##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936813_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1253013 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.883816847869895 33.0 31.0 34.0 30.0 34.0 2 32.00846439741647 33.0 31.0 34.0 30.0 34.0 3 32.01263514424831 34.0 31.0 34.0 30.0 34.0 4 35.57644653327619 37.0 35.0 37.0 33.0 37.0 5 35.57374025648576 37.0 35.0 37.0 33.0 37.0 6 35.676287476666246 37.0 35.0 37.0 33.0 37.0 7 35.61937266413038 37.0 35.0 37.0 33.0 37.0 8 35.57697725402689 37.0 35.0 37.0 33.0 37.0 9 37.2119140024884 39.0 37.0 39.0 34.0 39.0 10-11 37.091486281467155 39.0 37.0 39.0 33.0 39.0 12-13 37.00351712232833 39.0 37.0 39.0 33.0 39.0 14-15 38.2008211407224 40.0 38.0 41.0 33.0 41.0 16-17 38.12977479084415 40.0 38.0 41.0 33.0 41.0 18-19 38.07365885270144 40.0 38.0 41.0 33.0 41.0 20-21 37.97741124792799 40.0 38.0 41.0 33.0 41.0 22-23 37.849069802148904 40.0 38.0 41.0 33.0 41.0 24-25 37.96654264560703 40.0 38.0 41.0 33.0 41.0 26-27 37.92100361289149 40.0 38.0 41.0 33.0 41.0 28-29 37.82394077316037 40.0 38.0 41.0 33.0 41.0 30-31 37.650733073000836 40.0 37.0 41.0 32.5 41.0 32-33 37.45821152693547 40.0 37.0 41.0 32.0 41.0 34-35 37.27622019883273 40.0 36.5 41.0 31.5 41.0 36-37 37.09429750529324 40.0 36.0 41.0 31.0 41.0 38-39 36.940066862833824 39.0 36.0 41.0 31.0 41.0 40-41 36.75920521175758 39.0 36.0 41.0 30.5 41.0 42-43 36.53314091713334 39.0 35.5 41.0 30.0 41.0 44-45 36.264114977258814 39.0 35.0 41.0 30.0 41.0 46-47 35.905924758961 39.0 35.0 40.0 28.5 41.0 48-49 35.664258471380585 39.0 35.0 40.0 28.0 41.0 50-51 34.967802010035015 38.0 34.0 40.0 27.0 40.5 52-53 35.16421058680157 38.5 35.0 40.0 27.5 40.5 54-55 35.56033895897329 39.0 35.0 40.5 28.0 41.0 56-57 35.47053781564916 39.0 35.0 41.0 27.5 41.0 58-59 35.22813051420855 39.0 35.0 40.5 26.5 41.0 60-61 34.962862316671895 38.0 34.5 40.0 26.0 41.0 62-63 34.626405312634425 38.0 34.0 40.0 26.0 41.0 64-65 34.28639407571988 37.0 34.0 40.0 25.5 41.0 66-67 33.91852279266057 37.0 34.0 39.5 25.0 41.0 68-69 33.518457110979696 36.0 33.0 39.0 23.5 41.0 70-71 33.11547565747522 36.0 33.0 39.0 22.5 40.5 72-73 32.70692003993574 35.0 32.5 38.5 22.0 40.0 74-75 32.25678344917411 35.0 32.0 37.5 20.5 39.5 76-77 31.82175284693774 35.0 32.0 37.0 20.0 39.0 78-79 31.400060893222975 35.0 31.0 36.5 18.0 39.0 80-81 30.962148836444634 35.0 31.0 36.0 17.0 37.5 82-83 30.560710064460622 34.5 31.0 35.5 12.0 37.0 84-85 30.17548580900597 34.0 30.5 35.0 7.5 37.0 86-87 29.817189047519857 34.0 30.0 35.0 6.5 36.0 88-89 29.468821153491625 34.0 30.0 35.0 2.0 36.0 90-91 29.099910775067777 34.0 29.0 35.0 2.0 36.0 92-93 28.744605602655362 34.0 29.0 35.0 2.0 35.0 94-95 28.346498400256024 34.0 29.0 35.0 2.0 35.0 96-97 27.845958501627678 34.0 28.5 35.0 2.0 35.0 98-99 27.209263990078316 34.0 27.0 35.0 2.0 35.0 100-101 24.702578105733938 31.5 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2563058397829643 1101 2 0.2277307693174997 1101 3 0.21887717530595552 1101 4 0.279682945516015 1101 5 0.24977408288162195 1101 6 0.011317296433375645 1101 7 0.03559245053354232 1101 8 -0.03830822473761941 1101 9 0.12798030867661225 1101 10-11 0.13071155827964276 1101 12-13 0.2163592341880971 1101 14-15 0.2517837219709733 1101 16-17 0.36124391616437634 1101 18-19 0.44348561378768636 1101 20-21 0.32481183020656346 1101 22-23 0.26743684455853867 1101 24-25 0.32676693390836675 1101 26-27 0.19110251568200454 1101 28-29 