##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936810_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2420643 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2084594878303 33.0 31.0 34.0 31.0 34.0 2 32.27579903356257 33.0 31.0 34.0 30.0 34.0 3 32.42202381763854 34.0 31.0 34.0 31.0 34.0 4 36.09164837607198 37.0 35.0 37.0 35.0 37.0 5 36.006010799609854 37.0 35.0 37.0 35.0 37.0 6 36.03388975573846 37.0 35.0 37.0 35.0 37.0 7 35.99132668468667 37.0 35.0 37.0 35.0 37.0 8 35.9736983107381 37.0 35.0 37.0 35.0 37.0 9 37.679978418957276 39.0 37.0 39.0 35.0 39.0 10-11 37.57309462816285 39.0 37.0 39.0 35.0 39.0 12-13 37.49428974037064 39.0 37.0 39.0 35.0 39.0 14-15 38.738797914438436 40.0 38.0 41.0 35.0 41.0 16-17 38.65339746505371 40.0 38.0 41.0 34.0 41.0 18-19 38.669237264644146 40.0 38.0 41.0 34.5 41.0 20-21 38.761875666919906 40.0 38.0 41.0 34.5 41.0 22-23 38.70117567935462 40.0 38.0 41.0 34.0 41.0 24-25 38.61709120262674 40.0 38.0 41.0 34.0 41.0 26-27 38.456721623138975 40.0 38.0 41.0 34.0 41.0 28-29 38.30344334129403 40.0 38.0 41.0 34.0 41.0 30-31 38.27820831076701 40.0 38.0 41.0 34.0 41.0 32-33 38.21445417601852 40.0 38.0 41.0 34.0 41.0 34-35 38.108216907656356 40.0 38.0 41.0 33.5 41.0 36-37 37.95356151237502 40.0 37.0 41.0 33.0 41.0 38-39 37.82046113367399 40.0 37.0 41.0 33.0 41.0 40-41 37.600786443932456 40.0 37.0 41.0 33.0 41.0 42-43 37.39544616864197 40.0 37.0 41.0 32.5 41.0 44-45 37.17705977296115 40.0 36.5 41.0 31.5 41.0 46-47 36.85963997995574 39.5 36.0 41.0 31.0 41.0 48-49 36.52675280906767 39.0 36.0 41.0 30.5 41.0 50-51 36.29573588505203 39.0 35.5 41.0 30.0 41.0 52-53 36.06851505984154 39.0 35.0 40.0 29.5 41.0 54-55 35.802992428044945 39.0 35.0 40.0 29.0 41.0 56-57 35.55173976501285 38.0 35.0 40.0 28.0 41.0 58-59 35.2024360882625 38.0 34.0 40.0 28.0 41.0 60-61 34.869502855233094 38.0 34.0 40.0 27.0 41.0 62-63 34.52575472715307 37.0 34.0 40.0 26.0 41.0 64-65 34.111426385468654 36.5 33.5 39.5 26.0 41.0 66-67 33.67798349446821 36.0 33.0 39.0 26.0 40.0 68-69 33.24176448158609 35.5 32.5 39.0 25.0 40.0 70-71 32.84516696596731 35.0 32.0 38.0 24.0 40.0 72-73 32.43966334564824 35.0 32.0 37.5 23.5 39.5 74-75 31.95225400854236 35.0 31.0 37.0 22.0 39.0 76-77 30.506315264167412 33.5 29.5 35.5 20.5 37.5 78-79 31.21603020354509 34.0 31.0 36.0 21.0 38.0 80-81 30.926425953765175 34.0 31.0 35.5 19.0 37.0 82-83 30.724070009497474 34.0 31.0 35.0 19.5 37.0 84-85 30.39242424430203 34.0 30.5 35.0 18.0 36.5 86-87 30.018896632010584 34.0 30.0 35.0 16.0 36.0 88-89 29.6424222820135 34.0 30.0 35.0 9.0 36.0 90-91 29.196557484932722 34.0 29.0 35.0 4.5 35.0 92-93 28.748454233028166 34.0 29.0 35.0 2.0 35.0 94-95 28.2453660039915 33.5 29.0 35.0 2.0 35.0 96-97 27.601948944970406 33.0 27.0 35.0 2.0 35.0 98-99 26.78927603120328 33.0 26.0 34.5 2.0 35.0 100-101 23.4305263105712 29.