##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936815_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1495234 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18965258949435 33.0 31.0 34.0 31.0 34.0 2 32.18888615427418 33.0 31.0 34.0 30.0 34.0 3 32.365497306776064 34.0 31.0 34.0 31.0 34.0 4 36.09061658576517 37.0 35.0 37.0 35.0 37.0 5 36.020844897855454 37.0 35.0 37.0 35.0 37.0 6 36.07033347288785 37.0 35.0 37.0 35.0 37.0 7 36.012763888461606 37.0 35.0 37.0 35.0 37.0 8 35.990198858506425 37.0 35.0 37.0 35.0 37.0 9 37.69245817042684 39.0 37.0 39.0 35.0 39.0 10-11 37.623413459030495 39.0 37.0 39.0 35.0 39.0 12-13 37.527381667351065 39.0 37.0 39.0 35.0 39.0 14-15 38.75163853951957 40.0 38.0 41.0 35.0 41.0 16-17 38.66717015530679 40.0 38.0 41.0 34.5 41.0 18-19 38.733585177972145 40.0 38.0 41.0 34.5 41.0 20-21 38.81830402465434 40.0 38.0 41.0 35.0 41.0 22-23 38.752132776542 40.0 38.0 41.0 34.5 41.0 24-25 38.654804866663014 40.0 38.0 41.0 34.0 41.0 26-27 38.501916088050436 40.0 38.0 41.0 34.0 41.0 28-29 38.323370121332175 40.0 38.0 41.0 34.0 41.0 30-31 38.18039851956282 40.0 38.0 41.0 34.0 41.0 32-33 38.161378419698856 40.0 37.5 41.0 34.0 41.0 34-35 38.071623237566826 40.0 37.0 41.0 33.5 41.0 36-37 37.90641531693367 40.0 37.0 41.0 33.0 41.0 38-39 37.75385959655813 40.0 37.0 41.0 33.0 41.0 40-41 37.572170977920514 40.0 37.0 41.0 33.0 41.0 42-43 37.345399449183205 40.0 37.0 41.0 32.5 41.0 44-45 36.970626336747294 40.0 36.0 41.0 31.0 41.0 46-47 36.65699214972372 40.0 36.0 41.0 31.0 41.0 48-49 36.296672627829494 39.0 36.0 41.0 30.0 41.0 50-51 36.02616078821107 39.0 35.5 41.0 29.5 41.0 52-53 35.77586651988919 39.0 35.0 40.0 28.5 41.0 54-55 35.53866184155791 39.0 35.0 40.0 28.0 41.0 56-57 35.275530452089775 38.0 35.0 40.0 27.5 41.0 58-59 34.95323273815336 38.0 34.0 40.0 27.0 41.0 60-61 34.63091061332206 38.0 34.0 40.0 26.0 41.0 62-63 34.26559722424717 37.0 34.0 40.0 26.0 41.0 64-65 33.873368315594746 37.0 33.0 39.5 25.0 41.0 66-67 33.50771852432462 36.0 33.0 39.0 24.5 40.0 68-69 33.07529055652827 36.0 32.5 39.0 23.0 40.0 70-71 32.606642505454 35.0 32.0 38.0 22.0 40.0 72-73 32.272811145278936 35.0 32.0 37.5 22.0 39.5 74-75 31.82687759909151 35.0 31.0 37.0 20.5 39.0 76-77 30.434600203045143 33.5 29.5 35.5 19.5 38.0 78-79 31.14478536469877 34.0 31.0 36.0 20.0 38.5 80-81 30.979235691537244 34.0 31.0 36.0 18.5 37.5 82-83 30.652421962047413 34.0 31.0 35.0 18.0 37.0 84-85 30.276474785886357 34.0 31.0 35.0 14.5 36.5 86-87 29.928300185790317 34.0 30.0 35.0 8.5 36.0 88-89 29.581942358186076 34.0 30.0 35.0 6.5 36.0 90-91 29.179860142292107 34.0 29.5 35.0 2.0 35.0 92-93 28.742097223578384 34.0 29.0 35.0 2.0 35.0 94-95 28.29134837757836 34.0 29.0 35.0 2.0 35.0 96-97 27.682137377828486 33.0 28.0 35.0 2.0 35.0 98-99 26.98205765786492 33.0 26.5 35.0 2.0 35.0 100-101 23.981691494441673 29.5 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3303669964109872 1101 2 0.1778550353517332 1101 3 0.24999531902658845 1101 4 0.17763810309462968 1101 5 0.26274704044127617 1101 6 0.1790291361956804 1101 7 0.2753628308581213 1101 8 0.25612798568328543 1101 9 0.3971192810198261 1101 10-11 0.357066859439108 1101 12-13 0.3867906063010338 1101 14-15 0.4697689428063043 1101 16-17 0.45935163923746103 1101 18-19 0.3841880593845701 1101 20-21 0.3367982333089685 1101 22-23 0.3911685141340868 1101 24-25 0.40506315403656146 1101 26-27 0.409630270668238 1101 28-29 