0.1896291030766193 1101 30-31 0.26182746586912486 1101 32-33 0.3093756134824659 1101 34-35 0.40647791354487595 1101 36-37 0.418836351956017 1101 38-39 0.3391588326966968 1101 40-41 0.15824012461120418 1101 42-43 0.15434526947763771 1101 44-45 0.31904692919107447 1101 46-47 0.3804339624152533 1101 48-49 0.25925460404773304 1101 50-51 0.4396890947280596 1101 52-53 0.44474283321401487 1101 54-55 0.38094379134752643 1101 56-57 0.26105262812163943 1101 58-59 0.1681480602266774 1101 60-61 0.37312420344431985 1101 62-63 0.37103530379521743 1101 64-65 0.21470501804454756 1101 66-67 0.24146900261876425 1101 68-69 0.42718281047357465 1101 70-71 0.33197518451718366 1101 72-73 0.10787956140486443 1101 74-75 0.26794394386833886 1101 76-77 0.16486477089153695 1101 78-79 0.27363904461369515 1101 80-81 0.28313345901790754 1101 82-83 0.20470123591470468 1101 84-85 0.2284905054199271 1101 86-87 0.2759142669967787 1101 88-89 0.18040845298997965 1101 90-91 0.15441321443454115 1101 92-93 0.2515161287529608 1101 94-95 0.2947564722409304 1101 96-97 0.017101092756952596 1101 98-99 -0.2968904334772624 1101 100-101 -0.1668658267974088 1102 1 0.23676718855924506 1102 2 0.24427545980607235 1102 3 0.16916537567063727 1102 4 0.14265887902063668 1102 5 0.2523170063022562 1102 6 0.2202570342826462 1102 7 0.2802318809643296 1102 8 0.24201663622522318 1102 9 0.23549726447068053 1102 10-11 0.2627790277570696 1102 12-13 0.18720100887195912 1102 14-15 0.29144398569052754 1102 16-17 0.3497296287997713 1102 18-19 0.24393511793188338 1102 20-21 0.3348261065180864 1102 22-23 0.2979528169842993 1102 24-25 -0.037541431568065775 1102 26-27 0.15127948632286348 1102 28-29 0.10406696796223969 1102 30-31 0.14253237479524472 1102 32-33 0.33423204812697804 1102 34-35 0.28773030689505674 1102 36-37 0.13760103741007867 1102 38-39 0.26443407242392425 1102 40-41 0.2008353398362317 1102 42-43 0.1996928819748902 1102 44-45 0.13443575188028234 1102 46-47 0.1405769537232473 1102 48-49 0.2470598386477505 1102 50-51 0.23680983652577936 1102 52-53 0.3349197040205283 1102 54-55 0.6823563403822277 1102 56-57 0.6359942428566185 1102 58-59 0.5036634871290318 1102 60-61 0.49397367963489813 1102 62-63 0.537822841331149 1102 64-65 0.6681804299147132 1102 66-67 0.494538195664866 1102 68-69 0.4675688342355784 1102 70-71 0.3352085694078184 1102 72-73 0.31079483128205965 1102 74-75 0.30886639896605317 1102 76-77 0.31767549994611244 1102 78-79 0.5744356513868851 1102 80-81 0.47513219967893505 1102 82-83 0.5761536402466625 1102 84-85 0.49402312522292746 1102 86-87 0.29854011710406425 1102 88-89 0.4598836523556038 1102 90-91 0.28026296516957316 1102 92-93 0.34822637311178894 1102 94-95 0.39470812389322063 1102 96-97 0.22159124944695918 1102 98-99 0.1557037620112247 1102 100-101 0.2533604691415441 1103 1 0.16294903428946483 1103 2 0.2808623717434564 1103 3 0.2517950476457784 1103 4 0.24899272024247665 1103 5 0.22642783422236334 1103 6 0.1433954141878857 1103 7 0.13086047995737005 1103 8 0.22955558787222685 1103 9 0.2525389723984546 1103 10-11 0.26029176555441325 1103 12-13 0.34109760369984343 1103 14-15 0.4306392568388375 1103 16-17 0.5211588131401044 1103 18-19 0.5062431039954802 1103 20-21 0.5243881473795327 1103 22-23 0.6256155017123106 1103 24-25 0.3241055263962451 1103 26-27 0.4371965574836878 1103 28-29 0.3553227744729526 1103 30-31 0.5370761293513624 1103 32-33 0.6683766888982916 1103 34-35 0.450575247838529 1103 