FastQCFastQC Report
Thu 26 May 2016
SRR936810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420643
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93860.38774821400760046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76110.31442058990111305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48630.20089703438301312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48300.048.398481
GTATCAA84900.040.014011
ATCAACG109150.030.7649973
TATCAAC110800.030.4802112
TCAACGC110600.030.3585224
CAACGCA113200.029.6612425
AACGCAG115450.029.1237136
ACGCAGA133050.025.0570327
CGCAGAG134850.024.6169138
GTACATG81750.024.5266631
TACATGG85200.022.9134432
ACATGGG83400.021.9827733
GCAGAGT149000.021.7691679
GAGTACT91150.021.20708712-13
GTGGTAT27150.020.125271
TGGTATC25700.019.9608382
AGTACTT95450.019.77900512-13
AGAGTAC135100.019.61490410-11
CAGAGTA141700.019.03644610-11
GTACTTT100700.019.00899914-15
Produced by FastQC (version 0.11.5)