0.43567894233706284 1101 30-31 0.39118459922013926 1101 32-33 0.5234762682845613 1101 34-35 0.5364967073640088 1101 36-37 0.5410896318843896 1101 38-39 0.5718354383535384 1101 40-41 0.6117367018311839 1101 42-43 0.6276408850954169 1101 44-45 0.7496123803879655 1101 46-47 0.687756260050918 1101 48-49 0.45266945895396304 1101 50-51 0.6028342761419481 1101 52-53 0.4800463173287426 1101 54-55 0.5389302853551783 1101 56-57 0.7766263565941571 1101 58-59 0.7245164564119193 1101 60-61 0.6686193552279107 1101 62-63 0.7184904207016061 1101 64-65 0.8179609469988591 1101 66-67 0.9187668594126563 1101 68-69 0.7087874751660976 1101 70-71 0.7026438476910712 1101 72-73 0.8694670162501481 1101 74-75 0.8823941923179 1101 76-77 0.6951429467169419 1101 78-79 0.6301202329229341 1101 80-81 0.8185430818934272 1101 82-83 0.993480440370643 1101 84-85 0.9624711096167466 1101 86-87 0.8061947784787868 1101 88-89 0.7726488728871495 1101 90-91 0.8071199277911809 1101 92-93 0.9637229036925063 1101 94-95 0.9360520416576925 1101 96-97 1.1787684711846111 1101 98-99 1.2282408037178314 1101 100-101 1.2673619170795725 1102 1 0.15799129774357112 1102 2 0.023022343232817377 1102 3 0.11766799180421827 1102 4 0.12467416680902943 1102 5 0.1204536637977256 1102 6 0.17622033439187135 1102 7 0.1685624123034941 1102 8 0.2537838731196871 1102 9 0.29074890515643403 1102 10-11 0.2403914146336561 1102 12-13 0.30883906223503743 1102 14-15 0.40669079751508974 1102 16-17 0.33850143723896053 1102 18-19 0.28367690122033196 1102 20-21 0.31312734634818895 1102 22-23 0.28509840836182576 1102 24-25 0.36070326270515807 1102 26-27 0.2990014084517938 1102 28-29 0.17668261639084193 1102 30-31 0.22659039388585 1102 32-33 0.3052454061720553 1102 34-35 0.3124572297488655 1102 36-37 0.41250443586849883 1102 38-39 0.42730918280363284 1102 40-41 0.26829169213470294 1102 42-43 0.35322456547675074 1102 44-45 0.4305700900201046 1102 46-47 0.45329029861374437 1102 48-49 0.3977059001333956 1102 50-51 0.402223007686203 1102 52-53 0.4581882958472647 1102 54-55 0.4059977072355707 1102 56-57 0.3024463933012669 1102 58-59 0.35693739009994374 1102 60-61 0.243571541470736 1102 62-63 0.3871578749187776 1102 64-65 0.48998455771023686 1102 66-67 0.5107124181353626 1102 68-69 0.4458409867609916 1102 70-71 0.3966727277170605 1102 72-73 0.39832956700809774 1102 74-75 0.22477017362821172 1102 76-77 0.37384650324597857 1102 78-79 0.5000972051245505 1102 80-81 0.4300998030048149 1102 82-83 0.3539723119559426 1102 84-85 0.22269799588580597 1102 86-87 0.2429297241733579 1102 88-89 0.46480471184877814 1102 90-91 0.5013477215566837 1102 92-93 0.3890676444527408 1102 94-95 0.5494457896575362 1102 96-97 0.6898281662767474 1102 98-99 0.7122970089299479 1102 100-101 0.9416310192568034 1103 1 -0.0015825434568412788 1103 2 -0.14288920527577886 1103 3 -0.016449619519519842 1103 4 0.08915900977117985 1103 5 0.14426084440643905 1103 6 0.1544525195568056 1103 7 0.23798620186670405 1103 8 0.20710414527811594 1103 9 0.1087386992151167 1103 10-11 0.1959761300215419 1103 12-13 0.24101146974368248 1103 14-15 0.2771602404408 1103 16-17 0.3340176573956981 1103 18-19 0.2102466721941454 1103 20-21 0.171718713822834 1103 22-23 0.22942773221820545 1103 24-25 0.139755112532022 1103 26-27 0.3744017486498379 1103 28-29 0.326647381593439 1103 30-31 0.26086899178391576 1103 32-33 0.41700137482523303 1103 34-35 0.3414454443166406 1103 36-37 0.417789959226738 