##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936812_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1292870 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82014742394827 33.0 31.0 34.0 30.0 34.0 2 31.912558880629916 33.0 31.0 34.0 30.0 34.0 3 31.93600671374539 33.0 31.0 34.0 30.0 34.0 4 35.53241702568704 37.0 35.0 37.0 33.0 37.0 5 35.53114698306867 37.0 35.0 37.0 33.0 37.0 6 35.596525559414324 37.0 35.0 37.0 33.0 37.0 7 35.54341581133447 37.0 35.0 37.0 33.0 37.0 8 35.50490459210903 37.0 35.0 37.0 33.0 37.0 9 37.141968643405754 39.0 37.0 39.0 33.0 39.0 10-11 37.01844617014858 39.0 37.0 39.0 33.0 39.0 12-13 36.93884381260297 39.0 37.0 39.0 33.0 39.0 14-15 38.10935863621246 40.0 38.0 41.0 33.0 41.0 16-17 38.048268580754446 40.0 38.0 41.0 33.0 41.0 18-19 38.001505565138025 40.0 38.0 41.0 33.0 41.0 20-21 37.93641240031867 40.0 38.0 41.0 33.0 41.0 22-23 37.833965518574956 40.0 38.0 41.0 33.0 41.0 24-25 37.7922339446348 40.0 37.5 41.0 32.5 41.0 26-27 37.819354227416525 40.0 38.0 41.0 33.0 41.0 28-29 37.73922938887901 40.0 37.0 41.0 33.0 41.0 30-31 37.591744336244176 40.0 37.0 41.0 32.0 41.0 32-33 37.40984244355581 40.0 37.0 41.0 32.0 41.0 34-35 37.2484696837269 40.0 36.5 41.0 31.5 41.0 36-37 37.08305320720567 40.0 36.0 41.0 31.0 41.0 38-39 36.92781022067184 39.0 36.0 41.0 31.0 41.0 40-41 36.745958216990104 39.0 36.0 41.0 30.0 41.0 42-43 36.5185575502564 39.0 35.5 41.0 30.0 41.0 44-45 36.27212635454454 39.0 35.0 41.0 30.0 41.0 46-47 35.93923673687223 39.0 35.0 40.0 28.5 41.0 48-49 35.706412477665964 39.0 35.0 40.0 28.0 41.0 50-51 35.0194667677338 38.0 34.0 40.0 27.0 40.5 52-53 35.2059557418766 38.5 35.0 40.0 27.5 40.5 54-55 35.588481053779574 39.0 35.0 40.5 28.0 41.0 56-57 35.475972448892776 39.0 35.0 41.0 27.5 41.0 58-59 35.27272618283354 39.0 35.0 40.5 27.0 41.0 60-61 34.99459729129765 38.0 34.5 40.0 26.0 41.0 62-63 34.685813732239126 38.0 34.0 40.0 26.0 41.0 64-65 34.356748551671856 37.5 34.0 40.0 26.0 41.0 66-67 33.98742332949175 37.0 34.0 39.5 25.0 41.0 68-69 33.59432116144701 36.0 33.5 39.0 24.0 41.0 70-71 33.179448436424394 36.0 33.0 39.0 23.0 40.5 72-73 32.79743980446604 35.0 33.0 38.5 22.5 40.0 74-75 32.39846504288907 35.0 32.0 37.5 21.5 39.5 76-77 31.947777812154357 35.0 32.0 37.0 20.5 39.0 78-79 31.492181735209265 35.0 31.5 36.5 19.0 39.0 80-81 31.059010960111998 35.0 31.0 36.0 18.0 38.0 82-83 30.669418425673115 35.0 31.0 35.5 14.5 37.0 84-85 30.308497374059264 34.0 31.0 35.0 9.5 37.0 86-87 29.962691144507954 34.0 30.0 35.0 7.0 36.0 88-89 29.61170728688886 34.0 30.0 35.0 3.5 36.0 90-91 29.260267466953366 34.0 29.5 35.0 2.0 36.0 92-93 28.887541284119827 34.0 29.0 35.0 2.0 35.0 94-95 28.540277444754693 34.0 29.0 35.0 2.0 35.0 96-97 28.073635787047422 34.0 29.0 35.0 2.0 35.0 98-99 27.53289580545608 34.0 28.0 35.0 2.0 35.0 100-101 25.362850866676464 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28812039370230025 1101 2 0.26106239848835244 1101 3 0.3539537423975041 1101 4 0.46714613359813484 1101 5 0.33177497888055996 1101 6 0.2815300914032264 1101 7 0.15085555762502878 1101 8 0.1976913255983419 1101 9 0.22241198363376924 1101 10-11 0.3731901075846338 1101 12-13 0.5518721603163996 1101 14-15 0.6000716792607861 1101 16-17 0.5696290892907072 1101 18-19 0.577354087432397 1101 20-21 0.6017985706991524 1101 22-23 0.5641344093735938 1101 24-25 0.6553498823073838 1101 26-27 0.5889312836299752 1101 28-29 0.5021939379986833 