##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936814_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1170009 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90703917662172 33.0 31.0 34.0 30.0 34.0 2 32.02680748609626 33.0 31.0 34.0 30.0 34.0 3 32.03688176757615 34.0 31.0 34.0 30.0 34.0 4 35.614047413310495 37.0 35.0 37.0 33.0 37.0 5 35.623335376052665 37.0 35.0 37.0 33.0 37.0 6 35.68960324236822 37.0 35.0 37.0 33.0 37.0 7 35.63242932319324 37.0 35.0 37.0 33.0 37.0 8 35.578802385280795 37.0 35.0 37.0 33.0 37.0 9 37.24207591565535 39.0 37.0 39.0 34.0 39.0 10-11 37.07116398249928 39.0 37.0 39.0 33.0 39.0 12-13 37.017540890711096 39.0 37.0 39.0 33.0 39.0 14-15 38.22136752794209 40.0 38.0 41.0 33.0 41.0 16-17 38.156629137040824 40.0 38.0 41.0 33.0 41.0 18-19 38.13197077971195 40.0 38.0 41.0 33.0 41.0 20-21 38.06327344490512 40.0 38.0 41.0 33.0 41.0 22-23 37.95772340212768 40.0 38.0 41.0 33.0 41.0 24-25 37.80528611318375 40.0 37.5 41.0 33.0 41.0 26-27 37.8973815586034 40.0 38.0 41.0 33.0 41.0 28-29 37.84631229332424 40.0 38.0 41.0 33.0 41.0 30-31 37.704772356451954 40.0 37.0 41.0 33.0 41.0 32-33 37.51719132075053 40.0 37.0 41.0 32.0 41.0 34-35 37.361398929409944 40.0 37.0 41.0 32.0 41.0 36-37 37.19665532487357 40.0 36.5 41.0 31.0 41.0 38-39 37.04415051508151 40.0 36.0 41.0 31.0 41.0 40-41 36.86940228664908 39.0 36.0 41.0 31.0 41.0 42-43 36.65841843951628 39.0 36.0 41.0 30.0 41.0 44-45 36.40013922969823 39.0 35.5 41.0 30.0 41.0 46-47 36.03975524974594 39.0 35.0 40.5 29.5 41.0 48-49 35.71748123305034 39.0 35.0 40.0 28.0 41.0 50-51 35.078724180754165 38.5 34.0 40.0 27.0 40.5 52-53 35.30769036819375 38.5 35.0 40.0 28.0 40.5 54-55 35.69288655044534 39.0 35.0 40.5 28.0 41.0 56-57 35.59880394082439 39.0 35.0 41.0 28.0 41.0 58-59 35.35629896864042 39.0 35.0 41.0 27.5 41.0 60-61 35.090424518102 38.0 35.0 40.0 26.5 41.0 62-63 34.7734282385862 38.0 34.0 40.0 26.0 41.0 64-65 34.45419052332076 37.5 34.0 40.0 26.0 41.0 66-67 34.0883403460999 37.0 34.0 40.0 25.5 41.0 68-69 33.71735858442115 36.5 34.0 39.0 25.0 41.0 70-71 33.299586157029566 36.0 33.0 39.0 23.5 41.0 72-73 32.90475030533953 35.5 33.0 38.5 23.0 40.0 74-75 32.472794226369196 35.0 32.5 37.5 22.0 39.5 76-77 32.045635973740374 35.0 32.0 37.0 20.5 39.0 78-79 31.61097863349769 35.0 32.0 36.5 19.5 39.0 80-81 31.191262631313094 35.0 31.0 36.0 18.0 38.5 82-83 30.79080032717697 35.0 31.0 35.5 16.0 37.0 84-85 30.441086777964955 34.5 31.0 35.0 12.0 37.0 86-87 30.124950748242107 34.0 31.0 35.0 7.5 36.0 88-89 29.794930209938556 34.0 30.5 35.0 4.5 36.0 90-91 29.465305822433848 34.0 30.0 35.0 2.0 36.0 92-93 29.116888844444787 34.0 29.5 35.0 2.0 35.0 94-95 28.72440297467797 34.0 29.0 35.0 2.0 35.0 96-97 28.280924334770074 34.0 29.0 35.0 2.0 35.0 98-99 27.703091172802942 34.0 29.0 35.0 2.0 35.0 100-101 25.52482459536636 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3430099275015692 1101 2 0.3963099403933299 1101 3 0.3709633935311203 1101 4 0.2864770360367075 1101 5 0.26739063000830754 1101 6 0.14096042016398513 1101 7 0.08918356687075857 1101 8 0.16488321077709145 1101 9 0.10034429514744403 1101 10-11 0.20740127251003315 1101 12-13 0.16233226674922463 1101 14-15 0.4404031663511532 1101 16-17 0.5635987361778305 1101 18-19 0.4117637137195871 1101 20-21 0.45694538136355334 1101 22-23 0.5825829349713842 1101 24-25 0.5541184832976711 1101 26-27 0.5620310223803457 1101 28-29 0.6111822252509285 1101 30-31 