##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936812_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1292870 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.211304307470975 33.0 31.0 34.0 31.0 34.0 2 32.34071484371979 33.0 31.0 34.0 31.0 34.0 3 32.46151507885557 34.0 31.0 34.0 31.0 34.0 4 36.119056827059175 37.0 35.0 37.0 35.0 37.0 5 35.97086482012886 37.0 35.0 37.0 35.0 37.0 6 36.058960297632396 37.0 35.0 37.0 35.0 37.0 7 36.01884412199216 37.0 35.0 37.0 35.0 37.0 8 35.9694400829163 37.0 35.0 37.0 35.0 37.0 9 37.68307563792183 39.0 37.0 39.0 35.0 39.0 10-11 37.5974885332632 39.0 37.0 39.0 35.0 39.0 12-13 37.500230881681844 39.0 37.0 39.0 35.0 39.0 14-15 38.715682164486765 40.0 38.0 41.0 34.5 41.0 16-17 38.713113074013634 40.0 38.0 41.0 34.5 41.0 18-19 38.838017743469955 40.0 38.0 41.0 35.0 41.0 20-21 38.84264968635671 40.0 38.0 41.0 35.0 41.0 22-23 38.76010387742001 40.0 38.0 41.0 35.0 41.0 24-25 38.66612343081671 40.0 38.0 41.0 34.0 41.0 26-27 38.470007425340526 40.0 38.0 41.0 34.0 41.0 28-29 38.31830114396652 40.0 38.0 41.0 34.0 41.0 30-31 38.29022020775484 40.0 38.0 41.0 34.0 41.0 32-33 38.14984994624363 40.0 37.5 41.0 34.0 41.0 34-35 38.02095918383132 40.0 37.0 41.0 33.5 41.0 36-37 37.87031294716407 40.0 37.0 41.0 33.0 41.0 38-39 37.701715563049646 40.0 37.0 41.0 33.0 41.0 40-41 37.34558037544378 40.0 36.5 41.0 32.5 41.0 42-43 37.15711208396823 40.0 36.0 41.0 32.0 41.0 44-45 36.9166888395585 40.0 36.0 41.0 31.0 41.0 46-47 36.495223030931186 39.0 36.0 41.0 30.5 41.0 48-49 36.09333614361846 39.0 35.5 40.5 29.5 41.0 50-51 35.799834863520694 39.0 35.0 40.0 28.5 41.0 52-53 35.54040352084897 39.0 35.0 40.0 28.0 41.0 54-55 35.156293749564924 38.0 35.0 40.0 27.0 41.0 56-57 34.9641243899232 38.0 34.0 40.0 27.0 41.0 58-59 34.649397851292086 38.0 34.0 40.0 26.0 41.0 60-61 34.272113978977 37.5 34.0 40.0 26.0 41.0 62-63 33.8996987322778 37.0 33.0 39.5 25.0 41.0 64-65 33.585604121063994 36.5 33.0 39.0 24.5 40.0 66-67 33.25136595326676 36.0 33.0 39.0 23.0 40.0 68-69 32.81248694764361 35.5 32.0 39.0 22.0 40.0 70-71 32.37343197691956 35.0 32.0 38.0 21.5 40.0 72-73 31.948081400295468 35.0 31.0 37.5 20.0 39.5 74-75 31.460773318276395 35.0 31.0 37.0 19.0 39.0 76-77 30.039100218892848 33.5 29.5 35.5 18.0 37.5 78-79 30.716538399065644 34.0 30.5 36.0 18.0 38.0 80-81 30.58037080294229 34.0 31.0 35.5 17.5 37.0 82-83 30.23783675079474 34.0 30.0 35.0 13.0 37.0 84-85 29.80041535498542 34.0 30.0 35.0 7.5 36.0 86-87 29.42358357762188 34.0 29.0 35.0 6.0 36.0 88-89 29.04438651991306 34.0 29.0 35.0 2.0 35.5 90-91 28.59940519928531 34.0 29.0 35.0 2.0 35.0 92-93 28.13719244780991 33.0 29.0 35.0 2.0 35.0 94-95 27.60080093126146 33.0 27.0 34.5 2.0 35.0 96-97 26.840340482801828 33.0 25.5 34.0 2.0 35.0 98-99 25.988380502293346 32.0 24.5 34.0 2.0 35.0 100-101 22.821215203384718 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25170236236890275 1101 2 0.311844753209769 1101 3 0.31100541100681056 1101 4 0.1437552340949324 1101 5 0.07768185807908878 1101 6 0.19345848260489618 1101 7 0.19172761876463085 1101 8 0.3222168655874711 1101 9 0.2725583521387662 1101 10-11 0.24519601019502346 1101 12-13 0.3230171562224271 1101 14-15 0.5198966935627283 1101 16-17 0.4653872451816099 1101 18-19 0.35745852017880253 1101 20-21 0.1694215258048004 1101 22-23 0.20397421902443824 1101 