FastQCFastQC Report
Thu 26 May 2016
SRR936812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292870
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74250.574303680957869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58590.45317781370130017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.28881480736655657No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17170.13280530911847285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.045.392341
GTATCAA65500.040.2600251
ATCAACG82200.031.7805963
TCAACGC83250.031.3797574
TATCAAC83700.031.3269672
CAACGCA84550.030.8972785
AACGCAG87200.030.0695786
ACGCAGA98150.026.5697087
CGCAGAG98350.026.5650028
GCAGAGT104050.024.7445039
GAGTACT64500.022.83004412-13
GTACATG52850.021.9364911
GTACTTT67950.021.56520814-15
AGTACTT67100.021.41448412-13
AGAGTAC96450.021.0550110-11
GTCTACG1150.00371713620.6582971
TACATGG55450.020.559592
ACATGGG54100.020.5442243
CAGAGTA102900.020.1045510-11
GTATAGC3707.2759576E-1219.2624661
Produced by FastQC (version 0.11.5)