36-37 0.6652115804642591 1103 38-39 0.7351616826191076 1103 40-41 0.7314385212127235 1103 42-43 0.6276216090609665 1103 44-45 0.5961102687870934 1103 46-47 0.629121053056032 1103 48-49 0.6976535734526479 1103 50-51 0.7867386780220471 1103 52-53 0.7537959175996249 1103 54-55 0.7753250607892213 1103 56-57 0.9092535265096373 1103 58-59 0.9087159728065046 1103 60-61 0.9727809301491561 1103 62-63 1.0627605921349215 1103 64-65 0.9133775208035644 1103 66-67 0.6958050566742671 1103 68-69 0.8015039806612947 1103 70-71 0.8433258955178573 1103 72-73 0.7236129090206944 1103 74-75 0.6755046949962562 1103 76-77 0.6423293124726399 1103 78-79 0.6888686522349126 1103 80-81 0.6928739356681319 1103 82-83 0.7207605909752921 1103 84-85 0.58577662381715 1103 86-87 0.7560183695037779 1103 88-89 0.7799929450145697 1103 90-91 0.8077558125997086 1103 92-93 0.7810188863559269 1103 94-95 0.7975471974489281 1103 96-97 0.9273616591050384 1103 98-99 0.9951445126039538 1103 100-101 0.9993193927889727 1104 1 0.30329370838463277 1104 2 0.31143148762299333 1104 3 0.32631222642207547 1104 4 0.24110133050344018 1104 5 0.30346195741348936 1104 6 0.28272777698267504 1104 7 0.17900996025269933 1104 8 0.2517861556047265 1104 9 0.314015726264401 1104 10-11 0.37394812167315905 1104 12-13 0.4209117176304389 1104 14-15 0.41413772819993966 1104 16-17 0.5045824190352732 1104 18-19 0.43215986010739016 1104 20-21 0.5367106368841306 1104 22-23 0.4781732559436378 1104 24-25 0.4802132464091571 1104 26-27 0.43773505989769035 1104 28-29 0.5329311239254153 1104 30-31 0.32817238762419265 1104 32-33 0.36546165550585386 1104 34-35 0.5162861727443939 1104 36-37 0.42766691548314384 1104 38-39 0.5351113925561108 1104 40-41 0.6756360814122857 1104 42-43 0.6518395701000017 1104 44-45 0.3964623806035519 1104 46-47 0.5123018344399242 1104 48-49 0.6718249877019886 1104 50-51 0.5458500900417036 1104 52-53 0.7198912197102558 1104 54-55 0.5935211936624043 1104 56-57 0.66315263390171 1104 58-59 0.6989618570368279 1104 60-61 0.5750758414946162 1104 62-63 0.6902623803616734 1104 64-65 0.6350800021868821 1104 66-67 0.29693650171609676 1104 68-69 0.3056179193904285 1104 70-71 0.2809759824675808 1104 72-73 0.40503370095085245 1104 74-75 0.5031888050452338 1104 76-77 0.5519123769277314 1104 78-79 0.47578322666949546 1104 80-81 0.5524167599352872 1104 82-83 0.4665620217194437 1104 84-85 0.42338895253188014 1104 86-87 0.5612654214726192 1104 88-89 0.5664306589812895 1104 90-91 0.48075240866227276 1104 92-93 0.2697064703252785 1104 94-95 0.4909629424701727 1104 96-97 0.4115042175997843 1104 98-99 0.2943264446375018 1104 100-101 0.29670882585395475 1105 1 0.17812486612679734 1105 2 0.25940923721804054 1105 3 0.23993763967078507 1105 4 0.2886395072402479 1105 5 0.42238072119218373 1105 6 0.32435266285158804 1105 7 0.1780686590596332 1105 8 0.2812113474974254 1105 9 0.31261162238851625 1105 10-11 0.33126444013163336 1105 12-13 0.34299426691982404 1105 14-15 0.2131759775860118 1105 16-17 0.3729159526772605 1105 18-19 0.428924501623662 1105 20-21 0.37570911752803937 1105 22-23 0.14890551562258025 1105 24-25 0.13835251952193772 1105 26-27 0.09246654465121651 1105 28-29 0.02708510088933025 1105 30-31 -0.1160437448162952 1105 32-33 0.09919584532310921 1105 34-35 -0.10391561281293349 1105 36-37 0.04589820202079409 1105 38-39 0.04214872889347987 1105 40-41 0.023720428098137347 1105 42-43 0.09468366299777387 1105 44-45 -0.07752851222893042 