##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936815_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1495234 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.777798658939002 33.0 31.0 34.0 30.0 34.0 2 31.872763059159972 33.0 31.0 34.0 30.0 34.0 3 31.884343186417645 33.0 31.0 34.0 30.0 34.0 4 35.453658089636804 37.0 35.0 37.0 33.0 37.0 5 35.48201886794977 37.0 35.0 37.0 33.0 37.0 6 35.53614150026016 37.0 35.0 37.0 33.0 37.0 7 35.476067959931356 37.0 35.0 37.0 33.0 37.0 8 35.43999333883526 37.0 35.0 37.0 33.0 37.0 9 37.065373045289235 39.0 37.0 39.0 33.0 39.0 10-11 36.93551678198864 39.0 37.0 39.0 33.0 39.0 12-13 36.83513483508267 39.0 37.0 39.0 33.0 39.0 14-15 37.997486012222836 40.0 38.0 41.0 33.0 41.0 16-17 37.92436936292246 40.0 38.0 41.0 33.0 41.0 18-19 37.88450971553617 40.0 38.0 41.0 33.0 41.0 20-21 37.828056009962324 40.0 38.0 41.0 32.5 41.0 22-23 37.699745324143244 40.0 37.5 41.0 32.0 41.0 24-25 37.53191038994565 40.0 37.0 41.0 32.0 41.0 26-27 37.493002767459814 40.0 37.0 41.0 32.0 41.0 28-29 37.56379235624658 40.0 37.0 41.0 32.0 41.0 30-31 37.4296822437157 40.0 37.0 41.0 32.0 41.0 32-33 37.25123659574354 40.0 36.5 41.0 31.0 41.0 34-35 37.06314061879278 40.0 36.0 41.0 31.0 41.0 36-37 36.92539161094518 39.0 36.0 41.0 31.0 41.0 38-39 36.76959559507074 39.0 36.0 41.0 30.0 41.0 40-41 36.562712592142766 39.0 35.5 41.0 30.0 41.0 42-43 36.36186543377157 39.0 35.0 41.0 30.0 41.0 44-45 36.119880567188815 39.0 35.0 40.5 29.5 41.0 46-47 35.774407885320954 39.0 35.0 40.0 28.5 41.0 48-49 35.438811249610424 39.0 35.0 40.0 27.0 41.0 50-51 34.791012309778935 38.5 34.0 39.5 26.0 40.5 52-53 35.10700231535666 38.5 34.5 40.0 27.0 40.5 54-55 35.4207067255025 39.0 35.0 40.5 27.0 41.0 56-57 35.31271760808008 39.0 35.0 41.0 26.5 41.0 58-59 35.08026703512627 38.5 35.0 40.0 26.0 41.0 60-61 34.801157878967445 38.0 34.0 40.0 26.0 41.0 62-63 34.496750341418135 38.0 34.0 40.0 26.0 41.0 64-65 34.1789910475551 37.0 34.0 40.0 25.0 41.0 66-67 33.81060957682878 37.0 33.5 39.5 24.0 41.0 68-69 33.37461427442126 36.0 33.0 39.0 23.0 41.0 70-71 33.01735413988713 36.0 33.0 39.0 22.0 40.5 72-73 32.63777509072159 35.0 32.5 38.5 22.0 40.0 74-75 32.201572462905474 35.0 32.0 37.5 20.5 39.5 76-77 31.761694825024044 35.0 31.5 37.0 19.5 39.0 78-79 31.33344078585693 35.0 31.0 36.5 18.0 39.0 80-81 30.927594610609443 35.0 31.0 36.0 16.5 37.5 82-83 30.527455234431535 34.5 31.0 35.5 12.0 37.0 84-85 30.179913645623362 34.0 30.5 35.0 7.5 37.0 86-87 29.855779095445932 34.0 30.0 35.0 7.0 36.0 88-89 29.48953441401145 34.0 30.0 35.0 2.0 36.0 90-91 29.16114434262463 34.0 29.5 35.0 2.0 36.0 92-93 28.812720617642455 34.0 29.0 35.0 2.0 35.0 94-95 28.433243559202104 34.0 29.0 35.0 2.0 35.0 96-97 27.997076711738764 34.0 29.0 35.0 2.0 35.0 98-99 27.414501007869003 34.0 27.5 35.0 2.0 35.0 100-101 25.266841845490404 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3779939318924974 1101 2 0.2882163096188606 1101 3 0.4707078382435448 1101 4 0.3518160727220305 1101 5 0.17623233092029267 1101 6 0.22959402401564688 1101 7 0.27347248301456517 1101 8 0.1827887148546239 1101 9 0.31916027064733044 1101 10-11 0.3144110797021895 1101 12-13 0.5190964498214896 1101 14-15 0.7012506796994984 1101 16-17 0.7629163601254874 1101 18-19 0.7683279625549275 1101 20-21 0.6921527638465079 1101 22-23 0.7333837146167284 1101 24-25 0.7549727793744694 1101 26-27 0.7992279533816671 1101 28-29 0.8791560094823794 1101 30-31 0.8441901311907785 