##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936813_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1253013 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2979282736891 33.0 31.0 34.0 31.0 34.0 2 32.42023107501678 34.0 31.0 34.0 31.0 34.0 3 32.522303439788736 34.0 31.0 34.0 31.0 34.0 4 36.14913093479477 37.0 35.0 37.0 35.0 37.0 5 36.06827622698248 37.0 35.0 37.0 35.0 37.0 6 36.11243618382252 37.0 35.0 37.0 35.0 37.0 7 36.06827064044826 37.0 35.0 37.0 35.0 37.0 8 36.03792777888178 37.0 35.0 37.0 35.0 37.0 9 37.74076166807527 39.0 37.0 39.0 35.0 39.0 10-11 37.65987982566821 39.0 37.0 39.0 35.0 39.0 12-13 37.56838476536157 39.0 37.0 39.0 35.0 39.0 14-15 38.80445055238852 40.0 38.0 41.0 35.0 41.0 16-17 38.71674036901453 40.0 38.0 41.0 35.0 41.0 18-19 38.87615930560976 40.0 38.0 41.0 35.0 41.0 20-21 38.910575149659266 40.0 38.0 41.0 35.0 41.0 22-23 38.82912108653302 40.0 38.0 41.0 35.0 41.0 24-25 38.724304935383756 40.0 38.0 41.0 34.5 41.0 26-27 38.553395295978575 40.0 38.0 41.0 34.0 41.0 28-29 38.47188696366279 40.0 38.0 41.0 34.0 41.0 30-31 38.455537173197726 40.0 38.0 41.0 34.0 41.0 32-33 38.28462633667807 40.0 38.0 41.0 34.0 41.0 34-35 38.125521044075356 40.0 38.0 41.0 34.0 41.0 36-37 37.96867869686906 40.0 37.0 41.0 33.0 41.0 38-39 37.80957260619004 40.0 37.0 41.0 33.0 41.0 40-41 37.637259549581685 40.0 37.0 41.0 33.0 41.0 42-43 37.380622946449876 40.0 37.0 41.0 33.0 41.0 44-45 37.083325153051085 40.0 36.5 41.0 31.5 41.0 46-47 36.67090165864201 40.0 36.0 41.0 31.0 41.0 48-49 36.26672388873858 39.0 36.0 41.0 30.0 41.0 50-51 35.967077755777474 39.0 35.5 40.5 29.0 41.0 52-53 35.71921121329149 39.0 35.0 40.0 28.5 41.0 54-55 35.42510811938902 39.0 35.0 40.0 28.0 41.0 56-57 35.15208062486183 38.0 35.0 40.0 27.0 41.0 58-59 34.813070574686776 38.0 34.0 40.0 26.0 41.0 60-61 34.486366063241164 38.0 34.0 40.0 26.0 41.0 62-63 34.12604697636816 37.0 34.0 40.0 26.0 41.0 64-65 33.73466915347247 36.5 33.0 39.0 25.0 40.5 66-67 33.35133913215585 36.0 33.0 39.0 23.5 40.0 68-69 33.03744095232851 36.0 32.5 39.0 23.0 40.0 70-71 32.580003559420376 35.0 32.0 38.0 22.0 40.0 72-73 32.06599612294525 35.0 31.5 37.5 20.5 39.5 74-75 31.55836092682199 35.0 31.0 37.0 19.0 39.0 76-77 30.16619739779236 33.5 29.5 35.5 18.0 37.5 78-79 30.90299980925976 34.0 31.0 36.0 18.0 38.0 80-81 30.645802956553524 34.0 31.0 36.0 15.0 37.0 82-83 30.334486154573018 34.0 30.5 35.0 13.0 37.0 84-85 29.990221968966004 34.0 30.0 35.0 9.5 36.5 86-87 29.64803278178279 34.0 30.0 35.0 7.0 36.0 88-89 29.26854190658836 34.0 29.0 35.0 2.0 36.0 90-91 28.83617328790683 34.0 29.0 35.0 2.0 35.0 92-93 28.368192109738686 34.0 29.0 35.0 2.0 35.0 94-95 27.792162571338046 33.0 27.5 35.0 2.0 35.0 96-97 27.148464142032047 33.0 26.5 34.5 2.0 35.0 98-99 26.344222685638538 33.0 25.0 34.0 2.0 35.0 100-101 22.804004427727406 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06601738166538951 1101 2 0.07858183309513578 1101 3 0.06945599203350383 1101 4 0.08243513820432469 1101 5 0.12093664531058579 1101 6 0.12010629069759204 1101 7 0.0970394125493712 1101 8 0.12228128583937803 1101 9 0.13724966830575624 1101 10-11 0.17374286860763988 1101 12-13 0.1912276473311323 1101 14-15 0.20954684665030499 1101 16-17 0.2239730102602877 1101 18-19 0.07189896479511759 1101 20-21 