1105 46-47 -0.13173518618708613 1105 48-49 0.05145009959081648 1105 50-51 -0.05765873460092763 1105 52-53 -0.07656618719554587 1105 54-55 0.042478811340750156 1105 56-57 0.2551505871658222 1105 58-59 0.2754935904122533 1105 60-61 0.17841627233162427 1105 62-63 0.08981782968388075 1105 64-65 0.212512676056285 1105 66-67 0.2116570352665974 1105 68-69 0.19508117590688556 1105 70-71 0.08809354690567517 1105 72-73 0.026713584999050966 1105 74-75 0.20016426394638387 1105 76-77 0.03479966474257168 1105 78-79 0.23591600440043337 1105 80-81 0.34505537202950265 1105 82-83 0.20011032809481932 1105 84-85 0.42967176802249796 1105 86-87 0.27531928596470934 1105 88-89 0.17864797712943314 1105 90-91 0.22023396202898127 1105 92-93 0.27333624252949207 1105 94-95 0.34668487877919674 1105 96-97 0.37236263585501916 1105 98-99 0.10889167918697851 1105 100-101 0.38988845069677325 1106 1 0.05304558227975775 1106 2 0.1870075244893563 1106 3 0.15588885812272935 1106 4 0.16656658783610823 1106 5 0.27073270393053406 1106 6 0.18505464845502217 1106 7 0.18100833615455514 1106 8 0.2670780755493851 1106 9 0.32033500524502756 1106 10-11 0.15092703178968492 1106 12-13 0.2832075775168832 1106 14-15 0.45888230033696686 1106 16-17 0.19281071510234682 1106 18-19 0.1909161217409121 1106 20-21 0.3855492062073864 1106 22-23 0.3043529827877052 1106 24-25 0.28853958988252515 1106 26-27 0.2966540655218921 1106 28-29 0.23167595672017427 1106 30-31 0.22780412504161518 1106 32-33 0.30283529498947814 1106 34-35 0.280105209447008 1106 36-37 0.19800907887544383 1106 38-39 0.1113788407503975 1106 40-41 0.2180481010752331 1106 42-43 0.131966089350243 1106 44-45 0.10881989606757259 1106 46-47 0.12601042578541666 1106 48-49 0.23353570441047822 1106 50-51 0.16787466538681883 1106 52-53 0.3364586916310728 1106 54-55 0.1725038265059453 1106 56-57 0.45167400282262093 1106 58-59 0.25434159858466643 1106 60-61 0.32784532983686887 1106 62-63 0.2578395667954396 1106 64-65 0.23234376441356375 1106 66-67 0.16445721230915922 1106 68-69 0.21692745712397254 1106 70-71 0.17428175737292406 1106 72-73 0.12254923180704935 1106 74-75 0.12194346263121503 1106 76-77 0.3373208910784484 1106 78-79 0.17099414221305054 1106 80-81 0.045536064313335345 1106 82-83 0.25741427223156776 1106 84-85 0.2804215314059064 1106 86-87 0.5787027550109123 1106 88-89 0.6604445540431634 1106 90-91 0.7594105560541919 1106 92-93 0.41630304902553306 1106 94-95 0.4274894443432302 1106 96-97 0.6812097746220047 1106 98-99 0.45125242118499287 1106 100-101 0.47785424947288746 1107 1 0.17151931630848694 1107 2 0.24593742556370657 1107 3 0.17665563042312016 1107 4 0.0507073288848261 1107 5 0.19617804752450496 1107 6 0.19230304032607393 1107 7 0.06860156659715244 1107 8 0.07544007613577719 1107 9 0.1573606591742731 1107 10-11 0.09276468055477238 1107 12-13 0.1478272286286355 1107 14-15 0.23789949096956065 1107 16-17 0.2536823039872118 1107 18-19 0.05072798968670611 1107 20-21 0.3434041595361421 1107 22-23 0.215536964187244 1107 24-25 0.3151033478151817 1107 26-27 0.10079791672683314 1107 28-29 0.29812719827185674 1107 30-31 0.2768279625532273 1107 32-33 -0.04074868489817618 1107 34-35 0.08033220765489091 1107 36-37 0.00659003230030919 1107 38-39 0.18015136036030555 1107 40-41 0.03278727181903207 1107 42-43 0.3701281922046249 1107 44-45 0.3749115798013989 1107 46-47 0.43616913554574666 1107 48-49 0.40454192094322394 1107 50-51 