1101 32-33 0.8165857033999018 1101 34-35 0.8848180275704678 1101 36-37 0.7579921054262613 1101 38-39 0.8433811129486131 1101 40-41 0.9083175803979557 1101 42-43 0.8730343040862749 1101 44-45 0.922482558858043 1101 46-47 1.0442923766426375 1101 48-49 1.0672278147346006 1101 50-51 1.0591212059229207 1101 52-53 1.0391228050829824 1101 54-55 1.0223684352932523 1101 56-57 1.0382593293740854 1101 58-59 1.0550124400632512 1101 60-61 1.045741333362514 1101 62-63 1.0671310761888009 1101 64-65 0.9845120582085727 1101 66-67 1.031857835112703 1101 68-69 1.1111188467702462 1101 70-71 0.9542499955692776 1101 72-73 1.0569313600736123 1101 74-75 1.0474652719210695 1101 76-77 1.1037362369253323 1101 78-79 1.2455797909852926 1101 80-81 1.178458855746129 1101 82-83 0.9227719491366706 1101 84-85 1.0766408934733676 1101 86-87 1.2724602253333828 1101 88-89 1.2134927531642141 1101 90-91 1.159373814797572 1101 92-93 0.9062371726402958 1101 94-95 0.9361421432921198 1101 96-97 0.9946533481488977 1101 98-99 1.279686284715325 1101 100-101 1.5385454221414037 1102 1 0.3116357613396943 1102 2 0.30910969823673895 1102 3 0.23248698108184485 1102 4 0.16418343190432694 1102 5 0.15606044148897524 1102 6 0.18868131234878405 1102 7 0.2333800435905573 1102 8 0.3217660520606742 1102 9 0.17882503185511212 1102 10-11 0.3414818872190395 1102 12-13 0.27554488679105305 1102 14-15 0.273437828200386 1102 16-17 0.24713196985459973 1102 18-19 0.2161671271962433 1102 20-21 0.28323610976707414 1102 22-23 0.2270148279471087 1102 24-25 0.3487418406748546 1102 26-27 0.309905168808136 1102 28-29 0.40694056629983777 1102 30-31 0.2885334894321403 1102 32-33 0.32735622137492015 1102 34-35 0.1670676583871753 1102 36-37 0.2597264083361779 1102 38-39 0.3479863687085185 1102 40-41 0.42529894815253755 1102 42-43 0.30775519118380856 1102 44-45 0.3036786306687844 1102 46-47 0.18490372411682898 1102 48-49 0.3496316834776607 1102 50-51 0.13151668959329754 1102 52-53 0.24646173434050667 1102 54-55 0.37277010732355365 1102 56-57 0.330305575830792 1102 58-59 0.3557493478465972 1102 60-61 0.46558973343654486 1102 62-63 0.5302937408244901 1102 64-65 0.5185366408468255 1102 66-67 0.42634648092194993 1102 68-69 0.23147639915567453 1102 70-71 0.3047834156608147 1102 72-73 0.4220929178432371 1102 74-75 0.5632414564303474 1102 76-77 0.6186640368603555 1102 78-79 0.43314541808326723 1102 80-81 0.5356021687646262 1102 82-83 0.5914029333022874 1102 84-85 0.5612476445053964 1102 86-87 0.6670008409660859 1102 88-89 0.6976080702515546 1102 90-91 0.5914012366293342 1102 92-93 0.6465076466993018 1102 94-95 0.8207709587053387 1102 96-97 0.5225514180018145 1102 98-99 0.49099771433830597 1102 100-101 0.7363693149486323 1103 1 0.18457968941210723 1103 2 0.16757212457778792 1103 3 0.2366206910630133 1103 4 0.14859051531359313 1103 5 0.2253083608134432 1103 6 0.13750459161492756 1103 7 0.2991700927977803 1103 8 0.1296625954055486 1103 9 0.3794996567260185 1103 10-11 0.38121686686790923 1103 12-13 0.06029171262180455 1103 14-15 0.16170494441446692 1103 16-17 0.4378169221661281 1103 18-19 0.5182557001869483 1103 20-21 0.5046451979148188 1103 22-23 0.2784272573256388 1103 24-25 0.48453766109888363 1103 26-27 0.5107377427708357 1103 28-29 0.5461433790086616 1103 30-31 0.6432124275282476 1103 32-33 0.5648949492772388 1103 34-35 0.3437482974734962 1103 36-37 0.5495601061674122 1103 38-39 0.5260926502224024 1103 40-41 0.4875038567243095 1103 42-43 0.5340177207992625 