FastQCFastQC Report
Thu 26 May 2016
SRR936811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564447
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96570.37657241502748934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79150.30864353991328347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51980.2026947720112757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.051.48731
GTATCAA93500.039.3798871
ATCAACG118650.030.8652173
TATCAAC121700.030.2478052
TCAACGC121050.030.2122634
CAACGCA123400.029.636915
AACGCAG125950.029.0745856
ACGCAGA142700.025.4288527
CGCAGAG145250.024.7535258
GCAGAGT154000.022.884459
GAGTACT94500.022.8433312-13
GTACATG85300.021.3321251
GTACTTT101650.020.9100814-15
AGAGTAC140750.020.3988310-11
CAGAGTA150000.020.01166210-11
TACATGG88150.019.9909922
AGTACTT99800.019.9883112-13
TATACCG1707.963837E-519.5571925
ACATGGG85850.019.4753423
GTGGTAT27700.018.1806951
Produced by FastQC (version 0.11.5)