##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936814_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1170009 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2945883322265 33.0 31.0 34.0 31.0 34.0 2 32.36507582420306 33.0 31.0 34.0 31.0 34.0 3 32.49502781602535 34.0 31.0 34.0 31.0 34.0 4 36.15210652225752 37.0 35.0 37.0 35.0 37.0 5 36.07699257014262 37.0 35.0 37.0 35.0 37.0 6 36.11471364750186 37.0 35.0 37.0 35.0 37.0 7 36.073372085172 37.0 35.0 37.0 35.0 37.0 8 36.0566525556641 37.0 35.0 37.0 35.0 37.0 9 37.7678966572052 39.0 37.0 39.0 35.0 39.0 10-11 37.674219600020166 39.0 37.0 39.0 35.0 39.0 12-13 37.60239194741237 39.0 37.0 39.0 35.0 39.0 14-15 38.86057842290102 40.0 38.0 41.0 35.0 41.0 16-17 38.78357388703847 40.0 38.0 41.0 35.0 41.0 18-19 38.82342144376668 40.0 38.0 41.0 35.0 41.0 20-21 38.91360151930455 40.0 38.0 41.0 35.0 41.0 22-23 38.84914603221001 40.0 38.0 41.0 35.0 41.0 24-25 38.76941502159385 40.0 38.0 41.0 35.0 41.0 26-27 38.600134699818554 40.0 38.0 41.0 34.0 41.0 28-29 38.44740040461227 40.0 38.0 41.0 34.0 41.0 30-31 38.3918692078437 40.0 38.0 41.0 34.0 41.0 32-33 38.28977939485935 40.0 38.0 41.0 34.0 41.0 34-35 38.16853972918157 40.0 38.0 41.0 34.0 41.0 36-37 38.00090939471406 40.0 37.0 41.0 33.5 41.0 38-39 37.86259293731929 40.0 37.0 41.0 33.0 41.0 40-41 37.642367708282585 40.0 37.0 41.0 33.0 41.0 42-43 37.4126058004682 40.0 37.0 41.0 33.0 41.0 44-45 37.13098873598408 40.0 37.0 41.0 32.0 41.0 46-47 36.721085906176796 40.0 36.0 41.0 31.0 41.0 48-49 36.31474373274052 39.5 36.0 41.0 30.0 41.0 50-51 36.06445933321881 39.0 36.0 41.0 29.5 41.0 52-53 35.83728073886611 39.0 35.0 40.5 29.0 41.0 54-55 35.56892041001394 39.0 35.0 40.0 28.0 41.0 56-57 35.329330372672345 38.5 35.0 40.0 28.0 41.0 58-59 34.994526537830055 38.0 34.5 40.0 27.0 41.0 60-61 34.669594849270396 38.0 34.0 40.0 26.0 41.0 62-63 34.35346138363038 37.5 34.0 40.0 26.0 41.0 64-65 33.95487641548056 37.0 33.5 39.5 25.5 41.0 66-67 33.53319718053451 36.0 33.0 39.0 24.5 40.5 68-69 33.10460432355649 36.0 33.0 39.0 23.0 40.0 70-71 32.713371435604344 35.0 32.0 38.0 22.0 40.0 72-73 32.32040907377636 35.0 32.0 38.0 21.5 40.0 74-75 31.84967508796941 35.0 31.5 37.0 20.0 39.0 76-77 30.40540841993523 33.5 29.5 35.5 19.5 38.0 78-79 31.08747710487697 34.0 31.0 36.0 19.0 38.5 80-81 30.749443807697205 34.0 31.0 36.0 14.0 37.5 82-83 30.58930444124789 34.0 31.0 35.0 17.0 37.0 84-85 30.281935865450606 34.0 31.0 35.0 13.0 36.5 86-87 29.92931293690903 34.0 30.0 35.0 7.5 36.0 88-89 29.583196795922085 34.0 30.0 35.0 4.5 36.0 90-91 29.161040641567716 34.0 29.5 35.0 2.0 35.0 92-93 28.770322279572206 34.0 29.0 35.0 2.0 35.0 94-95 28.313460836626042 34.0 29.0 35.0 2.0 35.0 96-97 27.718756436916298 33.5 28.0 35.0 2.0 35.0 98-99 26.98770821421032 33.0 26.5 35.0 2.0 35.0 100-101 23.833010686242584 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2481110183487445 1101 2 0.16806737540959205 1101 3 0.14028636195157418 1101 4 0.04532733871141659 1101 5 0.11363664621443093 1101 6 0.1914052551288563 1101 7 0.14785357652565523 1101 8 0.19147994629243925 1101 9 0.26796844253347984 1101 10-11 0.2371360354486427 1101 12-13 0.2055190974788914 1101 14-15 0.3119724981798484 1101 16-17 0.30980438849884706 1101 18-19 0.2627956194696921 1101 20-21 0.1011600610117469 1101 22-23 0.24447992136065722 1101 24-25 0.26140044456055733 1101 