FastQCFastQC Report
Thu 26 May 2016
SRR936815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495234
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83080.5556320950433177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65160.43578463304071474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43640.29186067197508886No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17400.11636974547127742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.049.4914931
GTATCAA73700.041.701631
ATCAACG95350.031.8809993
TATCAAC96450.031.5666482
TCAACGC97000.031.3386964
CAACGCA98700.030.7978885
AACGCAG101750.029.9690726
ACGCAGA114150.026.3390717
CGCAGAG114950.025.990488
GCAGAGT119450.024.6534799
GTACATG68850.023.9409711
TACATGG70800.023.0108952
ACATGGG70900.022.4425013
GAGTACT72050.022.31465112-13
GTGGTAT22650.021.3918421
AGAGTAC110750.021.16489210-11
GTACTTT76550.020.97185714-15
AGTACTT75800.020.80339812-13
TGGTATC22850.020.5788762
CAGAGTA117750.020.18904910-11
Produced by FastQC (version 0.11.5)