24-25 0.1689282568936008 1101 26-27 0.2182398223269999 1101 28-29 0.37102059196027426 1101 30-31 0.3703030168400616 1101 32-33 0.47995625405914666 1101 34-35 0.2420440410172091 1101 36-37 0.2038701579192832 1101 38-39 0.3959114873082825 1101 40-41 0.5179778450186419 1101 42-43 0.35726096322219547 1101 44-45 0.19831120272140623 1101 46-47 0.1826850854482771 1101 48-49 0.3083203109227597 1101 50-51 0.39609399079038354 1101 52-53 0.45170324462107203 1101 54-55 0.29880341941552757 1101 56-57 0.4271834817246756 1101 58-59 0.5276193961252318 1101 60-61 0.4444055441683332 1101 62-63 0.5457469201222906 1101 64-65 0.4988187836845128 1101 66-67 0.5619637677943032 1101 68-69 0.3220066257044252 1101 70-71 0.16681932749148132 1101 72-73 0.3250678295917311 1101 74-75 0.4318797769069711 1101 76-77 0.36135294773628956 1101 78-79 0.48592513711506413 1101 80-81 0.34062639911560666 1101 82-83 0.6126674523424462 1101 84-85 0.43592376542397915 1101 86-87 0.14905244810816498 1101 88-89 0.30610285744656096 1101 90-91 0.4968778923806312 1101 92-93 0.4489495087995614 1101 94-95 0.406550534768364 1101 96-97 0.041300960794036 1101 98-99 0.2198201264856685 1101 100-101 0.40772102132899235 1102 1 -0.08765023298020225 1102 2 -0.02842118429244067 1102 3 0.06238143772083049 1102 4 0.03538163399871763 1102 5 -0.019573870649097103 1102 6 0.06989694343588582 1102 7 0.09207392471656561 1102 8 0.18836554721945475 1102 9 0.15286772822207695 1102 10-11 0.16290837462632624 1102 12-13 0.16996319050635833 1102 14-15 0.18198998746647277 1102 16-17 0.12138056667662767 1102 18-19 0.11974053137095808 1102 20-21 0.12529210147211955 1102 22-23 0.08515678840942797 1102 24-25 0.16598377196622494 1102 26-27 0.17561589148914436 1102 28-29 0.021327456529533606 1102 30-31 0.11789001780929453 1102 32-33 -0.03851628554141939 1102 34-35 0.06430366082350503 1102 36-37 0.1234818990706188 1102 38-39 0.2530095212526362 1102 40-41 0.3400880120520142 1102 42-43 0.2417566087520484 1102 44-45 0.1607106537256584 1102 46-47 0.16973752303184853 1102 48-49 0.22253831034263527 1102 50-51 0.15881569380915295 1102 52-53 0.09793844968088905 1102 54-55 0.25757072586976193 1102 56-57 0.09342024321573206 1102 58-59 0.0027743317809481027 1102 60-61 0.11769523847109298 1102 62-63 0.21280346102459902 1102 64-65 0.23889122848426325 1102 66-67 0.14984040646879038 1102 68-69 0.07479033835892324 1102 70-71 -0.057926514360957526 1102 72-73 -0.038184298120526705 1102 74-75 0.044317961825150576 1102 76-77 0.17786650637483703 1102 78-79 -0.1758344084070096 1102 80-81 0.171183919862127 1102 82-83 0.2931735047375845 1102 84-85 0.2565010245485553 1102 86-87 -0.06565175920847821 1102 88-89 0.01910560242530579 1102 90-91 0.10943263277497195 1102 92-93 0.0049477825758224014 1102 94-95 0.14952572383938545 1102 96-97 0.2397454138853199 1102 98-99 0.29874003394875714 1102 100-101 0.3098199042358374 1103 1 0.07873019740367937 1103 2 0.16549827669825135 1103 3 0.09849229245384805 1103 4 0.09980077195300652 1103 5 -0.0454309820647012 1103 6 0.059245261871680555 1103 7 0.07508469496313808 1103 8 0.10271652804316744 1103 9 0.1508645475559831 1103 10-11 0.09068826259304075 1103 12-13 0.15369759778781855 1103 14-15 0.15752601489971596 1103 16-17 0.1467016506589971 1103 18-19 0.1093096505110438 1103 20-21 0.15867028125075677 1103 22-23 0.19983666288671742 