0.11518315723342454 1101 22-23 0.27522718856151585 1101 24-25 0.18963080184386172 1101 26-27 0.12076691744802304 1101 28-29 0.19805638078292986 1101 30-31 0.1692578806525873 1101 32-33 0.26191485505023593 1101 34-35 0.2810136841124802 1101 36-37 0.13296783501245812 1101 38-39 0.14378567083759464 1101 40-41 0.13731888320683794 1101 42-43 0.3007552987637041 1101 44-45 0.44051339793310973 1101 46-47 0.24241429659192448 1101 48-49 0.18016516039706687 1101 50-51 0.03990083756276874 1101 52-53 -0.12232951199602127 1101 54-55 -0.10625343398942988 1101 56-57 -0.08442750225714946 1101 58-59 -0.12382579175356767 1101 60-61 0.09604575656906889 1101 62-63 -8.181877090933654E-4 1101 64-65 0.01492122119022099 1101 66-67 -0.1977058725176093 1101 68-69 -0.10600866263991549 1101 70-71 -0.019445992130549428 1101 72-73 0.08933813040600569 1101 74-75 0.10412312599257234 1101 76-77 -0.09255481476549932 1101 78-79 -0.004743233526028234 1101 80-81 0.15584431151806655 1101 82-83 0.08651280402488126 1101 84-85 0.028775156195457896 1101 86-87 -0.5020645166815711 1101 88-89 -0.15996115210242934 1101 90-91 0.018658154569759944 1101 92-93 0.060653866769698084 1101 94-95 -0.10309720377872011 1101 96-97 -0.06424688924336763 1101 98-99 0.04521956838602037 1101 100-101 0.3705787931982414 1102 1 0.20365186387811463 1102 2 0.30027719143982523 1102 3 0.2264740256819593 1102 4 0.14511613252589228 1102 5 0.16359942699497765 1102 6 0.11059100784193276 1102 7 0.1415726620218578 1102 8 0.24134657964116712 1102 9 0.24753702720951765 1102 10-11 0.32932614007933125 1102 12-13 0.2605609963527229 1102 14-15 0.4391250180131081 1102 16-17 0.4057642238394763 1102 18-19 0.2862759307295022 1102 20-21 0.352814216554286 1102 22-23 0.24921214067312292 1102 24-25 0.30712459606959186 1102 26-27 0.16832675209381875 1102 28-29 0.3543527547281684 1102 30-31 0.44019439100006963 1102 32-33 0.4084148054896133 1102 34-35 0.43122426327291663 1102 36-37 0.3854394481857426 1102 38-39 0.43937912541260715 1102 40-41 0.4126429347983773 1102 42-43 0.40657988411244617 1102 44-45 0.5145555408630926 1102 46-47 0.7113110842792167 1102 48-49 0.6583991335972428 1102 50-51 0.7627031092220093 1102 52-53 0.7432128773108673 1102 54-55 0.7439210621858479 1102 56-57 0.7348810664534895 1102 58-59 0.8578251902241973 1102 60-61 0.925197635273193 1102 62-63 0.8099994698491173 1102 64-65 0.8046962944080178 1102 66-67 0.7324261298812687 1102 68-69 0.6363278033525859 1102 70-71 0.6862058084888574 1102 72-73 0.7678941763383591 1102 74-75 0.5470638750211485 1102 76-77 0.5612651325647526 1102 78-79 0.6988917350225847 1102 80-81 0.8707336415796831 1102 82-83 0.8060137677828756 1102 84-85 0.7625725766185703 1102 86-87 0.5694887203401429 1102 88-89 0.5708050479812883 1102 90-91 0.804273344508168 1102 92-93 0.8477334126375204 1102 94-95 0.7714867900657794 1102 96-97 0.7096668239311263 1102 98-99 0.7136682781340618 1102 100-101 0.9958471871417167 1103 1 0.32857388327992254 1103 2 0.3281043794768479 1103 3 0.3176148885976744 1103 4 0.1764734162423096 1103 5 0.2469449357376945 1103 6 0.22441175953238002 1103 7 0.06404120115053047 1103 8 0.17714158749140552 1103 9 0.37025549077335285 1103 10-11 0.25933145087694953 1103 12-13 0.2844949522939686 1103 14-15 0.38488472877176605 1103 16-17 0.48258796488281774 