39.0 12-13 37.43346716246541 39.0 37.0 39.0 34.5 39.0 14-15 38.64293299992279 40.0 38.0 41.0 34.0 41.0 16-17 38.6443476701527 40.0 38.0 41.0 34.5 41.0 18-19 38.77010014769357 40.0 38.0 41.0 35.0 41.0 20-21 38.77729080688506 40.0 38.0 41.0 34.5 41.0 22-23 38.690863422057404 40.0 38.0 41.0 34.0 41.0 24-25 38.60224180400341 40.0 38.0 41.0 34.0 41.0 26-27 38.41043468731554 40.0 38.0 41.0 34.0 41.0 28-29 38.26510621234499 40.0 38.0 41.0 34.0 41.0 30-31 38.23862971255794 40.0 38.0 41.0 34.0 41.0 32-33 38.104449735621046 40.0 37.0 41.0 33.5 41.0 34-35 37.98273056260538 40.0 37.0 41.0 33.0 41.0 36-37 37.83260117507291 40.0 37.0 41.0 33.0 41.0 38-39 37.682009778160776 40.0 37.0 41.0 33.0 41.0 40-41 37.36378593648429 40.0 36.5 41.0 32.5 41.0 42-43 37.17181518628567 40.0 36.0 41.0 32.0 41.0 44-45 36.94617926960178 39.5 36.0 41.0 31.0 41.0 46-47 36.58001529238594 39.0 36.0 41.0 30.5 41.0 48-49 36.22037174943401 39.0 36.0 40.5 30.0 41.0 50-51 35.95012116849769 39.0 35.0 40.0 29.0 41.0 52-53 35.69948668391834 39.0 35.0 40.0 28.5 41.0 54-55 35.31517457977071 38.0 35.0 40.0 27.0 41.0 56-57 35.13109584639869 38.0 34.0 40.0 27.0 41.0 58-59 34.81229667228716 38.0 34.0 40.0 26.5 41.0 60-61 34.44570978837231 38.0 34.0 40.0 26.0 41.0 62-63 34.07596131574294 37.0 33.0 39.5 26.0 41.0 64-65 33.7685631881885 37.0 33.0 39.0 25.0 40.0 66-67 33.43252371689659 36.0 33.0 39.0 24.5 40.0 68-69 32.984400022913675 36.0 32.0 39.0 23.0 40.0 70-71 32.547410879511446 35.0 32.0 38.0 22.0 40.0 72-73 32.130613663024555 35.0 31.0 37.5 21.5 39.5 74-75 31.63597845118516 35.0 31.0 37.0 20.0 39.0 76-77 30.20326669273512 33.5 29.5 35.5 19.5 38.0 78-79 30.881482016502826 34.0 30.5 36.0 19.5 38.0 80-81 30.740784594041948 34.0 31.0 36.0 18.0 37.0 82-83 30.384545723984886 34.0 30.0 35.0 17.5 37.0 84-85 29.93045202200709 34.0 30.0 35.0 12.0 36.5 86-87 29.552823487510803 34.0 29.5 35.0 7.5 36.0 88-89 29.166530346917988 34.0 29.0 35.0 4.5 36.0 90-91 28.703618367799315 33.5 29.0 35.0 2.0 35.0 92-93 28.21041003021118 33.0 29.0 35.0 2.0 35.0 94-95 27.65131043792512 33.0 27.0 34.5 2.0 35.0 96-97 26.871770604248493 32.5 25.5 34.0 2.0 35.0 98-99 26.004773764840962 32.0 24.5 34.0 2.0 35.0 100-101 22.753361460696823 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2004429935588874 1101 2 0.3111752432364767 1101 3 0.2924421486698918 1101 4 0.16254494613735204 1101 5 0.021960270103996038 1101 6 0.1090103700506333 1101 7 0.12336825999961576 1101 8 0.1757390706435089 1101 9 0.25571946724765837 1101 10-11 0.25246085679355446 1101 12-13 0.29637655215636727 1101 14-15 0.38257649799569293 1101 16-17 0.41036347438300425 1101 18-19 0.20159786150384207 1101 20-21 0.16748000831344 1101 22-23 0.2420994384663402 1101 24-25 0.1714805961742627 1101 26-27 0.2474245920626359 1101 28-29 0.3382708484195476 1101 30-31 0.5205132295981443 1101 32-33 0.497507941242624 1101 34-35 0.3644424001720026 1101 36-37 0.484200441444564 1101 38-39 0.6018880939038951 1101 40-41 0.6574866350193602 1101 42-43 0.6141437057373977 1101 44-45 0.4972188791357226 1101 46-47 0.40427382981109616 1101 48-49 0.37904252331029653 1101 50-51 0.533538751731875 1101 