1101 30-31 0.6133084034745053 1101 32-33 0.41296509766662837 1101 34-35 0.5600523662154373 1101 36-37 0.6167798321551459 1101 38-39 0.4921847008193794 1101 40-41 0.4713723168602115 1101 42-43 0.4036442026533891 1101 44-45 0.49119843949876696 1101 46-47 0.5674443556769759 1101 48-49 0.5191713409064604 1101 50-51 0.6478030032294058 1101 52-53 0.6918902191316718 1101 54-55 0.7562402470473373 1101 56-57 0.6673648438152924 1101 58-59 0.6988144109302041 1101 60-61 0.8469010428642534 1101 62-63 0.6448257287221679 1101 64-65 0.5068556227390459 1101 66-67 0.5512461919713232 1101 68-69 0.5372914531503241 1101 70-71 0.483465401570335 1101 72-73 0.3745343914037633 1101 74-75 0.40663177661004823 1101 76-77 0.533607961906668 1101 78-79 0.5919042349341517 1101 80-81 0.7769947041450145 1101 82-83 0.6882934146203823 1101 84-85 0.5475531648067964 1101 86-87 0.5736822406875675 1101 88-89 0.500457407319221 1101 90-91 0.46968762168124556 1101 92-93 0.4842554453738863 1101 94-95 0.4422235457864403 1101 96-97 0.5825984151560526 1101 98-99 0.7512083038078288 1101 100-101 1.1707144207786904 1102 1 0.0655146448112589 1102 2 0.028121238664084558 1102 3 0.08057473016537031 1102 4 0.06393953149977705 1102 5 0.11356331838066325 1102 6 -0.04151605678318049 1102 7 0.009901960500187101 1102 8 -0.03306092592094956 1102 9 0.08392073148483803 1102 10-11 -0.023747083339955566 1102 12-13 0.022581008628030474 1102 14-15 -0.16029892824261793 1102 16-17 -0.007617831451575796 1102 18-19 -0.07717459688797135 1102 20-21 -0.15074018090149366 1102 22-23 0.008174869511265115 1102 24-25 -0.12753874796236886 1102 26-27 -0.10883647560425658 1102 28-29 -0.34595815363267235 1102 30-31 -0.24043109945795038 1102 32-33 -0.2661946417018797 1102 34-35 -0.2366563123036869 1102 36-37 -0.18065718543959974 1102 38-39 0.02579388800852911 1102 40-41 -0.17705356517645043 1102 42-43 -0.031725520514086725 1102 44-45 0.027683310279599027 1102 46-47 -0.06397344706658004 1102 48-49 -0.22114783361900692 1102 50-51 -0.14000748711718103 1102 52-53 0.10404559341801445 1102 54-55 -0.1098307898896067 1102 56-57 0.06871717032626634 1102 58-59 -0.16259440696025962 1102 60-61 -0.06873490079293276 1102 62-63 -0.12984476770864717 1102 64-65 0.0026456530894520824 1102 66-67 -0.2068617536101769 1102 68-69 0.04154174851851877 1102 70-71 -0.15721564321950865 1102 72-73 -0.18014356882234495 1102 74-75 -0.03452433344379102 1102 76-77 -0.01046026637934716 1102 78-79 -0.04138181590455048 1102 80-81 -0.18947481456399728 1102 82-83 -0.06949139461067233 1102 84-85 -0.11837938512281099 1102 86-87 -0.07600859546974448 1102 88-89 -0.020906733919840548 1102 90-91 -0.252416178133398 1102 92-93 -0.1025805067729273 1102 94-95 -0.06488474645475151 1102 96-97 -0.19504398061611283 1102 98-99 -0.017555005447981387 1102 100-101 -0.4160672331417494 1103 1 0.12686245206857194 1103 2 0.07697428195096379 1103 3 0.115463726338632 1103 4 0.1463894253592457 1103 5 0.004050428873313194 1103 6 0.07931908105751262 1103 7 0.10593500250911347 1103 8 0.1027591960519203 1103 9 -0.08352287913916001 1103 10-11 0.22286334186114232 1103 12-13 0.025056851895030263 1103 14-15 0.2371769505228869 1103 16-17 0.2043417925482558 1103 18-19 0.23140698932724746 1103 20-21 0.2706973665777497 1103 22-23 0.2875812990005784 1103 24-25 0.14474881123138772 1103 26-27 0.26768357040180746 1103 28-29 0.16941660409003134 1103 30-31 0.11454525853737607 1103 32-33 