26-27 0.21735419797174416 1101 28-29 0.22301514927383437 1101 30-31 0.1531623211243769 1101 32-33 0.22380538435766084 1101 34-35 0.30918585738078264 1101 36-37 0.33370151261744496 1101 38-39 0.361338032329094 1101 40-41 0.5186325915479486 1101 42-43 0.4967435300327452 1101 44-45 0.5158726361218271 1101 46-47 0.5647126357982941 1101 48-49 0.48650418212960034 1101 50-51 0.45726625932863385 1101 52-53 0.3058748717670383 1101 54-55 0.46637432815745683 1101 56-57 0.3799431410368541 1101 58-59 0.5055084715387821 1101 60-61 0.3817392398984012 1101 62-63 0.2985497271923805 1101 64-65 0.44507384813013573 1101 66-67 0.4170748450858923 1101 68-69 0.39917212072812447 1101 70-71 0.4246490976789019 1101 72-73 0.3926406639225064 1101 74-75 0.32449360461058774 1101 76-77 0.3634656435422805 1101 78-79 0.507411167646115 1101 80-81 0.7774567578058935 1101 82-83 0.5495539071266649 1101 84-85 0.4636526835398236 1101 86-87 0.39360764998501807 1101 88-89 0.24168231841920118 1101 90-91 0.043338716690652035 1101 92-93 0.39626750610377925 1101 94-95 0.386289721347552 1101 96-97 0.24700602382739945 1101 98-99 0.44645247885494754 1101 100-101 0.8280285621185399 1102 1 0.19081797125978284 1102 2 0.206871156662352 1102 3 0.1452956383894204 1102 4 0.09713580235661112 1102 5 0.20045880364882862 1102 6 0.2065497025182026 1102 7 0.17965320546814212 1102 8 0.2031929951483491 1102 9 0.21767954775479126 1102 10-11 0.2311116516691598 1102 12-13 0.2771782546482555 1102 14-15 0.19686515654188952 1102 16-17 0.3289262190492437 1102 18-19 0.31931651354160806 1102 20-21 0.29514981275659835 1102 22-23 0.27807211148346056 1102 24-25 0.16903186076340404 1102 26-27 0.20976253793109834 1102 28-29 0.2669192044023774 1102 30-31 0.28188393964190794 1102 32-33 0.13816000932453676 1102 34-35 0.22342474774212207 1102 36-37 0.2801959025812266 1102 38-39 0.17491166957620408 1102 40-41 0.21005586215488847 1102 42-43 0.16867479604565006 1102 44-45 0.2043336309093533 1102 46-47 0.30501743304186846 1102 48-49 0.3852505378147342 1102 50-51 0.3557952813270475 1102 52-53 0.3872053779955067 1102 54-55 0.30513128548583524 1102 56-57 0.5140026869331322 1102 58-59 0.5226530956221822 1102 60-61 0.46056775831875285 1102 62-63 0.5004730419728389 1102 64-65 0.5527229337384085 1102 66-67 0.5578037080596516 1102 68-69 0.4912685956817455 1102 70-71 0.5560714848155683 1102 72-73 0.5420080992083101 1102 74-75 0.5917292261319247 1102 76-77 0.6373308259788928 1102 78-79 0.48458317930792205 1102 80-81 0.5983941197636611 1102 82-83 0.5748476609918463 1102 84-85 0.6464780214671997 1102 86-87 0.5442393312289475 1102 88-89 0.4605859318126093 1102 90-91 0.4118076627106184 1102 92-93 0.5125931532457564 1102 94-95 0.7225385065759262 1102 96-97 0.8938670539537377 1102 98-99 0.9080188716458295 1102 100-101 1.1413557111599744 1103 1 0.18489536402712758 1103 2 0.18003473253335045 1103 3 0.12821970122614346 1103 4 0.12130223272391305 1103 5 0.14133216609424437 1103 6 0.12198439882626388 1103 7 0.14628508886858071 1103 8 0.12578170192919202 1103 9 0.20606155875688614 1103 10-11 0.15779627708740662 1103 12-13 0.20836580072663224 1103 14-15 0.22520406654019354 1103 16-17 0.18707022933299555 1103 18-19 0.3548831594139301 1103 20-21 0.2610363134465885 1103 22-23 0.32214043035526885 1103 24-25 0.19074720509883036 1103 26-27 0.2993356867618928 1103 28-29 0.26917350032479703 1103 30-31 