0.17209119489523772 1107 52-53 0.43191861948192667 1107 54-55 0.6024532270719263 1107 56-57 0.6173133100137278 1107 58-59 0.4836384063008765 1107 60-61 0.5470052420829461 1107 62-63 0.47265389964803006 1107 64-65 0.5834488147997163 1107 66-67 0.5040673736647108 1107 68-69 0.4321176005205274 1107 70-71 0.45917449747995676 1107 72-73 0.6131599077422649 1107 74-75 0.6977438014006552 1107 76-77 0.45972956668644827 1107 78-79 0.5282482306375975 1107 80-81 0.5188140987809895 1107 82-83 0.45910007409841924 1107 84-85 0.3631678857466518 1107 86-87 0.48674202289309676 1107 88-89 0.6024031533281509 1107 90-91 0.677164742481061 1107 92-93 0.4608328187821762 1107 94-95 0.44733315899287973 1107 96-97 0.2490141501150518 1107 98-99 0.05809924653082277 1107 100-101 0.3548554001227018 1108 1 -0.17096617532753555 1108 2 0.01590610682304927 1108 3 -0.10941236136921262 1108 4 0.02510496889551206 1108 5 0.08473278142889029 1108 6 0.046880066807744925 1108 7 -0.03522147967614586 1108 8 0.02722399387719321 1108 9 0.1492081238341143 1108 10-11 -0.03521017350898603 1108 12-13 0.07686129273258047 1108 14-15 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2316 52-53 -1.3603254538789784 2316 54-55 -1.4057200602359856 2316 56-57 -1.347397028807869 2316 58-59 -1.6570736631160514 2316 60-61 -1.50233869764579 2316 62-63 -1.4793629309485965 2316 64-65 -1.6277744920361954 2316 66-67 -1.2068142705741565 2316 68-69 -1.1926489764597008 2316 70-71 -1.3959240430884563 2316 72-73 -1.9636550664875863 2316 74-75 -1.9859632872935933 2316 76-77 -2.0958209340782687 2316 78-79 -2.255591376103798 2316 80-81 -2.4335490365675305 2316 82-83 -1.9057872236661062 2316 84-85 -1.8688213271233813 2316 86-87 -1.9765889769837308 2316 88-89 -1.8587927647941385 2316 90-91 -1.8404850397399812 2316 92-93 -2.215048834741001 2316 94-95 -1.7593485730136678 2316 96-97 -1.979435264942488 2316 98-99 -2.235475887364398 2316 100-101 -2.4760339934200033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2321.0 3 813.0 4 772.0 5 892.0 6 1020.0 7 1187.0 8 1459.0 9 1842.0 10 2197.0 11 2956.0 12 3762.0 13 4634.0 14 5462.0 15 6743.0 16 8739.0 17 12037.0 18 10688.0 19 6470.0 20 6226.0 21 6661.0 22 7158.0 23 8050.0 24 9204.0 25 10612.0 26 12431.0 27 14824.0 28 17584.0 29 21399.0 30 26514.0 31 33328.0 32 42246.0 33 56503.0 34 78888.0 35 114014.0 36 175659.0 37 264466.0 38 233785.0 39 39450.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.998678054345056 19.075532111065787 14.402661772700384 22.52312806188877 2 15.281498130688947 20.481886359935764 40.71131196582561 23.52530354354968 3 19.089899047947437 25.234980109699954 29.893237297984154 25.781883544368462 4 12.646429845785176 17.0017074667946 37.844217403069926 32.507645284350296 5 13.79699728477879 37.43587286375978 34.031225043922696 14.735904807538732 6 31.12405852044487 36.45519024414393 17.979554732968335 14.441196502442876 7 28.1482853222499 33.18020978591173 20.95935427893177 17.712150612906598 8 26.200902303669103 34.68463289016649 20.147722282109633 18.966742524054776 9 26.589246055125265 17.071412491078828 20.589986891663834 35.749354562132076 10-11 24.552857600163488 27.180310646718986 27.45240792319169 20.814423829925833 12-13 25.75094960269382 24.512464096734725 27.96145301687229 21.77513328369917 14-15 22.81874480140364 25.73391009481601 26.51495679078374 24.93238831299661 16-17 21.945922948223455 29.173757631101687 26.84539356049963 22.03492586017523 