0.19631148179031754 1103 34-35 0.33480235678634074 1103 36-37 0.13687037914993283 1103 38-39 0.27709579569822296 1103 40-41 0.20131542048925866 1103 42-43 0.2391914413818128 1103 44-45 0.13493210510532805 1103 46-47 0.2671617596412119 1103 48-49 0.21106155786561231 1103 50-51 0.12912993469736733 1103 52-53 0.19609144328773453 1103 54-55 0.399357820769886 1103 56-57 0.26112084513155764 1103 58-59 0.3403003186717868 1103 60-61 0.25634816570996577 1103 62-63 0.3517748161068681 1103 64-65 0.2666652553641953 1103 66-67 0.2118338893032501 1103 68-69 0.22236953816857152 1103 70-71 0.0714293433265567 1103 72-73 0.04796503530542395 1103 74-75 0.004240212068836513 1103 76-77 0.19128129030111296 1103 78-79 0.43147709173543447 1103 80-81 0.39114229145583934 1103 82-83 0.4041252286294892 1103 84-85 0.47375414605552635 1103 86-87 0.3481690816500489 1103 88-89 0.2472432969638625 1103 90-91 0.30172152766918714 1103 92-93 0.2573517975770585 1103 94-95 0.5619176901626659 1103 96-97 0.5540279109572523 1103 98-99 0.43697549131810476 1103 100-101 0.5810644876128102 1104 1 0.16145630619362805 1104 2 0.03065770030010384 1104 3 0.17318728565046726 1104 4 0.2104518967502571 1104 5 0.08629635579347195 1104 6 0.032199859513582396 1104 7 0.0818423536517372 1104 8 0.13041402652039835 1104 9 -0.01068928753862508 1104 10-11 0.18663655648249033 1104 12-13 0.013750951557646829 1104 14-15 -0.09966164225657081 1104 16-17 0.07835236512420352 1104 18-19 0.13758443925660657 1104 20-21 0.07456102391778074 1104 22-23 -0.17747826936000877 1104 24-25 -0.09911452475375171 1104 26-27 0.004083625854207185 1104 28-29 0.01000253121197403 1104 30-31 0.1523558377646168 1104 32-33 0.12134499051118297 1104 34-35 0.1134863636633412 1104 36-37 0.03876259896116352 1104 38-39 -0.04974511008911975 1104 40-41 -0.0486134890826051 1104 42-43 0.09349641987905954 1104 44-45 0.021786036975861123 1104 46-47 -0.06804763856943907 1104 48-49 0.06253954748435575 1104 50-51 -0.0322554262420951 1104 52-53 0.053791947254417494 1104 54-55 0.21710326210785524 1104 56-57 0.18428470361732963 1104 58-59 0.1427797211310562 1104 60-61 0.26761576502823914 1104 62-63 0.12392821897492468 1104 64-65 0.12596524725558567 1104 66-67 -0.08644323744024263 1104 68-69 0.11029571173982333 1104 70-71 0.07938866876526873 1104 72-73 -0.0233426810607682 1104 74-75 -0.002390535092494872 1104 76-77 0.04280542797702225 1104 78-79 0.027768469447384092 1104 80-81 0.02200267433898162 1104 82-83 0.22101272713948816 1104 84-85 0.2520708368175839 1104 86-87 0.45788487314154125 1104 88-89 0.4010336592317145 1104 90-91 0.217748691872945 1104 92-93 0.22701682994235384 1104 94-95 0.37756928017809344 1104 96-97 0.3383580485531965 1104 98-99 0.0753587548563921 1104 100-101 -0.0013557556528418502 1105 1 0.06291812009854425 1105 2 0.1910143954496455 1105 3 0.11579084213801494 1105 4 0.09080541085879901 1105 5 0.1865682092311971 1105 6 0.04384121388364548 1105 7 0.03146345399893136 1105 8 0.09617681713046977 1105 9 0.19488339401614496 1105 10-11 0.14836612195647803 1105 12-13 0.17078869084203774 1105 14-15 0.34358293584740096 1105 16-17 0.3467690228965168 1105 18-19 0.1494576747729326 1105 20-21 0.23785976343028636 1105 22-23 0.32646684931165737 1105 24-25 0.20583136615747577 1105 26-27 0.2920571981004585 1105 28-29 0.22354162767076247 1105 30-31 0.22800601533786136 1105 32-33 0.15451592333052133 1105 34-35 -0.05995445537100608 1105 36-37 -0.021767169932019215 