0.4380005078221103 1101 32-33 0.5102963232445106 1101 34-35 0.4498983601792048 1101 36-37 0.4659595575617814 1101 38-39 0.31801820908656 1101 40-41 0.2867428650486801 1101 42-43 0.32515471295777587 1101 44-45 0.5246248257025101 1101 46-47 0.26496944083213947 1101 48-49 0.2821715014264825 1101 50-51 0.37190744730811787 1101 52-53 0.4665239101178713 1101 54-55 0.42599881995440114 1101 56-57 0.44896466477759844 1101 58-59 0.32572706414521235 1101 60-61 0.40608613100486224 1101 62-63 0.38160529940267196 1101 64-65 0.45794888699931136 1101 66-67 0.45030966456069166 1101 68-69 0.3645414060037879 1101 70-71 0.4600417514202846 1101 72-73 0.3889438832946439 1101 74-75 0.32500942418466394 1101 76-77 0.38435330256067957 1101 78-79 0.32774858339336177 1101 80-81 0.17141228343492543 1101 82-83 0.2599206029722332 1101 84-85 0.3905680559243301 1101 86-87 0.32753899252223917 1101 88-89 0.4925751446826183 1101 90-91 0.5432847970861125 1101 92-93 0.5688495355185879 1101 94-95 0.5141561762217499 1101 96-97 0.5541535241309887 1101 98-99 0.5798859421534885 1101 100-101 0.6372353122449397 1102 1 0.36933734158408527 1102 2 0.366819702739825 1102 3 0.34188131914292796 1102 4 0.23807700069789206 1102 5 0.22364118217722506 1102 6 0.16309135045818124 1102 7 0.16692718379805171 1102 8 0.21970254110662069 1102 9 0.32265191949588257 1102 10-11 0.4699598024155094 1102 12-13 0.5019223365433803 1102 14-15 0.4864849756982679 1102 16-17 0.5553135519742725 1102 18-19 0.47594694545384186 1102 20-21 0.35331166813799797 1102 22-23 0.35287713057169867 1102 24-25 0.38703923601433843 1102 26-27 0.33842999755483305 1102 28-29 0.20527180913642695 1102 30-31 0.3588203682338076 1102 32-33 0.48587720494784037 1102 34-35 0.3106740470773488 1102 36-37 0.2605562533829655 1102 38-39 0.30732821880472727 1102 40-41 0.14721198776270228 1102 42-43 0.2544417524488978 1102 44-45 0.3850904145354406 1102 46-47 0.41250720918623074 1102 48-49 0.5570120350425327 1102 50-51 0.5669776831946791 1102 52-53 0.4921436645131365 1102 54-55 0.44990022467213464 1102 56-57 0.5300407315679507 1102 58-59 0.4855044296368618 1102 60-61 0.3662151176644599 1102 62-63 0.31607212509399574 1102 64-65 0.5201672808503517 1102 66-67 0.26670245544327287 1102 68-69 0.2581645175860885 1102 70-71 0.5083958463049854 1102 72-73 0.328680609153821 1102 74-75 0.2974460351927064 1102 76-77 0.4504041904482996 1102 78-79 0.39868241147004824 1102 80-81 0.43752589771180794 1102 82-83 0.2552125898968747 1102 84-85 0.31516386827524556 1102 86-87 0.2838840760101853 1102 88-89 0.31293648402355245 1102 90-91 0.401121178391886 1102 92-93 0.48305132012853846 1102 94-95 0.47301914998268657 1102 96-97 0.36514742818611623 1102 98-99 0.5075270765293176 1102 100-101 0.5387071737168014 1103 1 0.2830483758191349 1103 2 0.32855432910326954 1103 3 0.36790789809526814 1103 4 0.25165457305276107 1103 5 0.2929014706514863 1103 6 0.23092439078030935 1103 7 0.15764194211106286 1103 8 0.2795203070379273 1103 9 0.2880255560485594 1103 10-11 0.35272318168543393 1103 12-13 0.3083493011362819 1103 14-15 0.3408054732502066 1103 16-17 0.2673455374560447 1103 18-19 0.26292798479295953 1103 20-21 0.44648412886504474 1103 22-23 0.530304681187026 1103 24-25 0.4460303322543453 1103 26-27 0.35660879110480437 1103 28-29 0.3270491584246997 1103 30-31 0.2344255781621598 1103 32-33 0.49741995622858326 1103 34-35 0.4048947317783771 1103 36-37 0.3410636353750718 1103 38-39 0.3997590776111366 