##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936811_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2564447 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.578557092425775 33.0 31.0 34.0 30.0 34.0 2 31.679664270698517 33.0 31.0 34.0 30.0 34.0 3 31.68993471107026 33.0 31.0 34.0 30.0 34.0 4 35.26722603352692 37.0 35.0 37.0 33.0 37.0 5 35.28208303778553 37.0 35.0 37.0 33.0 37.0 6 35.33100196650584 37.0 35.0 37.0 33.0 37.0 7 35.25806733381505 37.0 35.0 37.0 32.0 37.0 8 35.2265092630107 37.0 35.0 37.0 32.0 37.0 9 36.823907454511634 39.0 37.0 39.0 33.0 39.0 10-11 36.68041998918285 39.0 37.0 39.0 32.0 39.0 12-13 36.5582398466414 39.0 37.0 39.0 32.0 39.0 14-15 37.686506876531276 40.0 37.0 41.0 32.0 41.0 16-17 37.60365197643001 40.0 37.0 41.0 32.0 41.0 18-19 37.54193730656161 40.0 37.0 41.0 32.0 41.0 20-21 37.46986367821211 40.0 37.0 41.0 32.0 41.0 22-23 37.32311215634404 40.0 37.0 41.0 31.5 41.0 24-25 37.13815142211946 39.5 36.5 41.0 31.0 41.0 26-27 37.09759414017915 39.0 36.5 41.0 31.0 41.0 28-29 37.18171208061621 40.0 37.0 41.0 31.0 41.0 30-31 37.056997668503186 39.5 36.5 41.0 31.0 41.0 32-33 36.885339997278166 39.0 36.0 41.0 30.0 41.0 34-35 36.69227420180647 39.0 36.0 41.0 30.0 41.0 36-37 36.544361220957185 39.0 35.5 41.0 30.0 41.0 38-39 36.381635494903975 39.0 35.0 41.0 30.0 41.0 40-41 36.176907730984496 39.0 35.0 41.0 29.5 41.0 42-43 35.977999545321076 39.0 35.0 40.0 29.0 41.0 44-45 35.75158620942449 39.0 35.0 40.0 27.5 41.0 46-47 35.43938244775579 38.0 35.0 40.0 26.5 41.0 48-49 35.12680628611159 38.0 34.0 40.0 26.0 41.0 50-51 34.484043538431486 37.5 33.5 39.5 24.5 40.5 52-53 34.79851504047461 38.0 34.0 40.0 26.0 40.5 54-55 35.09818276610903 39.0 35.0 40.0 26.0 41.0 56-57 34.97384270370961 38.0 34.0 40.0 26.0 41.0 58-59 34.71892341701739 38.0 34.0 40.0 25.5 41.0 60-61 34.4299383063873 38.0 34.0 40.0 24.5 41.0 62-63 34.10275763156735 37.5 34.0 40.0 23.5 41.0 64-65 33.78416867262221 37.0 33.0 40.0 23.0 41.0 66-67 33.40822095367929 36.5 33.0 39.0 22.0 41.0 68-69 32.964962816544855 36.0 32.0 39.0 21.5 41.0 70-71 32.60534064459121 35.0 32.0 39.0 20.5 40.0 72-73 32.214599872799084 35.0 32.0 38.0 20.0 40.0 74-75 31.774286815052136 35.0 31.0 37.0 18.5 39.5 76-77 31.328754698381367 35.0 31.0 37.0 17.5 39.0 78-79 30.90167295327219 35.0 31.0 36.0 13.5 39.0 80-81 30.4761285766483 34.5 30.0 36.0 9.5 37.5 82-83 30.056829991027307 34.0 30.0 35.0 7.0 37.0 84-85 29.711086639731683 34.0 30.0 35.0 4.5 36.5 86-87 29.365643548102184 34.0 29.0 35.0 2.0 36.0 88-89 28.994161899232076 34.0 29.0 35.0 2.0 36.0 90-91 28.647100524986477 34.0 29.0 35.0 2.0 35.5 92-93 28.273302782237263 34.0 29.0 35.0 2.0 35.0 94-95 27.859322302235142 34.0 27.5 35.0 2.0 35.0 96-97 27.386622340021063 34.0 27.0 35.0 2.0 35.0 98-99 26.746493298555205 33.5 25.5 35.0 2.0 35.0 100-101 24.508728002567416 31.0 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41216500647397325 1101 2 0.32079412210454805 1101 3 0.43072200372310476 1101 4 0.34112882487476526 1101 5 0.30199708900220656 1101 6 0.29114909659934085 1101 7 0.3237114894912594 1101 8 0.27647321663579305 1101 9 0.40967289503550575 1101 10-11 0.4260593840436897 1101 12-13 0.5282319320095823 1101 14-15 0.7873566578280276 1101 16-17 0.7620899869510822 1101 18-19 0.7520188900418532 1101 20-21 0.7286545416882717 1101 22-23 0.7538302472770084 1101 24-25 0.6815265276786491 1101 26-27 0.819425810593529 1101 28-29 0.7987591984579367 1101 30-31 0.71463752482628 1101 32-33 0.7061305055164979 1101 34-35 0.7767165232483109 1101 36-37 0.7229748854458435 1101 38-39 0.6913023171881107 1101 40-41 