52-53 0.6925673555981504 1101 54-55 0.6327075809103277 1101 56-57 0.6823855541418453 1101 58-59 0.6369459005932612 1101 60-61 0.7861051795063929 1101 62-63 0.7108090402342526 1101 64-65 0.581347160022446 1101 66-67 0.6220795197886346 1101 68-69 0.3272452758418467 1101 70-71 0.15890728593989678 1101 72-73 0.3608564681513471 1101 74-75 0.42705892866800355 1101 76-77 0.44250742573553126 1101 78-79 0.36550055443149176 1101 80-81 0.30587718191719304 1101 82-83 0.6300961538681449 1101 84-85 0.35089005084138236 1101 86-87 0.05346168376259541 1101 88-89 0.1927335586135399 1101 90-91 0.3503151301240628 1101 92-93 0.3733821089296221 1101 94-95 0.12296157630551363 1101 96-97 0.025277494051618277 1101 98-99 0.322475066319722 1101 100-101 0.2926503245431107 1102 1 -0.1358268315349065 1102 2 -0.008891035420738547 1102 3 -0.01329143327131277 1102 4 -0.0027843208971916056 1102 5 -0.1420794065044717 1102 6 0.05105051468606803 1102 7 0.11096554544322146 1102 8 0.1787812459120559 1102 9 0.20392448318825274 1102 10-11 0.15240625364771176 1102 12-13 0.22709723819330918 1102 14-15 0.17082952444425104 1102 16-17 0.16196634092234774 1102 18-19 0.19895954821263473 1102 20-21 0.22972130389259604 1102 22-23 0.05359528787237622 1102 24-25 0.005527293187199689 1102 26-27 0.1173753737431511 1102 28-29 -0.05848580020543892 1102 30-31 0.054345162624997556 1102 32-33 -0.01743495191889366 1102 34-35 0.16277058772499942 1102 36-37 0.05571106137898596 1102 38-39 0.20409033691053935 1102 40-41 0.25561691762209904 1102 42-43 0.24986271845212826 1102 44-45 0.2676566856493494 1102 46-47 0.31074266701572384 1102 48-49 0.42523250703001025 1102 50-51 0.2927067960585603 1102 52-53 0.35571752934686884 1102 54-55 0.5653179887228603 1102 56-57 0.4504920327925177 1102 58-59 0.19611860002684978 1102 60-61 0.4123170799726523 1102 62-63 0.2949129824807244 1102 64-65 0.21701144377762915 1102 66-67 0.13582089211414683 1102 68-69 0.16775061914968603 1102 70-71 -0.0844837203935711 1102 72-73 0.09853049995201246 1102 74-75 0.2039855767645733 1102 76-77 0.2681831082119501 1102 78-79 -0.05000359364619911 1102 80-81 0.07635587788335485 1102 82-83 0.16654249558318668 1102 84-85 0.14001433716316924 1102 86-87 -0.024879361950553402 1102 88-89 0.20054910566409134 1102 90-91 0.12307231219913817 1102 92-93 -0.02602983783608437 1102 94-95 0.07812094451943352 1102 96-97 0.17954018802489102 1102 98-99 0.14554263079294927 1102 100-101 0.30035389740056573 1103 1 0.10776323305029933 1103 2 0.1371058622957122 1103 3 0.07814823534152993 1103 4 -0.016884887185156572 1103 5 -0.0090257794437818 1103 6 -0.012653006203080963 1103 7 -0.019099063605857225 1103 8 0.029922306816722255 1103 9 -0.016814755293736994 1103 10-11 0.05393478205808577 1103 12-13 0.060764704872376285 1103 14-15 0.1388008114309116 1103 16-17 0.14359635923162273 1103 18-19 0.12451100236334156 1103 20-21 0.04585268158707123 1103 22-23 0.11791382077760204 1103 24-25 0.10303908770681858 1103 26-27 0.05837893028113683 1103 28-29 0.07485695205182452 1103 30-31 0.10258585070720017 1103 32-33 0.11361774994116303 1103 34-35 0.11712119663657461 1103 36-37 -0.00724172755644048 