1103 38-39 0.46416250277204796 1103 40-41 0.39637261066942386 1103 42-43 0.40017037678833134 1103 44-45 0.6125562998494019 1103 46-47 0.6139814777644617 1103 48-49 0.5628356006376123 1103 50-51 0.486122831512219 1103 52-53 0.6068800521196707 1103 54-55 0.5616963282184955 1103 56-57 0.4891212611707729 1103 58-59 0.5650016024807201 1103 60-61 0.5262598653878783 1103 62-63 0.5276939905861084 1103 64-65 0.40955596892462154 1103 66-67 0.5516033117627259 1103 68-69 0.6139276765125317 1103 70-71 0.4133267888312062 1103 72-73 0.4521760830156296 1103 74-75 0.46730540842561297 1103 76-77 0.6009364431038229 1103 78-79 0.5499940825925016 1103 80-81 0.5647845123177362 1103 82-83 0.47452376691007814 1103 84-85 0.5365258313757515 1103 86-87 0.3966353009912922 1103 88-89 0.5177498549142037 1103 90-91 0.8188188654726041 1103 92-93 0.6036294875971429 1103 94-95 0.5954019891593916 1103 96-97 0.842763437523935 1103 98-99 1.1462007814832553 1103 100-101 1.0191904020403513 1104 1 0.32268244431499227 1104 2 0.19812672439571344 1104 3 0.2184099217588198 1104 4 0.11515114408276617 1104 5 0.17736217298070045 1104 6 0.17193417730021565 1104 7 0.2591796052641584 1104 8 0.24938420344455636 1104 9 0.3685860123223961 1104 10-11 0.33129108804916285 1104 12-13 0.31361309575994767 1104 14-15 0.39546102039981434 1104 16-17 0.4129136518434464 1104 18-19 0.4172937341281795 1104 20-21 0.3495227656438331 1104 22-23 0.4287902148674263 1104 24-25 0.3553475178463188 1104 26-27 0.39775524194086387 1104 28-29 0.4346697227924565 1104 30-31 0.4741917494017329 1104 32-33 0.5545692907482973 1104 34-35 0.4064480882455186 1104 36-37 0.47437367262485 1104 38-39 0.3893437771457613 1104 40-41 0.5818931341673448 1104 42-43 0.6396789365080764 1104 44-45 0.7669898380872198 1104 46-47 0.8779786024918081 1104 48-49 0.9914627405675489 1104 50-51 0.9279448290004879 1104 52-53 0.98897834017572 1104 54-55 1.0365462852546514 1104 56-57 0.9616842197271964 1104 58-59 0.7744599275517814 1104 60-61 0.8400385354916509 1104 62-63 0.8185271586738949 1104 64-65 0.8630039826730567 1104 66-67 1.0288427195226575 1104 68-69 1.0185562045051242 1104 70-71 0.9783388517567104 1104 72-73 0.7978395439185348 1104 74-75 0.8231185818012499 1104 76-77 0.7801883764768185 1104 78-79 0.866761714293208 1104 80-81 0.9418745380960409 1104 82-83 0.9315514951141886 1104 84-85 0.9361636131194224 1104 86-87 0.7874160862381672 1104 88-89 0.8934409118247721 1104 90-91 1.029098295705932 1104 92-93 1.1774894585139286 1104 94-95 1.0904667654169096 1104 96-97 1.228422932533519 1104 98-99 1.0547135714892022 1104 100-101 0.8849287191796229 1105 1 0.152884506824023 1105 2 -0.011963051008066827 1105 3 0.06834144774997242 1105 4 0.07679909745442615 1105 5 0.015954227061598658 1105 6 0.06750636254899689 1105 7 0.16142571412866147 1105 8 0.07116840065556573 1105 9 0.1906170387136754 1105 10-11 0.19030138005578578 1105 12-13 0.2759600928427375 1105 14-15 0.25160160227117956 1105 16-17 0.2324136705481834 1105 18-19 0.19597964931430312 1105 20-21 0.18194922485174203 1105 22-23 0.19703356863415422 1105 24-25 0.22142691688878813 1105 26-27 0.22979495319840026 1105 28-29 0.32251941885882474 1105 30-31 0.2897989821935596 1105 32-33 0.2731070057335785 1105 34-35 0.3110542948441051 1105 36-37 0.17966898375064488 1105 38-39 0.17793733494083597 1105 40-41 -0.11144489857841933 1105 42-43 0.006866499544877058 1105 44-45 0.11710144301969905 1105 46-47 