1103 18-19 0.39013642450225916 1103 20-21 0.44698347097859426 1103 22-23 0.3418652667384663 1103 24-25 0.3734067517122952 1103 26-27 0.41269862079111164 1103 28-29 0.3822636245471429 1103 30-31 0.4634380205553086 1103 32-33 0.5514557666589468 1103 34-35 0.5427083026640389 1103 36-37 0.357516209696918 1103 38-39 0.3137323792617792 1103 40-41 0.40866969286951615 1103 42-43 0.5043461023375215 1103 44-45 0.44847225024032156 1103 46-47 0.45887097763036166 1103 48-49 0.5617221313608383 1103 50-51 0.6129917630546302 1103 52-53 0.7249004818164693 1103 54-55 0.7080068899320224 1103 56-57 0.7567368925994558 1103 58-59 0.6882470546311055 1103 60-61 0.6234917637883086 1103 62-63 0.6438153745670689 1103 64-65 0.6567615917083316 1103 66-67 0.6675348698152916 1103 68-69 0.6141355107345987 1103 70-71 0.46196221062832166 1103 72-73 0.4415790968426023 1103 74-75 0.6208628965047573 1103 76-77 0.5269009092515091 1103 78-79 0.7906324784260264 1103 80-81 0.7324490137490933 1103 82-83 0.822680743666286 1103 84-85 0.8774893084140132 1103 86-87 0.7022468718733528 1103 88-89 0.4242162111696537 1103 90-91 0.3686841590546521 1103 92-93 0.43347960289394294 1103 94-95 0.32043058296477156 1103 96-97 0.5288185109790398 1103 98-99 0.6753205700872371 1103 100-101 0.7462311956884697 1104 1 0.13279824857131928 1104 2 0.1341377222768685 1104 3 0.15207350955951426 1104 4 -0.007508059437405734 1104 5 0.011170798338405064 1104 6 -0.12235165176139873 1104 7 0.08529160090770205 1104 8 0.02183561965588865 1104 9 0.07745706034411626 1104 10-11 0.13074024805744955 1104 12-13 0.05174940780639048 1104 14-15 -0.08830427967583176 1104 16-17 0.12015163104468485 1104 18-19 0.10940394729350089 1104 20-21 0.07105792631198682 1104 22-23 -0.022955166000201643 1104 24-25 0.0909137154212658 1104 26-27 -0.017529409653093353 1104 28-29 0.017677553267084534 1104 30-31 0.08273430958809058 1104 32-33 0.03210345058997177 1104 34-35 0.11884790560038994 1104 36-37 0.08979425189988177 1104 38-39 0.08420137071816214 1104 40-41 -0.04739668838612232 1104 42-43 0.06626391144848753 1104 44-45 -0.02515822032982129 1104 46-47 0.06643902179447281 1104 48-49 0.11918951402346067 1104 50-51 0.02110152396526388 1104 52-53 -0.010230992520547488 1104 54-55 -0.1857164951914001 1104 56-57 -0.09274161246738544 1104 58-59 -0.029992492074775612 1104 60-61 -0.012562386728703245 1104 62-63 -0.061631128768105725 1104 64-65 -0.10096643670149064 1104 66-67 -0.02466736296585026 1104 68-69 -0.2395576352506268 1104 70-71 -0.22813911001476583 1104 72-73 -0.1803846812919616 1104 74-75 -0.04503429693540184 1104 76-77 -0.24712317464338085 1104 78-79 -0.07399977345394859 1104 80-81 0.25448895935770466 1104 82-83 0.1940677728988085 1104 84-85 0.19937927896421925 1104 86-87 0.28565534192432196 1104 88-89 0.14803587979231736 1104 90-91 0.25286016288255553 1104 92-93 0.21780073993761917 1104 94-95 0.16602320415934813 1104 96-97 0.18290079463615427 1104 98-99 -0.08830575846962674 1104 100-101 0.40067702442069475 1105 1 0.16291540222719192 1105 2 0.1377863294739896 1105 3 0.09862249040701698 1105 4 0.08468923083495383 1105 5 0.08430259126828332 1105 6 0.10341264534356753 1105 7 0.1373210265480509 1105 8 0.2125125672390027 1105 9 0.18292494847502638 1105 10-11 0.09931159590747995 1105 12-13 0.11704337720043156 