1103 38-39 -0.053935222154265716 1103 40-41 0.09305264936319446 1103 42-43 -0.01245590268099761 1103 44-45 0.03193927164869592 1103 46-47 0.06521178745116885 1103 48-49 0.02449266446513576 1103 50-51 -0.06696375621229578 1103 52-53 0.04664070709308987 1103 54-55 0.21698044866759147 1103 56-57 -0.045074912904802034 1103 58-59 0.09227655339402929 1103 60-61 -0.016094229730704512 1103 62-63 0.2920565050231545 1103 64-65 0.14274491763971753 1103 66-67 0.0822157890444899 1103 68-69 0.13325847997694495 1103 70-71 0.14019666213397386 1103 72-73 0.23345161745766063 1103 74-75 0.0057160357761532055 1103 76-77 0.11990857956550727 1103 78-79 0.09575634063594052 1103 80-81 -0.030583881938060387 1103 82-83 0.2006627462298951 1103 84-85 0.19273577030212152 1103 86-87 0.01791613492947519 1103 88-89 0.11440191848463499 1103 90-91 -0.06838450521403061 1103 92-93 -0.026775303935711037 1103 94-95 0.14152381494643862 1103 96-97 0.29735532753425886 1103 98-99 0.2597079372795683 1103 100-101 0.6508159611941053 1104 1 -0.02151594403141388 1104 2 0.031746003236648335 1104 3 -0.038529117636201704 1104 4 -0.0387029147617568 1104 5 -0.22475103142699737 1104 6 -0.055567784680782495 1104 7 0.025648302136261236 1104 8 -0.09675802517572407 1104 9 -0.09168883766842129 1104 10-11 -0.021392564310573903 1104 12-13 -0.029163167460076522 1104 14-15 -0.0557747184292694 1104 16-17 -0.034750840001329664 1104 18-19 -0.030584000586081572 1104 20-21 -0.024530684068238884 1104 22-23 -0.0568277842208289 1104 24-25 -0.03874336925036914 1104 26-27 -0.02609261026404397 1104 28-29 -0.04311824518459417 1104 30-31 -0.06535600523869789 1104 32-33 0.06614507894867927 1104 34-35 0.1225283056310289 1104 36-37 -0.00863466354314113 1104 38-39 -0.006370813291397326 1104 40-41 0.11216371926350632 1104 42-43 0.08301234516513745 1104 44-45 0.12032012782936619 1104 46-47 0.15043281653306195 1104 48-49 0.17814649506376412 1104 50-51 0.19583061877660413 1104 52-53 0.18097020190349156 1104 54-55 0.07958917777256858 1104 56-57 0.23403479572387909 1104 58-59 -0.030852043371666582 1104 60-61 0.09089530820635616 1104 62-63 0.28838501735055644 1104 64-65 0.07932485091767916 1104 66-67 -0.14014635261062125 1104 68-69 0.07047988784745485 1104 70-71 0.06892264999300579 1104 72-73 0.03816576028069818 1104 74-75 -0.00558094042526136 1104 76-77 0.18725699999720646 1104 78-79 0.2677397923078395 1104 80-81 0.09719128728378479 1104 82-83 0.09690188811583056 1104 84-85 -0.11558820839891126 1104 86-87 -0.14139614669986855 1104 88-89 -0.08875317375631298 1104 90-91 0.06285036543556899 1104 92-93 0.09830997282659482 1104 94-95 0.21008366848150217 1104 96-97 0.33214489269674985 1104 98-99 0.129729480882677 1104 100-101 0.7926819686633415 1105 1 -0.0077616921135827965 1105 2 0.04723677414893501 1105 3 0.03110835848666227 1105 4 0.036989905242940324 1105 5 -0.059311845343664515 1105 6 0.038249186217200304 1105 7 -0.005438452309739716 1105 8 0.08753975718737195 1105 9 0.06528941498487484 1105 10-11 -0.003790901338270203 1105 12-13 -0.020318667628622222 1105 14-15 0.026516363013470823 1105 16-17 -0.1960943949621594 1105 18-19 -0.10661390957205441 