0.1357498706950011 1105 48-49 0.11177381135141218 1105 50-51 0.15632765409065996 1105 52-53 0.145750445324218 1105 54-55 0.16330682280679554 1105 56-57 0.14291698661261165 1105 58-59 0.1301468278540625 1105 60-61 0.12899756918292127 1105 62-63 0.09009500442362395 1105 64-65 0.19793999413355579 1105 66-67 0.16094089225320118 1105 68-69 0.13809194559048876 1105 70-71 -0.009800694385667441 1105 72-73 0.0793260350638576 1105 74-75 0.10421080642533909 1105 76-77 0.026334100317139786 1105 78-79 0.18418404012092893 1105 80-81 0.3071252439248049 1105 82-83 0.4454509487158056 1105 84-85 0.34820825362537633 1105 86-87 0.24093554760041513 1105 88-89 0.45379999876952937 1105 90-91 0.6370943141642051 1105 92-93 0.5717761515237108 1105 94-95 0.5618884941600761 1105 96-97 0.8524452432507488 1105 98-99 0.8139073166250554 1105 100-101 0.7346521091235125 1106 1 0.35886375137089743 1106 2 0.24107678291701262 1106 3 0.26211148718591915 1106 4 0.23961587821774089 1106 5 0.17157514522589423 1106 6 0.2535696223852497 1106 7 0.22423477002249115 1106 8 0.2757053436215884 1106 9 0.3961323021757295 1106 10-11 0.35529236933777497 1106 12-13 0.3495311631087361 1106 14-15 0.5342157078828578 1106 16-17 0.480309621445393 1106 18-19 0.4280733529589753 1106 20-21 0.32649424470661614 1106 22-23 0.43936296880293213 1106 24-25 0.5083997067694028 1106 26-27 0.496308084009776 1106 28-29 0.5440559638473772 1106 30-31 0.4555257897396743 1106 32-33 0.3793738305482037 1106 34-35 0.33359673517927035 1106 36-37 0.33641318594274594 1106 38-39 0.3834697104555431 1106 40-41 0.4841283600746564 1106 42-43 0.4404404446357191 1106 44-45 0.4856222087400468 1106 46-47 0.20956368765079247 1106 48-49 0.13475383131872576 1106 50-51 0.1808382249389524 1106 52-53 0.25499574513226975 1106 54-55 0.4856900721606934 1106 56-57 0.05571787761275715 1106 58-59 0.2012686744694463 1106 60-61 0.1808556447516949 1106 62-63 0.2044245395331572 1106 64-65 0.2774223007600298 1106 66-67 0.08901233458411895 1106 68-69 0.16495640151033086 1106 70-71 -0.02285987959772484 1106 72-73 0.17877688484839638 1106 74-75 0.12107644552139263 1106 76-77 0.14643406403958892 1106 78-79 0.20043266748984934 1106 80-81 0.044791120829497544 1106 82-83 0.3560042938287502 1106 84-85 0.3680281663832403 1106 86-87 0.21848502343380005 1106 88-89 0.4397456254666743 1106 90-91 0.5935979208959914 1106 92-93 0.7404589439369076 1106 94-95 0.6366950546277153 1106 96-97 0.8645532427931215 1106 98-99 1.059372867129568 1106 100-101 1.4448362739233396 1107 1 0.19169384927938893 1107 2 0.12700306927023775 1107 3 0.11794250113298688 1107 4 0.13479920675486312 1107 5 0.12125068743506517 1107 6 0.13641004494923692 1107 7 0.0743004524053319 1107 8 0.07969725084906543 1107 9 0.16387258656506987 1107 10-11 0.01598382469682491 1107 12-13 0.17340297482216727 1107 14-15 0.24986645773842753 1107 16-17 0.1992508060568241 1107 18-19 0.2664916706552134 1107 20-21 0.12925872613431721 1107 22-23 0.23283031762910156 1107 24-25 0.23320617439721048 1107 26-27 0.23374770125062838 1107 28-29 0.18371724924502786 1107 30-31 0.20951780680391607 1107 32-33 0.2280863240403761 1107 34-35 0.22227031350715976 1107 36-37 0.04593092567795054 1107 38-39 0.18125776285206996 1107 40-41 0.22195432812095106 1107 42-43 0.30849601544638006 1107 44-45 0.11999705084971168 1107 46-47 0.05104288127323997 1107 48-49 0.08898210725502764 1107 50-51 0.0838682916538005 1107 52-53 