0.7600366062418047 1101 42-43 0.9494251273058012 1101 44-45 0.8741148908266538 1101 46-47 0.8200639457220902 1101 48-49 0.803785048142565 1101 50-51 0.8321529109483521 1101 52-53 0.9941717082058901 1101 54-55 1.0123569020254024 1101 56-57 0.9016938316885046 1101 58-59 0.8901448014998934 1101 60-61 0.9019818764228447 1101 62-63 1.0071404232816477 1101 64-65 0.8749680820489445 1101 66-67 0.8618153921153109 1101 68-69 0.8990972170578004 1101 70-71 0.8434810516302917 1101 72-73 0.8815553268440297 1101 74-75 0.8702680620947767 1101 76-77 0.883224377717454 1101 78-79 0.7737866623085878 1101 80-81 0.8680330242756966 1101 82-83 0.8068477217036296 1101 84-85 0.9614664038605802 1101 86-87 1.129749635256644 1101 88-89 1.1485882906676572 1101 90-91 1.1004080757152614 1101 92-93 0.9921094921451079 1101 94-95 1.1294550066900584 1101 96-97 1.0679983151130799 1101 98-99 1.0668002149071505 1101 100-101 1.3363205488790655 1102 1 0.3032312210174055 1102 2 0.4323839208587721 1102 3 0.28924243611679046 1102 4 0.29852391454819127 1102 5 0.32246850937567473 1102 6 0.14158537096903245 1102 7 0.22899619740675092 1102 8 0.25450462817054387 1102 9 0.2906039892997896 1102 10-11 0.3286096817458457 1102 12-13 0.2985426421413493 1102 14-15 0.5892485451145646 1102 16-17 0.4084895278653349 1102 18-19 0.4166297390206708 1102 20-21 0.3567784629577062 1102 22-23 0.4135369947160896 1102 24-25 0.5064884766603299 1102 26-27 0.4249016805588113 1102 28-29 0.5913199289275539 1102 30-31 0.4236220587157291 1102 32-33 0.5073361964867118 1102 34-35 0.42480843439059157 1102 36-37 0.3952878706684686 1102 38-39 0.36972833798328253 1102 40-41 0.4771284530725026 1102 42-43 0.36498422854872103 1102 44-45 0.5083317159453244 1102 46-47 0.46030427864719314 1102 48-49 0.47327498860212813 1102 50-51 0.19699552896834405 1102 52-53 0.4690082221224756 1102 54-55 0.6535524955278404 1102 56-57 0.7680327245838896 1102 58-59 0.44550181871780836 1102 60-61 0.49040143582805484 1102 62-63 0.4763611483204997 1102 64-65 0.5509002426761853 1102 66-67 0.3964664165932916 1102 68-69 0.4911578013147633 1102 70-71 0.5055512496440002 1102 72-73 0.4767390078629816 1102 74-75 0.5552577117780579 1102 76-77 0.622684355576677 1102 78-79 0.5960122772262366 1102 80-81 0.5802707896846435 1102 82-83 0.6277747374044509 1102 84-85 0.6806764103027341 1102 86-87 0.6415632465552505 1102 88-89 0.6156069624631293 1102 90-91 0.5488388456732913 1102 92-93 0.6582978299820823 1102 94-95 0.6223720068241789 1102 96-97 0.4969489974754353 1102 98-99 0.5689318414734394 1102 100-101 0.9602863417144256 1103 1 0.14551821055908576 1103 2 0.3134823189582896 1103 3 0.2231012447389027 1103 4 0.22421305107583578 1103 5 0.17505644508813134 1103 6 0.10626718748675046 1103 7 0.10133890566805093 1103 8 0.16630323738899477 1103 9 0.11603248140622213 1103 10-11 0.2096794781116671 1103 12-13 -0.010619090620323846 1103 14-15 0.1057035674264597 1103 16-17 0.22572216140110868 1103 18-19 0.23752008938047453 1103 20-21 0.22001819277429036 1103 22-23 0.17506578153478358 1103 24-25 0.4367003735578123 1103 26-27 0.3613657893237061 1103 28-29 0.42117558805387745 1103 30-31 0.45719884153169943 1103 32-33 0.48570375190035264 1103 34-35 0.3626098331043508 1103 36-37 0.37587784434978744 1103 38-39 0.43791309145414203 1103 40-41 0.48390210632491915 1103 42-43 0.6573317012232565 1103 44-45 0.6537084808629743 1103 46-47 0.6118382976763783 1103 48-49 0.6843587070207278 1103 50-51 0.5786351989882377 1103 52-53 0.5591996579752632 1103 54-55 0.6230504626437323 1103 56-57 0.5871837081784363 1103 58-59 0.6309852058139285 1103 60-61 