1105 14-15 0.14990815393082357 1105 16-17 0.13309082271854322 1105 18-19 0.04201010041661846 1105 20-21 0.09358480205305142 1105 22-23 0.0642640804924568 1105 24-25 0.06138986404191371 1105 26-27 0.24895659056574715 1105 28-29 0.0909104721707763 1105 30-31 0.20451128354019232 1105 32-33 0.05425676563841364 1105 34-35 0.13239542269987226 1105 36-37 0.12975874345373484 1105 38-39 0.20380379989411068 1105 40-41 0.26789391761631265 1105 42-43 0.20582941506928876 1105 44-45 0.19709469595490958 1105 46-47 0.04105438433221309 1105 48-49 0.1920096326659788 1105 50-51 0.1369149804017198 1105 52-53 0.23820996336431222 1105 54-55 0.19796867879908575 1105 56-57 0.09885578328093203 1105 58-59 0.14729681162244646 1105 60-61 0.2671047451323858 1105 62-63 0.4826672049628584 1105 64-65 0.08676440407465691 1105 66-67 -0.06766989403013923 1105 68-69 0.207274094716567 1105 70-71 -0.14732263997944273 1105 72-73 -0.11135106890176516 1105 74-75 0.060372601694663075 1105 76-77 0.0063487423821939615 1105 78-79 0.1286140751091196 1105 80-81 0.228525594315812 1105 82-83 0.21818575708817534 1105 84-85 0.27727139212485596 1105 86-87 -0.07417800426464183 1105 88-89 -0.2754348533194211 1105 90-91 -0.23431791569703364 1105 92-93 -0.13179802986827127 1105 94-95 -0.20458263684102462 1105 96-97 -0.25076101499408665 1105 98-99 -0.2997727263765917 1105 100-101 -0.2485739258495414 1106 1 0.09689097588657347 1106 2 0.14744463338255542 1106 3 0.15374773418632515 1106 4 0.10737182079371621 1106 5 0.10559907234982546 1106 6 0.07755277373894387 1106 7 0.056656010434409154 1106 8 0.12064160676335689 1106 9 0.10434156557899854 1106 10-11 0.06306949218345892 1106 12-13 0.1404498953700113 1106 14-15 0.16794732854892658 1106 16-17 0.12742832590471664 1106 18-19 -0.12096735629588551 1106 20-21 0.05053614301212406 1106 22-23 0.019400043645475762 1106 24-25 0.03950900419994241 1106 26-27 0.043060144655612476 1106 28-29 0.04914863195564578 1106 30-31 0.10647421327840334 1106 32-33 -0.03724988432512788 1106 34-35 -0.1431949826208836 1106 36-37 -0.17383096239084495 1106 38-39 -0.00843905863190031 1106 40-41 0.012479398940890007 1106 42-43 -0.16255347108009488 1106 44-45 -0.3098783307417605 1106 46-47 -0.06672867135122829 1106 48-49 0.010528659801309459 1106 50-51 0.056136595796509425 1106 52-53 -7.671119953513994E-4 1106 54-55 -0.20211119782437947 1106 56-57 -0.18748067069096663 1106 58-59 -0.03721571868324958 1106 60-61 0.020641397486798496 1106 62-63 0.07811483020169163 1106 64-65 -0.18328749350148144 1106 66-67 -0.10301171855645919 1106 68-69 0.0033202265539955533 1106 70-71 0.05952175423268358 1106 72-73 -0.05304335668286342 1106 74-75 0.014546044125207658 1106 76-77 -0.004064857489762375 1106 78-79 0.21471040523026375 1106 80-81 0.08795039371038982 1106 82-83 -0.028468760954947214 1106 84-85 0.0800314579625585 1106 86-87 -0.04636913316782909 1106 88-89 -0.008971970015366537 1106 90-91 -0.03375472869385732 1106 92-93 0.01813859245213223 1106 94-95 0.2606653511769892 1106 96-97 0.2880327442556947 1106 98-99 0.3796066070660764 1106 100-101 0.27373002816681336 1107 1 0.026879523384224058 1107 2 -0.012582623681034022 1107 3 -0.010790329320258252 1107 4 0.04826157385486596 1107 5 -0.04647136836902632 1107 6 -0.07130599871047139 1107 7 0.003396204937963887 1107 8 