1105 38-39 0.35058910849443947 1105 40-41 0.40526978764075494 1105 42-43 0.33946915919484866 1105 44-45 0.15568839994518413 1105 46-47 0.30760295756183353 1105 48-49 0.2399510172299344 1105 50-51 0.07009331211937564 1105 52-53 0.11470009260287384 1105 54-55 0.043331363675143564 1105 56-57 0.015719039187850115 1105 58-59 0.10069538587443816 1105 60-61 0.27345464948739107 1105 62-63 0.23681792265676904 1105 64-65 0.3466384559841984 1105 66-67 0.16832612662361868 1105 68-69 0.2200826447768165 1105 70-71 0.1007001784831445 1105 72-73 0.2502900468283755 1105 74-75 0.31736938002394055 1105 76-77 0.3137474555976638 1105 78-79 0.3370049986805199 1105 80-81 0.14984285214243087 1105 82-83 0.13693132597463986 1105 84-85 0.3469432917231856 1105 86-87 0.3483999297201237 1105 88-89 0.13517501883374905 1105 90-91 0.10860612891658405 1105 92-93 0.06132561275468973 1105 94-95 0.24450528893432733 1105 96-97 0.24629265304725223 1105 98-99 0.23299273004845844 1105 100-101 0.4776939502055022 1106 1 0.25982815752261246 1106 2 0.24841117438850446 1106 3 0.1479115137894773 1106 4 0.2863724502417213 1106 5 0.2599224268700482 1106 6 0.16792447671339517 1106 7 0.10530852903761456 1106 8 0.2913109263843481 1106 9 0.2556079422215447 1106 10-11 0.384451762853125 1106 12-13 0.38093892064561885 1106 14-15 0.1808682810100919 1106 16-17 0.13909442171814845 1106 18-19 0.195627233992127 1106 20-21 0.13105026030906686 1106 22-23 0.06009363000357837 1106 24-25 0.18755836102786105 1106 26-27 0.06358137130300179 1106 28-29 0.018982903900003123 1106 30-31 0.14435446311566835 1106 32-33 0.16057618293616116 1106 34-35 0.17607706925952016 1106 36-37 0.11021554335085426 1106 38-39 0.06745127970000908 1106 40-41 0.16963155869084545 1106 42-43 0.021447680372602917 1106 44-45 -0.17456030621872998 1106 46-47 -0.25743945891999687 1106 48-49 -0.3211378630206738 1106 50-51 -0.2602072237078872 1106 52-53 -0.1870796465719664 1106 54-55 -0.1919646370565644 1106 56-57 -0.21177431060902308 1106 58-59 -0.1449063336508587 1106 60-61 -0.12910177243655596 1106 62-63 0.016112734355033353 1106 64-65 -0.20518358534233272 1106 66-67 -0.27515896091515657 1106 68-69 -0.20772854280845365 1106 70-71 -0.1500959403897113 1106 72-73 -0.0711971596289942 1106 74-75 0.23947396923574615 1106 76-77 0.09133722381236353 1106 78-79 0.07420802614262101 1106 80-81 0.027856404074992014 1106 82-83 0.041475567927705015 1106 84-85 0.10994453411070637 1106 86-87 0.2192922383505227 1106 88-89 0.006031504020374001 1106 90-91 0.08868448317233302 1106 92-93 0.23272630940633832 1106 94-95 0.28387982685001134 1106 96-97 0.3066688754269933 1106 98-99 0.5124033962882919 1106 100-101 0.8513766764457955 1107 1 0.14322047645730862 1107 2 0.27213222437064033 1107 3 0.1967856637499601 1107 4 0.09354781488289632 1107 5 0.19541485096691957 1107 6 0.16322804441362138 1107 7 0.1290417476020025 1107 8 0.29142236497975915 1107 9 0.3402846229909571 1107 10-11 0.3131006403334382 1107 12-13 0.5005279043640556 1107 14-15 0.36358947061069813 1107 16-17 0.496395058711407 1107 18-19 0.6180938312338355 1107 20-21 0.6249890254413444 1107 22-23 0.6748353550474206 1107 24-25 0.5387388415972012 1107 26-27 0.5588644445488953 1107 28-29 0.5715518197049434 1107 30-31 0.6270452953057912 1107 32-33 0.6939891884606979 1107 34-35 0.6631607596446685 1107 36-37 0.6442926972859766 1107 38-39 0.5866187181109765 1107 40-41 0.6864911294088785 1107 42-43 