0.40115651568304855 1103 32-33 0.2715882404820178 1103 34-35 0.3899464211196957 1103 36-37 0.49939721073450016 1103 38-39 0.3808486438101184 1103 40-41 0.48178840908393994 1103 42-43 0.3482757336815183 1103 44-45 0.4568111824698704 1103 46-47 0.5458856914210486 1103 48-49 0.4268787349451131 1103 50-51 0.4787947709204161 1103 52-53 0.24652823854107453 1103 54-55 0.20794816293153673 1103 56-57 0.2589124205764932 1103 58-59 0.44220242547718414 1103 60-61 0.21652987480489116 1103 62-63 0.24266344254751715 1103 64-65 0.23418609939371038 1103 66-67 0.181028910804514 1103 68-69 0.2814642515542545 1103 70-71 0.19110485028561897 1103 72-73 0.35788949583206175 1103 74-75 0.2766903785166619 1103 76-77 0.4022946249164896 1103 78-79 0.45902485117317227 1103 80-81 0.742850725748653 1103 82-83 0.48919386791447295 1103 84-85 0.26479594808207096 1103 86-87 0.26334933330837984 1103 88-89 0.34276574617582867 1103 90-91 0.37585046125123256 1103 92-93 0.42406614895455874 1103 94-95 0.5864603198427147 1103 96-97 0.4975019052741736 1103 98-99 0.6851872491198847 1103 100-101 0.9000364045568254 1104 1 0.13050248765662786 1104 2 0.042479292818434544 1104 3 0.07319103066837584 1104 4 0.11591122784436436 1104 5 -0.1150206484059666 1104 6 0.0027016327797255713 1104 7 0.06697574594136313 1104 8 0.1025786323422011 1104 9 0.05907431736873292 1104 10-11 0.06340305889207087 1104 12-13 0.19975608777403409 1104 14-15 0.1319523296427647 1104 16-17 0.12827657189274788 1104 18-19 -0.0041309750428695224 1104 20-21 0.09113569901645491 1104 22-23 0.11491104880185077 1104 24-25 0.11122571713533347 1104 26-27 0.2545861161793397 1104 28-29 0.15894909232267906 1104 30-31 0.30007613557383195 1104 32-33 0.22812264942415794 1104 34-35 0.17545048456238277 1104 36-37 0.26615688224083556 1104 38-39 0.2580831100079166 1104 40-41 0.2576087886804217 1104 42-43 0.2317549122764433 1104 44-45 0.4361040750770613 1104 46-47 0.3534981304266793 1104 48-49 0.5589491679517167 1104 50-51 0.3549858168189459 1104 52-53 0.3541605461150539 1104 54-55 0.18009392558546722 1104 56-57 0.11564444699954635 1104 58-59 0.22643682331001713 1104 60-61 0.3169563012826373 1104 62-63 0.24498528771796657 1104 64-65 0.22898694370104522 1104 66-67 0.18013247518294406 1104 68-69 0.4246683466157464 1104 70-71 0.17060241051087388 1104 72-73 0.10880344997625713 1104 74-75 0.058285888647489514 1104 76-77 0.012557060931154496 1104 78-79 0.1620489327325778 1104 80-81 0.3189150828188687 1104 82-83 0.08137526580712162 1104 84-85 -0.04265647417414087 1104 86-87 0.10873704812392404 1104 88-89 0.2043924905506742 1104 90-91 0.13365282667906797 1104 92-93 0.27059854967580677 1104 94-95 0.25838325543437435 1104 96-97 0.4716296110313607 1104 98-99 0.43904004225604254 1104 100-101 0.46008755009729185 1105 1 0.12843379895825535 1105 2 0.12389094128544542 1105 3 0.08165927475305779 1105 4 0.10756906055846827 1105 5 0.14406511469315575 1105 6 0.10138065668800067 1105 7 0.11304163522020616 1105 8 0.1476307612390073 1105 9 0.15605670778484182 1105 10-11 0.10347078014398647 1105 12-13 0.14375225915614465 1105 14-15 0.10024607314594647 1105 16-17 0.11448093981058349 1105 18-19 0.17771370863302138 1105 20-21 0.20562063669849096 1105 22-23 -0.02628801874443809 1105 24-25 0.16628705735743665 1105 26-27 0.18429547056320672 1105 28-29 0.11346315631823245 1105 30-31 0.07109005033561999 1105 32-33 