FastQCFastQC Report
Thu 26 May 2016
SRR936814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170009
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49510.4231591380921001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37300.318800966488292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23670.20230613610664533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29850.039.7941931
GTATCAA52300.039.610361
ATCAACG65300.031.4363233
TCAACGC66200.031.0820484
CAACGCA67050.030.5514985
TATCAAC66950.030.5170042
AACGCAG69050.029.7316066
GTACATG51800.026.5089241
ACGCAGA78750.025.8302547
CGCAGAG79250.025.6095098
TACATGG55100.024.748812
TGGACCG1351.3365288E-524.6444035
GCAGAGT82700.023.6790529
ACATGGG55350.023.0938243
GGACCGT1302.9321905E-421.9334136
GTGTACG1353.7883106E-421.117451
GTACACG1604.977855E-520.787491
GAGTACT44000.020.57591812-13
AGAGTAC74750.020.41022910-11
AGTACTT47000.020.27371812-13
Produced by FastQC (version 0.11.5)