FastQCFastQC Report
Thu 26 May 2016
SRR936810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420643
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79960.330325454848154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59930.24757884578601635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37680.1556611197933772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.051.3104481
GTATCAA78450.041.91681
ATCAACG97300.033.455053
TCAACGC98750.032.916374
TATCAAC100650.032.6255042
CAACGCA101900.031.9494385
AACGCAG102750.031.7287676
ACGCAGA119350.026.9180857
CGCAGAG121300.026.132748
GTACATG83900.023.9015181
GCAGAGT130250.023.6437959
TACATGG86850.022.2151452
GTGTAGC13100.021.7648121
GTGGTAT22950.021.7411061
GAGTACT74850.021.45941412-13
ACATGGG84850.021.1141433
TGGTATC23650.020.4956632
CAGAGTA125000.020.20606410-11
AGAGTAC117400.019.85451910-11
GTACTTT81150.019.64703214-15
Produced by FastQC (version 0.11.5)