0.04513221067689699 1105 34-35 0.17003381360738246 1105 36-37 0.1451370495884916 1105 38-39 0.22638174220330143 1105 40-41 0.24891386365752055 1105 42-43 0.42511606827204673 1105 44-45 0.34862864718783726 1105 46-47 0.36107303033713833 1105 48-49 0.5424331073113464 1105 50-51 0.24982232865987442 1105 52-53 0.2625472262299198 1105 54-55 0.22443256272099887 1105 56-57 -0.00819616105084009 1105 58-59 0.2194174332129606 1105 60-61 0.34949337815346837 1105 62-63 0.10851461472515211 1105 64-65 0.1373810855039963 1105 66-67 0.08846486508444684 1105 68-69 0.0619973710386148 1105 70-71 0.18214461278952143 1105 72-73 0.1400972552313462 1105 74-75 -0.13070503382299847 1105 76-77 -0.13746506683353132 1105 78-79 -0.02682929251777466 1105 80-81 0.23845672933040163 1105 82-83 0.07985642633119028 1105 84-85 -0.22797727530214473 1105 86-87 -0.15638676443846933 1105 88-89 -0.19956683553024135 1105 90-91 -0.01736839539105972 1105 92-93 0.42132593561489884 1105 94-95 0.25840126640562744 1105 96-97 0.37827977621769904 1105 98-99 0.41265448396739046 1105 100-101 0.5716462195496561 1106 1 0.08296465618997217 1106 2 0.051248154235224774 1106 3 0.14762423762523724 1106 4 0.09228299193679135 1106 5 0.05861899397397252 1106 6 0.045985936387431536 1106 7 0.0028051051148878514 1106 8 0.11540167993435091 1106 9 0.1023439555119765 1106 10-11 0.13209283543384487 1106 12-13 0.07973827195053929 1106 14-15 0.2250462537951492 1106 16-17 0.15937631877769576 1106 18-19 0.0889385066687538 1106 20-21 0.024306806965135763 1106 22-23 0.07629117764066962 1106 24-25 0.004951885755588137 1106 26-27 0.07223844690184933 1106 28-29 0.1349352090163194 1106 30-31 0.0010774608972852207 1106 32-33 0.026191153438148262 1106 34-35 0.06653170950887954 1106 36-37 -0.031711193758859224 1106 38-39 0.07752191156788513 1106 40-41 0.16957716559371505 1106 42-43 0.0573939463111941 1106 44-45 0.12861138065102296 1106 46-47 0.19067888499198204 1106 48-49 0.2016740112485138 1106 50-51 0.12545500304862145 1106 52-53 0.13284592301749143 1106 54-55 0.2865089799563165 1106 56-57 0.10377655567607036 1106 58-59 0.30019569048436523 1106 60-61 0.2738876343190455 1106 62-63 0.34465178419281983 1106 64-65 0.2837358383595614 1106 66-67 0.17367206650533262 1106 68-69 0.25348761377705387 1106 70-71 0.3749816811484479 1106 72-73 0.32368618057711274 1106 74-75 0.35144662213798483 1106 76-77 0.05139229715376459 1106 78-79 0.20433734267033188 1106 80-81 0.603622533961147 1106 82-83 0.5503841519922545 1106 84-85 0.32523946620802846 1106 86-87 0.19303658208805174 1106 88-89 -5.101199019996727E-4 1106 90-91 0.11765199020442907 1106 92-93 0.14370290260292862 1106 94-95 0.22695299243370926 1106 96-97 0.23977242006528954 1106 98-99 0.2686251725614106 1106 100-101 0.4258857858962344 1107 1 0.07753016360577192 1107 2 0.018079009842033145 1107 3 -0.060039402156888855 1107 4 -0.04684200387471549 1107 5 -0.022764983518619886 1107 6 -0.05690455840084496 1107 7 0.08590729566589062 1107 8 0.15182295673605495 1107 9 0.0018337659935170336 1107 10-11 0.09990379848947128 1107 12-13 0.12081746363957535 1107 14-15 0.1506067983117063 1107 16-17 0.12867155978050704 1107 18-19 0.08236815673409126 1107 20-21 0.08957718507129897 1107 22-23 0.12329476050892652 1107 24-25 0.036597659596800725 1107 26-27 0.12994037261597668 1107 28-29 -0.01978587455622005 1107 30-31 0.0537789460439555 1107 