1103 44-45 0.635236403586589 1103 46-47 0.50328641654135 1103 48-49 0.6630614568814721 1103 50-51 0.5978325270272364 1103 52-53 0.5851639699276561 1103 54-55 0.49491259562924483 1103 56-57 0.5461232661290722 1103 58-59 0.4743836956990961 1103 60-61 0.49555434676502585 1103 62-63 0.45850375236692287 1103 64-65 0.5483218908316374 1103 66-67 0.5766822746161324 1103 68-69 0.7705842905353038 1103 70-71 0.6495486182609014 1103 72-73 0.6346143320951043 1103 74-75 0.568175543059354 1103 76-77 0.38244555074164666 1103 78-79 0.4202753008364404 1103 80-81 0.5139628394407723 1103 82-83 0.5089187282114658 1103 84-85 0.4148324813925832 1103 86-87 0.5896197584073128 1103 88-89 0.6622788071448191 1103 90-91 0.5764234102518344 1103 92-93 0.4941715188820339 1103 94-95 0.5298455896512131 1103 96-97 0.49189456240373275 1103 98-99 0.49097311072977945 1103 100-101 0.8458754202885252 1104 1 0.5297994660001208 1104 2 0.6396885896448765 1104 3 0.48564541179802845 1104 4 0.5356997095762708 1104 5 0.5951801302628468 1104 6 0.5156115385380602 1104 7 0.5285752088001558 1104 8 0.5724304584500359 1104 9 0.6445108934237496 1104 10-11 0.6103366037969806 1104 12-13 0.6738413523864963 1104 14-15 0.8345131273743362 1104 16-17 0.7249155407132903 1104 18-19 0.7575299417098478 1104 20-21 0.6089326590821145 1104 22-23 0.7769598767981734 1104 24-25 0.7483809422146166 1104 26-27 0.77740804483971 1104 28-29 0.7731968855193401 1104 30-31 0.9234074172830233 1104 32-33 0.9528832498919471 1104 34-35 0.9117131332194219 1104 36-37 0.9596319879837907 1104 38-39 0.9127264330236429 1104 40-41 0.9733591677164455 1104 42-43 0.9757245421357723 1104 44-45 0.9802359078499308 1104 46-47 1.006021065229298 1104 48-49 0.8821798718461125 1104 50-51 1.0241511865552937 1104 52-53 1.231185022211342 1104 54-55 1.2401675695204446 1104 56-57 1.1650884984643284 1104 58-59 1.2513511092756673 1104 60-61 1.167821020041508 1104 62-63 1.1340219624270773 1104 64-65 1.1307295185664472 1104 66-67 1.0335169960121249 1104 68-69 1.0540336262481702 1104 70-71 1.1360302099060888 1104 72-73 1.024591528208525 1104 74-75 0.947309656351706 1104 76-77 0.9265451277130232 1104 78-79 1.048181639655926 1104 80-81 1.1608823318064267 1104 82-83 1.102381779305393 1104 84-85 1.1573111647160026 1104 86-87 1.198584660251452 1104 88-89 1.2374916434195207 1104 90-91 1.2192583629916065 1104 92-93 1.0964482721373727 1104 94-95 1.1621314314120674 1104 96-97 1.0791067046471063 1104 98-99 1.1926417603431005 1104 100-101 1.2643683808278894 1105 1 0.24222183217138848 1105 2 0.35987928994290286 1105 3 0.26982318455527476 1105 4 0.19069559820166404 1105 5 0.22147084882549706 1105 6 0.11411508620322763 1105 7 0.14754247257582875 1105 8 0.10201291736746043 1105 9 0.23338443546283116 1105 10-11 0.26488963026015 1105 12-13 0.3401132635191857 1105 14-15 0.5238593491274344 1105 16-17 0.5497110172476454 1105 18-19 0.561190348768946 1105 20-21 0.5099150979011142 1105 22-23 0.5201453008932475 1105 24-25 0.6070866404111186 1105 26-27 0.48078226941832014 1105 28-29 0.6191831789812667 1105 30-31 0.337406152696083 1105 32-33 0.4008485045587804 1105 34-35 0.36158498743102996 1105 36-37 0.34703955540703646 1105 38-39 0.4467129406731516 1105 40-41 0.49848549804503506 1105 42-43 0.5759834010294327 1105 44-45 0.43766447592527413 1105 46-47 0.39910056916499315 1105 48-49 0.43461475185338116 1105 50-51 0.3526311295755278 1105 52-53 0.4104451728321621 1105 54-55 0.4304636985200503 1105 