0.7240484906720894 1107 44-45 0.661322024871744 1107 46-47 0.7788472533724402 1107 48-49 0.9033209232721262 1107 50-51 0.6573999462769606 1107 52-53 0.5946034989168894 1107 54-55 0.9554404447995637 1107 56-57 0.8488777460735193 1107 58-59 0.6235085740152186 1107 60-61 0.779681894508073 1107 62-63 0.8616162510753043 1107 64-65 0.8339313616474797 1107 66-67 0.8787806496133612 1107 68-69 0.8288655862989245 1107 70-71 0.8315906635383072 1107 72-73 0.9527669417724454 1107 74-75 0.9796904291005575 1107 76-77 0.9192001389691669 1107 78-79 0.9376230554945231 1107 80-81 0.9953025645030174 1107 82-83 0.9014881976351816 1107 84-85 0.9389557791616134 1107 86-87 0.9704360850827456 1107 88-89 0.9717029727938709 1107 90-91 0.9006680389345441 1107 92-93 1.0662991716746397 1107 94-95 1.0317413586311481 1107 96-97 0.9568278995835513 1107 98-99 1.16790346737843 1107 100-101 1.275455931062492 1108 1 0.01922313073403714 1108 2 -0.05443452584278674 1108 3 0.10212515891547014 1108 4 0.05440220369715831 1108 5 0.06390388902770638 1108 6 0.06129623795214911 1108 7 0.16332926193894792 1108 8 0.07118698431596471 1108 9 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56-57 -2.0547266427762523 2316 58-59 -2.021623939169338 2316 60-61 -2.210412208053768 2316 62-63 -2.3529570502985138 2316 64-65 -2.7607909092081897 2316 66-67 -2.539390049990253 2316 68-69 -2.3594270775807296 2316 70-71 -2.1346298420283674 2316 72-73 -2.4772056247675494 2316 74-75 -2.5061793866619695 2316 76-77 -2.7533759107889324 2316 78-79 -3.2279970124971307 2316 80-81 -3.165141295780309 2316 82-83 -3.7116767142389193 2316 84-85 -3.3541007718050437 2316 86-87 -3.853495074314722 2316 88-89 -3.5495122992546086 2316 90-91 -3.6621181198898256 2316 92-93 -3.8068368204454863 2316 94-95 -3.866607154634025 2316 96-97 -4.223223390154345 2316 98-99 -4.561014460223937 2316 100-101 -4.735786686276011 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2414.0 3 832.0 4 826.0 5 938.0 6 1027.0 7 1244.0 8 1455.0 9 1837.0 10 2328.0 11 3050.0 12 3986.0 13 4839.0 14 5608.0 15 7006.0 16 8690.0 17 11907.0 18 10552.0 19 6738.0 20 6425.0 21 6894.0 22 7543.0 23 8078.0 24 9234.0 25 10805.0 26 12537.0 27 15273.0 28 18257.0 29 22148.0 30 27192.0 31 34149.0 32 43843.0 33 58263.0 34 79964.0 35 115780.0 36 178042.0 37 270153.0 38 249603.0 39 43388.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00021666292665 19.048462853915023 14.42154867564786 22.529771807510464 2 15.238756220753174 20.50256567382208 40.69409368887568 23.56458441654906 3 19.124262094683985 25.31198984920813 29.816016897354757 25.747731158753123 4 12.619130720112553 17.04377045488491 37.72157443314479 32.61552439185775 5 13.815825132211632 37.38844280637094 34.06964049276417 14.72609156865326 6 31.049735310405186 36.45219128905963 18.04660240795889 14.451470992576299 7 28.107246681919364 33.20000123837859 20.911957474079188 17.78079460562286 8 26.06478527759367 34.78484399873638 20.149388940851455 19.000981782818492 9 26.558595382971806 17.065353708950717 20.642002260516822 35.734048647560655 10-11 24.485356419443953 27.125663879864693 27.49632570243241 20.892653998258943 12-13 25.689885142358605 24.57343511592207 27.98688314432954 21.74979659738979 14-15 22.764552368633815 25.698731591472836 26.552206855900383 24.984509183992966 16-17 21.895328471333784 29.246955058623392 26.821140797532006 22.036575672510818 18-19 21.774624884378856 27.538382348957185 