32-33 0.25981600591011045 1107 34-35 0.05482750773642664 1107 36-37 0.02320771080430717 1107 38-39 0.06654774867706692 1107 40-41 0.2719039202001312 1107 42-43 0.13597597686795382 1107 44-45 0.0840476727360695 1107 46-47 0.3638792910586588 1107 48-49 0.34650249399626176 1107 50-51 0.3499694170504739 1107 52-53 0.14182481176986528 1107 54-55 0.29304706297907757 1107 56-57 0.43117867213728545 1107 58-59 0.36267870381680467 1107 60-61 0.47916073029200845 1107 62-63 0.4857810516470451 1107 64-65 0.3523914498858005 1107 66-67 0.28415738568094184 1107 68-69 0.3883037358154695 1107 70-71 0.3443386636461341 1107 72-73 0.26672142969628254 1107 74-75 0.1829171618269001 1107 76-77 0.2536304418559716 1107 78-79 0.3576504719526774 1107 80-81 0.6019949164630489 1107 82-83 0.508799859028553 1107 84-85 0.3795694996291026 1107 86-87 0.5046462169879469 1107 88-89 0.27297460729987577 1107 90-91 0.09528326379185259 1107 92-93 0.32760382332684657 1107 94-95 0.5223820120532352 1107 96-97 0.5479828599005252 1107 98-99 0.71806012929272 1107 100-101 0.6280109964068075 1108 1 0.015424651876692508 1108 2 0.0027217340474550156 1108 3 0.06817403876142691 1108 4 0.07666459411046844 1108 5 -0.08255909916447024 1108 6 -0.03507937599066935 1108 7 -0.013292621083827783 1108 8 0.08530878217462146 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66-67 -0.6745527423504853 2316 68-69 -1.0598167544640091 2316 70-71 -0.8250640314206166 2316 72-73 -0.8046673804201916 2316 74-75 -0.7878897827488309 2316 76-77 -0.9023152997074995 2316 78-79 -0.7701446357554644 2316 80-81 -0.476252344882802 2316 82-83 -0.5094517085424215 2316 84-85 -1.0518407365830988 2316 86-87 -0.9342841587039388 2316 88-89 -1.042968403486693 2316 90-91 -0.9904972657688056 2316 92-93 -0.9945595821620152 2316 94-95 -0.9046799870855224 2316 96-97 -1.176886115322926 2316 98-99 -1.1701066791642845 2316 100-101 -1.3837917364706946 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 6.0 5 22.0 6 46.0 7 118.0 8 294.0 9 520.0 10 945.0 11 1626.0 12 2600.0 13 3718.0 14 4827.0 15 6737.0 16 9099.0 17 13532.0 18 12437.0 19 6959.0 20 5487.0 21 5525.0 22 5716.0 23 5919.0 24 6865.0 25 8199.0 26 9860.0 27 12390.0 28 15371.0 29 19558.0 30 24537.0 31 31293.0 32 40389.0 33 53202.0 34 74851.0 35 109568.0 36 173157.0 37 273645.0 38 213412.0 39 17574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.172017823129714 19.31939034774253 15.163082320836851 22.3455095082909 2 15.610837682641005 20.745556526183886 40.232734260007966 23.41087153116714 3 19.256836748038918 25.107296320768345 30.4561707030826 25.17969622811014 4 12.90854421508229 17.459196860192407 37.44948752487163 32.18277139985368 5 13.614568019419467 37.37016650141885 34.11236281582276 14.902902663338919 6 31.194888244449402 36.64783775167542 17.602172290982377 14.555101712892807 7 27.97952836260234 33.371196289943065 20.794711835549982 17.854563511904608 8 26.36048098775309 34.525033568117856 19.9042913345111 19.21019410961796 9 26.613043147531346 16.74636690828874 20.511466151115076 36.129123793064835 10-11 24.447119637541253 27.172739696874125 27.301584859603643 21.07855580598098 12-13 25.92014406751413 24.43361449974231 28.11560101777169 21.530640414971877 14-15 22.796961390895284 25.52971814746724 26.9758181347323 24.697502326905177 16-17 21.64343250497753 28.849405794915167 27.44723649443572 22.059925205671583 