18-19 21.783040841380654 27.43886085386471 29.565899690133605 21.212198614621034 20-21 22.714951521716053 26.578128379850725 29.561416601946476 21.14550349648675 22-23 22.386024423337854 26.6164498363796 29.456341288211352 21.541184452071192 24-25 22.188130490857084 26.805119266523647 29.504418224537748 21.50233201808152 26-27 21.914346425115472 27.058429185662554 29.355094222922755 21.67213016629922 28-29 21.704042065929734 27.039419051537305 29.58649561387582 21.67004326865714 30-31 21.935646318114816 26.89126130375343 29.510428064194066 21.662664313937686 32-33 21.7398712616415 27.04630821947053 29.368682444983325 21.845138073904643 34-35 22.190888834264953 27.110714023489002 29.10834054262848 21.59005659961756 36-37 22.179179306200332 26.85598633054885 29.069490899136717 21.8953434641141 38-39 21.903279380627943 27.08096736580924 29.32615914784467 21.689594105718147 40-41 22.123730658605027 26.915424592900944 29.22525881635611 21.735585932137923 42-43 21.95395730763736 27.00439182098162 29.028276687116072 22.01337418426494 44-45 22.056257354792603 27.211764954695077 28.819454281870964 21.912523408641356 46-47 22.244062910759904 27.313284060101534 28.34224385541092 22.10040917372765 48-49 22.33025592895154 27.55043080469017 28.04208925111173 22.07722401524656 50-51 22.24180614391562 27.665656833294495 27.986004922538648 22.106532100251233 52-53 22.483647298811498 27.671060906429666 27.8425687388069 22.002723055951936 54-55 22.175409641121576 27.56253656262994 27.97080097399255 22.291252822255935 56-57 22.286343399631047 27.99296413953765 27.725168883396336 21.995523577434966 58-59 22.37869057932354 27.602175411181683 27.78784912584113 22.23128488365365 60-61 22.492607534927153 27.463496586097776 28.174130961359655 21.869764917615417 62-63 22.29009174956264 27.5962987319789 28.204929703362236 21.908679815096217 64-65 22.133049214083055 27.622775829113444 28.015993679144767 22.228181277658727 66-67 22.06140819978577 27.973392121398845 27.701175388829736 22.26402428998565 68-69 22.0228980062124 27.784515928709432 28.03758571187401 22.15500035320416 70-71 22.096033229590653 27.795294417254734 27.939153062165378 22.169519290989236 72-73 22.10607566958326 27.772254486138976 27.751221188473114 22.37044865580465 74-75 22.08343311461277 28.060331752797868 27.58856586362693 22.267669268962432 76-77 22.240318451047973 27.919553746997156 27.654537137054184 22.185590664900687 78-79 22.156552600289526 27.90110123006812 27.56212304991756 22.380223119724793 80-81 22.034741455270044 28.14226358040045 27.54567256922999 22.277322395099514 82-83 22.186565260556407 28.068580060302356 27.572580590432977 22.172274088708257 84-85 22.12403226392269 27.866967396419263 27.436667343854086 22.572332995803965 86-87 22.234616492885245 27.710587084008143 27.6624157200681 22.39238070303851 88-89 22.233960143659388 27.73772383921291 27.526840351541654 22.50147566558604 90-91 22.06580677865301 27.772799572118235 27.816705350318216 22.344688298910537 92-93 22.099597263033267 27.705660711769426 27.804179379021033 22.390562646176274 94-95 21.937088735708514 27.743358311170425 27.648454236920163 22.6710987162009 96-97 22.070684494112953 27.818878467371782 27.547914587906607 22.56252245060866 98-99 21.909175643121312 27.82315735690064 27.40470188590372 22.86296511407432 100-101 21.965505760351185 27.736276599057362 27.470715187382076 22.827502453209373 