56-57 0.2719521467976591 1105 58-59 0.37949946498255827 1105 60-61 0.24276595109437693 1105 62-63 0.15786188361778386 1105 64-65 0.22229709291411126 1105 66-67 0.3000043050312584 1105 68-69 0.26389912818429195 1105 70-71 0.35274494652504274 1105 72-73 0.20625633784931807 1105 74-75 0.23933186584329746 1105 76-77 0.12267126659313377 1105 78-79 0.2770289657220424 1105 80-81 0.2715111337276568 1105 82-83 0.4314872307460611 1105 84-85 0.42784933301609485 1105 86-87 0.5936518175935035 1105 88-89 0.5601408430401484 1105 90-91 0.6223817607108018 1105 92-93 0.7201195939608134 1105 94-95 0.8458721001320981 1105 96-97 0.7976429525941704 1105 98-99 0.7214355028382862 1105 100-101 0.9227393742548209 1106 1 0.6114217019242218 1106 2 0.544226455355826 1106 3 0.5412814541258406 1106 4 0.3570736415802287 1106 5 0.3925468840313471 1106 6 0.2695673284199316 1106 7 0.3999076027105417 1106 8 0.4154089166673316 1106 9 0.5835048554219355 1106 10-11 0.6457161619599816 1106 12-13 0.695549664864302 1106 14-15 0.9122657867794288 1106 16-17 0.8143007533406816 1106 18-19 0.737383376634213 1106 20-21 0.6491922945753146 1106 22-23 0.8280175295963161 1106 24-25 0.9269541735669478 1106 26-27 0.9236688380215199 1106 28-29 0.707412433550914 1106 30-31 0.7242918177828983 1106 32-33 0.8691838734115223 1106 34-35 0.8962586508085906 1106 36-37 0.9273403070661672 1106 38-39 1.0072568497113465 1106 40-41 0.7532898290566763 1106 42-43 0.9738743160940047 1106 44-45 1.0614014842104353 1106 46-47 0.7914547573643276 1106 48-49 1.049544186637398 1106 50-51 1.0678100580872396 1106 52-53 0.9576287245315811 1106 54-55 1.1355604500657606 1106 56-57 1.2111884701118072 1106 58-59 1.1865556932319166 1106 60-61 1.1104342921346557 1106 62-63 1.1727618367796495 1106 64-65 1.2181029288085483 1106 66-67 1.3355951744310914 1106 68-69 1.3531206373499955 1106 70-71 1.2943085090001674 1106 72-73 1.1955593492772323 1106 74-75 1.2629081186166644 1106 76-77 1.3062743021734882 1106 78-79 1.369583169371115 1106 80-81 1.5376517107069674 1106 82-83 1.5436753146948625 1106 84-85 1.5998496635231696 1106 86-87 1.5664397425468835 1106 88-89 1.5347340895485537 1106 90-91 1.5536416673669997 1106 92-93 1.6043966488494341 1106 94-95 1.643186192347482 1106 96-97 1.691011885515902 1106 98-99 1.8152435310311255 1106 100-101 2.047371587351705 1107 1 0.07723281345027289 1107 2 0.22909874815427145 1107 3 0.25165454763426354 1107 4 0.15107589543055866 1107 5 0.17167339620894495 1107 6 0.20130268333545587 1107 7 0.40036122853184963 1107 8 0.39995653200142556 1107 9 0.25415493527326305 1107 10-11 0.3840800555413395 1107 12-13 0.2522869419231313 1107 14-15 0.14912107245385897 1107 16-17 0.3526260942190689 1107 18-19 0.2587543151453957 1107 20-21 0.28968367129124317 1107 22-23 0.44576336074207035 1107 24-25 0.1687820439859209 1107 26-27 0.3274444217489858 1107 28-29 0.328997334071758 1107 30-31 0.43335400955349 1107 32-33 0.4844471191756625 1107 34-35 0.46138141535592325 1107 36-37 0.4176510572292784 1107 38-39 0.48176593430981285 1107 40-41 0.7379362974753079 1107 42-43 0.7183228916210496 1107 44-45 0.9275332641590808 1107 46-47 0.6279547760365745 1107 48-49 0.8198612414305657 1107 50-51 0.7003717698711895 1107 52-53 0.8155740961203222 1107 54-55 0.7943778156969543 1107 56-57 0.8173067071878677 1107 58-59 0.7653778477458815 1107 60-61 0.845245790286107 1107 62-63 0.7824080306063905 1107 64-65 0.8543298932939294 1107 66-67 0.5794223484850107 1107 68-69 