18-19 21.417694946517194 26.79456611811304 30.754716739226406 21.033022196143357 20-21 22.186884742884224 25.948289235321543 30.951287128035876 20.913538893758357 22-23 21.917951058496133 25.961680636644672 30.883223975200192 21.237144329659003 24-25 21.88680874123573 26.082760872482474 30.9192110083431 21.1112193779387 26-27 21.73825710159706 26.308827746605463 30.70058435095076 21.252330800846718 28-29 21.333485480618744 26.308017905513115 30.982144917725073 21.376351696143068 30-31 21.58231925355863 26.148061418031705 30.930020907847734 21.339598420561934 32-33 21.55883360982835 26.25937711270826 30.76932281370219 21.412466463761206 34-35 21.81991536470019 26.260191579035542 30.640337583902372 21.2795554723619 36-37 21.80713426607022 26.026462213385148 30.567121922280283 21.599281598264348 38-39 21.524362021342387 26.343728724606823 30.783199443041457 21.348709811009332 40-41 21.708901117875236 26.18894980588051 30.69951397472258 21.40263510152167 42-43 21.687415351647793 26.20450144391017 30.350064342363776 21.758018862078266 44-45 21.887708615747016 26.387124842090508 30.026778372852732 21.698388169309744 46-47 21.950589943712927 26.778474415686702 29.474535773764526 21.79639986683584 48-49 22.121428000603427 27.02174514128594 28.992667003138074 21.864159854972563 50-51 22.12867796320434 27.095322549627127 28.82963311181863 21.946366375349903 52-53 22.335867742882577 27.160419961819322 28.623200638310507 21.88051165698759 54-55 22.073250574366828 27.113148555877686 28.680264003610308 22.13333686614518 56-57 22.18499518802596 27.559876170728497 28.440176820815076 21.814951820430462 58-59 22.297270154607762 27.060546516090266 28.66170994185435 21.980473387447617 60-61 22.212958281927012 26.919035203590404 29.11607052427397 21.75193599020862 62-63 22.064839699882523 27.01963276335172 29.161711218337306 21.753816318428456 64-65 21.980472135653955 27.063178786889335 28.876211837068922 22.08013724038779 66-67 21.88932027632538 27.41215259006964 28.579932863104407 22.118594270500576 68-69 22.00201197784944 27.081444077015842 28.944275734983027 21.972268210151686 70-71 22.0045113000459 27.206823191740508 28.793983679658382 21.994681828555212 72-73 22.035415931185458 27.10846257145031 28.582601983640902 22.27351951372333 74-75 22.0054028203902 27.279114522099125 28.391304031943392 22.32417862556728 76-77 22.156227817168812 27.33638499287799 28.3775704305558 22.1298167593974 78-79 22.065018189775433 27.313811236631196 28.314590798435407 22.306579775157964 80-81 22.13317709481832 27.461183959740374 28.152889814240268 22.252749131201035 82-83 22.106015777617564 27.456165805588878 28.25675660082474 22.18106181596882 84-85 21.90567847124525 27.262397029738512 28.20209949076836 22.629825008247877 86-87 22.061368758701853 27.14316727478165 28.33956832810688 22.455895638409622 88-89 21.96723483270192 27.12735338047039 28.215095395910655 22.690316390917037 90-91 21.807592061015463 27.09396635978593 28.59358366698761 22.504857912210998 92-93 21.719504002543577 26.921229463780527 28.606947314800095 22.7523192188758 94-95 21.613989383774292 27.070178969856023 28.495177142935287 22.820654503434397 96-97 21.830133623703208 26.95115051085383 28.363583993873515 22.85513187156945 98-99 21.637599839316927 26.920440510937986 28.260819398207698 23.181140251537386 100-101 21.312380555147197 27.03509242766642 