FastQCFastQC Report
Thu 26 May 2016
SRR936815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495234
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61190.4092336049073255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44980.3008224799596585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28190.1885323634962822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36750.045.526091
GTATCAA64750.038.9055141
ATCAACG78350.031.7223223
TCAACGC79150.031.4016934
TATCAAC81300.030.6348512
CAACGCA81150.030.6246975
AACGCAG84500.029.6355196
CGCAGAG96400.025.535278
ACGCAGA97150.025.4831757
GTACATG66950.023.7121491
GCAGAGT102450.023.6562529
GAGTACT51150.023.41104512-13
TACATGG71550.021.8555282
GTACTTT55750.021.5216314-15
ACATGGG69800.021.5146333
AGTACTT54800.020.29089512-13
CAGAGTA100150.019.83312810-11
AGAGTAC93050.019.4569410-11
CATGGGG30700.019.1948874
GCGGTAT1507.688954E-419.0123161
Produced by FastQC (version 0.11.5)