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2581677627439518 1101 2 0.1829540151250697 1101 3 0.15112646650152328 1101 4 0.08487867709801122 1101 5 0.17028979587450976 1101 6 0.1839016747054103 1101 7 0.15572190948955011 1101 8 0.1569647652374826 1101 9 0.22687663005532954 1101 10-11 0.15858952164795426 1101 12-13 0.1804745991631549 1101 14-15 0.36241156993892076 1101 16-17 0.293495624528596 1101 18-19 0.2927353192029898 1101 20-21 0.2687840660059422 1101 22-23 0.277950481719202 1101 24-25 0.296148620179558 1101 26-27 0.19501789961445581 1101 28-29 0.24490003541397698 1101 30-31 0.2299396606366173 1101 32-33 0.3903296682591417 1101 34-35 0.35261914274306605 1101 36-37 0.3305104831252166 1101 38-39 0.33903324495726395 1101 40-41 0.45321064143796264 1101 42-43 0.542205036644404 1101 44-45 0.45329199298208067 1101 46-47 0.36219810597413726 1101 48-49 0.48377556644374664 1101 50-51 0.36039449858562733 1101 52-53 0.35205908232619265 1101 54-55 0.4629583793003462 1101 56-57 0.4548867268125534 1101 58-59 0.4414097100040095 1101 60-61 0.2660650257534627 1101 62-63 0.27399223749358015 1101 64-65 0.40723156034633945 1101 66-67 0.41520856500034853 1101 68-69 0.38658940803901487 1101 70-71 0.45374676903836786 1101 72-73 0.5697293182691325 1101 74-75 0.40165486250994675 1101 76-77 0.44512541443042863 1101 78-79 0.6146558801538369 1101 80-81 0.9138474006493418 1101 82-83 0.8677195813402001 1101 84-85 0.6247370540353323 1101 86-87 0.5615278529154324 1101 88-89 0.3932330369945518 1101 90-91 0.3795087662660528 1101 92-93 0.5410994793490147 1101 94-95 0.7724456665792694 1101 96-97 0.7414096296420105 1101 98-99 0.9188177996439926 1101 100-101 1.1723216387006516 1102 1 0.24520784311075516 1102 2 0.25617680117422026 1102 3 0.17394033492664107 1102 4 0.15004044104628633 1102 5 0.2082391544856037 1102 6 0.2596897587814482 1102 7 0.21692831307514382 1102 8 0.23933648745684621 1102 9 0.3071777479781588 1102 10-11 0.2908734714702206 1102 12-13 0.24595873187140427 1102 14-15 0.25938718943302064 1102 16-17 0.3115902615900623 1102 18-19 0.30484668646897717 1102 20-21 0.26372095745923474 1102 22-23 0.33858407864877194 1102 24-25 0.2638940995469241 1102 26-27 0.35112693572799714 1102 28-29 0.35387574506314934 1102 30-31 0.4120390501580218 1102 32-33 0.39283926333409624 1102 34-35 0.4359787713041925 1102 36-37 0.44440750028044107 1102 38-39 0.4173529477031295 1102 40-41 0.5130379944852024 1102 42-43 0.42286278225256524 1102 44-45 0.5248108482703131 1102 46-47 0.46688745737282744 1102 48-49 0.5805565797866947 1102 50-51 0.4901050364249997 1102 52-53 0.4502242979255868 1102 54-55 0.4265717943742757 1102 56-57 0.44303197950431894 1102 58-59 0.45031954205799707 1102 60-61 0.34500963190461675 1102 62-63 0.3917500165878991 1102 64-65 0.4736293502592801 1102 66-67 0.3771434859214722 1102 68-69 0.3175400288210497 1102 70-71 0.40747043087959156 1102 72-73 0.4320587320036182 1102 74-75 0.48349093484006644 1102 76-77 0.497131751043689 1102 78-79 0.47610351293937114 1102 80-81 0.6405781203443581 1102 82-83 0.6215991470503646 1102 84-85 0.5296993177002634 1102 86-87 0.6522249447705661 1102 88-89 