1103 24-25 0.19058991604755704 1103 26-27 0.1129372452601487 1103 28-29 -0.02565676793462046 1103 30-31 0.1770956677514306 1103 32-33 0.12127970454739767 1103 34-35 0.05244414260921815 1103 36-37 0.020945885836773925 1103 38-39 0.06450855214343676 1103 40-41 0.07424289913127069 1103 42-43 -0.14784960632629662 1103 44-45 0.07355614293056334 1103 46-47 0.043397454448736994 1103 48-49 0.033699040475895004 1103 50-51 -0.05217712839302635 1103 52-53 -0.04153375853914554 1103 54-55 0.06113122597270859 1103 56-57 -0.011645393520957725 1103 58-59 -0.13887766217010267 1103 60-61 -0.19062117390794953 1103 62-63 0.12508505646943036 1103 64-65 -0.013300804231157315 1103 66-67 -0.03512615393901086 1103 68-69 0.16337135659804858 1103 70-71 0.12691349387469586 1103 72-73 0.04649152952713109 1103 74-75 -0.22168281204226759 1103 76-77 -0.004326910782300075 1103 78-79 -0.11822068894460003 1103 80-81 -0.1251222831467267 1103 82-83 0.14691859706919885 1103 84-85 -0.052291422789409125 1103 86-87 -0.2081230586667715 1103 88-89 -0.047140111130154594 1103 90-91 -0.26738400324464706 1103 92-93 -0.2764928887719158 1103 94-95 0.03205974915692167 1103 96-97 0.43738991055201026 1103 98-99 0.20928912483203277 1103 100-101 0.7941883148257496 1104 1 -0.024270397850209235 1104 2 0.05353823232137955 1104 3 -0.041772018208149575 1104 4 -0.06575977373748998 1104 5 -0.1838097615812444 1104 6 -0.028454356500702715 1104 7 0.01620201602918314 1104 8 -0.02802633610330929 1104 9 0.11225263627534332 1104 10-11 0.0836915071564448 1104 12-13 0.07061315880059027 1104 14-15 0.13289360192430877 1104 16-17 0.0896747962264115 1104 18-19 0.20984833099048927 1104 20-21 0.0891158173884179 1104 22-23 0.02613649671634022 1104 24-25 -0.02894330476173934 1104 26-27 -6.364864436392281E-4 1104 28-29 0.08764007542617946 1104 30-31 0.08886338496211721 1104 32-33 0.07402334667164467 1104 34-35 0.0836600336833655 1104 36-37 -0.09598544398581765 1104 38-39 -0.02939377614749361 1104 40-41 0.2111406436309622 1104 42-43 0.22718780723017318 1104 44-45 0.196157705089 1104 46-47 -0.10285283841393067 1104 48-49 -0.09635832884569595 1104 50-51 0.12847327148829635 1104 52-53 0.05982811359972118 1104 54-55 0.14254219067635887 1104 56-57 0.05841707263868301 1104 58-59 -0.12612547798443074 1104 60-61 0.015777039358845002 1104 62-63 -0.007599202260117011 1104 64-65 -0.013169646594995754 1104 66-67 -0.2078482428653885 1104 68-69 -0.17563768954215675 1104 70-71 -0.17726703433559265 1104 72-73 -0.07450325817125503 1104 74-75 -0.17782851883432826 1104 76-77 0.12563124955809712 1104 78-79 0.2925549384541206 1104 80-81 0.143805987078359 1104 82-83 0.268411298015959 1104 84-85 0.17148200108628586 1104 86-87 -0.0305059126644025 1104 88-89 0.2334841693244769 1104 90-91 0.2625715040495429 1104 92-93 0.3737493044528044 1104 94-95 0.3984794334145292 1104 96-97 0.28570292815291864 1104 98-99 -0.027987930540778194 1104 100-101 0.4299372155865022 1105 1 -0.04118516478663281 1105 2 0.08106428232650842 1105 3 0.01646907540026632 1105 4 0.03890861320266481 1105 5 -0.05998899649902967 1105 6 0.09105484235831085 1105 7 0.0815745012802438 1105 8 0.09333791192580776 1105 9 0.10746433246081466 1105 10-11 0.0863914157015202 1105 12-13 0.07823886747164721 1105 14-15 0.13218582030276593 1105 16-17 0.005081707875085328 1105 18-19 -0.004964228261634673 