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 286.0 1 203.0 2 146.5 3 182.5 4 182.5 5 217.5 6 350.5 7 640.5 8 1084.5 9 1392.0 10 1336.5 11 1276.5 12 1364.0 13 1468.5 14 1588.0 15 1652.0 16 1755.5 17 1833.0 18 1882.5 19 2065.0 20 2338.5 21 2654.5 22 3153.0 23 3854.0 24 4646.5 25 5602.0 26 7119.5 27 9187.0 28 10805.0 29 12451.5 30 14800.5 31 17405.5 32 20163.0 33 23077.5 34 26715.5 35 29381.5 36 32321.0 37 35716.0 38 37016.0 39 37750.0 40 38593.5 41 40184.0 42 41651.5 43 43222.0 44 45179.0 45 46439.5 46 46290.5 47 46196.5 48 48678.5 49 49060.5 50 46996.5 51 45334.0 52 43268.0 53 41930.5 54 40283.0 55 36872.0 56 33279.0 57 29935.5 58 26987.5 59 23790.0 60 19826.5 61 15748.5 62 12168.0 63 9326.5 64 7014.5 65 5217.5 66 3670.0 67 2797.0 68 2028.5 69 1266.5 70 850.5 71 573.5 72 399.5 73 290.5 74 219.5 75 182.5 76 119.5 77 62.5 78 39.0 79 25.0 80 17.5 81 15.5 82 15.0 83 9.0 84 4.0 85 4.5 86 3.5 87 4.0 88 4.5 89 2.0 90 2.0 91 2.5 92 1.5 93 2.0 94 1.5 95 0.5 96 2.0 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025059596348960468 2 0.011572106594265184 3 0.011252876067526833 4 0.02234613687168449 5 0.06488360455956961 6 0.047565348484014135 7 0.016919217917132544 8 0.052912459806881494 9 0.029927861881720304 10-11 0.026815364246021392 12-13 0.041140834133404844 14-15 0.021627868186523205 16-17 0.01955286976272393 18-19 0.017158640812186307 20-21 0.014684604229964093 22-23 0.009018262380358385 24-25 0.001675960265376337 26-27 3.9903815842293734E-5 28-29 0.0021947098713261557 30-31 0.0 32-33 5.187496059498186E-4 34-35 7.980763168458747E-5 36-37 0.0 38-39 1.5961526336917493E-4 40-41 7.980763168458747E-5 42-43 1.5961526336917493E-4 44-45 2.7932671089605615E-4 46-47 0.0 48-49 7.182686851612872E-4 50-51 7.980763168458747E-5 52-53 7.182686851612872E-4 54-55 0.0020749984237992745 56-57 2.7932671089605615E-4 58-59 6.783648693189935E-4 60-61 0.003830766320860199 62-63 0.0029528823723297365 64-65 0.0014365373703225744 66-67 0.01205095238437271 68-69 0.015682199626021437 70-71 0.03148411069956976 72-73 0.018954312525089525 74-75 0.02178748344989238 76-77 0.03591343425806436 78-79 0.02214661779247302 80-81 0.051675441515770394 82-83 0.039225450972974744 84-85 0.02254565595089596 86-87 0.01548268054680997 88-89 0.015043738572544738 90-91 0.026376422271756163 92-93 0.036591799127383355 94-95 0.013487489754695282 96-97 0.020989407133046506 98-99 0.034357185440214905 100-101 0.045649965323584035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1253013.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.8059422268195 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9131718885163 52.07439883472509 2 10.264117272018652 12.89295112791407 3 2.8234108521785886 5.31980936593511 4 1.2137877140222793 3.0493232417002627 5 0.6626237706484304 2.08083551287313 6 0.39610767338528846 1.492674939014179 7 0.27919860509866745 1.227473202314485 8 0.20757269694154215 1.0429439049580487 9 0.16426061101926864 0.928488820123643 >10 0.9567160722932757 11.337089479117212 >50 0.08093218587494466 3.523312961289245 >100 0.037336100644510734 3.9052992833543807 >500 2.548524527511831E-4 0.09899864650153732 >1k 3.822786791267746E-4 0.5971931286939948 >5k 1.2742622637559154E-4 0.42920755148564643 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5363 0.42800832872444255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4031 0.32170456332057207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2397 