0.5803742273165895 1103 62-63 0.6172919326334281 1103 64-65 0.6734654088662424 1103 66-67 0.750277235319821 1103 68-69 0.788054602191778 1103 70-71 0.6655082885687875 1103 72-73 0.6117110976556503 1103 74-75 0.5782440988782405 1103 76-77 0.4965002438016093 1103 78-79 0.6131389953641602 1103 80-81 0.5792493899946081 1103 82-83 0.5379126664050311 1103 84-85 0.7197509548105891 1103 86-87 0.6283336099726959 1103 88-89 0.6137681887834354 1103 90-91 0.4784732417877251 1103 92-93 0.5241354870500956 1103 94-95 0.5030784976126412 1103 96-97 0.45703546877912515 1103 98-99 0.5954386570759027 1103 100-101 0.9464751365466917 1104 1 0.28431340069247213 1104 2 0.3204776276817576 1104 3 0.2798104542566371 1104 4 0.24432333525555094 1104 5 0.26333841278496806 1104 6 0.18132263460275055 1104 7 0.2665802451590835 1104 8 0.2492813237494218 1104 9 0.319441907913685 1104 10-11 0.31949527039984105 1104 12-13 0.3731464226391239 1104 14-15 0.34014838283714965 1104 16-17 0.40768652720070975 1104 18-19 0.4907252887616451 1104 20-21 0.4111457225449726 1104 22-23 0.5349948418985662 1104 24-25 0.3140089683776779 1104 26-27 0.3902736527955355 1104 28-29 0.34444000845187617 1104 30-31 0.3574579095504973 1104 32-33 0.40801786909725735 1104 34-35 0.4074616937765825 1104 36-37 0.40519040986978183 1104 38-39 0.38589803005454115 1104 40-41 0.39120829922345024 1104 42-43 0.41541425584654945 1104 44-45 0.3828684312113566 1104 46-47 0.3062357332105705 1104 48-49 0.2826967101894269 1104 50-51 0.31899790442462006 1104 52-53 0.47872763576425825 1104 54-55 0.3268021820850251 1104 56-57 0.38341814131607777 1104 58-59 0.4080096285806718 1104 60-61 0.5081560976347888 1104 62-63 0.5767455350942683 1104 64-65 0.4606256971682754 1104 66-67 0.3838482465542299 1104 68-69 0.5228800621495111 1104 70-71 0.4849472982727079 1104 72-73 0.6038748612136757 1104 74-75 0.5338179347312995 1104 76-77 0.4165616982734619 1104 78-79 0.36490808317263657 1104 80-81 0.44985793884358927 1104 82-83 0.4416797264916106 1104 84-85 0.5751364657370353 1104 86-87 0.49804201263885517 1104 88-89 0.5091579419981436 1104 90-91 0.5899345799157096 1104 92-93 0.4997465411974673 1104 94-95 0.5075480877825811 1104 96-97 0.4502951568050264 1104 98-99 0.47828711370743804 1104 100-101 0.6203838129961454 1105 1 0.34911868414520697 1105 2 0.4243363809197582 1105 3 0.40733899088270675 1105 4 0.31963954640924186 1105 5 0.3783641597026204 1105 6 0.37968467286265906 1105 7 0.4763132777139063 1105 8 0.3783937211524133 1105 9 0.4603936850242434 1105 10-11 0.5055544395542881 1105 12-13 0.41361728869925685 1105 14-15 0.6118076381614586 1105 16-17 0.7008131752706817 1105 18-19 0.5921052305235435 1105 20-21 0.6140796185936068 1105 22-23 0.5883601788484185 1105 24-25 0.6577218530157367 1105 26-27 0.5452133440936464 1105 28-29 0.6122340641187449 1105 30-31 0.4269158350355937 1105 32-33 0.5226962029671185 1105 34-35 0.4903801918587618 1105 36-37 0.5145887998153356 1105 38-39 0.578119241793722 1105 40-41 0.7197037579657533 1105 42-43 0.7498938686370593 1105 44-45 0.6149844112731486 1105 46-47 0.6464526593048205 1105 48-49 0.7533528536883622 1105 50-51 0.6250698195375151 1105 52-53 0.6211518893158043 1105 54-55 0.7242000726370605 1105 56-57 0.8194482403828474 1105 58-59 0.8004303121214562 1105 60-61 0.7987802701572875 1105 62-63 0.8224679481930579 1105 64-65 0.8393387633668894 1105 66-67 0.9639880522558855 1105 68-69 1.087939530368864 1105 70-71 0.9230849276957969 1105 72-73 0.9051525578672113 1105 74-75 0.9257947129332571 1105 76-77 0.7602649948826041 1105 78-79 0.8649190992058635 1105 80-81 0.9547510976013562 1105 