29.556440532998952 21.130552233665007 20-21 22.61643312382911 26.684757222903542 29.506814337410802 21.191995315856545 22-23 22.342964143672912 26.68371531592875 29.417237964066295 21.556082576332038 24-25 22.149405255043746 26.925311296402445 29.45869197107846 21.466591477475344 26-27 21.98858237248569 27.089270748449813 29.24091457045054 21.681232308613964 28-29 21.658623825143714 27.084205978737813 29.573213063941655 21.683957132176822 30-31 21.859454684547334 26.92082181756863 29.51965241148428 21.700071086399756 32-33 21.69461738120479 27.105740606591294 29.3369761247357 21.86266588746822 34-35 22.206785909603198 27.08415360231194 29.12903098547534 21.58002950260952 36-37 22.122419995993127 26.89984134952687 29.077088968802865 21.900649685677134 38-39 21.847783333726532 27.174210256198037 29.236890073046396 21.741116337029034 40-41 22.109358834279305 26.90362692296536 29.29273551793156 21.69427872482378 42-43 21.925208972200597 27.03541224559885 29.00185680013892 22.03752198206163 44-45 22.073676898967694 27.298670981734702 28.728707629904537 21.898944489393067 46-47 22.194892167464136 27.37175887466179 28.29055148440872 22.142797473465354 48-49 22.261858675503913 27.594603134210434 28.068319769751053 22.075218420534604 50-51 22.165004871102393 27.688320608649946 27.947442371513315 22.199232148734342 52-53 22.463276530241266 27.677042319979577 27.829273575345965 22.030407574433195 54-55 22.13993908830494 27.661289511569276 27.895568237111572 22.30320316301421 56-57 22.180235638847996 28.129571197942976 27.658749896537664 22.03144326667136 58-59 22.351000549199792 27.680133664400252 27.753308735370787 22.21555705102917 60-61 22.38350934268956 27.579517476789274 28.102092388088444 21.934880792432722 62-63 22.159766014196336 27.662586406421152 28.23370422857709 21.943943350805426 64-65 22.13069168466982 27.674038871343388 28.026676381795273 22.16859306219152 66-67 22.005066648202167 28.064426659899787 27.66038680288619 22.27011988901186 68-69 21.986552687856676 27.87955071413454 27.977946624764893 22.155949973243896 70-71 22.01306222337973 27.843309044991805 27.91230810965395 22.23132062197452 72-73 22.046116420369504 27.789822983180112 27.79222085139792 22.371839745052462 74-75 22.007489929749998 28.02948782712357 27.632595815324727 22.330426427801704 76-77 22.171379454673602 27.96385740711943 27.633966114965258 22.230797023241706 78-79 22.061869947539712 28.02596360623179 27.51873015234412 22.393436293884378 80-81 21.940695550598747 28.264421162146125 27.51977724545614 22.27510604179899 82-83 22.119643809097894 28.09970652704586 27.549396380371277 22.23125328348497 84-85 22.078267175941594 27.938384180244647 27.457332108494896 22.52601653531886 86-87 22.24001838420908 27.805419859820407 27.65094143631276 22.303620319657757 88-89 22.199844140205713 27.79466129902251 27.535062649601027 22.470431911170753 90-91 22.000512283188908 27.89138203480121 27.827269252020116 22.28083642998977 92-93 22.035838155656705 27.780297062342235 27.796276892932813 22.387587889068246 94-95 21.903460802659907 27.74490456626056 27.754342345380945 22.597292285698593 96-97 21.965152238956076 27.855735143090886 27.603109183075492 22.576003434877546 98-99 21.828444036213078 27.87648026322153 27.44685716231311 22.84821853825228 100-101 21.768484713358045 27.91972074356902 27.59526861941789 22.716525923655045 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 