##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936811_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2564447 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1230191148423 31.0 31.0 34.0 31.0 34.0 2 32.11765265571876 33.0 31.0 34.0 30.0 34.0 3 32.30322638759935 33.0 31.0 34.0 30.0 34.0 4 36.03845897380605 37.0 35.0 37.0 35.0 37.0 5 35.960713557347844 37.0 35.0 37.0 35.0 37.0 6 36.00075766822243 37.0 35.0 37.0 35.0 37.0 7 35.939809245424065 37.0 35.0 37.0 35.0 37.0 8 35.9171743459701 37.0 35.0 37.0 35.0 37.0 9 37.607405417230304 39.0 37.0 39.0 35.0 39.0 10-11 37.53075419378915 39.0 37.0 39.0 35.0 39.0 12-13 37.43440359656488 39.0 37.0 39.0 34.0 39.0 14-15 38.65599269550121 40.0 38.0 41.0 34.0 41.0 16-17 38.56215823528426 40.0 38.0 41.0 34.0 41.0 18-19 38.60339539089714 40.0 38.0 41.0 34.0 41.0 20-21 38.684936362498426 40.0 38.0 41.0 34.0 41.0 22-23 38.61777568419234 40.0 38.0 41.0 34.0 41.0 24-25 38.52091386564042 40.0 38.0 41.0 34.0 41.0 26-27 38.37081464346895 40.0 38.0 41.0 34.0 41.0 28-29 38.19496172079205 40.0 38.0 41.0 34.0 41.0 30-31 38.075617862252564 40.0 37.0 41.0 33.5 41.0 32-33 38.078161100619354 40.0 37.0 41.0 33.5 41.0 34-35 38.003629827405284 40.0 37.0 41.0 33.0 41.0 36-37 37.857152243739094 40.0 37.0 41.0 33.0 41.0 38-39 37.70438831451771 40.0 37.0 41.0 33.0 41.0 40-41 37.537241362367794 40.0 37.0 41.0 33.0 41.0 42-43 37.330569319623294 40.0 37.0 41.0 32.0 41.0 44-45 37.02046835048648 40.0 36.0 41.0 31.0 41.0 46-47 36.779730093856486 39.0 36.0 41.0 31.0 41.0 48-49 36.485163857938964 39.0 36.0 41.0 30.0 41.0 50-51 36.22833109828357 39.0 35.0 40.5 30.0 41.0 52-53 35.967456726537925 39.0 35.0 40.0 29.0 41.0 54-55 35.72927964586518 38.5 35.0 40.0 28.5 41.0 56-57 35.45586163410669 38.0 35.0 40.0 28.0 41.0 58-59 35.12424803476149 38.0 34.0 40.0 27.5 41.0 60-61 34.79633191873336 38.0 34.0 40.0 27.0 41.0 62-63 34.41732447580316 37.0 34.0 40.0 26.0 41.0 64-65 34.014574877156754 36.5 33.0 39.0 26.0 41.0 66-67 33.63335760107345 36.0 33.0 39.0 25.5 40.0 68-69 33.190373207167085 35.5 32.0 39.0 25.0 40.0 70-71 32.717123418811155 35.0 32.0 38.0 23.5 40.0 72-73 32.362908455507174 35.0 31.5 37.5 22.5 39.5 74-75 31.91100459475279 35.0 31.0 37.0 22.0 39.0 76-77 30.5283330090269 33.5 29.5 35.5 20.5 38.0 78-79 31.25375412320863 34.0 31.0 36.0 21.0 38.0 80-81 31.088618520874093 34.0 31.0 36.0 20.0 37.0 82-83 30.757406762549586 34.0 31.0 35.0 19.5 37.0 84-85 30.378014051372478 34.0 30.5 35.0 18.0 36.5 86-87 30.013518704032485 34.0 30.0 35.0 16.0 36.0 88-89 29.650165513266604 34.0 30.0 35.0 9.0 36.0 90-91 29.223390656933056 34.0 29.0 35.0 4.5 35.0 92-93 28.754272753541017 34.0 29.0 35.0 2.0 35.0 94-95 28.26230216495018 33.5 29.0 35.0 2.0 35.0 96-97 27.61893187888071 33.0 27.0 35.0 2.0 35.0 98-99 26.845970105835683 33.0 26.5 35.0 2.0 35.0 100-101 23.715053576853023 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3378010479456819 1101 2 0.25687192400300773 1101 3 0.23426702297971502 1101 4 0.27519210651159653 1101 5 0.31895267702818586 1101 6 0.28827595976962783 1101 7 0.3404842359426965 1101 8 0.3108285440862062 1101 9 0.4473828953010397 1101 10-11 0.4003660501313675 1101 12-13 0.362588340821155 1101 14-15 0.4810026851919744 1101 16-17 0.5504026002032703 1101 18-19 0.5194784080878208 1101 20-21 0.4874724128574144 1101 22-23 0.4012781414102804 1101 24-25 0.47844893859237203 1101 26-27 0.4666649327105148 1101 28-29 0.5648888793720559 1101 30-31 0.5095795007410544 1101 