0.608511938133077 1102 90-91 0.5698057628144007 1102 92-93 0.7213043889363604 1102 94-95 0.9857816616336201 1102 96-97 0.8988812307959968 1102 98-99 0.7328571974081477 1102 100-101 1.0908967378712227 1103 1 0.131703483620484 1103 2 0.12147848306040743 1103 3 0.15988059185281145 1103 4 0.1392931367074297 1103 5 0.14129105563609556 1103 6 0.1282347466574265 1103 7 0.13713635620572262 1103 8 0.12608634557994236 1103 9 0.19313838358667823 1103 10-11 0.09499415712259918 1103 12-13 0.13269130680820496 1103 14-15 0.25763210766033495 1103 16-17 0.1834390466078588 1103 18-19 0.12617368693329922 1103 20-21 0.17257613983554876 1103 22-23 0.14338396772683382 1103 24-25 0.1649105830634099 1103 26-27 0.21129705903192786 1103 28-29 0.13536687130779512 1103 30-31 0.0865731135125074 1103 32-33 0.09468733896738968 1103 34-35 0.16547272218899423 1103 36-37 0.23897928999307538 1103 38-39 0.1570357238471729 1103 40-41 0.24018994464771737 1103 42-43 0.18039683270914963 1103 44-45 0.29492473672927133 1103 46-47 0.2712972494586765 1103 48-49 0.2903584035497815 1103 50-51 0.2542155327361897 1103 52-53 0.264690324856403 1103 54-55 0.21627926999723712 1103 56-57 0.17173561155302508 1103 58-59 0.30253366009958427 1103 60-61 0.4002065183514958 1103 62-63 0.3119403251056738 1103 64-65 0.2528215547683814 1103 66-67 0.2944914841673594 1103 68-69 0.21700489494084252 1103 70-71 0.24412162006598948 1103 72-73 0.3645464145089079 1103 74-75 0.2404913991644797 1103 76-77 0.34949026107825887 1103 78-79 0.40510418363018275 1103 80-81 0.5958373681388203 1103 82-83 0.48773280799036556 1103 84-85 0.46382754862427333 1103 86-87 0.4503791907593211 1103 88-89 0.39288361692377194 1103 90-91 0.31853841513860104 1103 92-93 0.4103490414669295 1103 94-95 0.6205675564899416 1103 96-97 0.7227549216591278 1103 98-99 1.0048664967942038 1103 100-101 0.926644751594587 1104 1 0.10240836178868307 1104 2 0.046073203535854645 1104 3 0.06887384483118097 1104 4 0.12658874982518142 1104 5 0.04179016701112204 1104 6 0.07400623508228676 1104 7 -0.025165021361758022 1104 8 0.04104826254926763 1104 9 0.05871308980898249 1104 10-11 0.017455508108739082 1104 12-13 0.10077236647936161 1104 14-15 0.1251851395516539 1104 16-17 0.13944133481192722 1104 18-19 0.1484242536487983 1104 20-21 0.18288665850211316 1104 22-23 0.11484690765059469 1104 24-25 0.10270955504545753 1104 26-27 0.20121397981562694 1104 28-29 0.2104553676421972 1104 30-31 0.17386759675626706 1104 32-33 0.12414733870792816 1104 34-35 -0.032891931669929875 1104 36-37 0.1160669937417822 1104 38-39 0.2302065729307401 1104 40-41 0.2607431895412162 1104 42-43 0.24983030970387432 1104 44-45 0.2094252884844039 1104 46-47 0.23013719527494203 1104 48-49 0.2760437369902533 1104 50-51 0.1406418542807586 1104 52-53 0.25510731950038235 1104 54-55 0.19914807865391282 1104 56-57 0.19162285849787253 1104 58-59 0.22117101532401762 1104 60-61 0.22556231357983592 1104 62-63 0.20025709323083873 1104 64-65 0.23503378109302275 1104 66-67 