0.22121215430495766 1107 54-55 0.13138084653181892 1107 56-57 0.14834102109690406 1107 58-59 0.13309793981528628 1107 60-61 0.14141298942604408 1107 62-63 0.13117660061996617 1107 64-65 0.10001149370147999 1107 66-67 -0.0445247276340055 1107 68-69 -0.06170328686081206 1107 70-71 0.033045933183316833 1107 72-73 0.0425323464813161 1107 74-75 0.051530554624442004 1107 76-77 0.08604032122016747 1107 78-79 0.2697393888405806 1107 80-81 0.20097103124663818 1107 82-83 0.3749308152326378 1107 84-85 0.47946058052933793 1107 86-87 0.29684998243173766 1107 88-89 0.311064721026856 1107 90-91 0.5753878097631606 1107 92-93 0.6681501093269056 1107 94-95 0.47579133972879717 1107 96-97 0.5556497102232285 1107 98-99 0.6330705875774356 1107 100-101 0.784742879725794 1108 1 0.01848730395343523 1108 2 -0.1288960825592511 1108 3 -0.019494966607418007 1108 4 0.03454800611762465 1108 5 0.10106935504473569 1108 6 0.10230551762257534 1108 7 0.14701156184951714 1108 8 0.05834742918097646 1108 9 0.0941481124968675 1108 10-11 0.09749343068776994 1108 12-13 0.05397729824143482 1108 14-15 -0.04085340666859594 1108 16-17 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-0.9603922473468174 2316 74-75 -1.2548380853355283 2316 76-77 -1.4555891065099615 2316 78-79 -1.1813235050486135 2316 80-81 -1.110280847159391 2316 82-83 -1.0245508023115484 2316 84-85 -1.1389108887668549 2316 86-87 -0.9813756912844056 2316 88-89 -0.9270451539984528 2316 90-91 -0.8849407899046966 2316 92-93 -1.2834516175721937 2316 94-95 -1.3923906430450081 2316 96-97 -1.1264299671106457 2316 98-99 -1.0768948810241739 2316 100-101 -1.0483170251729597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 2.0 4 6.0 5 42.0 6 74.0 7 139.0 8 368.0 9 729.0 10 1208.0 11 2111.0 12 3288.0 13 4638.0 14 6086.0 15 8478.0 16 11664.0 17 16508.0 18 14781.0 19 8534.0 20 6858.0 21 7141.0 22 7390.0 23 7945.0 24 9023.0 25 10830.0 26 13483.0 27 16827.0 28 20692.0 29 26093.0 30 33196.0 31 42013.0 32 54359.0 33 71250.0 34 98887.0 35 142024.0 36 220909.0 37 341380.0 38 263718.0 39 22555.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10270093821782 19.43019287446771 15.091219709932426 22.375886477382036 2 15.575353698028763 20.83464307832654 40.13344016393772 23.456563059706983 3 19.252374452158826 25.048238113359734 30.37713677285851 25.322250661622924 4 13.032015746040917 17.513591527206795 37.18096109435787 32.27343163239442 5 13.688314902069628 37.43371265955277 33.96373048331488 14.914241955062717 6 31.316448940528762 36.53545582042057 17.542888136717703 14.605207102332956 7 28.094350512595696 33.2992918160581 20.729812678024093 17.87654499332211 8 26.52588156770111 34.484903366295846 19.810410945711507 19.17880412029154 9 26.641047488219233 16.70012854175333 20.476193023968158 36.18263094605928 10-11 24.571379654080783 27.203677552703308 27.204981696838654 21.019961096377255 12-13 25.90801824881347 24.405698022465582 28.03977741075647 21.646506317964473 14-15 22.891734671629994 25.474975823182188 26.796742182160116 24.8365473230277 16-17 21.84581142483384 28.790142546250287 27.285361354811354 22.078684674104522 18-19 21.59307506383616 26.780958699441026 30.430554682410914 21.1954115543119 20-21 22.365726022330815 26.004707736130015 30.629678935606208 20.99988730593296 22-23 22.088616229968018 25.93771275934068 30.540203071893764 21.43346793879754 24-25 22.05121276854734 26.092448103030147 30.615700915077444 