32-33 0.5903980523562993 1101 34-35 0.5944763115810616 1101 36-37 0.62092633439579 1101 38-39 0.5187722619169506 1101 40-41 0.5727860040102328 1101 42-43 0.73167200488448 1101 44-45 0.6902349209533654 1101 46-47 0.7545521711349039 1101 48-49 0.5658839544139624 1101 50-51 0.5717295091240331 1101 52-53 0.5234623123643303 1101 54-55 0.6178975370588304 1101 56-57 0.6956825189047535 1101 58-59 0.6502779935663838 1101 60-61 0.6735936892877419 1101 62-63 0.7516905697135527 1101 64-65 0.5369233020325197 1101 66-67 0.6000053415876181 1101 68-69 0.5770279972798775 1101 70-71 0.5190433182681815 1101 72-73 0.6391385490413555 1101 74-75 0.5763433544481025 1101 76-77 0.38023664584545 1101 78-79 0.5844876752341825 1101 80-81 0.8116918066993648 1101 82-83 0.8587082935099737 1101 84-85 0.8548750070919056 1101 86-87 0.6884946816910968 1101 88-89 0.7701818563966469 1101 90-91 0.9117415984338848 1101 92-93 0.9795640474131098 1101 94-95 1.1076835759473873 1101 96-97 1.059700167048078 1101 98-99 0.9943726346536863 1101 100-101 1.096519329010416 1102 1 0.2253591207529908 1102 2 0.1168610789818203 1102 3 0.1552158606440983 1102 4 0.18980377999785958 1102 5 0.20472486672206713 1102 6 0.20403204958157062 1102 7 0.24032856893816756 1102 8 0.22089388355240658 1102 9 0.34604172401676436 1102 10-11 0.31702346399232795 1102 12-13 0.2987180781660612 1102 14-15 0.38476968103865516 1102 16-17 0.38040826731774047 1102 18-19 0.36413055664427674 1102 20-21 0.2860596056184548 1102 22-23 0.20867801322685864 1102 24-25 0.2572256911104134 1102 26-27 0.3382770316426118 1102 28-29 0.3112342501475496 1102 30-31 0.17635591850907417 1102 32-33 0.24240939702900022 1102 34-35 0.24849986665925883 1102 36-37 0.30747327250496426 1102 38-39 0.32555787569127403 1102 40-41 0.28867049711405457 1102 42-43 0.2864973522219856 1102 44-45 0.3712527121748934 1102 46-47 0.3407798462218068 1102 48-49 0.25409035116254586 1102 50-51 0.17579739521888627 1102 52-53 0.2717111272723045 1102 54-55 0.26734122814777095 1102 56-57 0.3513680061446749 1102 58-59 0.3464950297589624 1102 60-61 0.3528524625965659 1102 62-63 0.39756984643364746 1102 64-65 0.32392465367504997 1102 66-67 0.26171090118631213 1102 68-69 0.04280228122264873 1102 70-71 0.026695984065632672 1102 72-73 0.16126784774831293 1102 74-75 -0.06163672875019799 1102 76-77 0.026234239383072122 1102 78-79 0.08927318512057525 1102 80-81 0.1122596724563607 1102 82-83 0.27717151608080925 1102 84-85 0.21438718451711125 1102 86-87 0.15310613530870754 1102 88-89 0.3377548445435288 1102 90-91 0.4599346079300126 1102 92-93 0.46914275434358643 1102 94-95 0.4146940741257765 1102 96-97 0.6014646981671881 1102 98-99 0.8250688267485309 1102 100-101 0.7844786483630308 1103 1 0.032579876306556343 1103 2 -0.1861238233705862 1103 3 -0.03795058771719084 1103 4 0.06673471481946791 1103 5 0.06787078285211834 1103 6 0.10632166285562761 1103 7 0.12580570178851502 1103 8 0.1593594780820311 1103 9 0.033574266433767264 1103 10-11 0.1347183015446518 1103 12-13 0.20749469176146818 1103 14-15 0.15213067915525613 1103 16-17 0.32808743366236826 1103 18-19 0.36920145335057697 1103 20-21 0.25767232136450957 1103 22-23 0.2943075361143457 1103 24-25 0.27918915309476944 1103 26-27 0.3313261747600933 1103 28-29 0.24368000600620832 1103 30-31 0.2620848483110194 1103 32-33 0.3299938801943938 1103 34-35 0.3182961053660165 1103 36-37 0.36483347740016114 1103 38-39 0.41605401534705777 1103 40-41 0.2950419464312759 1103 42-43 