0.14706740856121314 1104 68-69 0.13083128854625414 1104 70-71 0.11778936262378892 1104 72-73 0.15877250731675474 1104 74-75 0.15733853181628987 1104 76-77 0.2466194186483932 1104 78-79 0.35975522623187217 1104 80-81 0.43365818435142245 1104 82-83 0.31695277227797547 1104 84-85 0.14957778704860658 1104 86-87 0.12450595508894935 1104 88-89 0.2194180381165225 1104 90-91 0.30572062238510966 1104 92-93 0.34982629480183647 1104 94-95 0.34686729866943367 1104 96-97 0.5876341276673749 1104 98-99 0.8434476763575454 1104 100-101 0.7938558919847836 1105 1 0.220770721628746 1105 2 0.25102865962333 1105 3 0.1546742145133848 1105 4 0.09311074148565979 1105 5 0.2091315663131823 1105 6 0.20208407062089861 1105 7 0.19405662325208795 1105 8 0.1364576725892519 1105 9 0.15512039136967815 1105 10-11 0.20554794599818393 1105 12-13 0.1612018740160508 1105 14-15 0.3372869341189286 1105 16-17 0.21052328617146543 1105 18-19 0.3387908439324576 1105 20-21 0.3143776490635588 1105 22-23 0.20894730748145918 1105 24-25 0.3206590324527525 1105 26-27 0.38722033318673965 1105 28-29 0.28109905583384176 1105 30-31 0.18927230630337277 1105 32-33 0.18339546212284574 1105 34-35 0.23286675966662074 1105 36-37 0.33418840516109327 1105 38-39 0.38157616941076355 1105 40-41 0.5275527492649275 1105 42-43 0.39057178355881916 1105 44-45 0.4396336127875884 1105 46-47 0.4582038093500316 1105 48-49 0.506170669562259 1105 50-51 0.49401802181865406 1105 52-53 0.4397938264851433 1105 54-55 0.4861312291207369 1105 56-57 0.3851988836724729 1105 58-59 0.4865015572580944 1105 60-61 0.5142643225589296 1105 62-63 0.4460329105434866 1105 64-65 0.5349851868582576 1105 66-67 0.41521126290757593 1105 68-69 0.5081934547799563 1105 70-71 0.5992790046831757 1105 72-73 0.6161148120416229 1105 74-75 0.44825521461681817 1105 76-77 0.44521452031099784 1105 78-79 0.46682195492963174 1105 80-81 0.6390022979954679 1105 82-83 0.7875243637704692 1105 84-85 0.5117445023576686 1105 86-87 0.529958141540547 1105 88-89 0.36433770110659935 1105 90-91 0.5916374930127155 1105 92-93 0.6854674930807647 1105 94-95 0.6778354557198334 1105 96-97 0.8276166838300796 1105 98-99 1.0759421932518727 1105 100-101 1.1582938700408008 1106 1 0.11079012467895666 1106 2 0.044555406137327225 1106 3 0.06568839906906732 1106 4 0.059448099115911646 1106 5 -0.0147219454473273 1106 6 0.11871375122093752 1106 7 0.06169067512624338 1106 8 0.1102289635253797 1106 9 0.08292465365464352 1106 10-11 0.11088437019590458 1106 12-13 0.07973487357008224 1106 14-15 0.15170282567471105 1106 16-17 0.09118388393016374 1106 18-19 0.17088953446604194 1106 20-21 0.04397703940546194 1106 22-23 0.1595860754782379 1106 24-25 0.07920520862563762 1106 26-27 0.04223372799676639 1106 28-29 0.015829132051749184 1106 30-31 -0.10128597575503306 1106 32-33 0.05566767318715904 1106 34-35 -0.04896307675385003 1106 36-37 0.04194393098588023 1106 38-39 0.11154834737388342 1106 40-41 0.10735564279933385 1106 42-43 0.029702456662661803 1106 44-45 -0.007990657735412299 