21.24063821334507 26-27 21.82460779730459 26.41935007079278 30.36485200396734 21.391190127935293 28-29 21.50845358811418 26.32525489175816 30.672585187134644 21.49370633299302 30-31 21.766408846726794 26.202648747032555 30.592031551113703 21.438910855126956 32-33 21.669315646862238 26.28245363002803 30.478498451241098 21.569732271868634 34-35 22.02678630247568 26.25884954198573 30.287395469984208 21.426968685554385 36-37 21.985660493455594 26.101045366078772 30.16422881679006 21.749065323675566 38-39 21.81793800670682 26.31989561220818 30.409231176690117 21.452935204394883 40-41 21.950874498928382 26.187615392358264 30.302497273722295 21.55901283499106 42-43 21.860371949992008 26.244631992621727 30.04917064662209 21.84582541076417 44-45 22.01520773956838 26.48185842202397 29.754575480420847 21.748358357986806 46-47 22.09638160193709 26.7657860795655 29.189822191651004 21.94801012684641 48-49 22.339836781236365 26.942465469416966 28.757233406844758 21.96046434250192 50-51 22.261699506565527 27.057637801173595 28.609602242859644 22.07106044940123 52-53 22.512083530350445 27.145491256453074 28.387785853157098 21.95463936003938 54-55 22.214809560928565 27.047641787211585 28.49571910450147 22.24182954735838 56-57 22.2773568463426 27.465224390377664 28.33983958310065 21.91757918017909 58-59 22.447945682800558 27.043900651209825 28.46135377005478 22.04679989593484 60-61 22.39969930775911 26.859922960989262 28.888826467605195 21.851551263646435 62-63 22.232540191033646 26.92896228951455 28.97579910569182 21.862698413759986 64-65 22.159998528656917 26.96272987645734 28.69761057226886 22.179661022616884 66-67 22.139812230058975 27.386984244606534 28.31643742718531 22.156766098149188 68-69 22.151382325442036 27.08783374374847 28.62167393197319 22.139109998836304 70-71 22.156164185672612 27.150967674624845 28.515001665291184 22.177866474411363 72-73 22.25357368813176 27.005706130277936 28.35820346514325 22.382516716447057 74-75 22.20179424381331 27.318880222789065 28.147584378548796 22.33174115484883 76-77 22.251359410336434 27.30451025614531 28.242261785931287 22.20186854758697 78-79 22.256598238998254 27.214569545724952 28.12409608297327 22.404736132303523 80-81 22.172085521402924 27.465455448991378 28.051890983720863 22.310568045884835 82-83 22.285706642233006 27.303647325951957 28.1063800615969 22.304265970218133 84-85 22.176014435734594 27.15110603850978 28.059124713203136 22.613754812552482 86-87 22.241026747123726 27.108712335913243 28.141746212482214 22.508514704480817 88-89 22.22239685342805 27.09400557578863 28.05727873826799 22.626318832515334 90-91 22.04068939226741 27.127833176166877 28.26698902509982 22.564488406465895 92-93 22.00632877741169 26.91178334353798 28.33012368971134 22.75176418933899 94-95 21.859687760307033 26.959607204111474 28.279203961666084 22.90150107391541 96-97 22.050525445640638 26.941654232490887 28.15305840595984 22.854761915908632 98-99 21.89187074302163 26.823779153446516 28.037770400055646 23.246579703476208 100-101 21.594462082143153 27.032193946171095 28.144405602122497 23.228938369563252 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 448.0 1 331.0 2 249.5 3 280.0 4 230.5 5 241.0 6 428.0 7 952.0 8 1679.0 9 2092.5 10 1977.5 11 1773.5 12 1843.5 13 2032.0 14 2209.0 15 2322.0 16 2497.0 17 2647.5 18 2737.5 19 2925.0 20 3222.5 21 3685.5 22 4258.5 23 4933.5 24 5740.0 25 6985.0 26 8751.0 27 10859.0 28 12828.5 29 