1103 40-41 0.42842618840921887 1103 42-43 0.3739166007447068 1103 44-45 0.5013419504812973 1103 46-47 0.5406782521171039 1103 48-49 0.5297124641621735 1103 50-51 0.5517335386928934 1103 52-53 0.6053312483994091 1103 54-55 0.6075716362300128 1103 56-57 0.6773043849451383 1103 58-59 0.7208790113869838 1103 60-61 0.66209972795955 1103 62-63 0.7648815543837628 1103 64-65 0.7288923985274423 1103 66-67 0.6618734416623013 1103 68-69 0.7404284545225082 1103 70-71 0.7422562217374136 1103 72-73 0.5919751893916327 1103 74-75 0.5902888110485875 1103 76-77 0.8189682464796029 1103 78-79 0.6953628780195125 1103 80-81 0.5910081941635354 1103 82-83 0.6342951812502058 1103 84-85 0.5748360737989806 1103 86-87 0.5766879097310458 1103 88-89 0.572034576615092 1103 90-91 0.6575768769873207 1103 92-93 0.6315635793394847 1103 94-95 0.6115781019477495 1103 96-97 0.8370478421825993 1103 98-99 0.7026277400579275 1103 100-101 0.42500823800913423 1104 1 0.3448250958389636 1104 2 0.3191443187630725 1104 3 0.18749360639904467 1104 4 0.2687287234044149 1104 5 0.2474477409152911 1104 6 0.24914365431870777 1104 7 0.19229870725176568 1104 8 0.24581296036772926 1104 9 0.15557014282506998 1104 10-11 0.35509886426689974 1104 12-13 0.48704271844458447 1104 14-15 0.643157869014992 1104 16-17 0.6090308620614522 1104 18-19 0.5131345751757195 1104 20-21 0.3753975959088578 1104 22-23 0.43899792758291056 1104 24-25 0.5172260355162095 1104 26-27 0.3983573533040996 1104 28-29 0.4384287207180009 1104 30-31 0.48275768662318796 1104 32-33 0.5927057645161327 1104 34-35 0.6058400910790027 1104 36-37 0.6832046552094582 1104 38-39 0.6431729676631761 1104 40-41 0.6942231957328104 1104 42-43 0.706372013968199 1104 44-45 0.6761095095956264 1104 46-47 0.7454648679658078 1104 48-49 0.6288696523111028 1104 50-51 0.47784318755334 1104 52-53 0.47378957567959645 1104 54-55 0.6359463018825977 1104 56-57 0.569330893460851 1104 58-59 0.4938917272707357 1104 60-61 0.5528381977806944 1104 62-63 0.6645548980288183 1104 64-65 0.75587711895745 1104 66-67 0.5330785451070383 1104 68-69 0.40173238807176403 1104 70-71 0.4330658338516855 1104 72-73 0.446891485035934 1104 74-75 0.4742476126107178 1104 76-77 0.33301938347445414 1104 78-79 0.40841674106739134 1104 80-81 0.4791552044780971 1104 82-83 0.5957646862023971 1104 84-85 0.4671875295454804 1104 86-87 0.29323650440370486 1104 88-89 0.4536608509807962 1104 90-91 0.4405151177858251 1104 92-93 0.48397493988777285 1104 94-95 0.555690382859396 1104 96-97 0.4055832936904764 1104 98-99 0.5214560927186085 1104 100-101 0.4123331596030617 1105 1 0.05248284621719179 1105 2 0.07896595868773204 1105 3 0.10257753752509302 1105 4 0.04265501221805579 1105 5 0.1949910444361791 1105 6 0.2327382325017595 1105 7 0.15559234783511755 1105 8 0.18241228315520885 1105 9 0.29412700088832366 1105 10-11 0.2501000478450024 1105 12-13 0.12370044823884285 1105 14-15 0.24729138691790098 1105 16-17 0.12880716579847018 1105 18-19 0.14939297600731294 1105 20-21 0.22098259225520422 1105 22-23 0.23984933192407 1105 24-25 0.25430445013905967 1105 26-27 0.23724792893474245 1105 28-29 0.24065873625889367 1105 30-31 0.027360313137393177 1105 32-33 -0.011693543862577371 1105 34-35 0.1361836889506165 1105 36-37 0.1190135536584549 1105 38-39 -0.050047188157449796 1105 40-41 0.19758764516315352 1105 42-43 0.3065829996314591 1105 44-45 0.2893303343951956 1105 46-47 0.37703012428765703 1105 48-49 0.6102702368957722 1105 