0.6160228870265314 1107 70-71 0.6489730565496004 1107 72-73 0.5784555139972269 1107 74-75 0.8148729884271546 1107 76-77 0.6212213279038501 1107 78-79 0.667372869319049 1107 80-81 0.8851447415645026 1107 82-83 1.0299105718556625 1107 84-85 0.962773386286397 1107 86-87 0.9947998138089744 1107 88-89 1.0481267603611926 1107 90-91 0.8493381532496791 1107 92-93 1.1143069451670442 1107 94-95 1.0905727364656137 1107 96-97 1.0759304195905592 1107 98-99 1.0495742187630164 1107 100-101 1.1494395938804018 1108 1 0.08213180632592554 1108 2 0.17446015380623692 1108 3 0.08869784558697802 1108 4 0.16412350478275783 1108 5 0.021145455161175164 1108 6 0.03728107990205842 1108 7 0.09332225269461958 1108 8 -0.06318180605587287 1108 9 -0.0473787013225504 1108 10-11 -0.01060722511576273 1108 12-13 -0.010926318755792863 1108 14-15 0.2587724277585437 1108 16-17 0.155817137081236 1108 18-19 0.20982243132814915 1108 20-21 0.2874960468295029 1108 22-23 0.22665420768024802 1108 24-25 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54-55 -1.7733657763255977 2316 56-57 -1.4869872508049724 2316 58-59 -1.5473156507061319 2316 60-61 -1.2067584874595738 2316 62-63 -1.0416715298671733 2316 64-65 -1.3975396659122765 2316 66-67 -1.575594908984577 2316 68-69 -0.8261269394112176 2316 70-71 -1.5617050953457579 2316 72-73 -1.4015977891170195 2316 74-75 -1.546595698770716 2316 76-77 -2.1015547463468813 2316 78-79 -2.1652475749805546 2316 80-81 -1.904269483791552 2316 82-83 -1.8291279146881188 2316 84-85 -1.6015549220536442 2316 86-87 -1.6558882629830585 2316 88-89 -2.010528679486196 2316 90-91 -2.0586627454225557 2316 92-93 -2.4679110717299686 2316 94-95 -2.54114817148961 2316 96-97 -2.3386595077091634 2316 98-99 -2.309849824504923 2316 100-101 -2.101682511975291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4012.0 3 1142.0 4 1185.0 5 1196.0 6 1358.0 7 1628.0 8 1949.0 9 2433.0 10 3167.0 11 3722.0 12 4751.0 13 5957.0 14 6516.0 15 8202.0 16 10268.0 17 13635.0 18 12058.0 19 7838.0 20 7560.0 21 8136.0 22 8876.0 23 9743.0 24 11088.0 25 12953.0 26 15209.0 27 18066.0 28 21441.0 29 26365.0 30 32364.0 31 41118.0 32 52342.0 33 69109.0 34 94741.0 35 135930.0 36 206251.0 37 306127.0 38 277178.0 39 49588.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91400543689944 19.080813313042 14.39158793067217 22.61359331938639 2 15.268099602041746 20.49009657048896 40.62200393137764 23.619799896091653 3 19.099014924054515 25.185563463708537 29.7918878145878 25.92353379764915 4 12.705105243487335 16.947798212206987 37.63092643471528 32.7161701095904 5 13.806783903278792 37.37099475240836 34.046939730703535 14.77528161360932 6 31.189347202823893 36.40031549162997 17.99554347986197 14.41479382568418 7 28.34112229325551 32.953092805250996 20.873430461518474 17.83235443997501 8 26.16405770359921 34.732829282695285 20.074208322928055 19.028904690777455 9 26.556450916221163 16.999530555103522 20.55304760814142 35.890970920533896 10-11 24.56780120663448 27.05512886615196 27.45337850958545 20.923691417628113 12-13 25.786070887475958 24.456018526267304 27.931333908652757 21.826576677603985 14-15 22.83793157009566 25.608590159719768 26.428312230185004 25.12516603999957 16-17 22.00555060583567 29.11356041331221 26.73782879504845 22.143060185803666 18-19 21.852111334752394 27.410748327237222 29.467638161719982 21.269502176290402 20-21 22.737664422216742 26.5321893209521 29.436475619008668 21.29367063782249 22-23 22.37809373777373 