82-83 1.0152390843466321 1105 84-85 1.0464658112663408 1105 86-87 1.1207024825828285 1105 88-89 1.0970305950220656 1105 90-91 1.0460742436329369 1105 92-93 0.9219847706679687 1105 94-95 0.9759008603345407 1105 96-97 1.1196238275002983 1105 98-99 1.1459792914670466 1105 100-101 1.3102969617742026 1106 1 0.6053174113186337 1106 2 0.5543951011983417 1106 3 0.5698185690285413 1106 4 0.4390320753422259 1106 5 0.5546824287820087 1106 6 0.5859456516548107 1106 7 0.48404882598971 1106 8 0.5194209114644579 1106 9 0.6398055691922551 1106 10-11 0.6632282732973209 1106 12-13 0.6741233612951447 1106 14-15 0.8320163195516628 1106 16-17 0.7824332044143105 1106 18-19 0.7798300303667176 1106 20-21 0.8417237514834426 1106 22-23 0.8449628114423575 1106 24-25 0.8773623142517266 1106 26-27 0.9677641230528948 1106 28-29 0.9175183353216383 1106 30-31 0.8619155950253088 1106 32-33 0.9505601047153007 1106 34-35 0.8761582061781539 1106 36-37 0.896117792889946 1106 38-39 0.9015153940428178 1106 40-41 0.9168445976794999 1106 42-43 0.9135112298062182 1106 44-45 1.0205879465071632 1106 46-47 0.9783287273925438 1106 48-49 0.9703620133536077 1106 50-51 0.8523643430398948 1106 52-53 1.0175876190930992 1106 54-55 1.0968776374490758 1106 56-57 1.2352865358868712 1106 58-59 1.3517675050887092 1106 60-61 1.2396318333627434 1106 62-63 1.2801459947040357 1106 64-65 1.305005543828635 1106 66-67 1.3217960567835192 1106 68-69 1.2615133741486915 1106 70-71 1.2749245645719895 1106 72-73 1.300159471170467 1106 74-75 1.2772218006618878 1106 76-77 1.2323079926412746 1106 78-79 1.263999725542078 1106 80-81 1.3576674407576554 1106 82-83 1.3980340897158108 1106 84-85 1.4791253031360334 1106 86-87 1.4128900806639564 1106 88-89 1.4816730082298157 1106 90-91 1.511551533456938 1106 92-93 1.5491282890979008 1106 94-95 1.6414516470144065 1106 96-97 1.5949364199168414 1106 98-99 1.8949832487834826 1106 100-101 1.9336964875944425 1107 1 0.22605894371231017 1107 2 0.38069543087669544 1107 3 0.39365942266404375 1107 4 0.4239964833570511 1107 5 0.18377393001963327 1107 6 0.27479057446518595 1107 7 0.41172625686783704 1107 8 0.38414551139617004 1107 9 0.4969839494222654 1107 10-11 0.5119549094668798 1107 12-13 0.47463357604269163 1107 14-15 0.3809504854735124 1107 16-17 0.4703517893757123 1107 18-19 0.5340007053876334 1107 20-21 0.620463422215856 1107 22-23 0.5501608110000404 1107 24-25 0.5732724542540879 1107 26-27 0.5565162378685429 1107 28-29 0.571921149525572 1107 30-31 0.6432205513499909 1107 32-33 0.6993087279991954 1107 34-35 0.8931796985365708 1107 36-37 0.8471900452249983 1107 38-39 0.7994928912673842 1107 40-41 0.832706659436333 1107 42-43 0.841619600790267 1107 44-45 0.8410178633595606 1107 46-47 0.822737618768862 1107 48-49 0.9494490401492826 1107 50-51 0.8418839713531412 1107 52-53 0.6324632314406458 1107 54-55 0.8357002430633216 1107 56-57 0.8791255410643899 1107 58-59 0.871941370166688 1107 60-61 0.7596100332947415 1107 62-63 0.8601993542580857 1107 64-65 0.9252429679877636 1107 66-67 0.843143545979764 1107 68-69 0.9396459511301742 1107 70-71 1.0243488469283477 1107 72-73 1.0098077983709999 1107 74-75 1.0645387382217812 1107 76-77 0.974864373360532 1107 78-79 1.0951019199624454 1107 80-81 1.088994072452767 1107 82-83 1.2197113489514102 1107 84-85 1.1768077989357444 1107 86-87 1.1297949808013783 1107 88-89 1.259272246401057 1107 90-91 1.1700599232189752 1107 92-93 1.1945098764758697 1107 94-95 1.2974775466981505 1107 96-97 1.257320795018849 1107 98-99 1.2073156176667474 1107 100-101 1.4788001461109026 1108 1 0.09439840553596568 1108 2 0.2458904663201018 