FastQCFastQC Report
Thu 26 May 2016
SRR936813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253013
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53630.42800832872444255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40310.32170456332057207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23970.19129889314795617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32550.042.471861
GTATCAA56900.040.4103281
TATCACG550.002576791734.553442
ATCAACG70400.032.4613343
TCAACGC71350.032.0291254
TATCAAC73100.031.3273492
CAACGCA73300.031.241875
AACGCAG74700.030.7835546
ACGCAGA83550.027.0678947
CGCAGAG83250.026.9942288
GCAGAGT88900.024.6363189
GTACATG59200.024.0745891
GAGTACT45500.023.49209612-13
TACATGG61900.022.3355332
ACATGGG60900.021.844133
GTACTTT49500.020.82388914-15
AGTACTT48000.020.7839812-13
AGAGTAC81100.020.53218510-11
TGTACCG1908.235758E-620.0046225
CAGAGTA87050.019.94741210-11
Produced by FastQC (version 0.11.5)