1105 20-21 0.08369786226106868 1105 22-23 0.10778451237661812 1105 24-25 0.006459752397894647 1105 26-27 0.060206765610601565 1105 28-29 -0.02143065516651177 1105 30-31 -0.020477204049690556 1105 32-33 -0.10658033653903232 1105 34-35 -0.005265702356958002 1105 36-37 0.031617336007414565 1105 38-39 -0.11120752943183021 1105 40-41 -0.00645278583879616 1105 42-43 -0.08052024521046519 1105 44-45 -0.23250988739071232 1105 46-47 -0.3365856825016422 1105 48-49 -0.32470956794123396 1105 50-51 -0.28875266773881236 1105 52-53 -0.03320490050005276 1105 54-55 0.06911439304985123 1105 56-57 -0.12015736542507938 1105 58-59 -0.1008419307022308 1105 60-61 -0.22533552624209108 1105 62-63 -0.15242215753733745 1105 64-65 -0.10578807389104838 1105 66-67 -0.0595882149761664 1105 68-69 0.005730712693974738 1105 70-71 -0.15838257897213026 1105 72-73 -0.05327320107664235 1105 74-75 -0.03322019392639319 1105 76-77 -0.14190119688318603 1105 78-79 -0.01567236536808636 1105 80-81 -0.2090739711000289 1105 82-83 -0.3009070335058759 1105 84-85 -0.646008445995566 1105 86-87 -0.35385669196683267 1105 88-89 -0.14651132773447273 1105 90-91 -0.1716001284508586 1105 92-93 -0.23075016180470342 1105 94-95 -0.2584041299188584 1105 96-97 -0.13106235573782854 1105 98-99 0.0038997823219482086 1105 100-101 0.34642621005991003 1106 1 0.03634606925476902 1106 2 0.14205376169847028 1106 3 0.11377577999703448 1106 4 0.18394326392594706 1106 5 0.08360592275586498 1106 6 0.10147239855265155 1106 7 0.11848043468213376 1106 8 0.20834758854221036 1106 9 0.2907337400609933 1106 10-11 0.23368474826566654 1106 12-13 0.3250155290200567 1106 14-15 0.41363372189262293 1106 16-17 0.31080083481197107 1106 18-19 0.29261657333474744 1106 20-21 0.20883482627374406 1106 22-23 0.24233020196646038 1106 24-25 0.2764729918809792 1106 26-27 0.264198781801106 1106 28-29 0.3249890007852372 1106 30-31 0.1952977291956728 1106 32-33 0.1551986799947187 1106 34-35 0.13185232518295464 1106 36-37 0.1680662570118301 1106 38-39 0.36501322223202237 1106 40-41 0.46216986214697187 1106 42-43 0.4229906695620329 1106 44-45 0.3041136655159491 1106 46-47 0.22740314640589787 1106 48-49 0.25144052856879995 1106 50-51 0.41724076155477263 1106 52-53 0.3595308804288493 1106 54-55 0.3804412834652666 1106 56-57 0.35741285509924126 1106 58-59 0.45665464798354094 1106 60-61 0.28942672027975647 1106 62-63 0.4360807778834044 1106 64-65 0.392952837894768 1106 66-67 0.504282929637327 1106 68-69 0.34981807990909886 1106 70-71 0.45889461211773863 1106 72-73 0.3974013661884044 1106 74-75 0.2840573314938588 1106 76-77 0.392849437230101 1106 78-79 0.4007817043241957 1106 80-81 0.5641340180587555 1106 82-83 0.6638441801134043 1106 84-85 0.6417549506227935 1106 86-87 0.5964670512852663 1106 88-89 0.6743594691754247 1106 90-91 0.4921751747867944 1106 92-93 0.7746605296351028 1106 94-95 0.8688478080829185 1106 96-97 0.8598409603737771 1106 98-99 0.8022135313245258 1106 100-101 1.3182852875755238 1107 1 0.2425094594422177 1107 2 0.2432845523249867 1107 3 0.21349564847429292 1107 4 0.1113027759805405 1107 5 0.017034951136565724 1107 6 0.14931031961913988 1107 