50-51 0.34902125945313145 1105 52-53 0.21893304593061913 1105 54-55 0.30267257628745625 1105 56-57 0.6336470426122744 1105 58-59 0.4727471043175484 1105 60-61 0.5077127385009277 1105 62-63 0.43477735925377203 1105 64-65 0.5574700607151257 1105 66-67 0.5449518919571759 1105 68-69 0.48116977678969164 1105 70-71 0.41795257057245294 1105 72-73 0.6534088836341567 1105 74-75 0.6910423287063523 1105 76-77 0.6891194421387645 1105 78-79 0.7800247605809894 1105 80-81 0.5949063134393278 1105 82-83 0.6857485277881814 1105 84-85 0.6649760395750199 1105 86-87 0.5427646169568412 1105 88-89 0.41303916371300886 1105 90-91 0.2400267631652362 1105 92-93 0.19042672709071695 1105 94-95 0.222808781838431 1105 96-97 0.36525261225824224 1105 98-99 0.35573894730317335 1105 100-101 0.30131173169326075 1106 1 0.13829896415395382 1106 2 0.06402009684743604 1106 3 0.04004919937266038 1106 4 0.11287285776179345 1106 5 -0.011895789442220916 1106 6 0.13524188034067208 1106 7 0.10205178428347494 1106 8 0.17950116172998776 1106 9 0.2179754962627598 1106 10-11 0.3214389075028592 1106 12-13 0.4205573784773904 1106 14-15 0.25096722884031664 1106 16-17 0.2900925873718663 1106 18-19 0.21096653067961313 1106 20-21 0.33721117069729445 1106 22-23 0.09200624887607489 1106 24-25 0.32010318237725954 1106 26-27 0.09888177944991128 1106 28-29 0.13282569854599302 1106 30-31 0.21663355161400233 1106 32-33 0.2695794133923144 1106 34-35 0.25931173241605876 1106 36-37 0.15704581193438827 1106 38-39 0.2820837038694961 1106 40-41 0.22828400852543496 1106 42-43 0.16204754871448301 1106 44-45 0.1031311710298084 1106 46-47 0.05110428221630059 1106 48-49 0.015283728984329059 1106 50-51 0.016000825001320607 1106 52-53 -0.12569523198554577 1106 54-55 0.10682041788098928 1106 56-57 0.14559836980600238 1106 58-59 0.312108135209634 1106 60-61 0.169425652833759 1106 62-63 0.23490974795222996 1106 64-65 0.25476824689462063 1106 66-67 0.43910170567757945 1106 68-69 0.3877961230939775 1106 70-71 0.49357499705114805 1106 72-73 0.6103771943555643 1106 74-75 0.41018316061024507 1106 76-77 0.5096594712499787 1106 78-79 0.3760500610026476 1106 80-81 0.28227921999089745 1106 82-83 0.4630161608963199 1106 84-85 0.5735879184518744 1106 86-87 0.47138822134937897 1106 88-89 0.7046556583200427 1106 90-91 0.7451542930435551 1106 92-93 0.6897066922478245 1106 94-95 0.7288240667154362 1106 96-97 0.702198830392156 1106 98-99 0.7289797475696176 1106 100-101 0.9345277105682683 1107 1 0.12789474662036326 1107 2 0.07713411446268736 1107 3 0.1723522253884795 1107 4 0.2190899470629546 1107 5 0.22354159475369784 1107 6 0.08837232926028804 1107 7 0.07284836746571699 1107 8 0.07178676361338177 1107 9 0.19415568959120577 1107 10-11 0.3690279486667478 1107 12-13 0.2692832698198018 1107 14-15 0.2432355803361972 1107 16-17 0.29032279937788985 1107 18-19 0.27164476875729804 1107 20-21 0.2529175864763218 1107 22-23 0.0905055272141837 1107 24-25 0.34936958172496446 1107 26-27 0.2842530164764412 1107 28-29 0.1583447924910999 1107 30-31 0.17816735927094385 1107 32-33 0.17258228390644348 1107 34-35 0.19188474561198632 1107 36-37 0.3131540624596525 1107 38-39 0.003728844782827423 1107 40-41 0.23704695717513147 1107 42-43 0.3335924139939266 1107 44-45 0.5336378608293657 1107 46-47 0.3562431602461231 1107 48-49 0.538229846964569 1107 50-51 0.34537432567582727 1107 52-53 0.37252636475498946 1107 54-55 0.5419986739536782 1107 56-57 0.6006908951758589 1107 