28.2862078160951 23.366319201091283 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 375.0 1 291.5 2 217.0 3 239.0 4 224.0 5 220.0 6 404.5 7 905.5 8 1519.0 9 1798.0 10 1599.0 11 1442.5 12 1563.0 13 1713.0 14 1871.5 15 2053.0 16 2166.5 17 2222.5 18 2341.5 19 2541.0 20 2775.5 21 3051.5 22 3480.5 23 4060.0 24 4778.0 25 5822.5 26 7232.0 27 8854.0 28 10386.5 29 12109.5 30 14303.0 31 16559.5 32 18815.0 33 21025.5 34 24218.5 35 27053.5 36 30066.5 37 33170.0 38 34476.0 39 34970.0 40 35809.0 41 37211.0 42 38429.5 43 40042.0 44 41449.5 45 42505.5 46 43029.0 47 43361.5 48 44966.0 49 45061.0 50 43218.0 51 41754.5 52 39940.5 53 38907.0 54 37220.0 55 33725.0 56 30353.5 57 27284.5 58 24842.5 59 22076.0 60 18651.0 61 14818.5 62 11302.5 63 8717.0 64 6428.5 65 4688.0 66 3415.0 67 2603.5 68 1847.0 69 1139.5 70 773.0 71 518.0 72 353.5 73 242.0 74 187.0 75 156.0 76 113.0 77 57.5 78 27.0 79 15.5 80 11.5 81 8.5 82 5.5 83 4.0 84 3.0 85 3.0 86 1.5 87 1.0 88 1.5 89 1.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025042542407793442 2 0.007777717949178169 3 0.010085392505527735 4 8.546942801294691E-5 5 0.004188001972634399 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.7093885602589381E-4 14-15 0.0 16-17 4.2734714006473454E-5 18-19 0.00457261439869266 20-21 0.0017521232742654116 22-23 0.0 24-25 0.005128165680776815 26-27 0.006709350099016333 28-29 0.007692248521165223 30-31 0.03012797337456379 32-33 5.128165680776814E-4 34-35 0.004102532544621451 36-37 0.016239191322459913 38-39 0.0071366972390810674 40-41 0.009487106509437106 42-43 0.00816233037523643 44-45 0.0029914299804531417 46-47 0.0020940009863171993 48-49 0.0020085315583042523 50-51 0.002905960552440195 52-53 0.0038888589745890846 54-55 0.0021367357003236726 56-57 9.401637081424161E-4 58-59 0.0013675108482071505 60-61 4.2734714006473454E-5 62-63 4.2734714006473454E-5 64-65 0.007777717949178169 66-67 9.828984221488895E-4 68-69 0.0013675108482071505 70-71 0.004957226824750921 72-73 0.011965719921812567 74-75 0.005512778106835075 76-77 0.0038033895465761376 78-79 0.010341800789566577 80-81 2.564082840388407E-4 82-83 0.0051709003947832885 84-85 9.401637081424161E-4 86-87 0.0 88-89 0.001410245562213624 90-91 8.974289941359425E-4 92-93 2.564082840388407E-4 94-95 6.410207100971018E-4 96-97 5.982859960906283E-4 98-99 3.4187771205178763E-4 100-101 0.0011111025641683098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1170009.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.75897472620247 #Duplication Level Percentage of deduplicated Percentage of total 1 82.09556716826457 49.88042490709844 2 10.555039762600058 12.826267883397582 3 3.0403239177574823 5.541808922354872 4 1.3189513596815032 3.205525293119153 5 0.6983986114363415 2.1216991790537785 6 0.4445612777495707 1.6206652463420665 7 0.3126178261408647 1.3296037018217208 8 0.21770062285859615 1.058181331371518 9 0.16701424372718573 0.9132852792182308 >10 1.0202358122640827 11.661497994109746 >50 0.08534141254265731 3.571962519404787 >100 0.04297973256663475 4.470122549246211 >500 7.045846724833456E-4 0.2789489324781802 >1k 2.818338689933382E-4 0.4375165883865871 >5k 2.818338689933382E-4 1.082489672597186 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7098 0.6066620000358971 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5545 0.4739279783317906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3597 