1108 3 0.12616545113449718 1108 4 0.12224578842560163 1108 5 0.1493345256950107 1108 6 0.17365428259364535 1108 7 0.1087956580943299 1108 8 0.060242812049949634 1108 9 0.08063924754926433 1108 10-11 0.11323271705774118 1108 12-13 -0.08290645091803839 1108 14-15 0.3001755116687974 1108 16-17 0.2495078299642728 1108 18-19 0.3269355165682981 1108 20-21 0.2839651625616213 1108 22-23 0.1818506111884446 1108 24-25 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-2.014157934446274 2316 68-69 -1.4535990582481517 2316 70-71 -1.9153274427875928 2316 72-73 -1.7634343514423918 2316 74-75 -1.9375715308902244 2316 76-77 -2.2252547606925077 2316 78-79 -2.519753577848121 2316 80-81 -2.3778945122914656 2316 82-83 -2.53480926975762 2316 84-85 -2.078584306231349 2316 86-87 -2.0867612193554166 2316 88-89 -2.522225846932592 2316 90-91 -2.5687532964889037 2316 92-93 -2.823698627818114 2316 94-95 -2.91532978410223 2316 96-97 -2.9229115424596444 2316 98-99 -3.036476707226189 2316 100-101 -2.68536184201394 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8201.0 3 2625.0 4 2756.0 5 2837.0 6 3174.0 7 3646.0 8 4395.0 9 5424.0 10 6526.0 11 8043.0 12 9470.0 13 11194.0 14 12362.0 15 14220.0 16 17148.0 17 21284.0 18 19336.0 19 15096.0 20 14966.0 21 16175.0 22 17655.0 23 19644.0 24 22412.0 25 26025.0 26 30025.0 27 35488.0 28 41843.0 29 50636.0 30 62157.0 31 76813.0 32 97209.0 33 124775.0 34 168573.0 35 237572.0 36 352044.0 37 500416.0 38 429976.0 39 72274.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43482702138734 18.872823867170155 14.31126551844421 23.381083592998294 2 15.339240177259967 20.1116598819542 40.30173852320575 24.247361417580088 3 19.127069076594513 24.37513584427534 29.544769005223838 26.95302607390631 4 12.890849364149256 16.688109635982332 37.029788872328886 33.39125212753953 5 14.169682524006172 36.8605364250897 34.020783554283675 14.948997496620455 6 31.945307013725945 35.94048070275834 17.616932841125603 14.49727944239011 7 28.345591679940036 32.44698460289203 20.88689528092695 18.32052843624098 8 26.051782714322293 35.06032156931321 19.921965263769277 18.965930452595224 9 26.11395662353695 16.960282164473874 20.4370129264218 36.488748285567375 10-11 24.807767155539473 26.71016670394795 27.42614304223872 21.05592309827386 12-13 25.61874828628275 23.97882943231927 27.95633180103023 22.446090480367754 14-15 22.660897835361133 25.395029564642417 26.01290395106655 25.931168648929898 16-17 22.275416865601663 28.659795585263183 26.566830252069344 22.497957297065806 18-19 22.223627328377994 27.226995680079185 28.806686275703687 21.742690715839135 20-21 22.793672449288806 26.522252984563956 28.862742741250507 21.821331824896735 22-23 22.64388736974009 26.368843691231085 28.791074091317252 22.196194847711567 24-25 22.451987751474732 26.64639553293669 28.7570322201769 22.14458449541168 26-27 22.26909248395913 26.762115267939528 28.668154515145428 22.30063773295591 28-29 22.1367939428148 26.750330753575756 28.860890733378376 22.251984570231066 30-31 22.250689575798717 26.63100769749958 28.759543846096825 22.358758880604874 32-33 22.117259757910077 26.82588162910432 28.68604252169752 22.37081609128808 34-35 22.50999513117732 26.807004238372567 28.4942970793306 22.188703551119517 36-37 22.517687193200253 26.61775297153429 28.45186048126644 22.41269935399902 38-39 22.251138797415106 26.81159547512495 28.605834177519164 22.331431549940785 40-41 22.47704077153153 26.654908211862903 28.61768567855324 22.250365338052326 42-43 22.356413301500506 26.77174464206315 28.406093656320607 22.465748400115736 44-45 