0.03419924211589631 1107 9 -0.030590078478745397 1107 10-11 0.08130953252910444 1107 12-13 0.012838457554508409 1107 14-15 -0.017187960547111913 1107 16-17 0.07359319550223375 1107 18-19 0.06778051964560206 1107 20-21 0.02402719078379789 1107 22-23 0.029293418202776422 1107 24-25 -0.0026727595934090687 1107 26-27 -0.013288758717337146 1107 28-29 0.10021215959634588 1107 30-31 0.038748401627522355 1107 32-33 -0.03743346993568508 1107 34-35 -0.04040566924727074 1107 36-37 0.06677066925326614 1107 38-39 0.02139846270318202 1107 40-41 0.049678973238258095 1107 42-43 0.05802598990175056 1107 44-45 -0.07482692407101865 1107 46-47 -0.11750062184574972 1107 48-49 -0.21888315890033994 1107 50-51 -0.09483746085692957 1107 52-53 0.029556911207706094 1107 54-55 0.17014426479246225 1107 56-57 0.13077956407182967 1107 58-59 0.10674239787957873 1107 60-61 -0.05861764018983706 1107 62-63 0.10618561001397353 1107 64-65 0.22566025317413363 1107 66-67 0.1690753852563418 1107 68-69 0.3501744601191632 1107 70-71 0.222357871137838 1107 72-73 0.2301474368227403 1107 74-75 0.26651972442814653 1107 76-77 0.16981771359431264 1107 78-79 0.42735707798405187 1107 80-81 0.4106129034228765 1107 82-83 0.3854276978406119 1107 84-85 0.30913253102045246 1107 86-87 0.12409982547519505 1107 88-89 0.22665287170953974 1107 90-91 0.31677580672278083 1107 92-93 0.32816771236971576 1107 94-95 0.32410227899058697 1107 96-97 0.54145810077625 1107 98-99 0.3397774388762791 1107 100-101 0.5375425505413141 1108 1 0.03343144940151177 1108 2 -0.025397729431517746 1108 3 0.05384848204543147 1108 4 0.07951835910834859 1108 5 0.04839144872611456 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-0.363405434912508 2316 62-63 -0.5071111307600589 2316 64-65 -0.46161572416967545 2316 66-67 -0.6267790466707837 2316 68-69 -0.7857431917674944 2316 70-71 -0.6340395443579183 2316 72-73 -0.25108433111408246 2316 74-75 -0.39825263358226337 2316 76-77 -0.1468496648694284 2316 78-79 -0.35500260979080167 2316 80-81 -0.5081729069333392 2316 82-83 -0.23434577738173346 2316 84-85 -0.5825675436872935 2316 86-87 -0.5342372680035687 2316 88-89 -0.803278504862682 2316 90-91 -0.6751558186689408 2316 92-93 -0.48262424543743165 2316 94-95 -0.6645572461850549 2316 96-97 -0.3291362621622689 2316 98-99 -0.48800078248518375 2316 100-101 -0.4129135875943639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 25.0 6 43.0 7 131.0 8 307.0 9 560.0 10 1054.0 11 1697.0 12 2671.0 13 4023.0 14 5214.0 15 7015.0 16 9970.0 17 14400.0 18 13232.0 19 7485.0 20 6031.0 21 6214.0 22 6354.0 23 6921.0 24 7852.0 25 9407.0 26 11593.0 27 14175.0 28 18027.0 29 22487.0 30 27877.0 31 35645.0 32 45810.0 33 60667.0 34 84160.0 35 123020.0 36 191848.0 37 291720.0 38 202531.0 39 12839.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11124416237576 19.352333053925676 15.139025266435159 22.3973975172634 2 15.592926258527726 20.841008418384448 40.1141446097758 23.45192071331203 3 19.332742222920984 25.100617792849057 30.35876039198441 25.207879592245547 4 12.909674814007275 17.4740583843972 37.37444194201783 32.24182485957769 5 13.666509179481123 37.485115864812144 34.00222823085493 14.846146724851797 6 31.185310926542662 36.549900120748944 17.61210777541813 14.652681177290258 7 28.00234315206626 33.33365256386007 