15009.5 30 17594.5 31 20403.5 32 23578.0 33 26691.0 34 30921.5 35 34426.5 36 37831.0 37 41683.5 38 43545.0 39 44313.5 40 45185.0 41 46708.5 42 48641.5 43 51077.5 44 53127.5 45 54675.0 46 54919.5 47 55123.5 48 57852.0 49 57917.0 50 55767.0 51 54131.5 52 51707.0 53 50628.5 54 48693.5 55 44163.5 56 39490.5 57 35645.0 58 32554.0 59 29129.0 60 24841.0 61 19680.0 62 14918.0 63 11478.5 64 8535.5 65 6323.0 66 4583.5 67 3483.0 68 2503.5 69 1518.0 70 1018.0 71 694.5 72 487.0 73 358.5 74 263.0 75 191.5 76 118.5 77 62.5 78 37.0 79 23.5 80 13.5 81 8.5 82 9.5 83 6.0 84 3.0 85 2.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01819113262539509 2 0.002140133250046481 3 0.003143320711005769 4 6.687916406395254E-5 5 0.0026082873984941486 6 4.681541484476677E-4 7 6.687916406395254E-5 8 0.0 9 0.0 10-11 3.343958203197627E-5 12-13 9.028687148633593E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002374210324270315 22-23 0.0 24-25 0.004012749843837152 26-27 0.0016719791015988134 28-29 0.0027086061445900774 30-31 0.017589220148819515 32-33 3.009562382877864E-4 34-35 0.0019729353398865996 36-37 0.004280266500092962 38-39 0.0026751665625581013 40-41 0.004246826918060985 42-43 0.0022070124141104334 44-45 5.684728945435965E-4 46-47 3.343958203197627E-5 48-49 1.003187460959288E-4 50-51 0.0 52-53 0.001136945789087193 54-55 0.0029761228008458878 56-57 3.3439582031976266E-4 58-59 0.001136945789087193 60-61 0.0011703853711191694 62-63 0.0 64-65 1.003187460959288E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.350333125116203E-4 76-77 5.684728945435965E-4 78-79 3.6783540235173895E-4 80-81 0.006654476824363277 82-83 0.0019394957578546233 84-85 0.003711793605549365 86-87 0.001772297847694742 88-89 0.0013041436992470743 90-91 2.6751665625581014E-4 92-93 3.343958203197627E-5 94-95 3.009562382877864E-4 96-97 0.0018057374297267186 98-99 0.0028089248906860063 100-101 0.001003187460959288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1495234.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.705230465147494 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58829224708306 47.30954269157671 2 11.479252538196286 13.477843316448848 3 3.3103771247454663 5.830093561042047 4 1.4196099629132626 3.3335412017377033 5 0.7494106470280396 2.199716237340819 6 0.4858285682459244 1.711240683925778 7 0.32415979415558904 1.332091279640704 8 0.23862563320810232 1.1206858233898722 9 0.17925608017020986 0.9470942548804014 >10 1.0806991014803053 11.936929446058677 >50 0.09326221019009091 3.787262366966703 >100 0.04951097540206499 5.127768191250043 >500 0.0011434114543574599 0.41100167831944384 >1k 3.430234363072379E-4 0.48013963825232436 >5k 2.2868229087149195E-4 0.9950496291699226 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8308 0.5556320950433177 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6516 0.43578463304071474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4364 0.29186067197508886 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1740 0.11636974547127742 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.687916406395254E-5 0.0 10-11 0.0 0.0 0.0 6.687916406395254E-5 6.687916406395254E-5 12-13 0.0 6.687916406395254E-5 0.0 6.687916406395254E-5 6.687916406395254E-5 14-15 0.0 6.687916406395254E-5 0.0 6.687916406395254E-5 6.687916406395254E-5 16-17 0.0 6.687916406395254E-5 0.0 2.006374921918576E-4 6.687916406395254E-5 18-19 0.0 6.687916406395254E-5 