22.465154141873377 26.91896307980599 28.200746007925137 22.415136770395492 46-47 22.483715381776676 27.01885184956906 27.931699397143394 22.56573337151087 48-49 22.631042930400756 27.148641365358518 27.686083331203875 22.534232373036843 50-51 22.621135829166526 27.238520848472508 27.645859735469607 22.494483586891356 52-53 22.694447792962492 27.26892006976881 27.51886906176085 22.517763075507848 54-55 22.62855965742394 27.16103665463988 27.565048408844056 22.645355279092126 56-57 22.48634940452386 27.572017986207975 27.450718375388938 22.490914233879227 58-59 22.597056145035815 27.219108993022743 27.558431900138046 22.625402961803395 60-61 22.624181837938607 27.097285567944102 27.849552796923742 22.428979797193545 62-63 22.504173173227073 27.226180135929557 27.81631638545059 22.453330305392775 64-65 22.498652633377592 27.242182884123352 27.687716015102993 22.571448467396067 66-67 22.34342392992435 27.537093458658706 27.459441467568002 22.660041143848947 68-69 22.29289012450422 27.358251864851635 27.719390379770935 22.629467630873208 70-71 22.39021136676561 27.30178758790697 27.519765659433887 22.78823538589354 72-73 22.465057892808257 27.2665931984214 27.45384556730428 22.814503341466065 74-75 22.456699719581366 27.463473220195915 27.33800840748818 22.74181865273454 76-77 22.51540851961369 27.410594304509452 27.334051555559718 22.739945620317144 78-79 22.44491710935104 27.45796156820695 27.341213076143273 22.755908246298738 80-81 22.374815770727892 27.5958479852008 27.30375340078069 22.72558284329062 82-83 22.48255594135878 27.551194795597343 27.320109547478705 22.646139715565177 84-85 22.48797564088132 27.447905499355496 27.247871825492133 22.816247034271058 86-87 22.47205742823568 27.398324001745806 27.410674201914652 22.71894436810386 88-89 22.572429920676846 27.407908584881746 27.166564960309152 22.853096534132256 90-91 22.437510889026456 27.456832631200452 27.40920868749699 22.696447792276103 92-93 22.51617237499032 27.31700625743545 27.33218691407885 22.834634453495386 94-95 22.432924445956985 27.343475738488227 27.31927852495347 22.904321290601317 96-97 22.455695328037724 27.325037796726505 27.324959734557403 22.89430714067837 98-99 22.28712130425811 27.291940256317105 27.22654228427893 23.194396155145856 100-101 22.37899116947288 27.29850211334527 27.26945484047035 23.053051876711507 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 507.0 1 386.5 2 340.5 3 429.5 4 393.5 5 408.5 6 632.0 7 1220.5 8 2067.5 9 2559.0 10 2402.0 11 2153.5 12 2223.0 13 2429.0 14 2642.5 15 2791.0 16 2906.5 17 3061.0 18 3269.5 19 3525.0 20 3963.5 21 4667.0 22 5800.0 23 7234.5 24 8774.0 25 10971.0 26 13871.5 27 17293.0 28 20581.5 29 24098.5 30 28894.5 31 34092.0 32 38522.0 33 42860.0 34 48663.5 35 53566.5 36 58371.5 37 64235.5 38 68763.0 39 71410.0 40 73711.0 41 78474.5 42 82583.0 43 87147.0 44 92784.0 45 95794.5 46 96136.5 47 96701.0 48 100638.5 49 101310.5 50 98511.0 51 96224.5 52 92314.0 53 89740.5 54 86817.0 55 80950.0 56 74467.0 57 67006.0 58 60259.0 59 53855.0 60 45605.5 61 36526.0 62 28757.0 63 22548.0 64 17215.0 65 12870.5 66 9510.0 67 7306.0 68 5275.0 69 3431.5 70 2324.5 71 1581.5 72 1041.5 73 738.5 74 526.5 75 356.0 76 223.0 77 123.5 78 75.5 79 56.0 80 39.0 81 29.0 82 22.5 83 21.5 84 18.5 85 13.0 86 10.5 87 9.0 88 7.0 89 6.0 90 6.0 91 5.0 92 5.0 93 3.5 94 3.0 95 2.5 96 1.