7 0.19024277983993443 1107 8 0.21540650556566732 1107 9 0.21081281264684293 1107 10-11 0.26958305654941483 1107 12-13 0.28396309873009073 1107 14-15 0.336118250089541 1107 16-17 0.3512351380096419 1107 18-19 0.3218413937823641 1107 20-21 0.3694291620540895 1107 22-23 0.23942656919702898 1107 24-25 0.3485651372712226 1107 26-27 0.4737776408115124 1107 28-29 0.4566222569665044 1107 30-31 0.36303169365274357 1107 32-33 0.38257880456384186 1107 34-35 0.41412307925330794 1107 36-37 0.3211233660942483 1107 38-39 0.303269304165525 1107 40-41 0.4761674979163857 1107 42-43 0.47234447964875415 1107 44-45 0.43398815268653124 1107 46-47 0.4017894894974816 1107 48-49 0.5079876095387377 1107 50-51 0.3873218208890066 1107 52-53 0.5658544522051017 1107 54-55 0.7552532873990216 1107 56-57 0.5602861899557467 1107 58-59 0.5096740088465879 1107 60-61 0.5739926654256351 1107 62-63 0.7775270810413417 1107 64-65 0.8335098228054107 1107 66-67 0.7489331129148624 1107 68-69 0.6633391444088872 1107 70-71 0.5032867664483618 1107 72-73 0.8064512534404358 1107 74-75 0.6264898068830149 1107 76-77 0.26201286715251726 1107 78-79 0.6452841231903612 1107 80-81 0.7671248194137164 1107 82-83 0.8653637942868713 1107 84-85 0.8159806154384732 1107 86-87 1.0528209209744404 1107 88-89 1.0840883276891375 1107 90-91 0.9284646241594814 1107 92-93 1.043456907792791 1107 94-95 1.2954573226209476 1107 96-97 1.1297536427821093 1107 98-99 1.3013247097643337 1107 100-101 1.5951016722894806 1108 1 0.026898840560676263 1108 2 0.06349618074474961 1108 3 0.021109624580148534 1108 4 0.04626366763655909 1108 5 -0.012522137087778162 1108 6 -0.0430821057620463 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2316 76-77 -0.9739614060362207 2316 78-79 -1.1346121515086374 2316 80-81 -1.3420229865182414 2316 82-83 -1.200515930510715 2316 84-85 -1.1707991282373236 2316 86-87 -1.2011314429975357 2316 88-89 -1.6240552621082358 2316 90-91 -1.685650774170849 2316 92-93 -1.381086082096875 2316 94-95 -1.515886386738451 2316 96-97 -1.4308278586652179 2316 98-99 -1.5522167908439677 2316 100-101 -2.1661319141517623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 9.0 5 18.0 6 77.0 7 185.0 8 483.0 9 1029.0 10 1751.0 11 2865.0 12 4412.0 13 6204.0 14 7943.0 15 10912.0 16 14501.0 17 19798.0 18 18209.0 19 11385.0 20 10030.0 21 10265.0 22 10730.0 23 11783.0 24 13891.0 25 16727.0 26 20213.0 27 25250.0 28 31589.0 29 39371.0 30 49268.0 31 62104.0 32 79668.0 33 104794.0 34 143229.0 35 203133.0 36 310046.0 37 453666.0 38 295094.0 39 16896.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20344015991104 19.99251662584524 15.391985316244803 22.41205789799892 2 15.321228834603676 21.048706750759543 40.13035358559535 23.499710829041433 3 19.148335240323043 25.171267933133652 30.534035158680307 25.146361667862998 4 12.957930994976428 17.717947632295328 37.80626489700055 31.517856475727697 5 13.459294940448022 37.705492928980966 34.38791724989913 14.447294880671885 6 30.732764310460343 37.16712286460759 17.740412994044934 14.359699830887132 7 27.41268641029243 33.3910658549245 21.269328439767396 17.926919295015676 8 25.903707780935324 