0.21492335686740915 1103 44-45 0.3810094626925249 1103 46-47 0.17132715882833338 1103 48-49 0.1289740374455164 1103 50-51 -0.06696534920516939 1103 52-53 0.0900104147967653 1103 54-55 0.10303342061176579 1103 56-57 0.14713319531781366 1103 58-59 0.12047434193430462 1103 60-61 0.2528508863232375 1103 62-63 0.13873203784000765 1103 64-65 -0.02529247238207688 1103 66-67 0.026002361242532857 1103 68-69 0.19886834029527023 1103 70-71 0.08750733770814634 1103 72-73 0.3142801314937813 1103 74-75 0.15559687088857643 1103 76-77 0.13891769725894676 1103 78-79 0.12695310909335333 1103 80-81 0.1461419217882245 1103 82-83 0.3374260893073462 1103 84-85 0.239461116820749 1103 86-87 0.12067583709813334 1103 88-89 0.35200804183599743 1103 90-91 0.42161489495413207 1103 92-93 0.39538549331679107 1103 94-95 0.4457839044170697 1103 96-97 0.5842071747671547 1103 98-99 0.6252807592392848 1103 100-101 0.6796133790541923 1104 1 0.2510561903247108 1104 2 0.014408913081467745 1104 3 0.09248497174542081 1104 4 0.14500569902268268 1104 5 0.10821540368420557 1104 6 0.09279981570919915 1104 7 0.19752947215666694 1104 8 0.21821612268726653 1104 9 0.2142168735572909 1104 10-11 0.23697525250333484 1104 12-13 0.18883966305455857 1104 14-15 0.2708398340490632 1104 16-17 0.23037569127902913 1104 18-19 0.2695407343107945 1104 20-21 0.17898789273208138 1104 22-23 0.24552549656407052 1104 24-25 0.27250805958498603 1104 26-27 0.2582683936198009 1104 28-29 0.3588029979067997 1104 30-31 0.3336481289833131 1104 32-33 0.4948328787182774 1104 34-35 0.3956512453883363 1104 36-37 0.33076192975367746 1104 38-39 0.3171047474480915 1104 40-41 0.5291410230061047 1104 42-43 0.46084494038957047 1104 44-45 0.5260232935222149 1104 46-47 0.5370268926260238 1104 48-49 0.5973585245561779 1104 50-51 0.5534638212998502 1104 52-53 0.590848789995249 1104 54-55 0.6791190648052776 1104 56-57 0.689329797045815 1104 58-59 0.5017208370052586 1104 60-61 0.5762628934284351 1104 62-63 0.5792390499953584 1104 64-65 0.5281822475873383 1104 66-67 0.6181290622099667 1104 68-69 0.5541814872793296 1104 70-71 0.5460664095301908 1104 72-73 0.5491700425818493 1104 74-75 0.3936262539674651 1104 76-77 0.36860250953513685 1104 78-79 0.39905001959970576 1104 80-81 0.7197907389376859 1104 82-83 0.6707829696378056 1104 84-85 0.7531172903328915 1104 86-87 0.6748999769435571 1104 88-89 0.8749521337348902 1104 90-91 1.0400375932894015 1104 92-93 0.9715900849429424 1104 94-95 0.9980203969278492 1104 96-97 1.1443776014234253 1104 98-99 0.9147779434268308 1104 100-101 0.9693289951419111 1105 1 0.18160989287468254 1105 2 -0.011629317449838084 1105 3 0.07849175354187565 1105 4 0.1717603797148044 1105 5 0.13410634577128633 1105 6 0.13257299966512193 1105 7 0.19023114614491732 1105 8 0.11810078073938968 1105 9 0.19491678334821927 1105 10-11 0.2127699675002006 1105 12-13 0.2844898395354889 1105 14-15 0.39719210312751585 1105 16-17 0.25128391833823827 1105 18-19 0.35959027790021736 1105 20-21 0.3061922723804855 1105 22-23 0.30558429770141515 1105 24-25 0.31188303433991393 1105 26-27 0.38259085657634273 1105 28-29 0.33761222999545026 1105 30-31 0.3860950551632385 1105 32-33 0.48706922730310254 1105 34-35 0.4133882828832469 1105 36-37 0.38759017714767197 1105 38-39 0.45015027671340135 1105 40-41 0.32782587183961454 1105 42-43 0.3006707973043561 1105 44-45 0.46727315633180666 1105 46-47 0.42708259703402973 1105 48-49 0.5662192586401531 1105 50-51 0.5934222378144298 1105 52-53 