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05962299084363998 2 0.09982659029412579 3 0.06902072844554791 4 0.1321922426160494 5 0.10567580456917222 6 0.1035700874301555 7 0.11413766788707273 8 0.0979158469642773 9 0.12103974073162752 10-11 0.11074512360754579 12-13 0.0935679310198261 14-15 0.0982083076780296 16-17 0.11269486169922793 18-19 0.09214462221289814 20-21 0.0768391781931933 22-23 0.07697565985961106 24-25 0.06588165011793966 26-27 0.0570103418007859 28-29 0.06779239344778816 30-31 0.04979631086156197 32-33 0.051024645859321725 34-35 0.04675471943853782 36-37 0.05042022705090025 38-39 0.05172655157232729 40-41 0.06831882273254233 42-43 0.0656476815469378 44-45 0.052155493952497356 46-47 0.03840984040613824 48-49 0.046540248248452785 50-51 0.028622155185893877 52-53 0.04383011230101461 54-55 0.049874300385229255 56-57 0.05350081323575804 58-59 0.06040288608031283 60-61 0.056990844419869076 62-63 0.06430236226367712 64-65 0.043420667301761355 66-67 0.06749993273403584 68-69 0.05621094918319622 70-71 0.025619558524703375 72-73 0.020569736867246623 74-75 0.03041591423024145 76-77 0.04576035301177993 78-79 0.06523823654768454 80-81 0.04152942135282967 82-83 0.06005193322381004 84-85 0.06350296964608744 86-87 0.06767540916228723 88-89 0.09095528197697203 90-91 0.06475080202476402 92-93 0.07703415200236152 94-95 0.0845601410362546 96-97 0.09306099911598875 98-99 0.12499770905774227 100-101 0.12107873549346117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2564447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.090642890576525 #Duplication Level Percentage of deduplicated Percentage of total 1 77.71686562705162 45.14622687715977 2 12.86472880091371 14.946407333159861 3 4.0248703844008284 7.014219245432581 4 1.7500180083875085 4.0663868470926685 5 0.9641904761627429 2.800522231463242 6 0.5649939312455069 1.9692516417195414 7 0.38928657718626064 1.5829735276195345 8 0.28296704838116526 1.3150190205848609 9 0.20561962357545072 1.075011851197465 >10 1.1150121628103555 11.809983260852533 >50 0.08246667922900364 3.26938279791277 >100 0.037904269726906074 3.9013231328145173 >500 7.400325137310869E-4 0.25832459566278576 >1k 2.0182704919573285E-4 0.2632878670125619 >5k 1.3455136613048856E-4 0.5816797703154181 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8566 0.33402912986698496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6318 0.24636890526495578 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4037 0.15742185352241633 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.798952366728578E-5 2 0.0 0.0 0.0 0.0 7.798952366728578E-5 3 0.0 0.0 0.0 3.899476183364289E-5 7.798952366728578E-5 4 0.0 0.0 0.0 3.899476183364289E-5 7.798952366728578E-5 5 3.899476183364289E-5 0.0 0.0 3.899476183364289E-5 7.798952366728578E-5 6 3.899476183364289E-5 0.0 0.0 3.899476183364289E-5 7.798952366728578E-5 7 1.5597904733457156E-4 0.0 0.0 3.899476183364289E-5 7.798952366728578E-5 8 1.9497380916821443E-4 0.0 0.0 3.899476183364289E-5 7.798952366728578E-5 9 1.9497380916821443E-4 0.0 0.0 7.798952366728578E-5 7.798952366728578E-5 10-11 1.9497380916821443E-4 0.0 0.0 7.798952366728578E-5 7.798952366728578E-5 12-13 1.9497380916821443E-4 0.0 0.0 9.748690458410723E-5 7.798952366728578E-5 14-15 2.3396857100185732E-4 0.0 0.0 1.1698428550092866E-4 7.798952366728578E-5 16-17 2.3396857100185732E-4 0.0 0.0 1.1698428550092866E-4 7.798952366728578E-5 18-19 2.7296333283550024E-4 0.0 0.0 1.1698428550092866E-4 7.798952366728578E-5 20-21 3.119580946691431E-4 0.0 0.0 2.1447119008503586E-4 7.798952366728578E-5 22-23 3.119580946691431E-4 0.0 0.0 3.119580946691431E-4 1.1698428550092866E-4 24-25 3.50952856502786E-4 1.9497380916821445E-5 0.0 3.314554755859645E-4 2.3396857100185732E-4 26-27 3.50952856502786E-4 3.899476183364289E-5 0.0 5.654240465878218E-4 2.3396857100185732E-4 