1105 20-21 0.10759159648186056 1105 22-23 0.08427901990968678 1105 24-25 0.026117375688045286 1105 26-27 0.058579428806730505 1105 28-29 -0.0037162827841541457 1105 30-31 0.07865287120306874 1105 32-33 0.05402317924843203 1105 34-35 0.030251611040206683 1105 36-37 -0.07736505309447494 1105 38-39 0.04965551115282807 1105 40-41 0.26490609627007444 1105 42-43 0.23223833764783564 1105 44-45 0.04062635613927057 1105 46-47 -0.04490222407539335 1105 48-49 -0.12821122244196914 1105 50-51 -0.40881522590932207 1105 52-53 -0.2162132064485789 1105 54-55 -0.1335382645473473 1105 56-57 -0.10210244158286486 1105 58-59 -0.18377284703331753 1105 60-61 -0.31075221470796777 1105 62-63 -0.18695696682541296 1105 64-65 -0.38639407113003443 1105 66-67 -0.22364462275702124 1105 68-69 -0.24084944851133372 1105 70-71 -0.35093617677298283 1105 72-73 -0.35357556616299135 1105 74-75 -0.4152042855803124 1105 76-77 -0.3110386190774683 1105 78-79 -0.21575448475199366 1105 80-81 -0.40421371026232933 1105 82-83 -0.467272238631935 1105 84-85 -0.5196560791136164 1105 86-87 -0.3281582206980005 1105 88-89 -0.5117435367054739 1105 90-91 -0.3892671167180488 1105 92-93 -0.537105579386882 1105 94-95 -0.3574805189535688 1105 96-97 -0.050048414895147886 1105 98-99 -0.015357942113908507 1105 100-101 0.1269706855790318 1106 1 -0.07696592531072355 1106 2 -0.050150132417506654 1106 3 0.0846205094813186 1106 4 0.09363311567600618 1106 5 -0.10151827644780553 1106 6 0.11686449029770074 1106 7 0.06560639176721139 1106 8 0.17328211881763167 1106 9 0.1997058891799952 1106 10-11 0.07408263286018979 1106 12-13 0.16065412978310434 1106 14-15 0.1843836266884864 1106 16-17 0.0974093932995217 1106 18-19 0.12575628027882857 1106 20-21 0.06473532471149923 1106 22-23 0.12267336742758062 1106 24-25 0.16469084042481086 1106 26-27 0.1593248613176499 1106 28-29 0.15851894766729657 1106 30-31 0.10989243857781616 1106 32-33 0.2366488090169554 1106 34-35 0.06121068847220812 1106 36-37 0.12089789098283887 1106 38-39 0.23413633798840294 1106 40-41 0.15804019027994087 1106 42-43 0.1793362576014914 1106 44-45 0.03978259970819664 1106 46-47 0.20027190072739387 1106 48-49 0.15588926612882403 1106 50-51 0.4286446632666596 1106 52-53 0.3672317893212025 1106 54-55 0.4540249028548331 1106 56-57 0.32264012505255124 1106 58-59 0.2775058212492141 1106 60-61 0.050166694734947725 1106 62-63 0.12148135968622142 1106 64-65 0.021137998474152653 1106 66-67 0.23739547663398497 1106 68-69 0.14989514557855443 1106 70-71 0.2019796228901214 1106 72-73 0.18155122596194673 1106 74-75 0.047139177403398236 1106 76-77 0.33765230082283537 1106 78-79 0.3608124098127483 1106 80-81 0.2652212701872152 1106 82-83 0.40278469777764414 1106 84-85 0.28369455618085837 1106 86-87 0.16133865046717943 1106 88-89 0.398591907924434 1106 90-91 -0.012973852287675669 1106 92-93 0.2937528875221638 1106 94-95 0.31886914834572977 1106 96-97 0.3426649442005747 1106 98-99 0.37135613345069274 1106 100-101 1.0414570764814037 1107 1 0.20255985863417436 1107 2 0.2084982070833803 1107 3 0.15834098305691668 1107 4 0.06361770640815223 1107 5 0.043132863107899766 1107 6 0.13817872279960142 1107 7 0.09709868265012744 1107 8 0.1224763665366595 1107 9 0.09872202728187318 1107 10-11 0.24214228196951382 1107 12-13 0.09215461844774353 1107 14-15 0.09248905528517071 1107 16-17 0.17725130420243573 