0.6585474141743006 1105 54-55 0.5440198613460794 1105 56-57 0.5510075878660317 1105 58-59 0.5628502333715204 1105 60-61 0.6021007953555468 1105 62-63 0.48863442073179186 1105 64-65 0.4296258783413265 1105 66-67 0.46420712998141056 1105 68-69 0.27237993336584054 1105 70-71 0.23557318908263625 1105 72-73 0.3639327919063007 1105 74-75 0.3446646782993561 1105 76-77 0.4399043965816283 1105 78-79 0.6665656144267409 1105 80-81 0.7596257350800357 1105 82-83 0.6567046831776935 1105 84-85 0.6863225928800816 1105 86-87 0.6381300900455784 1105 88-89 0.6277417401483412 1105 90-91 0.7992411830474282 1105 92-93 0.8199617316318317 1105 94-95 0.8400374244512037 1105 96-97 1.1785993166361202 1105 98-99 1.0867053779599765 1105 100-101 0.8861377482371076 1106 1 0.34789522851480115 1106 2 0.20427168283918462 1106 3 0.19111565675361675 1106 4 0.27125420977208137 1106 5 0.15610035122069377 1106 6 0.2690470438115753 1106 7 0.20551711588213806 1106 8 0.21530662463737116 1106 9 0.33242757122309996 1106 10-11 0.33753218108652305 1106 12-13 0.35423699625280847 1106 14-15 0.5216073927561453 1106 16-17 0.4750546544715988 1106 18-19 0.4597913830216953 1106 20-21 0.4348736189489628 1106 22-23 0.5167379616849956 1106 24-25 0.5285402348666608 1106 26-27 0.43576798853064247 1106 28-29 0.5590578874708925 1106 30-31 0.5675242006024206 1106 32-33 0.6043546169874858 1106 34-35 0.6739231399294212 1106 36-37 0.46788452710414674 1106 38-39 0.5587508187002683 1106 40-41 0.4839722834844409 1106 42-43 0.501852789215171 1106 44-45 0.5691094364207814 1106 46-47 0.37955493794665074 1106 48-49 0.39533512452844377 1106 50-51 0.35402901402648723 1106 52-53 0.40026546486112125 1106 54-55 0.46380817227453264 1106 56-57 0.5486778097060281 1106 58-59 0.47705301822932 1106 60-61 0.4860349264629278 1106 62-63 0.59596265644808 1106 64-65 0.7178894760988612 1106 66-67 0.7020486788835925 1106 68-69 0.7004444870692339 1106 70-71 0.6669001203514071 1106 72-73 0.7682041022181139 1106 74-75 0.7640922185228938 1106 76-77 0.5908585297769307 1106 78-79 0.5467781594790218 1106 80-81 0.6683483130657528 1106 82-83 0.7084907625140175 1106 84-85 0.6158250263829714 1106 86-87 0.5315639289446352 1106 88-89 0.6800988929080063 1106 90-91 0.9371120702697837 1106 92-93 1.0387916965970412 1106 94-95 0.9319350129017074 1106 96-97 1.1617522730089647 1106 98-99 1.2047656819707662 1106 100-101 1.3022293133357898 1107 1 0.13578742527141685 1107 2 0.017511960005187177 1107 3 0.07334394351546791 1107 4 0.10298328451249716 1107 5 0.11180300914924146 1107 6 0.19393198280329216 1107 7 0.16967738599173288 1107 8 0.1164618008515319 1107 9 0.19851485878338337 1107 10-11 0.17043359629384014 1107 12-13 0.14396866543604148 1107 14-15 0.09605788910746327 1107 16-17 0.16266149552658504 1107 18-19 0.21953952328591697 1107 20-21 0.17653575310373526 1107 22-23 0.19953285216569583 1107 24-25 0.20812700548219 1107 26-27 0.1270220499119148 1107 28-29 0.29377051643659513 1107 30-31 0.3158130016243632 1107 32-33 0.26371635984865094 1107 34-35 0.34770313015166465 1107 36-37 0.22707152833682187 1107 38-39 0.3226036176287508 1107 40-41 0.3607183702732115 1107 42-43 0.4607981339067422 1107 44-45 0.46135047900977355 1107 46-47 0.37777533868793967 1107 48-49 0.2991778999734436 1107 50-51 0.31677955985339423 1107 52-53 0.41088841354036987 1107 54-55 0.3221168075440701 1107 56-57 0.38042595583836913 1107 58-59 0.3491064423327046 1107 60-61 0.47488577240752505 1107 62-63 0.4221265523226805 