28-29 3.899476183364289E-4 3.899476183364289E-5 0.0 0.001540293092428894 2.3396857100185732E-4 30-31 4.289423801700718E-4 3.899476183364289E-5 0.0 0.0037045023741960744 2.3396857100185732E-4 32-33 4.289423801700718E-4 3.899476183364289E-5 0.0 0.00662910951171929 2.3396857100185732E-4 34-35 5.069319038373576E-4 3.899476183364289E-5 0.0 0.009592711411076149 2.3396857100185732E-4 36-37 6.434135702551077E-4 3.899476183364289E-5 0.0 0.012380836882181617 2.3396857100185732E-4 38-39 7.01905713005572E-4 3.899476183364289E-5 0.0 0.01538343354337212 2.3396857100185732E-4 40-41 7.01905713005572E-4 3.899476183364289E-5 0.0 0.01969235472598966 2.3396857100185732E-4 42-43 7.409004748392149E-4 3.899476183364289E-5 0.0 0.025736542810204308 2.3396857100185732E-4 44-45 8.968795221737864E-4 3.899476183364289E-5 0.0 0.031273798990581594 2.3396857100185732E-4 46-47 0.0010333611885915365 3.899476183364289E-5 0.0 0.03710351588471121 2.7296333283550024E-4 48-49 0.0010528585695083578 3.899476183364289E-5 0.0 0.043011222302508104 3.119580946691431E-4 50-51 0.0010528585695083578 3.899476183364289E-5 0.0 0.051414593477658144 3.119580946691431E-4 52-53 0.0010528585695083578 3.899476183364289E-5 0.0 0.0718283512975702 3.119580946691431E-4 54-55 0.0010528585695083578 3.899476183364289E-5 0.0 0.10298516600265087 3.119580946691431E-4 56-57 0.0011308480931756437 3.899476183364289E-5 0.0 0.1279418135761823 3.119580946691431E-4 58-59 0.0011308480931756437 3.899476183364289E-5 0.0 0.16231569613253852 3.119580946691431E-4 60-61 0.0011308480931756437 3.899476183364289E-5 0.0 0.20337718034336447 3.119580946691431E-4 62-63 0.0011308480931756437 3.899476183364289E-5 0.0 0.2296011576764893 3.119580946691431E-4 64-65 0.0011308480931756437 3.899476183364289E-5 0.0 0.25134073739874524 3.119580946691431E-4 66-67 0.0011308480931756437 3.899476183364289E-5 0.0 0.3128549741913169 3.119580946691431E-4 68-69 0.0012088376168429295 3.899476183364289E-5 0.0 0.3817977131131975 3.119580946691431E-4 70-71 0.0012673297595933938 3.899476183364289E-5 0.0 0.4114922242495166 4.289423801700717E-4 72-73 0.0013063245214270367 3.899476183364289E-5 0.0 0.47014034604731547 6.239161893382862E-4 74-75 0.001325821902343858 3.899476183364289E-5 0.0 0.5544665185125682 6.239161893382862E-4 76-77 0.001325821902343858 3.899476183364289E-5 0.0 0.6383442512167341 6.434135702551077E-4 78-79 0.001325821902343858 3.899476183364289E-5 0.0 0.713916099650334 6.62910951171929E-4 80-81 0.001364816664177501 3.899476183364289E-5 0.0 0.8197478832668408 7.01905713005572E-4 82-83 0.001364816664177501 3.899476183364289E-5 0.0 0.945077047800169 7.798952366728577E-4 84-85 0.0014233088069279653 3.899476183364289E-5 0.0 1.0804473635056602 7.798952366728577E-4 86-87 0.0014818009496784298 3.899476183364289E-5 0.0 1.2831031407551023 7.993926175896792E-4 88-89 0.0014818009496784298 3.899476183364289E-5 0.0 1.5269178891199546 8.383873794233221E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4800 0.0 47.036877 1 GTATCAA 8455 0.0 41.151302 1 ATCAACG 10400 0.0 32.9499 3 TCAACGC 10540 0.0 32.51287 4 TATCAAC 10620 0.0 32.404118 2 CAACGCA 10825 0.0 31.656256 5 AACGCAG 11145 0.0 30.9161 6 ACGCAGA 12745 0.0 26.811172 7 CGCAGAG 12840 0.0 26.503313 8 GCAGAGT 13970 0.0 23.305763 9 GTACATG 8690 0.0 23.19168 1 TAGTGCG 195 4.199901E-7 21.93699 9 TACATGG 9000 0.0 21.706282 2 GAGTACT 7685 0.0 20.987743 12-13 ACATGGG 9040 0.0 20.76734 3 GTGGTAT 2630 0.0 20.061037 1 AGAGTAC 12795 0.0 19.887014 10-11 ATTCGCG 315 0.0 19.600494 80-81 CAGAGTA 13545 0.0 19.50501 10-11 GTACTTT 8350 0.0 19.317396 14-15 >>END_MODULE