58-59 0.5245469676934817 1107 60-61 0.5938435143069469 1107 62-63 0.44204594782837603 1107 64-65 0.5535077275710805 1107 66-67 0.36805399904833536 1107 68-69 0.4298290502365276 1107 70-71 0.4653712339846052 1107 72-73 0.563659980330371 1107 74-75 0.4954670908193961 1107 76-77 0.46855328704809907 1107 78-79 0.5202288618897377 1107 80-81 0.529823521840413 1107 82-83 0.5164005255918518 1107 84-85 0.5461213302186216 1107 86-87 0.5429010058721815 1107 88-89 0.49167883713209903 1107 90-91 0.5682146483022557 1107 92-93 0.6143857916038513 1107 94-95 0.6117984927056881 1107 96-97 0.5992859969725721 1107 98-99 0.3375498393689824 1107 100-101 0.46309044302964963 1108 1 0.003205434275706409 1108 2 0.11467679023886035 1108 3 0.03897966836403555 1108 4 0.019467375029442735 1108 5 0.17253678785032633 1108 6 -0.06514239363233543 1108 7 -0.024304312753137935 1108 8 -0.09114764877170245 1108 9 0.059852570507281655 1108 10-11 0.08827337290298942 1108 12-13 0.09604463368358296 1108 14-15 0.1326447834949036 1108 16-17 0.17227549880833948 1108 18-19 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2316 56-57 -1.95888205332799 2316 58-59 -1.7953294121365815 2316 60-61 -1.9309172801390773 2316 62-63 -1.6500705093036743 2316 64-65 -1.9968383458868146 2316 66-67 -1.7608990630978383 2316 68-69 -2.151860565459131 2316 70-71 -1.9397080110606169 2316 72-73 -2.2071149301316524 2316 74-75 -1.928307473044022 2316 76-77 -2.43192039771521 2316 78-79 -2.6370507591624524 2316 80-81 -2.668572671876696 2316 82-83 -2.525249073719152 2316 84-85 -2.665508628504636 2316 86-87 -3.095617652686176 2316 88-89 -2.752586531365239 2316 90-91 -2.402027760663092 2316 92-93 -2.550075247561793 2316 94-95 -2.9582485606809996 2316 96-97 -2.9763250143601496 2316 98-99 -3.2044673645255273 2316 100-101 -3.5889072253069934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2327.0 3 790.0 4 769.0 5 824.0 6 911.0 7 1041.0 8 1290.0 9 1663.0 10 2064.0 11 2599.0 12 3372.0 13 4439.0 14 4825.0 15 6234.0 16 8160.0 17 10741.0 18 9497.0 19 6062.0 20 5658.0 21 5942.0 22 6446.0 23 7046.0 24 7917.0 25 9311.0 26 10818.0 27 12914.0 28 15257.0 29 18866.0 30 23279.0 31 29455.0 32 37610.0 33 50635.0 34 70451.0 35 103318.0 36 161445.0 37 250169.0 38 233557.0 39 42280.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.973797931811696 19.02497251023064 14.502542055499406 22.49868750245826 2 15.23478347114787 20.499381394994902 40.76867763881847 23.497157495038763 3 19.100529959964472 25.251023543422264 29.90148848732721 25.746958009286054 4 12.621068662081727 17.007886404310973 37.71587567476513 32.65516925884217 5 13.682220067016631 37.53658290443032 34.027531896187504 14.753665132365542 6 31.095031603075636 36.48277867583541 17.97637680787554 14.445812913213421 7 28.169962086775357 33.09158271676948 20.966021779791983 17.772433416663176 8 26.151314523433406 34.673586456461514 20.15853480179301 19.01656421831207 9 26.603273814869265 17.050128419275463 20.589676681060915 35.75692108479435 10-11 24.47108060787133 27.179336069843448 27.497821027448637 20.851762294836583 12-13 25.717758341897294 24.57251825802393 27.970989018884335 21.738734381194437 14-15 22.75535295048387 25.719991618308008 26.583134838331702 24.94152059287642 16-17 21.852885968025383 29.206022071808203 26.865478272395706 22.075613687770712 18-19 21.743413457838436 27.485356113662213 29.632556031570935 21.138674396928415 20-21 22.65283800783164 26.59525432309496 