0.0 4.681541484476677E-4 6.687916406395254E-5 20-21 0.0 6.687916406395254E-5 0.0 5.350333125116203E-4 6.687916406395254E-5 22-23 0.0 6.687916406395254E-5 0.0 8.69429132831383E-4 6.687916406395254E-5 24-25 0.0 6.687916406395254E-5 0.0 9.363082968953354E-4 6.687916406395254E-5 26-27 0.0 6.687916406395254E-5 0.0 0.0012372645351831218 1.3375832812790507E-4 28-29 0.0 6.687916406395254E-5 0.0 0.002374210324270315 1.3375832812790507E-4 30-31 0.0 6.687916406395254E-5 0.0 0.004882178976668535 1.3375832812790507E-4 32-33 0.0 6.687916406395254E-5 0.0 0.007423587211098731 1.3375832812790507E-4 34-35 0.0 6.687916406395254E-5 0.0 0.009864676699432997 1.3375832812790507E-4 36-37 0.0 6.687916406395254E-5 0.0 0.012640162008087028 1.3375832812790507E-4 38-39 0.0 6.687916406395254E-5 0.0 0.017689538894915445 1.3375832812790507E-4 40-41 0.0 6.687916406395254E-5 0.0 0.022772355363775837 1.3375832812790507E-4 42-43 0.0 6.687916406395254E-5 0.0 0.031466646692089664 1.3375832812790507E-4 44-45 0.0 6.687916406395254E-5 0.0 0.03868959641099654 1.3375832812790507E-4 46-47 0.0 6.687916406395254E-5 0.0 0.050627527196412066 1.3375832812790507E-4 48-49 0.0 6.687916406395254E-5 0.0 0.06437119541155431 1.3375832812790507E-4 50-51 0.0 6.687916406395254E-5 0.0 0.07012280352105424 1.3375832812790507E-4 52-53 0.0 6.687916406395254E-5 0.0 0.08132506350176628 1.3375832812790507E-4 54-55 0.0 6.687916406395254E-5 0.0 0.10426461677570201 1.3375832812790507E-4 56-57 0.0 6.687916406395254E-5 0.0 0.13994465080382068 1.3375832812790507E-4 58-59 0.0 6.687916406395254E-5 0.0 0.17572500357803528 1.3375832812790507E-4 60-61 0.0 6.687916406395254E-5 0.0 0.22414551836033692 1.3375832812790507E-4 62-63 0.0 6.687916406395254E-5 0.0 0.27152940609964726 1.3375832812790507E-4 64-65 0.0 6.687916406395254E-5 0.0 0.31650564393265535 1.3375832812790507E-4 66-67 0.0 6.687916406395254E-5 0.0 0.38886889944985203 1.3375832812790507E-4 68-69 0.0 6.687916406395254E-5 0.0 0.48233253122922565 1.3375832812790507E-4 70-71 0.0 6.687916406395254E-5 0.0 0.5335285313201814 1.3375832812790507E-4 72-73 0.0 6.687916406395254E-5 0.0 0.5992707495950467 1.6719791015988133E-4 74-75 0.0 6.687916406395254E-5 0.0 0.6775528111319031 2.006374921918576E-4 76-77 0.0 6.687916406395254E-5 0.0 0.7471405813404457 2.006374921918576E-4 78-79 0.0 6.687916406395254E-5 0.0 0.822814355478808 2.006374921918576E-4 80-81 0.0 6.687916406395254E-5 0.0 0.9640631499818757 2.006374921918576E-4 82-83 0.0 6.687916406395254E-5 0.0 1.1274489477901117 2.006374921918576E-4 84-85 0.0 6.687916406395254E-5 0.0 1.3558747326505416 2.006374921918576E-4 86-87 0.0 6.687916406395254E-5 0.0 1.6733166848800924 2.006374921918576E-4 88-89 0.0 6.687916406395254E-5 0.0 2.0085819343326863 2.006374921918576E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4060 0.0 49.491493 1 GTATCAA 7370 0.0 41.70163 1 ATCAACG 9535 0.0 31.880999 3 TATCAAC 9645 0.0 31.566648 2 TCAACGC 9700 0.0 31.338696 4 CAACGCA 9870 0.0 30.797888 5 AACGCAG 10175 0.0 29.969072 6 ACGCAGA 11415 0.0 26.339071 7 CGCAGAG 11495 0.0 25.99048 8 GCAGAGT 11945 0.0 24.653479 9 GTACATG 6885 0.0 23.940971 1 TACATGG 7080 0.0 23.010895 2 ACATGGG 7090 0.0 22.442501 3 GAGTACT 7205 0.0 22.314651 12-13 GTGGTAT 2265 0.0 21.391842 1 AGAGTAC 11075 0.0 21.164892 10-11 GTACTTT 7655 0.0 20.971857 14-15 AGTACTT 7580 0.0 20.803398 12-13 TGGTATC 2285 0.0 20.578876 2 CAGAGTA 11775 0.0 20.189049 10-11 >>END_MODULE