1107 64-65 0.4929755999374805 1107 66-67 0.29969356369788613 1107 68-69 0.5196671297993447 1107 70-71 0.46912621023560774 1107 72-73 0.45516590845497973 1107 74-75 0.5593395543044615 1107 76-77 0.7368724988379967 1107 78-79 0.7192086653440235 1107 80-81 0.5884411980795647 1107 82-83 0.6325801127186885 1107 84-85 0.6990879728323343 1107 86-87 0.5861811862804274 1107 88-89 0.6280906570007545 1107 90-91 0.7664954554931995 1107 92-93 0.8918407537184301 1107 94-95 0.8534274446765551 1107 96-97 0.8403815313848817 1107 98-99 1.0333205619181847 1107 100-101 1.1171588757170277 1108 1 3.4547465577361436E-4 1108 2 -0.06839023081024465 1108 3 0.0019314083307904184 1108 4 0.043593137908160884 1108 5 0.0020243390729746125 1108 6 0.082764607070132 1108 7 0.09133884666540126 1108 8 0.06215332501881221 1108 9 0.12141325942430115 1108 10-11 0.09219234902454332 1108 12-13 0.07217580675568769 1108 14-15 0.017507572168462104 1108 16-17 0.09979173198017577 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-0.9372849704773571 2316 74-75 -1.0016876031605157 2316 76-77 -1.3477872100368486 2316 78-79 -0.9778730139130367 2316 80-81 -1.0768167393136387 2316 82-83 -1.0630048580801414 2316 84-85 -1.1380698716757074 2316 86-87 -0.9378234417530464 2316 88-89 -0.965131087629377 2316 90-91 -1.1620801740601578 2316 92-93 -1.3813727859594103 2316 94-95 -1.3126538851746936 2316 96-97 -1.191005163496932 2316 98-99 -1.1987894116764686 2316 100-101 -1.3293809493019388 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 16.0 5 53.0 6 108.0 7 255.0 8 635.0 9 1230.0 10 2150.0 11 3400.0 12 4943.0 13 7075.0 14 8967.0 15 12050.0 16 15615.0 17 21330.0 18 19578.0 19 13284.0 20 12116.0 21 12400.0 22 13092.0 23 14552.0 24 17252.0 25 20270.0 26 25032.0 27 31849.0 28 39733.0 29 50058.0 30 62854.0 31 78754.0 32 100868.0 33 130984.0 34 179133.0 35 254819.0 36 386948.0 37 573943.0 38 417216.0 39 31864.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.407213051073334 19.48105688764397 14.958313491436687 23.153416569846012 2 15.743011165289333 20.63328466652316 39.56034528477883 24.06335888340867 3 19.44213285581588 24.307842771764182 29.974176933629803 26.27584743879013 4 13.502331497719194 17.172012980581382 36.53720920619892 32.7884463155005 5 14.10405791879483 36.87530659978209 34.16018761371976 14.860447867703316 6 32.217209215267275 36.10952098703811 17.253123488948855 14.420146308745768 7 28.400625943916953 32.47117214744543 20.783350172571318 18.344851736066296 8 26.271901895418388 35.08081859363832 19.707328714533777 18.93995079640952 9 26.085701907662745 16.742517977560073 20.342280421470985 36.829499693306204 10-11 24.89531843939661 26.698750294410793 27.346025103297244 21.05990616289535 12-13 25.748816939134784 23.84143289625739 28.09160013624868 22.318150028359145 14-15 22.687698361479104 25.292450965061864 26.167766383941643 25.85208428951739 16-17 22.314421003826556 28.532330752010083 26.773472019503618 22.379776224659743 18-19 22.173045494798686 26.940564573960778 29.216142895524843 21.670247035715693 20-21 22.691714059316983 26.194805630340447 29.466185356996906 21.647294953345668 22-23 22.570948044549173 26.084239604093984 29.31300588391961 22.031806467437228 24-25 22.413329854867648 26.32535587004733 29.349573712470495 21.911740562614526 26-27 22.29078292961222 26.47929686311854 29.191048645410962 22.03887156185828 28-29 22.12247027622086 26.42804581726208 29.364761825655595 22.084722080861468 30-31 