FastQCFastQC Report
Thu 26 May 2016
SRR936812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292870
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53790.4160511110939228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38720.29948873436617757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.19398702112354685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.044.223521
GTATCAA53800.042.415121
ATCAACG66000.034.4373553
TCAACGC67000.033.9233634
TATCAAC67950.033.449092
CAACGCA68750.033.059865
AACGCAG71250.032.1580926
ACGCAGA82800.027.3799117
CGCAGAG82950.027.158518
GCAGAGT87750.024.9678429
TAAGCGG1000.001643763223.7747445
GTACATG58250.023.2600521
GAGTACT47100.022.30053912-13
TACATGG61550.022.0944822
ACATGGG60550.021.4384983
GTACTTT49400.021.16645614-15
AGAGTAC79450.020.39839710-11
AGTACTT48900.020.3619412-13
CATGGGG25900.020.1947614
CAGAGTA86250.019.75448210-11
Produced by FastQC (version 0.11.5)