29.58945884027061 21.162448828802788 22-23 22.301063740653156 26.669650475442996 29.44805522354627 21.58123056035758 24-25 22.109782983150357 26.88458964094384 29.523891343688803 21.481736032217004 26-27 21.862824210448935 27.09840303220435 29.384053573046614 21.654719184300102 28-29 21.62283457499327 27.045234034732285 29.670543381161817 21.66138800911263 30-31 21.827338270937986 26.930360797886593 29.63144053739511 21.61086039378031 32-33 21.651553136402992 27.05485745643089 29.42920175088446 21.864387656281654 34-35 22.13499127690547 27.100573305193766 29.23634243766257 21.528092980238196 36-37 22.119348906503287 26.885706483113015 29.123728192782615 21.87121641760108 38-39 21.87389548174839 27.116646957181768 29.33643214587783 21.67302541519202 40-41 22.093323913841566 26.926625834622957 29.308423820715102 21.671626430820375 42-43 21.90071165677732 27.016802484901014 29.096727541271132 21.98575831705053 44-45 22.01440521439017 27.307898423323902 28.859275226432977 21.81842113585295 46-47 22.188712240906042 27.36061246198075 28.391496679605783 22.059178617507424 48-49 22.29193750069445 27.58468611457978 28.089046382170213 22.034330002555564 50-51 22.2177680431647 27.637288316683584 28.02047117901597 22.124472461135746 52-53 22.440589189082925 27.7067679134183 27.933060215548227 21.91958268195055 54-55 22.180946792447507 27.71983689462129 27.942758405820577 22.156457907110624 56-57 22.235648694523725 28.121416154197643 27.664521898139384 21.97841325313925 58-59 22.32208835032411 27.65852964293087 27.87953153240941 22.139850474335613 60-61 22.36023676570659 27.566201387630322 28.217144654819265 21.856417191843825 62-63 22.1743285603037 27.702320921632673 28.256970842946494 21.86637967511713 64-65 22.13992490666758 27.706875292498573 28.04332077503841 22.109879025795436 66-67 21.98456485649659 28.094551366988146 27.720426945506834 22.20045683100843 68-69 21.89492627398847 27.890852588585556 28.052132914138422 22.162088223287547 70-71 22.026360354491885 27.830023756461646 27.961885368874846 22.181730520171623 72-73 22.081051207108352 27.767210729548058 27.841840210843245 22.30989785250034 74-75 21.99993246532757 28.090619562229996 27.665236612854073 22.244211359588366 76-77 22.18252528115223 27.962405600431907 27.684555510387103 22.170513608028756 78-79 22.06100258473935 27.98097912286447 27.643714671452525 22.314303620943658 80-81 21.96846277060293 28.147022122741866 27.582529057179023 22.301986049476184 82-83 22.140288915364035 28.074240980532217 27.544424595210277 22.24104550889347 84-85 22.071185238060636 27.85568041444222 27.518689651340228 22.55444469615692 86-87 22.1575387114355 27.744509868878037 27.71056990499802 22.38738151468844 88-89 22.21516958389434 27.768929922420977 27.52490863627621 22.490991857408478 90-91 21.960863596186034 27.850002479043535 27.8685525634165 22.320581361353934 92-93 21.99122640525424 27.876853338531486 27.758868922733885 22.373051333480387 94-95 21.88299722631026 27.8039635252746 27.717149472768128 22.595889775647013 96-97 22.01371812016266 27.926173188274444 27.56513627486858 22.49497241669431 98-99 21.834308166405908 27.929384429152886 27.34781978465731 22.888487619783888 100-101 21.830446867650203 27.920461834509304 27.53401753260637 22.715073765234127 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 299.0 1 210.0 2 153.5 3 188.5 4 194.5 5 227.5 6 363.5 7 654.0 8 1065.0 9 1315.0 10 1278.5 11 1235.0 12 