35.15490389955841 20.010709651388396 18.930678668117864 9 25.943234806102815 17.026320364347686 20.948447671844 36.0819971577055 10-11 24.287583672306422 27.304867563886837 27.64643089481922 20.761117868987526 12-13 25.542301915647737 24.60769198197682 28.310513770196994 21.539492332178447 14-15 22.476494673271663 25.803268341925822 27.01942569019071 24.700811294611803 16-17 21.443910068815736 29.142605234777974 27.49518688341673 21.91829781298956 18-19 21.27356670757121 27.49769055792484 30.330066532354977 20.89867620214897 20-21 22.0797712992288 26.53087350822958 30.56536592023767 20.82398927230395 22-23 21.721872382480456 26.548382284191856 30.432055886244953 21.29768944708274 24-25 21.621493724488182 26.707406705419185 30.528080465229458 21.143019104863175 26-27 21.60391127405319 26.869137696572405 30.350206472630546 21.17674455674386 28-29 21.30088987307061 26.909214715470505 30.545293515649924 21.244601895808962 30-31 21.53414510830446 26.64780439693455 30.502905304266175 21.31514519049481 32-33 21.431129650172625 26.820704815313125 30.36393424167872 21.384231292835533 34-35 21.792023551270585 26.789744637079803 30.2757112578361 21.142520553813508 36-37 21.7526645187129 26.659533387928818 30.157853680114293 21.429948413243988 38-39 21.55549029468769 26.93096819977398 30.281765042443226 21.231776463095102 40-41 21.789144605428195 26.790071122649962 30.12144847518179 21.299335796740053 42-43 21.608963231027108 26.842246835048954 29.922317668185112 21.62647226573883 44-45 21.727678861633713 26.98033408077724 29.729139378488313 21.562847679100734 46-47 21.935280121801508 27.186712417781365 29.222125613658783 21.65588184675834 48-49 21.95533571685639 27.386032355640566 29.023091780581385 21.635540146921656 50-51 21.91510500479825 27.47113516863109 28.850535005293885 21.763224821276776 52-53 22.102854854328307 27.69251537213927 28.516987060703514 21.68764271282891 54-55 21.958620895775166 27.49966999330024 28.686224648636262 21.85548446228833 56-57 21.980739496535044 27.816797573931723 28.53362443761681 21.668838491916425 58-59 22.15962186405995 27.492836975948578 28.63909516759882 21.70844599239265 60-61 22.075162621379175 27.517178700578153 28.87752705416512 21.53013162387755 62-63 21.89139915368848 27.52335575718481 28.966518641019956 21.618726448106756 64-65 21.875968227686304 27.54610880811981 28.757968731787358 21.819954232406523 66-67 21.94353322446015 27.717879817258435 28.508501348295635 21.83008560998578 68-69 21.84674469778198 27.57975275618203 28.666933162974946 21.906569383061047 70-71 21.973472343467275 27.490308351940385 28.626357546783293 21.909861757809047 72-73 21.982718577516717 27.405099247508698 28.618330521837393 21.9938516531372 74-75 21.918887401674038 27.783251746840943 28.27538591897009 22.02247493251493 76-77 21.96831184551184 27.696712257460337 28.366190833772304 21.968785063255513 78-79 21.922118703610582 27.66477404192566 28.31303350710413 22.100073747359627 80-81 21.93748443337484 27.85143212951432 28.226948941469487 21.984134495641346 82-83 22.03383456212177 27.77976750568982 28.237717636522042 21.948680295666374 84-85 