FastQCFastQC Report
Thu 26 May 2016
SRR936811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564447
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85660.33402912986698496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63180.24636890526495578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40370.15742185352241633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.047.0368771
GTATCAA84550.041.1513021
ATCAACG104000.032.94993
TCAACGC105400.032.512874
TATCAAC106200.032.4041182
CAACGCA108250.031.6562565
AACGCAG111450.030.91616
ACGCAGA127450.026.8111727
CGCAGAG128400.026.5033138
GCAGAGT139700.023.3057639
GTACATG86900.023.191681
TAGTGCG1954.199901E-721.936999
TACATGG90000.021.7062822
GAGTACT76850.020.98774312-13
ACATGGG90400.020.767343
GTGGTAT26300.020.0610371
AGAGTAC127950.019.88701410-11
ATTCGCG3150.019.60049480-81
CAGAGTA135450.019.5050110-11
GTACTTT83500.019.31739614-15
Produced by FastQC (version 0.11.5)