1334.0 13 1445.5 14 1584.0 15 1662.0 16 1717.0 17 1832.0 18 1850.0 19 1942.5 20 2236.5 21 2635.0 22 3114.5 23 3722.5 24 4294.0 25 5261.0 26 6857.5 27 8650.0 28 10196.5 29 11724.0 30 13893.5 31 16437.5 32 19034.5 33 21683.5 34 24856.0 35 27478.0 36 30410.5 37 33646.5 38 34859.5 39 35166.5 40 35928.0 41 37553.0 42 38842.5 43 40214.0 44 42078.0 45 43371.0 46 43292.5 47 43371.5 48 45408.0 49 45578.0 50 43791.5 51 41964.5 52 40111.0 53 39223.0 54 37648.0 55 34293.5 56 30765.5 57 27522.0 58 24791.0 59 21952.5 60 18494.5 61 14694.0 62 11184.5 63 8564.5 64 6401.0 65 4809.5 66 3464.5 67 2547.0 68 1789.5 69 1110.0 70 798.5 71 590.5 72 394.5 73 259.0 74 194.0 75 144.5 76 96.5 77 59.0 78 33.5 79 23.5 80 19.0 81 15.5 82 13.0 83 7.0 84 7.5 85 8.0 86 5.5 87 4.5 88 3.5 89 3.0 90 2.5 91 2.0 92 1.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04128173373025336 2 0.03777748718172253 3 0.025469889547858178 4 0.04512785799083597 5 0.06384566272567134 6 0.06999946154260352 7 0.06495676528983965 8 0.06854648126638342 9 0.06769178698625394 10-11 0.07521309665139328 12-13 0.07397378994520555 14-15 0.06786272584227984 16-17 0.055042311640337814 18-19 0.049486798819496254 20-21 0.05555512820841549 22-23 0.031025402368699728 24-25 0.016410130178485804 26-27 0.014273394478162135 28-29 0.01743576331464117 30-31 0.012264862919857882 32-33 0.007350370809113434 34-35 0.010769147929631311 36-37 0.005940125246899811 38-39 0.008418738659275272 40-41 0.008076860947223484 42-43 0.0053845739648156555 44-45 0.0013675108482071505 46-47 0.0036324506905502435 48-49 0.0010256331361553628 50-51 0.006111064102925704 52-53 0.005299104536802709 54-55 0.0077349832351716954 56-57 0.022521194281411513 58-59 0.009358902367417687 60-61 0.02094000986317199 62-63 0.014700741618226869 64-65 0.01123922978370252 66-67 0.018034049310731798 68-69 0.02619637968596823 70-71 0.01897421301887421 72-73 0.019657968442977788 74-75 0.020469928009100784 76-77 0.026794665682058856 78-79 0.038632181461852 80-81 0.03209377021886157 82-83 0.030640789942641466 84-85 0.020854540435159045 86-87 0.02534168540583876 88-89 0.019999846155029578 90-91 0.017350293886628225 92-93 0.031025402368699728 94-95 0.022863071993463296 96-97 0.039401406313968525 98-99 0.056708965486590275 100-101 0.0648712958618267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1170009.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.27918636033484 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13270385692782 52.60569860001062 2 10.15932305380445 12.857473936330754 3 2.7514394171395677 5.223265429090372 4 1.1898101272905157 3.01160867112921 5 0.6577953198162988 2.081237631480581 6 0.4031750798788398 1.5307554609297591 7 0.27843708777934323 1.233349065705258 8 0.20229568570969644 1.0240885116732485 9 0.16263681865450716 0.9262373001021486 >10 0.9480466762275165 11.300383303013401 >50 0.07891431626848996 3.438077416533667 >100 0.034611358810488414 3.6402277192248103 >500 2.704005641387821E-4 0.0959056287028853 >1k 5.408011282775642E-4 1.0316913260732328 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4951 0.4231591380921001 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3730 0.318800966488292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2367 0.20230613610664533 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.546942801294691E-5 2 0.0 0.0 0.0 8.546942801294691E-5 8.546942801294691E-5 3 0.0 0.0 0.0 8.546942801294691E-5 