21.920710020264092 27.789699784606864 28.17575362691358 22.113836568215465 86-87 21.87673409612808 27.78708163763431 28.199130274516442 22.13705399172117 88-89 21.960231482807533 27.672855564840216 28.09928306083335 22.267629891518897 90-91 21.904664413625145 27.591116052559677 28.326305965827792 22.177913567987385 92-93 21.95334078857903 27.55017969798783 28.198263043981797 22.298216469451344 94-95 21.82108904701537 27.51309441678476 28.206631515764357 22.45918502043551 96-97 21.85386787013411 27.478832845794525 28.20263183784073 22.464667446230635 98-99 21.647387528119218 27.503681151508026 28.08265305919132 22.766278261181437 100-101 21.441812841391574 27.781138784274273 28.089656764718118 22.687391609616036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 660.0 1 490.0 2 370.5 3 436.5 4 395.0 5 371.0 6 609.5 7 1339.0 8 2310.0 9 2813.5 10 2549.5 11 2201.0 12 2272.5 13 2482.5 14 2719.0 15 2991.5 16 3207.5 17 3442.5 18 3703.0 19 3954.0 20 4342.0 21 5012.0 22 5874.5 23 7020.0 24 8463.5 25 10393.0 26 13290.0 27 16607.5 28 19444.5 29 22497.5 30 26414.0 31 30321.5 32 34207.5 33 38232.5 34 43556.5 35 48354.5 36 53531.5 37 58851.5 38 61209.5 39 62800.5 40 64074.5 41 65983.5 42 67874.5 43 70154.0 44 72659.5 45 74220.0 46 74721.0 47 75685.5 48 79666.0 49 78914.5 50 73486.5 51 69615.5 52 65854.0 53 62949.0 54 59937.5 55 54988.5 56 49311.5 57 43799.5 58 39039.5 59 34366.0 60 29115.0 61 23527.5 62 18186.0 63 13752.5 64 10060.0 65 7411.0 66 5632.0 67 4242.5 68 2982.0 69 1971.5 70 1299.0 71 850.0 72 591.0 73 414.0 74 279.5 75 200.5 76 133.0 77 70.5 78 44.0 79 24.0 80 11.5 81 10.0 82 6.5 83 3.5 84 3.0 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02087136712435898 2 0.0035864879068110895 3 3.984986563123433E-4 4 0.0 5 0.0022415549417569305 6 0.0 7 4.981233203904291E-5 8 0.0 9 9.962466407808582E-5 10-11 4.981233203904291E-5 12-13 0.005977479844685149 14-15 0.0032128954165182673 16-17 0.0 18-19 7.72091146605165E-4 20-21 0.001046058972819901 22-23 0.0014196514631127227 24-25 4.732171543709076E-4 26-27 2.4906166019521454E-4 28-29 7.471849805856436E-5 30-31 0.0 32-33 9.962466407808582E-5 34-35 0.0 36-37 0.002141930277678845 38-39 0.01028624656606236 40-41 8.468096446637293E-4 42-43 0.0 44-45 0.001320026799034637 46-47 3.237801582537789E-4 48-49 1.2453083009760727E-4 50-51 0.0015690884592298514 52-53 3.4868632427330034E-4 54-55 0.0 56-57 3.4868632427330034E-4 58-59 1.4943699611712872E-4 60-61 0.0017185254553469804 62-63 0.0 64-65 4.981233203904291E-5 66-67 2.4906166019521456E-5 68-69 1.2453083009760727E-4 70-71 3.735924902928218E-4 72-73 1.7434316213665017E-4 74-75 0.002689865930108317 76-77 1.2453083009760727E-4 78-79 2.73967826214736E-4 80-81 0.0017434316213665018 82-83 9.962466407808582E-5 84-85 0.002141930277678845 86-87 2.4906166019521454E-4 88-89 0.0021170241116593236 90-91 0.001320026799034637 92-93 0.0 94-95 0.0 96-97 1.2453083009760727E-4 98-99 5.47935652429472E-4 100-101 0.0017185254553469804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2007535.