0.30743353256257 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1513 0.12931524458358867 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.2734714006473454E-5 0.0 16-17 0.0 0.0 0.0 8.546942801294691E-5 0.0 18-19 0.0 0.0 0.0 1.7093885602589381E-4 0.0 20-21 0.0 8.546942801294691E-5 0.0 3.4187771205178763E-4 0.0 22-23 0.0 8.546942801294691E-5 0.0 5.982859960906284E-4 0.0 24-25 0.0 8.546942801294691E-5 0.0 9.401637081424161E-4 0.0 26-27 0.0 8.546942801294691E-5 0.0 0.001581184418239518 0.0 28-29 0.0 8.546942801294691E-5 0.0 0.0026068175543948807 0.0 30-31 0.0 8.546942801294691E-5 0.0 0.0047862879687250275 0.0 32-33 0.0 8.546942801294691E-5 0.0 0.00717943195308754 0.0 34-35 0.0 8.546942801294691E-5 0.0 0.009572575937450053 0.0 36-37 0.0 8.546942801294691E-5 0.0 0.012948618343961457 0.0 38-39 0.0 8.546942801294691E-5 0.0 0.018333192308777113 0.0 40-41 0.0 8.546942801294691E-5 0.0 0.02435878698368987 0.0 42-43 0.0 8.546942801294691E-5 0.0 0.03414503649117229 0.0 44-45 0.0 8.546942801294691E-5 0.0 0.040640713020156254 0.0 46-47 0.0 8.546942801294691E-5 0.0 0.05380300493415008 0.0 48-49 0.0 8.546942801294691E-5 0.0 0.070854155822733 0.0 50-51 0.0 8.546942801294691E-5 0.0 0.0777771794917817 0.0 52-53 0.0 8.546942801294691E-5 0.0 0.09102494083378845 8.546942801294691E-5 54-55 0.0 8.546942801294691E-5 0.0 0.11927258679206741 8.546942801294691E-5 56-57 0.0 8.546942801294691E-5 0.0 0.1666653846252465 8.546942801294691E-5 58-59 0.0 8.546942801294691E-5 0.0 0.2115368343320436 8.546942801294691E-5 60-61 0.0 8.546942801294691E-5 0.0 0.2746560069196049 8.546942801294691E-5 62-63 0.0 8.546942801294691E-5 0.0 0.3314077071202016 8.546942801294691E-5 64-65 0.0 8.546942801294691E-5 0.0 0.38674916175858476 8.546942801294691E-5 66-67 0.0 8.546942801294691E-5 0.0 0.47512455032397183 8.546942801294691E-5 68-69 0.0 8.546942801294691E-5 0.0 0.6004654665049585 8.546942801294691E-5 70-71 0.0 8.546942801294691E-5 0.0 0.6561487988553933 8.546942801294691E-5 72-73 0.0 8.546942801294691E-5 0.0 0.7310200177947349 8.546942801294691E-5 74-75 0.0 8.546942801294691E-5 0.0 0.8183270385099601 1.2820414201942035E-4 76-77 0.0 8.546942801294691E-5 0.0 0.9024716903887064 1.7093885602589381E-4 78-79 0.0 8.546942801294691E-5 0.0 0.9958043057788445 1.7093885602589381E-4 80-81 0.0 8.546942801294691E-5 0.0 1.159136382711586 2.1367357003236728E-4 82-83 0.0 8.546942801294691E-5 0.0 1.3495622683244317 2.5640828403884075E-4 84-85 0.0 8.546942801294691E-5 0.0 1.6284917466446838 2.5640828403884075E-4 86-87 0.0 8.546942801294691E-5 0.0 2.026822015899023 2.5640828403884075E-4 88-89 0.0 8.546942801294691E-5 0.0 2.4344684528067733 2.5640828403884075E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3340 0.0 46.514954 1 GTATCAA 5895 0.0 40.942204 1 TATCAAC 7585 0.0 31.749165 2 ATCAACG 7650 0.0 31.4794 3 TCAACGC 7760 0.0 31.151598 4 CAACGCA 7910 0.0 30.500818 5 AACGCAG 8100 0.0 29.785366 6 ACGCAGA 9320 0.0 25.88753 7 CGCAGAG 9365 0.0 25.610992 8 GGACCGA 150 1.0616168E-6 25.329287 6 GTACATG 5270 0.0 24.521645 1 TACATGG 5380 0.0 23.925827 2 GCAGAGT 9905 0.0 23.735235 9 ACATGGG 5335 0.0 23.059256 3 GAGTACT 5970 0.0 22.157537 12-13 AGTACTT 6120 0.0 21.730877 12-13 CATGGGG 2335 0.0 21.559727 4 AGAGTAC 9050 0.0 20.887058 10-11 GTACTTT 6310 0.0 20.776335 14-15 TACATCG 120 0.0047679525 19.788506 5 >>END_MODULE