22.249821863361884 26.315198302056558 29.33318590990869 22.10179392467287 32-33 22.194498788613554 26.40481247784584 29.240115784601333 22.160572948939272 34-35 22.488593266727356 26.39442330118592 29.09590975924242 22.021073672844302 36-37 22.522528670593466 26.24568432427247 29.045075279501358 22.1867117256327 38-39 22.307740152618113 26.44258863126271 29.150812410589793 22.098858805529378 40-41 22.4929436089258 26.292261328637135 29.1791617498758 22.035633312561274 42-43 22.378412104195913 26.419298861332084 28.89348385587272 22.308805178599282 44-45 22.48374882527501 26.553795580304396 28.733363749449197 22.229091844971396 46-47 22.54471392698956 26.719658061999073 28.353503092193762 22.382124918817606 48-49 22.68691386977217 26.85114254145111 28.094378628599305 22.36756496017741 50-51 22.669891800981187 26.94216790325845 28.053501718308198 22.33443857745216 52-53 22.82541026325441 26.96687224267627 27.86563280086086 22.342084693208466 54-55 22.706391295641563 26.9097035705254 27.893083312259286 22.490821821573757 56-57 22.55674882181719 27.32711455323616 27.76732822200901 22.348808402937642 58-59 22.7550178032529 26.94126817809414 27.906964501510867 22.39674951714209 60-61 22.688409599131496 26.830926063383938 28.24448184082207 22.236182496662497 62-63 22.55449225505538 26.92399180018148 28.2029419988013 22.318573945961838 64-65 22.630015151447207 26.937736735269645 27.983091055108883 22.449157058174265 66-67 22.494589961065657 27.219919471870234 27.764832592464924 22.520657974599185 68-69 22.397527575156584 27.066820670039455 28.024259430691856 22.51139232411211 70-71 22.565365495191845 26.93356127084783 27.885014518733193 22.616058715227133 72-73 22.592575044946347 26.947101450546928 27.749126370934235 22.71119713357249 74-75 22.633967592477955 27.123084203131643 27.63553616380681 22.60741204058359 76-77 22.61050654736268 27.09171767053842 27.675726706466293 22.62204907563261 78-79 22.60982463816181 27.092498344180726 27.624680705206455 22.672996312450998 80-81 22.47749756418188 27.25934438714777 27.642328893648195 22.620829155022154 82-83 22.60696884084674 27.251341923986562 27.577849139082407 22.56384009608429 84-85 22.52046259233977 27.242276820331817 27.495944917561765 22.741315669766642 86-87 22.499121629974 27.05910016693905 27.72186495991864 22.719913243168307 88-89 22.63366662494185 27.10748352162145 27.48027722437151 22.778572629065195 90-91 22.48525947822228 27.170543172215268 27.642224271182315 22.701973078380135 92-93 22.49963539883468 26.96440088814504 27.652717195058894 22.883246517961382 94-95 22.439167624801396 26.98077497875271 27.67605226005086 22.904005136395035 96-97 22.528579606428913 26.865590450223365 27.607552752751502 22.998277190596223 98-99 22.29457419358109 26.86783967979947 27.48227358287127 23.35531254374817 100-101 22.171134303232556 27.095416534976952 27.52194498219776 23.211504179592733 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 592.0 1 429.5 2 326.5 3 414.5 4 365.0 5 336.0 6 600.0 7 1357.5 8 2335.0 9 2834.5 10 2628.0 11 2295.0 12 2309.0 13 2507.0 14 2750.5 15 3018.0 16 3236.0 17 3434.0 18 3598.5 19 3841.5 20 4303.0 21 4956.5 22 6009.0 23 7274.0 24 8818.0 25 11036.5 26 13789.5 27 17155.5 28 20523.0 29 24126.0 30 28614.0 31 33629.0 32 38267.0 33 42439.0 34 48340.0 35 53528.0 36 58234.5 37 64018.5 38 68655.5 39 71022.0 40 73059.5 41 77828.0 42 82520.5 43 87722.5 44 92842.0 45 95489.0 46 96306.5 47 97287.5 48 100950.0 49 101363.5 50 98811.0 