20.76123711406027 17.9027671700134 8 26.405552057321035 34.5472872188876 19.860368567604645 19.186792156186726 9 26.519756778261677 16.682827712082794 20.587017054092815 36.21039845556271 10-11 24.573737335038224 27.17217962655224 27.241859863704498 21.012223174705035 12-13 25.872357270036307 24.428557405230432 28.072533964132855 21.62655136060041 14-15 22.866402822636317 25.483374873205626 26.868994974513434 24.781227329644626 16-17 21.661786429989156 28.865183362024176 27.37046622820354 22.10256397978313 18-19 21.476327954940412 26.80885788792166 30.64834009373448 21.06647406340345 20-21 22.22955131957671 25.996800469913524 30.826861846780893 20.94678636372887 22-23 21.905252925430453 26.05887733064747 30.7242824694448 21.311587274477287 24-25 21.92971778964823 26.128053221484144 30.78134135220722 21.16088763666041 26-27 21.745104212932866 26.345166705974233 30.563766247292868 21.345962833800037 28-29 21.40963984268257 26.343886072555005 30.80678654884885 21.439687535913578 30-31 21.67697273069728 26.142712117632787 30.807256916289433 21.373058235380498 32-33 21.558467160246312 26.27411747663824 30.656276300315533 21.511139062799916 34-35 21.893507888585354 26.28552137926741 30.45738551794754 21.36358521419969 36-37 21.856733460319894 26.06999744989279 30.403224229450586 21.670044860336734 38-39 21.61685073259196 26.310970839998337 30.656235732097077 21.415942695312626 40-41 21.80964812065119 26.14061207477571 30.535941525961086 21.513798278612022 42-43 21.718370040853525 26.217964219046408 30.284961129692988 21.778704610407075 44-45 21.86697185105023 26.439111166444402 29.920918617763743 21.77299836474163 46-47 22.030554485906908 26.722545555393722 29.366224862979674 21.8806750957197 48-49 22.230030311881972 27.005907544769485 28.92402396218916 21.840038181159386 50-51 22.17901223975408 27.047817288418187 28.68219219738125 22.090978274446485 52-53 22.384963284499044 27.19321347823207 28.511835072740666 21.909988164528222 54-55 22.123572800160897 27.09373189313779 28.587493156304422 22.195202150396895 56-57 22.226061972505438 27.57728605917915 28.362577066581533 21.834074901733874 58-59 22.36511847100615 27.078140824432744 28.512290833171722 22.044449871389386 60-61 22.301557404655455 26.910043400463852 29.01338566715244 21.77501352772825 62-63 22.060674456821467 27.026080071894388 29.0569288933548 21.85631657792935 64-65 22.08780838179986 26.9469378797099 28.79109346474995 22.174160273740288 66-67 21.980045275826996 27.445786526830098 28.42484735470015 22.14932084264276 68-69 22.026758860994626 27.07394341046388 28.845444014411747 22.053853714129744 70-71 22.04374447328181 27.195803046119565 28.700945886844735 22.05950659375389 72-73 22.1594269173584 27.049479933568126 28.467222606628983 22.32387054244449 74-75 22.096937541749366 27.295087920077442 28.247073254627047 22.360901283546138 76-77 22.10220484544055 27.38962005980784 28.315069356822313 22.193105737929294 78-79 22.09840712823781 27.346220655656456 28.231264692373475 22.324107523732263 80-81 22.062740650615112 27.456985666610933 28.14677926724494 22.333494415529014 82-83 22.187315923817003 27.412325860937774 28.169981434744646 22.230376780500574 84-85 22.020116240647834 27.221294609862735 28.147332866157242 22.61125628333219 86-87 22.075880164011295 