26.59733440966341 29.247161330030714 21.777410522532147 24-25 22.24187671147154 26.800201185225937 29.358988130674017 21.598933972628505 26-27 21.958388264660808 27.057967328965592 29.15692402411848 21.826720382255118 28-29 21.75621222409218 27.013223094584966 29.409329622268533 21.82123505905432 30-31 21.929251370195534 26.87547238747768 29.371250803414206 21.824025438912585 32-33 21.805019195213006 26.97568160237369 29.24996470115091 21.969334501262395 34-35 22.230340988169797 27.044289652588194 29.016748300412182 21.708621058829827 36-37 22.182510711735702 26.809819933096403 28.94654604657823 22.061123308589664 38-39 21.93965414845525 27.0528808303435 29.146205138688924 21.861259882512325 40-41 22.15969156251056 26.874901906584615 29.109139445081578 21.856267085823248 42-43 21.982523796201978 26.949675600644113 28.901927924044905 22.165872679109004 44-45 22.172267275360834 27.155348508528643 28.650584051289307 22.02180016482121 46-47 22.30196260134587 27.248795224052362 28.220678488330275 22.228563686271492 48-49 22.364681372278415 27.469155318948907 27.94768825066654 22.218475058106137 50-51 22.280726577936733 27.5954241498889 27.883129103291125 22.240720168883243 52-53 22.54104352649633 27.514042505071163 27.81595343666244 22.12896053177007 54-55 22.279915310605848 27.480155941975454 27.837288761658506 22.40263998576019 56-57 22.309796647099606 27.97430718949 27.633891168589912 22.082004994820483 58-59 22.470530287516247 27.562027939174673 27.67191613696808 22.295525636341004 60-61 22.498770339587036 27.43176639463567 28.069717562896713 21.999745702880585 62-63 22.293729610383263 27.530169689129142 28.1684489786494 22.007651721838194 64-65 22.230188292931636 27.462926546285697 27.958499410852756 22.34838574992991 66-67 22.136906702040722 27.860650399865605 27.627638078958483 22.37480481913519 68-69 22.069447813757392 27.72903474063726 27.9094217143622 22.29209573124315 70-71 22.11234844085748 27.685583852244722 27.883804880398394 22.318262826499403 72-73 22.180341604467316 27.630861135593193 27.69618508787627 22.49261217206322 74-75 22.11184745088581 27.927809190506093 27.518966110715454 22.441377247892643 76-77 22.25476032912966 27.832393322926197 27.63152171949304 22.281324628451102 78-79 22.254790413150126 27.820303454184042 27.469162656529218 22.455743476136615 80-81 22.094310901456986 28.04389354477175 27.480035462772452 22.38176009099881 82-83 22.252063205030865 27.984130813707388 27.46770631966653 22.29609966159522 84-85 22.221300111467574 27.767171645005057 27.37340432545051 22.638123918076854 86-87 22.227308853918295 27.656618895832814 27.627437692944806 22.488634557304085 88-89 22.28279661564426 27.62018282108417 27.492919691341722 22.604100871929845 90-91 22.108608493210003 27.776861238171758 27.735332512574907 22.379197756043332 92-93 22.15806766104749 27.63300794565322 27.66393243734862 22.54499195595067 94-95 22.005461108180548 27.64510294302721 27.598444541435608 22.75099140735663 96-97 22.15671349017938 27.659112241195317 27.51564242743159 22.668531841193712 98-99 21.958837831584226 27.671869281334644 27.397590998877234 22.971701888203892 100-101 21.925092326170805 27.629341188438477 27.607241171126795 22.838325314263923 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 416.0 1 303.0 2 217.5 3 258.0 4 255.0 5 286.5 6 439.5 7 807.0 8 1336.0 9 1597.0 10 1557.0 11 1503.5 12 1564.5 13 1692.5 14 1836.0 15 1977.5 16 2030.5 17 2128.0 18 2226.5 19 2383.0 20 