51 96353.5 52 92270.5 53 89603.0 54 86475.0 55 80484.5 56 74069.5 57 67134.0 58 60738.0 59 54103.5 60 45608.0 61 36754.5 62 28772.0 63 22336.5 64 17070.0 65 12914.5 66 9536.0 67 7241.5 68 5218.5 69 3300.0 70 2159.0 71 1435.0 72 1004.5 73 705.0 74 508.5 75 348.0 76 207.5 77 103.0 78 60.5 79 38.5 80 22.0 81 13.5 82 7.0 83 4.5 84 4.0 85 4.0 86 3.0 87 2.5 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016689758064799155 2 0.002768628090188645 3 0.0028466176138559306 4 3.899476183364288E-5 5 0.0011308480931756437 6 2.729633328355002E-4 7 0.0 8 0.0 9 0.0 10-11 1.1698428550092866E-4 12-13 7.214030939223934E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0021447119008503584 22-23 0.0 24-25 0.003821486659697003 26-27 0.002066722377183073 28-29 0.003353549517693288 30-31 0.015753883780791726 32-33 0.0011698428550092866 34-35 0.0024371726146026804 36-37 0.004035957849782039 38-39 0.002827120232939109 40-41 0.004094449992532503 42-43 0.0018327538061812157 44-45 6.629109511719292E-4 46-47 2.144711900850359E-4 48-49 4.0944499925325026E-4 50-51 2.3396857100185732E-4 52-53 0.0010333611885915365 54-55 0.002983099280273681 56-57 4.0944499925325026E-4 58-59 0.0010918533313420008 60-61 0.0010723559504251792 62-63 0.0 64-65 2.144711900850359E-4 66-67 5.849214275046433E-5 68-69 3.899476183364288E-5 70-71 5.849214275046433E-5 72-73 1.949738091682144E-5 74-75 4.4843976108689324E-4 76-77 6.824083320887505E-4 78-79 2.534659519186788E-4 80-81 0.0044259054681184675 82-83 0.0012478323786765722 84-85 0.002827120232939109 86-87 0.0014818009496784298 88-89 0.0010138638076747151 90-91 9.748690458410721E-5 92-93 3.899476183364288E-5 94-95 9.748690458410721E-5 96-97 0.0012088376168429295 98-99 0.002105717139016716 100-101 8.77382141256965E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2564447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.688258110787025 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9822585108276 42.31319623791512 2 13.700538797253008 15.259182815965545 3 4.362872177702124 7.288822558087422 4 1.956221422640669 4.357542540234583 5 1.015828500025001 2.8284859852842943 6 0.6399950866408323 2.1384126944694106 7 0.4296255139160352 1.6747567556954983 8 0.31212890419531164 1.3905531984532504 9 0.24554591930334582 1.2306622078993685 >10 1.2219320536983034 12.400358982170161 >50 0.08929573533951049 3.4357127887500796 >100 0.04200541991245672 4.15165868732034 >500 0.001401566836582624 0.5062766163939152 >1k 1.4015668365593322E-4 0.13576939400379656 >5k 2.1023502548389982E-4 0.8886085373574154 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9657 0.37657241502748934 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7915 0.30864353991328347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5198 0.2026947720112757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.899476183364289E-5 0.0 0.0 0.0 0.0 3 3.899476183364289E-5 0.0 0.0 0.0 0.0 4 3.899476183364289E-5 0.0 0.0 0.0 7.798952366728578E-5 5 1.5597904733457156E-4 0.0 0.0 0.0 7.798952366728578E-5 6 1.5597904733457156E-4 0.0 0.0 0.0 7.798952366728578E-5 7 2.3396857100185732E-4 0.0 0.0 0.0 7.798952366728578E-5 8 2.3396857100185732E-4 0.0 0.0 0.0 7.798952366728578E-5 9 2.3396857100185732E-4 0.0 0.0 0.0 7.798952366728578E-5 10-11 2.3396857100185732E-4 0.0 0.0 0.0 7.798952366728578E-5 12-13 2.729633328355002E-4 0.0 0.0 0.0 7.798952366728578E-5 14-15 2.729633328355002E-4 0.0 0.0 0.0 7.798952366728578E-5 16-17 