27.1400780151042 28.273551682512444 22.51049013837206 88-89 22.066965094480416 27.067599566165022 28.11216342075209 22.75327191860247 90-91 21.898120714044865 27.133345703619906 28.441563364797755 22.526970217537475 92-93 21.85515900331083 26.91845266811837 28.43384542084112 22.792542907729676 94-95 21.700253708461126 27.061091943978234 28.35708807490425 22.88156627265639 96-97 21.863952609074747 26.926074386513775 28.31320469360107 22.896768310810405 98-99 21.697109193652462 26.87923562009099 28.188703895762895 23.234951290493655 100-101 21.41462211673368 26.98458746406641 28.221812186054972 23.378978233144935 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 400.0 1 298.5 2 224.5 3 257.0 4 226.0 5 214.5 6 373.0 7 848.5 8 1468.0 9 1782.0 10 1660.0 11 1497.0 12 1578.0 13 1779.5 14 1956.0 15 2079.0 16 2222.0 17 2382.5 18 2506.5 19 2594.5 20 2842.0 21 3191.5 22 3602.5 23 4264.0 24 5008.0 25 6108.5 26 7624.5 27 9308.5 28 10885.5 29 12701.5 30 15056.0 31 17474.0 32 20054.0 33 22652.5 34 26132.0 35 28976.5 36 31779.5 37 35093.0 38 36693.0 39 37280.0 40 38292.5 41 39770.5 42 41050.0 43 42700.5 44 44439.0 45 45781.0 46 46092.5 47 46334.5 48 48521.0 49 48562.0 50 46415.5 51 45162.5 52 43129.5 53 41839.5 54 40211.5 55 36667.5 56 32808.0 57 29403.0 58 26730.5 59 23920.0 60 20281.0 61 15963.0 62 12167.5 63 9363.5 64 6929.5 65 5042.0 66 3673.0 67 2868.5 68 2076.0 69 1209.0 70 814.5 71 596.0 72 397.0 73 277.5 74 224.0 75 167.0 76 109.5 77 60.0 78 30.0 79 18.0 80 10.0 81 10.5 82 10.0 83 5.5 84 1.5 85 1.5 86 1.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028970170301505255 2 0.0032721128990680862 3 3.1923052673834987E-4 4 5.586534217921123E-4 5 7.980763168458747E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011731721857634358 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.389419742652311E-4 20-21 0.0013168259227956931 22-23 0.0027932671089605614 24-25 0.010813934093261602 26-27 0.005865860928817179 28-29 3.990381584229373E-4 30-31 0.0023144213188530365 32-33 0.004588938821863779 34-35 0.0 36-37 0.00993605014473114 38-39 0.016041333968602082 40-41 9.975953960573434E-4 42-43 0.0 44-45 0.0 46-47 4.389419742652311E-4 48-49 0.0031124976356989116 50-51 0.0069831677724014034 52-53 0.0 54-55 0.0027932671089605614 56-57 0.003032690004014324 58-59 4.7884579010752486E-4 60-61 0.0024740365822222116 62-63 0.005107688427813598 64-65 3.9903815842293734E-5 66-67 0.002114902239641568 68-69 5.985572376344061E-4 70-71 7.182686851612872E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0019552869762723933 80-81 0.0023543251346953304 82-83 0.010774030277419308 84-85 0.00758172501003581 86-87 0.004030285400071667 88-89 1.5961526336917493E-4 90-91 8.379801326881685E-4 92-93 3.591343425806436E-4 94-95 0.0 96-97 0.0024341327663799177 98-99 0.0018754793445878055 100-101 5.586534217921123E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1253013.