1107 18-19 0.2226242793412112 1107 20-21 0.19484084553322845 1107 22-23 0.11076320742994028 1107 24-25 0.1680516107088792 1107 26-27 0.38540078814732937 1107 28-29 0.26870481955901937 1107 30-31 0.16844381369165973 1107 32-33 0.006755149616850531 1107 34-35 0.17947265627285702 1107 36-37 0.19760735263195528 1107 38-39 0.24875656934516854 1107 40-41 0.3091425997873145 1107 42-43 0.1701435341575035 1107 44-45 0.07000039171254713 1107 46-47 0.04500815631373456 1107 48-49 0.09434634113468832 1107 50-51 -0.03447460549120507 1107 52-53 0.02559115701605208 1107 54-55 0.17044986305076293 1107 56-57 0.05939462326504952 1107 58-59 0.017178537622619672 1107 60-61 0.20711899004978562 1107 62-63 0.2636539511715412 1107 64-65 0.27786242107492853 1107 66-67 0.31313401350926284 1107 68-69 0.10213281976541566 1107 70-71 -0.004467420080345619 1107 72-73 0.19975798682624912 1107 74-75 0.195197519991261 1107 76-77 0.05735701416306327 1107 78-79 0.3614147546345059 1107 80-81 0.4158298914356102 1107 82-83 0.4422182694870962 1107 84-85 0.27985216622006703 1107 86-87 0.5757656575467323 1107 88-89 0.47386323528619556 1107 90-91 0.5030937999060612 1107 92-93 0.6616574832754587 1107 94-95 1.0965196897195462 1107 96-97 0.9338601423499249 1107 98-99 0.7730749777171759 1107 100-101 1.1416829637952084 1108 1 -0.027148779818787716 1108 2 0.13218464454944012 1108 3 0.06367488634730023 1108 4 0.03583602812488351 1108 5 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-1.5428963037611396 2316 90-91 -1.83126125557175 2316 92-93 -1.539939715942097 2316 94-95 -1.4102738073664334 2316 96-97 -0.9338130896215162 2316 98-99 -1.3074021730320773 2316 100-101 -2.464223354339719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 10.0 5 31.0 6 53.0 7 145.0 8 312.0 9 654.0 10 1123.0 11 1867.0 12 2919.0 13 4314.0 14 5564.0 15 7612.0 16 10457.0 17 15091.0 18 13503.0 19 8012.0 20 6558.0 21 6483.0 22 6744.0 23 7362.0 24 8433.0 25 10322.0 26 12596.0 27 15530.0 28 19369.0 29 24163.0 30 30307.0 31 38568.0 32 50006.0 33 65303.0 34 90091.0 35 130426.0 36 203012.0 37 297375.0 38 188674.0 39 9877.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09192228572136 19.41742915917842 15.164001327576376 22.326647227523846 2 15.565894710787271 20.800575486146563 40.194149224176606 23.43938057888956 3 19.27381604309179 25.154230749001442 30.42267269357738 25.14928051432938 4 12.97794828559716 17.44251162143139 37.38566135806384 32.193878734907614 5 13.594602287669666 37.463375318290524 34.02862216941874 14.913400224621068 6 31.248772111658557 36.618530865438906 17.58390248052782 14.548794542374715 7 27.94771005237967 33.37007335629005 20.830587500038675 17.85162909129161 8 26.423017335863108 34.48918645276513 19.95716503373505 19.130631177636715 9 26.685534265265854 16.67709566862536 20.587545992672112 36.049824073436675 10-11 24.520021347853998 27.17597283562926 27.335346941301136 20.968658875215606 12-13 25.8673656257699 24.45227511729572 28.108892506417376 21.571466750517008 14-15 22.830268072687556 25.4992377134963 26.941742128260604 24.72875208555554 16-17 21.71970113004401 28.865044436022185 27.4029484789654 22.012305954968404 18-19 21.47553543134424 26.830486994139736 30.663076691628135 21.03090088288789 20-21 22.26146783668702 25.991545842341175 30.867924090481917 20.879062230489883 22-23 