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.73800403516589 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35572213448715 48.19908171217983 2 12.379608949275346 15.038254766297236 3 3.6864856252035656 6.717293363375856 4 1.5413369547956124 3.7447092071970487 5 0.8192546806736041 2.487994705029095 6 0.4667081437241284 1.7008152670056533 7 0.3167096714351777 1.3465419311124118 8 0.22682313631389983 1.1021427654962106 9 0.16683428248927842 0.9119863190734018 >10 0.9356005987995731 10.45762378969087 >50 0.06985692633350819 2.9175524011232503 >100 0.0334138716022193 3.471613827589511 >500 0.0011515174069138823 0.4629585944054622 >1k 2.4675373005297337E-4 0.2724196843954866 >5k 2.4675373005297337E-4 1.1690116660286791 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9919 0.4940885214952666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8087 0.40283232919974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5394 0.26868771901859745 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2076 0.10341040131305308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.471849805856436E-5 0.0 22-23 0.0 0.0 0.0 2.2415549417569307E-4 0.0 24-25 0.0 0.0 0.0 4.4831098835138613E-4 0.0 26-27 0.0 0.0 0.0 6.226541504880364E-4 0.0 28-29 0.0 0.0 0.0 0.001643806957288416 0.0 30-31 0.0 0.0 0.0 0.0028393029262254454 0.0 32-33 0.0 0.0 0.0 0.004483109883513862 0.0 34-35 0.0 0.0 0.0 0.006052198342743714 0.0 36-37 0.0 0.0 0.0 0.008368471782559207 0.0 38-39 0.0 0.0 0.0 0.012179115183545992 0.0 40-41 0.0 0.0 0.0 0.016363351074825595 0.0 42-43 0.0 0.0 0.0 0.01970077732144147 0.0 44-45 0.0 0.0 0.0 0.021543833606886057 0.0 46-47 0.0 0.0 0.0 0.02386010704670155 0.0 48-49 0.0 0.0 0.0 0.02742168878749312 0.0 50-51 0.0 0.0 0.0 0.031904798671006984 0.0 52-53 0.0 0.0 0.0 0.04094573693609327 0.0 54-55 0.0 0.0 0.0 0.047520964765246934 0.0 56-57 0.0 0.0 0.0 0.0656028412954195 0.0 58-59 0.0 0.0 0.0 0.09130600462756563 0.0 60-61 0.0 0.0 0.0 0.11270040123833458 0.0 62-63 0.0 0.0 0.0 0.12308627246847502 0.0 64-65 0.0 0.0 0.0 0.14413198275497063 0.0 66-67 0.0 0.0 0.0 0.19576246491343863 0.0 68-69 0.0 0.0 0.0 0.274017638546775 0.0 70-71 0.0 0.0 0.0 0.3405171018188973 0.0 72-73 0.0 0.0 0.0 0.4174522486531991 0.0 74-75 0.0 0.0 0.0 0.5069401031613396 0.0 76-77 0.0 0.0 0.0 0.5623812287207944 0.0 78-79 0.0 0.0 0.0 0.6317449010851617 0.0 80-81 0.0 0.0 0.0 0.7394391629535724 0.0 82-83 0.0 0.0 0.0 0.8311436662374504 0.0 84-85 0.0 0.0 0.0 0.9562722443195262 0.0 86-87 0.0 0.0 0.0 1.1501169344494615 0.0 88-89 0.0 0.0 0.0 1.406575725952474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7070 0.0 34.543774 1 GTATCAA 11025 0.0 33.874275 1 ATCAACG 13685 0.0 27.14302 3 TATCAAC 13790 0.0 27.074806 2 TCAACGC 13785 0.0 26.945446 4 CAACGCA 13915 0.0 26.727846 5 AACGCAG 14175 0.0 26.238255 6 ACGCAGA 15970 0.0 23.259377 7 CGCAGAG 16000 0.0 22.980553 8 GAGTACT 9230 0.0 22.18218 12-13 GCAGAGT 16425 0.0 22.06778 9 GTACATG 7630 0.0 21.671091 1 AGTACTT 9595 0.0 21.660164 12-13 AGAGTAC 13400 0.0 21.465887 10-11 GTACTTT 9645 0.0 20.979908 14-15 TACATGG 7870 0.0 20.400885 2 ACATGGG 7700 0.0 19.987133 3 TACTTTT 11065 0.0 18.52361 14-15 GGATCGC 180 1.2338461E-4 18.472334 6 CAGAGTA 16245 0.0 18.057463 10-11 >>END_MODULE