FastQCFastQC Report
Thu 26 May 2016
SRR936814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170009
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70980.6066620000358971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55450.4739279783317906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35970.30743353256257No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15130.12931524458358867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33400.046.5149541
GTATCAA58950.040.9422041
TATCAAC75850.031.7491652
ATCAACG76500.031.47943
TCAACGC77600.031.1515984
CAACGCA79100.030.5008185
AACGCAG81000.029.7853666
ACGCAGA93200.025.887537
CGCAGAG93650.025.6109928
GGACCGA1501.0616168E-625.3292876
GTACATG52700.024.5216451
TACATGG53800.023.9258272
GCAGAGT99050.023.7352359
ACATGGG53350.023.0592563
GAGTACT59700.022.15753712-13
AGTACTT61200.021.73087712-13
CATGGGG23350.021.5597274
AGAGTAC90500.020.88705810-11
GTACTTT63100.020.77633514-15
TACATCG1200.004767952519.7885065
Produced by FastQC (version 0.11.5)