21.934408477394904 25.944038364852844 30.82418687396063 21.297366283791625 24-25 21.89630447797213 26.094471026296695 30.869381264202982 21.13984323152819 26-27 21.787469244709627 26.300501134300745 30.629059292255118 21.28297032873451 28-29 21.378950279127597 26.37211469852866 30.881277470429104 21.36765755191464 30-31 21.636707480257105 26.184264465878236 30.83894745798108 21.340080595883578 32-33 21.587075561048938 26.253172693123854 30.684945916773437 21.474805829053768 34-35 21.897174503236986 26.262733298784873 30.578209719461352 21.26188247851679 36-37 21.802682532444905 26.103892537816776 30.42196457349312 21.6714603562452 38-39 21.622466262276834 26.302447650494553 30.728857042405238 21.346229044823374 40-41 21.838667048765124 26.12625260033855 30.484340731269928 21.550739619626402 42-43 21.708679140207447 26.286324224399976 30.279803847254556 21.725192788138017 44-45 21.876656940150017 26.452721974386193 29.97178730061124 21.698833784852546 46-47 22.0151841124882 26.74077600552881 29.402269147308168 21.841770734674824 48-49 22.237605758048865 27.009746157478503 28.935232005420502 21.817416079052123 50-51 22.129652870086076 27.057623712673873 28.836453262554002 21.97627015468604 52-53 22.4174044332514 27.1272506199405 28.631930919368287 21.82341402743981 54-55 22.079559429795726 27.091045503414886 28.682195425680852 22.147199641108543 56-57 22.197707340786803 27.554451719842476 28.417534538588896 21.83030640078183 58-59 22.339816214589828 27.08636197163435 28.59208588048341 21.981735933292416 60-61 22.292867507603393 26.922351033844766 29.03153822116203 21.753243237389817 62-63 22.140014904829915 26.988996182522673 29.1126057192934 21.758383193354007 64-65 22.067076375457848 26.973333348802797 28.89015480680765 22.069435468931704 66-67 22.005112656338223 27.365551060818177 28.60403598196261 22.025300300880986 68-69 22.009207436490037 27.086755956524566 28.890265672907056 22.013770934078345 70-71 22.0380095439605 27.16606096795514 28.76743373893296 22.028495749151396 72-73 22.13420055558843 27.07501603997638 28.545008811835125 22.24577459260007 74-75 22.089515064531337 27.283696937280205 28.30723344251314 22.31955455567532 76-77 22.15031066565909 27.28141830301446 28.42546305928907 22.142807972037385 78-79 22.12506497203109 27.23646880104945 28.33639114400277 22.302075082916687 80-81 21.997045253261245 27.51588563119037 28.201897380562873 22.285171734985514 82-83 22.13317822610025 27.37860525699046 28.20363083962876 22.28458567728053 84-85 21.981852926013605 27.235848921606205 28.216994482240644 22.56530367013955 86-87 22.043549067631655 27.10436948680197 28.316987833595736 22.535093611970638 88-89 22.05350972149868 27.09280141518938 28.192520210499367 22.66116865281257 90-91 21.908040020265386 27.094972715213345 28.497654397858984 22.499332866662282 92-93 21.797048427142713 26.915660507243572 28.530865438907238 22.756425626706474 94-95 21.673640814621734 27.02089150494636 28.450424249924588 22.855043430507322 96-97 21.920115402613575 26.91812370201065 28.324689739454623 22.83707115592115 98-99 21.741886380318938 26.853395232419363 28.24167715377648 23.163041233485217 100-101 21.34584898531096 27.034241428427226 28.311164344701506 23.30874524156031 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 428.0 1 313.5 2 225.5 3 247.5 4 220.0 5 233.0 6 