FastQCFastQC Report
Thu 26 May 2016
SRR936813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253013
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71650.5718216810200693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.4652784927211449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37740.30119400197763313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.12330279095268763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35700.049.8939551
GTATCAA63350.041.7631651
ATCAACG81850.032.3778763
TATCAAC82350.032.181292
TCAACGC83050.031.9100464
CAACGCA84350.031.4257745
AACGCAG86950.030.4314356
ACGCAGA96450.027.089277
CGCAGAG96700.026.7736078
GCAGAGT102600.024.9098859
TGGTATC19000.024.4965722
GTACATG54650.024.3361971
GTGGTAT19350.024.3018341
GAGTACT62550.024.0323712-13
TACATGG57450.022.3206372
GTACTTT67200.022.263414-15
ACATGGG56350.022.2506583
AGTACTT65900.021.44133212-13
CAGAGTA101300.020.70418410-11
AGAGTAC94600.020.68916310-11
Produced by FastQC (version 0.11.5)