305.0 1 215.0 2 155.0 3 197.5 4 196.0 5 205.0 6 355.5 7 692.5 8 1104.5 9 1346.0 10 1325.5 11 1305.0 12 1425.5 13 1542.5 14 1668.0 15 1791.0 16 1816.5 17 1896.0 18 2009.5 19 2142.0 20 2400.5 21 2769.0 22 3337.0 23 4022.0 24 4711.5 25 5806.5 26 7521.5 27 9505.5 28 11249.0 29 12929.5 30 15325.5 31 18089.0 32 20816.0 33 23839.5 34 27551.5 35 30464.5 36 33602.5 37 37204.5 38 38892.5 39 39373.0 40 39951.5 41 41553.0 42 43087.5 43 44466.0 44 46330.0 45 47886.0 46 47779.0 47 47794.0 48 50201.0 49 50346.0 50 48230.0 51 46586.5 52 44601.5 53 43466.0 54 41603.0 55 37830.5 56 33998.0 57 30426.5 58 27583.0 59 24561.0 60 20574.0 61 16237.5 62 12399.5 63 9427.5 64 7047.0 65 5284.0 66 3756.0 67 2790.5 68 1993.5 69 1297.5 70 891.5 71 592.5 72 410.0 73 283.0 74 204.5 75 160.5 76 104.5 77 54.5 78 27.5 79 15.5 80 14.0 81 12.0 82 6.0 83 7.0 84 7.5 85 4.5 86 3.5 87 5.5 88 4.5 89 3.0 90 3.0 91 2.0 92 2.0 93 2.0 94 3.5 95 3.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041767540433299555 2 0.03264055937565262 3 0.02784502695553304 4 0.053911066077795916 5 0.10673926999620999 6 0.1341975604662495 7 0.06636398091068707 8 0.10380007270645926 9 0.08740244572153426 10-11 0.08713173018168881 12-13 0.08484998491727706 14-15 0.07545228831978466 16-17 0.06257396335285063 18-19 0.0723197227872872 20-21 0.06586122347954551 22-23 0.03790017557836441 24-25 0.010132495919930078 26-27 0.0034419547208922783 28-29 0.008160139843913155 30-31 0.005955741876600123 32-33 0.006845235793235206 34-35 0.007966771601166397 36-37 0.007038604035981963 38-39 0.004756858771570227 40-41 0.00831483443811056 42-43 0.004215427691879307 44-45 0.002784502695553304 46-47 0.0024751135071584924 48-49 6.574520253389745E-4 50-51 0.0032099128295961696 52-53 0.0077347297098702885 54-55 0.013845166180667817 56-57 0.009977801325732671 58-59 0.006187783767896231 60-61 0.0031712391810468183 62-63 0.01098331618801581 64-65 0.0031712391810468183 66-67 0.023165515481061515 68-69 0.0496182910888179 70-71 0.06369549916078182 72-73 0.004254101340428659 74-75 0.01554680671683928 76-77 0.024983176962881035 78-79 0.028502478980872013 80-81 0.08078925181959518 82-83 0.0322538228901591 84-85 0.014115881720513278 86-87 0.03530904112555787 88-89 0.052828203918414064 90-91 0.04764593501280098 92-93 0.04764593501280098 94-95 0.014928028340049658 96-97 0.019143456031928966 98-99 0.04443602218320481 100-101 0.08925878085190313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1292870.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.373035755451326 #Duplication Level Percentage of deduplicated Percentage of total 1 82.64224175497226 51.54647499893537 2 10.4430653327459 13.027313747917486 3 2.8247875097058253 5.285717170333013 4 1.2394232338956006 3.0922635873562965 5 0.6639095766534221 2.070502788149522 6 0.4308743487359954 1.6124964695890225 7 0.2983039772647926 1.302428724795117 8 0.21118596413476193 1.0537847753621572 9 0.15672387021628723 0.8797809204659877 >10 0.9683046218821745 11.426020457967528 >50 0.08192338916621325 3.5275147569203678 >100 0.038262595263479915 4.005447442129618 >500 4.969126816814581E-4 0.17604564998333516 >1k 3.7268451126109354E-4 0.5774173343643546 >5k 1.2422817042036452E-4 0.4167911757307922 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5379 0.4160511110939228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3872 0.29948873436617757 