413.5 7 873.5 8 1505.5 9 1823.5 10 1682.0 11 1562.5 12 1628.0 13 1776.0 14 2003.5 15 2168.5 16 2298.0 17 2438.0 18 2586.0 19 2767.0 20 3005.0 21 3304.0 22 3733.0 23 4474.5 24 5237.0 25 6347.5 26 7968.5 27 9788.0 28 11374.5 29 13228.0 30 15633.0 31 18119.0 32 20789.0 33 23291.5 34 26883.5 35 30031.5 36 33042.0 37 36406.5 38 38206.0 39 38935.5 40 39676.5 41 41487.0 42 42827.0 43 44071.5 44 45618.5 45 46930.5 46 47533.5 47 47742.0 48 49675.5 49 49803.5 50 48157.0 51 46591.0 52 44251.0 53 43132.5 54 41334.5 55 37531.0 56 33541.5 57 30075.5 58 27417.5 59 24682.0 60 20948.0 61 16379.5 62 12385.0 63 9452.5 64 6965.5 65 5205.5 66 3844.5 67 2898.0 68 2039.0 69 1258.5 70 809.5 71 534.5 72 409.0 73 290.5 74 208.0 75 160.0 76 100.0 77 54.0 78 28.5 79 14.5 80 8.0 81 5.5 82 5.0 83 3.5 84 2.0 85 0.5 86 1.0 87 0.5 88 0.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022353368861525134 2 0.0038673648549351443 3 1.5469459419740578E-4 4 0.0 5 0.002011029724566275 6 0.0 7 1.5469459419740578E-4 8 7.734729709870289E-5 9 7.734729709870289E-5 10-11 0.0 12-13 0.006999930387432611 14-15 0.0037900175578364417 16-17 0.0 18-19 8.121466195363802E-4 20-21 0.0011602094564805432 22-23 0.0015469459419740577 24-25 6.187783767896231E-4 26-27 2.3204189129610866E-4 28-29 1.933682427467572E-4 30-31 0.0 32-33 1.1602094564805433E-4 34-35 0.0 36-37 0.0018950087789182208 38-39 0.010867295242367755 40-41 7.347993224376774E-4 42-43 0.0 44-45 9.668412137337861E-4 46-47 4.2541013404286587E-4 48-49 1.1602094564805433E-4 50-51 0.001972356076016924 52-53 3.0938918839481155E-4 54-55 0.0 56-57 3.48062836944163E-4 58-59 2.707155398454601E-4 60-61 0.002011029724566275 62-63 3.8673648549351444E-5 64-65 3.8673648549351444E-5 66-67 0.0 68-69 1.5469459419740578E-4 70-71 8.121466195363802E-4 72-73 5.027574311415688E-4 74-75 0.00375134390928709 76-77 7.734729709870289E-5 78-79 4.6408378259221733E-4 80-81 0.002707155398454601 82-83 7.734729709870289E-5 84-85 0.0034419547208922783 86-87 5.027574311415688E-4 88-89 0.003055218235398764 90-91 0.0019336824274675721 92-93 0.0 94-95 0.0 96-97 3.867364854935144E-4 98-99 0.0010055148622831376 100-101 0.0027458290470039524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1292870.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.19276185617309 #Duplication Level Percentage of deduplicated Percentage of total 1 81.70061203638126 49.177854838094866 2 10.873625312799316 13.090270779331698 3 3.024811181961837 5.46215217407155 4 1.3162189672585776 3.1690741938709466 5 0.7472686713865795 2.2490082589675624 6 0.46068164602175493 1.6637820366298395 7 0.30526785648179644 1.2862440771287242 8 0.229075542583662 1.1030951665449606 9 0.17370949700924299 0.9410448947069678 >10 1.0366766959515903 11.788352667057387 >50 0.08541821078830177 3.5624515093168205 >100 0.04495503450039624 4.605424974339153 >500 0.0011626247600677651 0.4444608875520167 >1k 2.583610577928367E-4 0.4238551955993776 >5k 2.583610577928367E-4 1.0329283467881367 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7425 0.574303680957869 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5859 0.45317781370130017 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.28881480736655657 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1717 0.13280530911847285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.734729709870289E-5 