2704.0 21 3144.5 22 3684.5 23 4412.0 24 5220.5 25 6457.5 26 8409.5 27 10588.5 28 12541.5 29 14534.5 30 17155.5 31 20421.0 32 23748.0 33 27010.0 34 31219.5 35 34556.0 36 38137.0 37 42220.5 38 44052.0 39 44620.5 40 45359.5 41 47406.5 42 49373.5 43 51428.5 44 53623.0 45 55065.5 46 55138.5 47 55354.0 48 58159.5 49 58639.5 50 56545.5 51 54455.5 52 51838.0 53 50716.5 54 49121.0 55 44768.0 56 40171.0 57 36276.0 58 32876.0 59 29068.5 60 24338.5 61 19324.5 62 14840.5 63 11384.5 64 8547.0 65 6377.0 66 4602.5 67 3456.0 68 2447.5 69 1542.0 70 1050.0 71 728.0 72 534.0 73 370.5 74 271.5 75 208.0 76 133.5 77 70.0 78 41.0 79 26.5 80 22.0 81 17.0 82 16.5 83 13.0 84 8.5 85 8.0 86 6.5 87 4.5 88 4.5 89 3.0 90 0.5 91 0.5 92 0.0 93 1.5 94 1.5 95 1.0 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06687916406395253 2 0.10767545414296358 3 0.07597473037665008 4 0.14847174422197462 5 0.11951306618228318 6 0.1131595455962077 7 0.1257997076042947 8 0.107073541666388 9 0.13248762401068997 10-11 0.12198759525264942 12-13 0.10506716674446943 14-15 0.1082104874554752 16-17 0.1259669055144546 18-19 0.10182352728736774 20-21 0.08376615299010054 22-23 0.08483621961512379 24-25 0.07279797008361233 26-27 0.06313393087637119 28-29 0.07647632410712972 30-31 0.05554314575511258 32-33 0.056914168618423605 34-35 0.05042688970422021 36-37 0.05651289363403989 38-39 0.05781703733328696 40-41 0.07507186166178671 42-43 0.07276453050158035 44-45 0.059355258106757874 46-47 0.0442405670283046 48-49 0.05153039591127542 50-51 0.031667284184281524 52-53 0.05039345012218823 54-55 0.0556434645012085 56-57 0.059254939360661944 58-59 0.0660766140951851 60-61 0.06146195177477238 62-63 0.07092535348982167 64-65 0.046882294008830726 66-67 0.07403523461879545 68-69 0.06330112878653107 70-71 0.029293073860011207 72-73 0.02518000527007813 74-75 0.035345638207798916 76-77 0.04995873555577254 78-79 0.07383459712660359 80-81 0.047417327321342344 82-83 0.0680495494350717 84-85 0.07206229927890885 86-87 0.07500498249772276 88-89 0.09888084406855382 90-91 0.07273109091954838 92-93 0.0851037362713796 94-95 0.09349707136140564 96-97 0.1029939126584869 98-99 0.13660069260062305 100-101 0.135263109319344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1495234.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.83069308943245 #Duplication Level Percentage of deduplicated Percentage of total 1 81.62575035267395 49.65350967898141 2 10.974507739226661 13.351738241849965 3 3.1287280076579305 5.709680795824531 4 1.2979100242994261 3.158110653834248 5 0.7060921939350854 2.14760387710546 6 0.44124890706311054 1.6104886104962148 7 0.29643632864596736 1.262269912989468 8 0.21213846363955552 1.032362381929722 9 0.16175686196357325 0.8855803822694233 >10 1.0220331889201075 11.717905127806665 >50 0.08816038815097728 3.6707184384385805 >100 0.04413422506491847 4.5733592586075265 >500 6.619912552386347E-4 0.24175055254144154 >1k 3.3099562761931735E-4 0.574241456841914 >5k 1.103318758731058E-4 0.41068063048336506 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6119 0.4092336049073255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4498 0.3008224799596585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2819 0.1885323634962822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.687916406395254E-5 0.0 16-17 0.0 0.0 0.0 1.6719791015988133E-4 0.0 18-19 0.0 0.0 0.0 2.6751665625581014E-4 0.0 20-21 0.0 0.0 0.0 2.6751665625581014E-4 0.0 