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.19398702112354685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.734729709870289E-5 0.0 3 0.0 0.0 0.0 7.734729709870289E-5 0.0 4 0.0 0.0 0.0 7.734729709870289E-5 0.0 5 0.0 0.0 0.0 7.734729709870289E-5 0.0 6 0.0 0.0 0.0 7.734729709870289E-5 0.0 7 0.0 0.0 0.0 7.734729709870289E-5 0.0 8 0.0 0.0 0.0 7.734729709870289E-5 0.0 9 0.0 0.0 0.0 7.734729709870289E-5 0.0 10-11 0.0 0.0 0.0 7.734729709870289E-5 0.0 12-13 0.0 0.0 0.0 7.734729709870289E-5 0.0 14-15 0.0 0.0 0.0 7.734729709870289E-5 7.734729709870289E-5 16-17 0.0 0.0 0.0 7.734729709870289E-5 7.734729709870289E-5 18-19 0.0 0.0 0.0 7.734729709870289E-5 7.734729709870289E-5 20-21 0.0 0.0 0.0 7.734729709870289E-5 7.734729709870289E-5 22-23 0.0 0.0 0.0 3.0938918839481155E-4 7.734729709870289E-5 24-25 0.0 0.0 0.0 8.121466195363802E-4 7.734729709870289E-5 26-27 0.0 0.0 0.0 0.0012375567535792462 7.734729709870289E-5 28-29 0.0 0.0 0.0 0.002861849992652007 7.734729709870289E-5 30-31 0.0 0.0 0.0 0.00591706822805077 7.734729709870289E-5 32-33 0.0 0.0 0.0 0.00831483443811056 1.1602094564805433E-4 34-35 0.0 0.0 0.0 0.010441885108324889 2.3204189129610866E-4 36-37 0.0 0.0 0.0 0.01423190266616133 2.3204189129610866E-4 38-39 0.0 0.0 0.0 0.019259476977577017 2.3204189129610866E-4 40-41 0.0 0.0 0.0 0.024403072234640763 2.3204189129610866E-4 42-43 0.0 0.0 0.0 0.0321764755930604 2.3204189129610866E-4 44-45 0.0 0.0 0.0 0.03871232219790079 2.3204189129610866E-4 46-47 0.0 0.0 0.0 0.05414310796909202 2.3204189129610866E-4 48-49 0.0 0.0 0.0 0.07081145049386249 2.3204189129610866E-4 50-51 0.0 0.0 0.0 0.07831413831243667 2.3204189129610866E-4 52-53 0.0 0.0 0.0 0.09208195719600579 2.3204189129610866E-4 54-55 0.0 0.0 0.0 0.11741319699583097 2.3204189129610866E-4 56-57 0.0 0.0 0.0 0.1581752225668474 2.3204189129610866E-4 58-59 0.0 0.0 0.0 0.19723560760169237 2.3204189129610866E-4 60-61 0.0 0.0 0.0 0.25358311353809737 2.3204189129610866E-4 62-63 0.0 0.0 0.0 0.30586988637682055 2.3204189129610866E-4 64-65 0.0 0.0 0.0 0.3575378808387541 2.3204189129610866E-4 66-67 0.0 0.0 0.0 0.44072489886840904 2.3204189129610866E-4 68-69 0.0 0.0 0.0 0.5534972580383178 2.3204189129610866E-4 70-71 0.0 0.0 0.0 0.6061707673625345 2.3204189129610866E-4 72-73 0.0 0.0 0.0 0.6755899665086205 2.3204189129610866E-4 74-75 0.0 0.0 0.0 0.7552963561688337 2.3204189129610866E-4 76-77 0.0 0.0 0.0 0.8382513323071925 2.3204189129610866E-4 78-79 0.0 0.0 0.0 0.926891334782306 2.3204189129610866E-4 80-81 0.0 0.0 0.0 1.0752821242661676 2.3204189129610866E-4 82-83 0.0 0.0 0.0 1.2443246420753826 2.3204189129610866E-4 84-85 0.0 0.0 0.0 1.4845266732154045 2.3204189129610866E-4 86-87 0.0 0.0 0.0 1.8386612729818157 2.3204189129610866E-4 88-89 0.0 0.0 0.0 2.217895070656756 2.3204189129610866E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3010 0.0 44.22352 1 GTATCAA 5380 0.0 42.41512 1 ATCAACG 6600 0.0 34.437355 3 TCAACGC 6700 0.0 33.923363 4 TATCAAC 6795 0.0 33.44909 2 CAACGCA 6875 0.0 33.05986 5 AACGCAG 7125 0.0 32.158092 6 ACGCAGA 8280 0.0 27.379911 7 CGCAGAG 8295 0.0 27.15851 8 GCAGAGT 8775 0.0 24.967842 9 TAAGCGG 100 0.0016437632 23.774744 5 GTACATG 5825 0.0 23.260052 1 GAGTACT 4710 0.0 22.300539 12-13 TACATGG 6155 0.0 22.094482 2 ACATGGG 6055 0.0 21.438498 3 GTACTTT 4940 0.0 21.166456 14-15 AGAGTAC 7945 0.0 20.398397 10-11 AGTACTT 4890 0.0 20.36194 12-13 CATGGGG 2590 0.0 20.194761 4 CAGAGTA 8625 0.0 19.754482 10-11 >>END_MODULE