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.189617483408 #Duplication Level Percentage of deduplicated Percentage of total 1 81.60819656111697 49.11966134524401 2 10.921537726886918 13.147263562238656 3 3.080582756221473 5.562572931688595 4 1.3284566975914522 3.198372018852037 5 0.7399899217109396 2.226985516467924 6 0.4518948193621558 1.6319625792085148 7 0.29233091278565604 1.2316700073400897 8 0.221642644783753 1.0672468806035975 9 0.17586842100630243 0.9526907689002267 >10 1.0454128400337646 11.795096217239111 >50 0.08762434890500757 3.6237141036153684 >100 0.04500210873949077 4.628527038062465 >500 9.2924418124667E-4 0.35092614454900484 >1k 2.6549833749904854E-4 0.4249945725993071 >5k 2.6549833749904854E-4 1.0383163133912383 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7165 0.5718216810200693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5830 0.4652784927211449 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3774 0.30119400197763313 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1545 0.12330279095268763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5961526336917493E-4 0.0 10-11 0.0 0.0 0.0 1.5961526336917493E-4 0.0 12-13 0.0 0.0 0.0 1.5961526336917493E-4 0.0 14-15 0.0 0.0 0.0 1.9951907921146865E-4 0.0 16-17 0.0 0.0 0.0 5.187496059498185E-4 0.0 18-19 0.0 0.0 0.0 7.980763168458747E-4 0.0 20-21 0.0 0.0 0.0 7.980763168458747E-4 0.0 22-23 0.0 0.0 0.0 8.379801326881685E-4 0.0 24-25 0.0 0.0 0.0 0.0012370182911111058 0.0 26-27 0.0 0.0 0.0 0.0016360564495340431 0.0 28-29 0.0 0.0 0.0 0.0027134594772759736 0.0 30-31 0.0 0.0 0.0 0.0048283617169175425 0.0 32-33 0.0 0.0 0.0 0.0069432639565591096 0.0 34-35 0.0 0.0 0.0 0.008818743301146915 0.0 36-37 0.0 0.0 0.0 0.01324806685964152 0.0 38-39 0.0 0.0 0.0 0.01879469726172035 0.0 40-41 0.0 0.0 0.0 0.02509950016480276 0.0 42-43 0.0 0.0 0.0 0.033599012939211324 0.0 44-45 0.0 0.0 0.0 0.041140834133404844 0.0 46-47 0.0 0.0 0.0 0.05450861244057324 0.0 48-49 0.0 0.0 0.0 0.07110859983096743 0.0 50-51 0.0 0.0 0.0 0.07785234470831508 0.0 52-53 0.0 0.0 0.0 0.09189848788480248 0.0 54-55 0.0 0.0 0.0 0.12042971621204249 0.0 56-57 0.0 0.0 0.0 0.16376526021677348 0.0 58-59 0.0 0.0 0.0 0.20630272790465862 0.0 60-61 0.0 0.0 0.0 0.26192864718881603 0.0 62-63 0.0 0.0 0.0 0.31260649330852913 0.0 64-65 0.0 0.0 0.0 0.3626458783747655 0.0 66-67 0.0 0.0 0.0 0.4496761007268081 0.0 68-69 0.0 0.0 0.0 0.5608481316634384 0.0 70-71 0.0 0.0 0.0 0.6132418418643701 3.9903815842293734E-5 72-73 0.0 0.0 0.0 0.6835523653784916 7.980763168458747E-5 74-75 0.0 0.0 0.0 0.769704703782004 7.980763168458747E-5 76-77 0.0 0.0 0.0 0.8548195429736164 7.980763168458747E-5 78-79 0.0 0.0 0.0 0.9443238019078812 7.980763168458747E-5 80-81 0.0 0.0 0.0 1.1015448363265186 7.980763168458747E-5 82-83 0.0 0.0 0.0 1.2810321999851557 7.980763168458747E-5 84-85 0.0 0.0 0.0 1.5355786412431476 7.980763168458747E-5 86-87 0.0 0.0 0.0 1.8977855776436479 7.980763168458747E-5 88-89 0.0 0.0 0.0 2.2767521166979114 1.197114475268812E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3570 0.0 49.893955 1 GTATCAA 6335 0.0 41.763165 1 ATCAACG 8185 0.0 32.377876 3 TATCAAC 8235 0.0 32.18129 2 TCAACGC 8305 0.0 31.910046 4 CAACGCA 8435 0.0 31.425774 5 AACGCAG 8695 0.0 30.431435 6 ACGCAGA 9645 0.0 27.08927 7 CGCAGAG 9670 0.0 26.773607 8 GCAGAGT 10260 0.0 24.909885 9 TGGTATC 1900 0.0 24.496572 2 GTACATG 5465 0.0 24.336197 1 GTGGTAT 1935 0.0 24.301834 1 GAGTACT 6255 0.0 24.03237 12-13 TACATGG 5745 0.0 22.320637 2 GTACTTT 6720 0.0 22.2634 14-15 ACATGGG 5635 0.0 22.250658 3 AGTACTT 6590 0.0 21.441332 12-13 CAGAGTA 10130 0.0 20.704184 10-11 AGAGTAC 9460 0.0 20.689163 10-11 >>END_MODULE