8.546942801294691E-5 4 0.0 0.0 0.0 8.546942801294691E-5 8.546942801294691E-5 5 0.0 0.0 0.0 1.7093885602589381E-4 8.546942801294691E-5 6 0.0 0.0 0.0 1.7093885602589381E-4 8.546942801294691E-5 7 0.0 0.0 0.0 1.7093885602589381E-4 8.546942801294691E-5 8 0.0 0.0 0.0 1.7093885602589381E-4 8.546942801294691E-5 9 0.0 0.0 0.0 1.7093885602589381E-4 8.546942801294691E-5 10-11 0.0 0.0 0.0 1.7093885602589381E-4 8.546942801294691E-5 12-13 0.0 0.0 0.0 1.7093885602589381E-4 8.546942801294691E-5 14-15 0.0 0.0 0.0 2.1367357003236728E-4 8.546942801294691E-5 16-17 0.0 0.0 0.0 2.991429980453142E-4 8.546942801294691E-5 18-19 0.0 0.0 0.0 3.4187771205178763E-4 8.546942801294691E-5 20-21 0.0 0.0 0.0 3.846124260582611E-4 8.546942801294691E-5 22-23 0.0 0.0 0.0 5.555512820841549E-4 8.546942801294691E-5 24-25 0.0 0.0 0.0 8.974289941359426E-4 8.546942801294691E-5 26-27 0.0 0.0 0.0 0.0016239191322459912 8.546942801294691E-5 28-29 0.0 0.0 0.0 0.0026068175543948807 8.546942801294691E-5 30-31 0.0 0.0 0.0 0.004743553254718554 8.546942801294691E-5 32-33 0.0 0.0 0.0 0.006965758383055173 8.546942801294691E-5 34-35 0.0 0.0 0.0 0.009316167653411212 8.546942801294691E-5 36-37 0.0 0.0 0.0 0.012692210059922616 8.546942801294691E-5 38-39 0.0 0.0 0.0 0.017991314596725323 8.546942801294691E-5 40-41 0.0 0.0 0.0 0.024273317555676924 8.546942801294691E-5 42-43 0.0 0.0 0.0 0.03401683234915287 8.546942801294691E-5 44-45 0.0 0.0 0.0 0.04055524359214331 8.546942801294691E-5 46-47 0.0 0.0 0.0 0.053461127222098295 8.546942801294691E-5 48-49 0.0 0.0 0.0 0.07046954339667472 8.546942801294691E-5 50-51 0.0 0.0 0.0 0.07756350592174932 8.546942801294691E-5 52-53 0.0 0.0 0.0 0.09119587968981435 8.546942801294691E-5 54-55 0.0 0.0 0.0 0.11991360750216451 8.546942801294691E-5 56-57 0.0 0.0 0.0 0.16704999705130474 8.546942801294691E-5 58-59 0.0 0.0 0.0 0.21110948719197886 8.546942801294691E-5 60-61 0.0 0.0 0.0 0.2743141292075531 8.546942801294691E-5 62-63 0.0 0.0 0.0 0.3312795029781822 8.546942801294691E-5 64-65 0.0 0.0 0.0 0.38674916175858476 8.546942801294691E-5 66-67 0.0 0.0 0.0 0.474953611467946 1.2820414201942035E-4 68-69 0.0 0.0 0.0 0.5999099152228744 1.7093885602589381E-4 70-71 0.0 0.0 0.0 0.6560205947133739 1.7093885602589381E-4 72-73 0.0 0.0 0.0 0.7320029162168837 1.7093885602589381E-4 74-75 0.0 0.0 0.0 0.8206347130663098 1.7093885602589381E-4 76-77 0.0 0.0 0.0 0.9056767939391919 1.7093885602589381E-4 78-79 0.0 0.0 0.0 1.0001205118934984 1.7093885602589381E-4 80-81 0.0 0.0 0.0 1.1658029980965958 1.7093885602589381E-4 82-83 0.0 0.0 0.0 1.356912639133545 2.5640828403884075E-4 84-85 0.0 0.0 0.0 1.6365686075919075 2.5640828403884075E-4 86-87 0.0 0.0 0.0 2.034727937990221 2.5640828403884075E-4 88-89 0.0 0.0 0.0 2.4410923334777763 2.5640828403884075E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2985 0.0 39.794193 1 GTATCAA 5230 0.0 39.61036 1 ATCAACG 6530 0.0 31.436323 3 TCAACGC 6620 0.0 31.082048 4 CAACGCA 6705 0.0 30.551498 5 TATCAAC 6695 0.0 30.517004 2 AACGCAG 6905 0.0 29.731606 6 GTACATG 5180 0.0 26.508924 1 ACGCAGA 7875 0.0 25.830254 7 CGCAGAG 7925 0.0 25.609509 8 TACATGG 5510 0.0 24.74881 2 TGGACCG 135 1.3365288E-5 24.644403 5 GCAGAGT 8270 0.0 23.679052 9 ACATGGG 5535 0.0 23.093824 3 GGACCGT 130 2.9321905E-4 21.933413 6 GTGTACG 135 3.7883106E-4 21.11745 1 GTACACG 160 4.977855E-5 20.78749 1 GAGTACT 4400 0.0 20.575918 12-13 AGAGTAC 7475 0.0 20.410229 10-11 AGTACTT 4700 0.0 20.273718 12-13 >>END_MODULE