2.9246071375232165E-4 0.0 0.0 0.0 7.798952366728578E-5 18-19 3.119580946691431E-4 0.0 0.0 0.0 7.798952366728578E-5 20-21 3.119580946691431E-4 0.0 0.0 1.364816664177501E-4 7.798952366728578E-5 22-23 3.119580946691431E-4 0.0 0.0 2.3396857100185732E-4 9.748690458410723E-5 24-25 3.50952856502786E-4 0.0 0.0 2.534659519186788E-4 1.1698428550092866E-4 26-27 3.7045023741960744E-4 0.0 0.0 4.874345229205361E-4 1.1698428550092866E-4 28-29 4.874345229205361E-4 0.0 0.0 0.0014623035687616082 1.1698428550092866E-4 30-31 5.459266656710004E-4 0.0 0.0 0.0036655076123624313 1.1698428550092866E-4 32-33 5.654240465878218E-4 0.0 0.0 0.006726596416303398 1.5597904733457156E-4 34-35 6.044188084214648E-4 0.0 0.0 0.009748690458410722 1.5597904733457156E-4 36-37 6.239161893382862E-4 0.0 0.0 0.012595308072266654 1.5597904733457156E-4 38-39 6.62910951171929E-4 0.0 0.0 0.015636899495290797 1.5597904733457156E-4 40-41 6.62910951171929E-4 0.0 0.0 0.020316270915327943 1.5597904733457156E-4 42-43 7.214030939223934E-4 0.0 0.0 0.0264579459041267 1.75476428251393E-4 44-45 7.409004748392149E-4 0.0 0.0 0.03203419684633763 1.9497380916821443E-4 46-47 8.578847603401434E-4 0.0 0.0 0.03786391374046724 2.1447119008503586E-4 48-49 8.968795221737864E-4 0.0 0.0 0.043888604443765064 2.3396857100185732E-4 50-51 8.968795221737864E-4 0.0 0.0 0.05213599657158054 2.534659519186788E-4 52-53 8.968795221737864E-4 0.0 0.0 0.07249126224874212 2.729633328355002E-4 54-55 8.968795221737864E-4 0.0 0.0 0.10376506123932372 2.729633328355002E-4 56-57 9.358742840074293E-4 0.0 0.0 0.12858522714643741 2.729633328355002E-4 58-59 9.358742840074293E-4 0.0 0.0 0.16307609398829453 2.729633328355002E-4 60-61 9.358742840074293E-4 0.0 0.0 0.20450802843654015 2.729633328355002E-4 62-63 9.358742840074293E-4 0.0 0.0 0.2307710005314986 2.729633328355002E-4 64-65 9.358742840074293E-4 0.0 0.0 0.2524130933491704 2.9246071375232165E-4 66-67 9.748690458410722E-4 0.0 0.0 0.313498387761572 3.119580946691431E-4 68-69 9.748690458410722E-4 0.0 0.0 0.38265559787353765 3.119580946691431E-4 70-71 0.0010138638076747151 0.0 0.0 0.41197965877243714 3.119580946691431E-4 72-73 0.0010723559504251792 0.0 0.0 0.47082275437940424 3.50952856502786E-4 74-75 0.0010918533313420008 0.0 0.0 0.5553244032729083 3.50952856502786E-4 76-77 0.0010918533313420008 0.0 0.0 0.6393971097862424 3.50952856502786E-4 78-79 0.0010918533313420008 0.0 0.0 0.7148909686961751 3.50952856502786E-4 80-81 0.0012283349977597509 0.0 0.0 0.821366165882937 3.50952856502786E-4 82-83 0.0013063245214270367 0.0 0.0 0.9466368382735146 3.50952856502786E-4 84-85 0.001325821902343858 0.0 0.0 1.0823191120736753 3.50952856502786E-4 86-87 0.0013453192832606794 0.0 0.0 1.2844679574192799 3.50952856502786E-4 88-89 0.0013843140450943226 0.0 0.0 1.5294330512582244 3.50952856502786E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5435 0.0 51.4873 1 GTATCAA 9350 0.0 39.379887 1 ATCAACG 11865 0.0 30.865217 3 TATCAAC 12170 0.0 30.247805 2 TCAACGC 12105 0.0 30.212263 4 CAACGCA 12340 0.0 29.63691 5 AACGCAG 12595 0.0 29.074585 6 ACGCAGA 14270 0.0 25.428852 7 CGCAGAG 14525 0.0 24.753525 8 GCAGAGT 15400 0.0 22.88445 9 GAGTACT 9450 0.0 22.84333 12-13 GTACATG 8530 0.0 21.332125 1 GTACTTT 10165 0.0 20.91008 14-15 AGAGTAC 14075 0.0 20.39883 10-11 CAGAGTA 15000 0.0 20.011662 10-11 TACATGG 8815 0.0 19.990992 2 AGTACTT 9980 0.0 19.98831 12-13 TATACCG 170 7.963837E-5 19.557192 5 ACATGGG 8585 0.0 19.475342 3 GTGGTAT 2770 0.0 18.180695 1 >>END_MODULE