0.0 4 0.0 0.0 0.0 1.5469459419740578E-4 0.0 5 0.0 0.0 0.0 1.5469459419740578E-4 0.0 6 0.0 0.0 0.0 1.5469459419740578E-4 0.0 7 0.0 0.0 0.0 1.5469459419740578E-4 0.0 8 0.0 0.0 0.0 1.5469459419740578E-4 0.0 9 0.0 0.0 0.0 1.5469459419740578E-4 0.0 10-11 0.0 0.0 0.0 1.5469459419740578E-4 0.0 12-13 0.0 0.0 0.0 1.5469459419740578E-4 7.734729709870289E-5 14-15 0.0 0.0 0.0 1.5469459419740578E-4 7.734729709870289E-5 16-17 0.0 0.0 0.0 1.9336824274675723E-4 7.734729709870289E-5 18-19 0.0 0.0 0.0 2.3204189129610866E-4 7.734729709870289E-5 20-21 0.0 0.0 0.0 2.3204189129610866E-4 1.1602094564805433E-4 22-23 0.0 0.0 0.0 3.4806283694416296E-4 1.5469459419740578E-4 24-25 0.0 0.0 0.0 8.121466195363802E-4 1.5469459419740578E-4 26-27 0.0 0.0 0.0 0.0012762304021285975 1.5469459419740578E-4 28-29 0.0 0.0 0.0 0.0027845026955533037 1.5469459419740578E-4 30-31 0.0 0.0 0.0 0.005839720930952068 1.5469459419740578E-4 32-33 0.0 0.0 0.0 0.00831483443811056 1.5469459419740578E-4 34-35 0.0 0.0 0.0 0.010519232405423594 1.5469459419740578E-4 36-37 0.0 0.0 0.0 0.01423190266616133 1.5469459419740578E-4 38-39 0.0 0.0 0.0 0.019220803329027668 1.5469459419740578E-4 40-41 0.0 0.0 0.0 0.02436439858609141 1.5469459419740578E-4 42-43 0.0 0.0 0.0 0.03198310735031364 1.5469459419740578E-4 44-45 0.0 0.0 0.0 0.038480280306604685 1.5469459419740578E-4 46-47 0.0 0.0 0.0 0.05329228770100629 1.5469459419740578E-4 48-49 0.0 0.0 0.0 0.06972858833448065 1.5469459419740578E-4 50-51 0.0 0.0 0.0 0.07730862345015353 1.5469459419740578E-4 52-53 0.0 0.0 0.0 0.09072837949677848 1.5469459419740578E-4 54-55 0.0 0.0 0.0 0.11547951456836342 1.5469459419740578E-4 56-57 0.0 0.0 0.0 0.15612551919373177 3.0938918839481155E-4 58-59 0.0 0.0 0.0 0.19468314679743517 3.0938918839481155E-4 60-61 0.0 0.0 0.0 0.250566568951248 3.4806283694416296E-4 62-63 0.0 0.0 0.0 0.3034334465182114 3.867364854935144E-4 64-65 0.0 0.0 0.0 0.35463735719755274 3.867364854935144E-4 66-67 0.0 0.0 0.0 0.43786304887575705 3.867364854935144E-4 68-69 0.0 0.0 0.0 0.5504807134514684 3.867364854935144E-4 70-71 0.0 0.0 0.0 0.6031542227756851 3.867364854935144E-4 72-73 0.0 0.0 0.0 0.6726507692188697 4.2541013404286587E-4 74-75 0.0 0.0 0.0 0.7527438953645764 4.6408378259221733E-4 76-77 0.0 0.0 0.0 0.8356988715029353 4.6408378259221733E-4 78-79 0.0 0.0 0.0 0.9240681584382033 4.6408378259221733E-4 80-81 0.0 0.0 0.0 1.0721108850851206 4.6408378259221733E-4 82-83 0.0 0.0 0.0 1.241733507622576 4.6408378259221733E-4 84-85 0.0 0.0 0.0 1.481278086737259 4.6408378259221733E-4 86-87 0.0 0.0 0.0 1.8336723723189494 5.027574311415688E-4 88-89 0.0 0.0 0.0 2.2112432031062674 5.414310796909202E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3695 0.0 45.39234 1 GTATCAA 6550 0.0 40.260025 1 ATCAACG 8220 0.0 31.780596 3 TCAACGC 8325 0.0 31.379757 4 TATCAAC 8370 0.0 31.326967 2 CAACGCA 8455 0.0 30.897278 5 AACGCAG 8720 0.0 30.069578 6 ACGCAGA 9815 0.0 26.569708 7 CGCAGAG 9835 0.0 26.565002 8 GCAGAGT 10405 0.0 24.744503 9 GAGTACT 6450 0.0 22.830044 12-13 GTACATG 5285 0.0 21.936491 1 GTACTTT 6795 0.0 21.565208 14-15 AGTACTT 6710 0.0 21.414484 12-13 AGAGTAC 9645 0.0 21.05501 10-11 GTCTACG 115 0.003717136 20.658297 1 TACATGG 5545 0.0 20.55959 2 ACATGGG 5410 0.0 20.544224 3 CAGAGTA 10290 0.0 20.10455 10-11 GTATAGC 370 7.2759576E-12 19.262466 1 >>END_MODULE