0.19129889314795617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.980763168458747E-5 0.0 3 0.0 0.0 0.0 7.980763168458747E-5 0.0 4 0.0 0.0 0.0 7.980763168458747E-5 0.0 5 0.0 0.0 0.0 7.980763168458747E-5 0.0 6 0.0 0.0 0.0 7.980763168458747E-5 0.0 7 0.0 0.0 0.0 7.980763168458747E-5 0.0 8 0.0 0.0 0.0 7.980763168458747E-5 0.0 9 0.0 0.0 0.0 7.980763168458747E-5 0.0 10-11 0.0 0.0 0.0 7.980763168458747E-5 0.0 12-13 0.0 0.0 0.0 7.980763168458747E-5 7.980763168458747E-5 14-15 0.0 0.0 0.0 7.980763168458747E-5 7.980763168458747E-5 16-17 0.0 0.0 0.0 1.9951907921146865E-4 7.980763168458747E-5 18-19 0.0 0.0 0.0 3.1923052673834987E-4 7.980763168458747E-5 20-21 0.0 0.0 0.0 3.1923052673834987E-4 7.980763168458747E-5 22-23 0.0 0.0 0.0 4.7884579010752486E-4 7.980763168458747E-5 24-25 0.0 0.0 0.0 9.177877643727558E-4 7.980763168458747E-5 26-27 0.0 0.0 0.0 0.0013168259227956934 7.980763168458747E-5 28-29 0.0 0.0 0.0 0.0023543251346953304 7.980763168458747E-5 30-31 0.0 0.0 0.0 0.004309612110967724 7.980763168458747E-5 32-33 0.0 0.0 0.0 0.006384610534766997 7.980763168458747E-5 34-35 0.0 0.0 0.0 0.008260089879354802 1.5961526336917493E-4 36-37 0.0 0.0 0.0 0.01248989435863794 1.5961526336917493E-4 38-39 0.0 0.0 0.0 0.0178370056815053 1.5961526336917493E-4 40-41 0.0 0.0 0.0 0.02382257805784936 1.5961526336917493E-4 42-43 0.0 0.0 0.0 0.03220237938473104 1.5961526336917493E-4 44-45 0.0 0.0 0.0 0.03978410439476686 1.5961526336917493E-4 46-47 0.0 0.0 0.0 0.05323169033361984 1.5961526336917493E-4 48-49 0.0 0.0 0.0 0.0700311968032255 1.5961526336917493E-4 50-51 0.0 0.0 0.0 0.07717397983899608 1.5961526336917493E-4 52-53 0.0 0.0 0.0 0.09133983446301036 1.5961526336917493E-4 54-55 0.0 0.0 0.0 0.11995087042193496 1.5961526336917493E-4 56-57 0.0 0.0 0.0 0.16348593350587742 1.5961526336917493E-4 58-59 0.0 0.0 0.0 0.20546474777197044 1.5961526336917493E-4 60-61 0.0 0.0 0.0 0.2611305708719702 1.9951907921146865E-4 62-63 0.0 0.0 0.0 0.31188822462336785 2.394228950537624E-4 64-65 0.0 0.0 0.0 0.36188770587376184 2.394228950537624E-4 66-67 0.0 0.0 0.0 0.4486785053307507 2.394228950537624E-4 68-69 0.0 0.0 0.0 0.5599303438990657 2.394228950537624E-4 70-71 0.0 0.0 0.0 0.6124437655475243 3.1923052673834987E-4 72-73 0.0 0.0 0.0 0.684270634063653 3.1923052673834987E-4 74-75 0.0 0.0 0.0 0.7715402793107493 3.1923052673834987E-4 76-77 0.0 0.0 0.0 0.857213771924154 3.1923052673834987E-4 78-79 0.0 0.0 0.0 0.9469973575693149 3.1923052673834987E-4 80-81 0.0 0.0 0.0 1.1053356988315364 3.1923052673834987E-4 82-83 0.0 0.0 0.0 1.284184601436697 3.1923052673834987E-4 84-85 0.0 0.0 0.0 1.5373344091402084 3.1923052673834987E-4 86-87 0.0 0.0 0.0 1.898064904354544 3.1923052673834987E-4 88-89 0.0 0.0 0.0 2.27846798077913 3.1923052673834987E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3255 0.0 42.47186 1 GTATCAA 5690 0.0 40.410328 1 TATCACG 55 0.0025767917 34.55344 2 ATCAACG 7040 0.0 32.461334 3 TCAACGC 7135 0.0 32.029125 4 TATCAAC 7310 0.0 31.327349 2 CAACGCA 7330 0.0 31.24187 5 AACGCAG 7470 0.0 30.783554 6 ACGCAGA 8355 0.0 27.067894 7 CGCAGAG 8325 0.0 26.994228 8 GCAGAGT 8890 0.0 24.636318 9 GTACATG 5920 0.0 24.074589 1 GAGTACT 4550 0.0 23.492096 12-13 TACATGG 6190 0.0 22.335533 2 ACATGGG 6090 0.0 21.84413 3 GTACTTT 4950 0.0 20.823889 14-15 AGTACTT 4800 0.0 20.78398 12-13 AGAGTAC 8110 0.0 20.532185 10-11 TGTACCG 190 8.235758E-6 20.004622 5 CAGAGTA 8705 0.0 19.947412 10-11 >>END_MODULE