22-23 0.0 0.0 0.0 6.019124765755729E-4 0.0 24-25 0.0 0.0 0.0 6.687916406395253E-4 0.0 26-27 0.0 0.0 0.0 0.001003187460959288 6.687916406395254E-5 28-29 0.0 0.0 0.0 0.0020063749219185758 6.687916406395254E-5 30-31 0.0 0.0 0.0 0.004547783156348772 6.687916406395254E-5 32-33 0.0 0.0 0.0 0.007122630972810944 1.3375832812790507E-4 34-35 0.0 0.0 0.0 0.009630599625209166 1.3375832812790507E-4 36-37 0.0 0.0 0.0 0.012406084933863195 1.3375832812790507E-4 38-39 0.0 0.0 0.0 0.017321703492563706 1.3375832812790507E-4 40-41 0.0 0.0 0.0 0.022170442887200263 1.3375832812790507E-4 42-43 0.0 3.343958203197627E-5 0.0 0.030797855051450142 1.3375832812790507E-4 44-45 0.0 6.687916406395254E-5 0.0 0.03802080477035701 1.3375832812790507E-4 46-47 3.343958203197627E-5 6.687916406395254E-5 0.0 0.04985841680967661 1.3375832812790507E-4 48-49 6.687916406395254E-5 6.687916406395254E-5 0.0 0.06363552460685083 1.3375832812790507E-4 50-51 6.687916406395254E-5 6.687916406395254E-5 0.0 0.06911961606009494 1.3375832812790507E-4 52-53 6.687916406395254E-5 6.687916406395254E-5 0.0 0.08055595311503083 1.3375832812790507E-4 54-55 6.687916406395254E-5 6.687916406395254E-5 0.0 0.10386334179131829 1.3375832812790507E-4 56-57 6.687916406395254E-5 6.687916406395254E-5 0.0 0.13950993623740499 1.3375832812790507E-4 58-59 6.687916406395254E-5 6.687916406395254E-5 0.0 0.17529028901161958 1.3375832812790507E-4 60-61 6.687916406395254E-5 6.687916406395254E-5 0.0 0.22381112254001717 1.3375832812790507E-4 62-63 6.687916406395254E-5 6.687916406395254E-5 0.0 0.27049277905665603 1.3375832812790507E-4 64-65 6.687916406395254E-5 6.687916406395254E-5 0.0 0.31556933563576006 1.3375832812790507E-4 66-67 6.687916406395254E-5 6.687916406395254E-5 0.0 0.38796603073498864 1.3375832812790507E-4 68-69 6.687916406395254E-5 6.687916406395254E-5 0.0 0.48136278335029836 1.3375832812790507E-4 70-71 6.687916406395254E-5 6.687916406395254E-5 0.0 0.532592223023286 1.3375832812790507E-4 72-73 6.687916406395254E-5 6.687916406395254E-5 0.0 0.5985685183723752 1.6719791015988133E-4 74-75 6.687916406395254E-5 6.687916406395254E-5 0.0 0.6763155465967199 2.006374921918576E-4 76-77 6.687916406395254E-5 6.687916406395254E-5 0.0 0.7458029980591667 2.006374921918576E-4 78-79 6.687916406395254E-5 6.687916406395254E-5 0.0 0.8220786846741046 2.006374921918576E-4 80-81 6.687916406395254E-5 6.687916406395254E-5 0.0 0.9633609187592043 2.006374921918576E-4 82-83 6.687916406395254E-5 6.687916406395254E-5 0.0 1.125843847852577 2.006374921918576E-4 84-85 6.687916406395254E-5 6.687916406395254E-5 0.0 1.3532998848340796 2.006374921918576E-4 86-87 6.687916406395254E-5 6.687916406395254E-5 0.0 1.6702402433331505 2.006374921918576E-4 88-89 6.687916406395254E-5 6.687916406395254E-5 0.0 2.005070778219329 2.006374921918576E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3675 0.0 45.52609 1 GTATCAA 6475 0.0 38.905514 1 ATCAACG 7835 0.0 31.722322 3 TCAACGC 7915 0.0 31.401693 4 TATCAAC 8130 0.0 30.634851 2 CAACGCA 8115 0.0 30.624697 5 AACGCAG 8450 0.0 29.635519 6 CGCAGAG 9640 0.0 25.53527 8 ACGCAGA 9715 0.0 25.483175 7 GTACATG 6695 0.0 23.712149 1 GCAGAGT 10245 0.0 23.656252 9 GAGTACT 5115 0.0 23.411045 12-13 TACATGG 7155 0.0 21.855528 2 GTACTTT 5575 0.0 21.52163 14-15 ACATGGG 6980 0.0 21.514633 3 AGTACTT 5480 0.0 20.290895 12-13 CAGAGTA 10015 0.0 19.833128 10-11 AGAGTAC 9305 0.0 19.45694 10-11 CATGGGG 3070 0.0 19.194887 4 GCGGTAT 150 7.688954E-4 19.012316 1 >>END_MODULE