1106 46-47 0.0043318205214148975 1106 48-49 -0.017399566180010595 1106 50-51 -0.17344131745586822 1106 52-53 -0.18383063774906105 1106 54-55 -0.07901932811825674 1106 56-57 -0.019669227739136375 1106 58-59 0.021270322498210703 1106 60-61 -0.031603455711994854 1106 62-63 0.1484746827667962 1106 64-65 0.02819172450474383 1106 66-67 0.08580111350516262 1106 68-69 0.10199783627181347 1106 70-71 0.07246463854985308 1106 72-73 0.1199717403567675 1106 74-75 0.15821686618572173 1106 76-77 0.04383347654823311 1106 78-79 0.022688477270811802 1106 80-81 0.22167866299419714 1106 82-83 0.27511913058795656 1106 84-85 0.04757947744662516 1106 86-87 -0.02419318522150604 1106 88-89 -0.06153051354669614 1106 90-91 -0.05151641266270346 1106 92-93 0.023960781839232226 1106 94-95 0.01788854536022555 1106 96-97 0.06817006989117758 1106 98-99 0.15767847354984355 1106 100-101 0.08641690432448712 1107 1 0.13920297502973966 1107 2 -0.03090760894658473 1107 3 0.06647233805183106 1107 4 0.019540110497572982 1107 5 0.09984231573327662 1107 6 0.02949248130083504 1107 7 0.06953078317551586 1107 8 0.05695127800916566 1107 9 0.04862705382586796 1107 10-11 0.09802541870261905 1107 12-13 0.2114456902222699 1107 14-15 0.19442723090185865 1107 16-17 0.20455167754053605 1107 18-19 0.16454566176797414 1107 20-21 0.23194140339949598 1107 22-23 0.19660717198371458 1107 24-25 0.1741821276736104 1107 26-27 0.24071510208415248 1107 28-29 0.1797187945252574 1107 30-31 0.23805285807917187 1107 32-33 0.2974700253991216 1107 34-35 0.2741019307488699 1107 36-37 0.28358683798101936 1107 38-39 0.19403431377079983 1107 40-41 0.3315965767317408 1107 42-43 0.25956105137931473 1107 44-45 0.34795992573891255 1107 46-47 0.32402280204497913 1107 48-49 0.2174144889158498 1107 50-51 0.19276888341098442 1107 52-53 0.11289843699644564 1107 54-55 0.16010145360860406 1107 56-57 0.12106326143231882 1107 58-59 0.16933127653723545 1107 60-61 0.13389906336082902 1107 62-63 0.1687112973139122 1107 64-65 -0.044417257108953834 1107 66-67 -0.08533585478409833 1107 68-69 0.10183040083859396 1107 70-71 -0.05309029993355807 1107 72-73 0.03228943997886091 1107 74-75 0.020333293154873644 1107 76-77 0.08939961569902266 1107 78-79 0.3079179248631654 1107 80-81 0.3270118002530076 1107 82-83 0.2664767689026384 1107 84-85 0.26807587278170075 1107 86-87 0.20023466590765793 1107 88-89 -0.08921831909391642 1107 90-91 -0.06406015460852288 1107 92-93 0.08096136952413602 1107 94-95 0.14813005997486783 1107 96-97 0.27450439024067563 1107 98-99 0.6071288633736494 1107 100-101 0.6684719377029467 1108 1 0.04105625234344501 1108 2 -0.013010659836389493 1108 3 -0.054331655092077824 1108 4 0.006862025575294695 1108 5 0.006458118927277212 1108 6 -0.0197503535080088 1108 7 0.0281213885586169 1108 8 0.05525819675191457 1108 9 0.05270176544540561 1108 10-11 0.024183671095919124 1108 12-13 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-0.9991175112743065 2316 64-65 -0.9691927315409643 2316 66-67 -0.9810182082074661 2316 68-69 -1.2984119521953232 2316 70-71 -0.9291845043307738 2316 72-73 -0.9179747959652396 2316 74-75 -1.0684452886975002 2316 76-77 -0.9631884643885051 2316 78-79 -0.8450653667065531 2316 80-81 -0.7671491766214587 2316 82-83 -0.9243891614869355 2316 84-85 -1.1671154586021935 2316 86-87 -1.111152314867887 2316 88-89 -1.0318275408836115 2316 90-91 -1.156113556761138 2316 92-93 -1.1827254996148042 2316 94-95 -1.0702891003777886 2316 96-97 -1.184454435497397 2316 98-99 -1.2338257999789413 2316 100-101 -1.5101395629873053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 18.0 5 45.0 6 100.0 7 247.0 8 570.0 9 1117.0 10 1871.0 11 2949.0 12 4498.0 13 6567.0 14 8348.0 15 11228.0 16 14772.0 17 20110.0 18 18864.0 19 12490.0 20 11025.0 21 11709.0 22 12128.0 23 13370.0 24 15893.0 25 18660.0 26 23006.0 27 28730.0 28 36494.0 29 45303.0 30 57313.0 31 72583.0 32 92726.0 33 122935.0 34 167499.0 35 240765.0 36 372721.0 37 551821.0 38 394176.0 39 27983.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.49522110992563 19.455227192525015 14.966954791245355 23.082596906304005 2 15.751216904373749 20.538602578159924 39.62505876918933 24.085121748277 3 19.396163677686786 24.338381691222615 29.98742765115223 26.278026979938364 4 13.445642335528204 17.133794615728135 36.64427179059449 32.77629125814918 5 14.170462315149338 36.93753653634424 34.067716434589876 14.824284713916553 6 32.20788856514571 36.094913624189935 17.304658307730634 14.392539502933724 7 28.39819832994787 32.51028755582711 20.784890626168337 18.306623488056687 8 26.26133893405138 35.10180357111874 19.706218432961172 18.93063906186871 9 26.096330603067035 16.762570936730448 20.373016591046262 36.768081869156255 10-11 24.909228663623672 26.691069273742556 27.347134624973613 21.05256743766016 12-13 25.751702650293023 23.893308293102894 28.06574134587658 22.2892477107275 14-15 22.695953100064735 25.27130188135962 26.199402390191366 25.833342628384276 16-17 22.264290934268292 28.55007946235773 26.779186356682917 22.406443246691065 18-19 22.156437337418414 26.9075946838597 29.270128615164825 21.665839363557062 20-21 22.626456708657443 26.19578280029298 29.53258495479034 21.645175536259234 22-23 22.54793457771344 26.049896659689182 29.393099271557187 22.00906949104019 24-25 22.35493322470591 26.298034377935853 29.445392822822996 21.901639574535242 26-27 22.27654982926052 26.46355578112874 29.289624191360424 21.970270198250322 28-29 22.079564475790452 26.389401594214608 29.45760470360858 22.073429226386367 30-31 22.235485159365656 26.321714132576705 29.37046551669632 22.07233519136132 32-33 22.10867028143558 26.43085979860573 29.323067389620448 22.13740253033824 34-35 22.436199870441488 26.410353443546203 29.155973407454905 21.997473278557404 36-37 22.455632144585103 26.249949902428522 29.12462085951319 22.169797093473186 38-39 22.27956465928429 26.410492433428672 29.255563740446505 22.054379166840533 40-41 22.505689697521074 26.348613763066886 29.1591807661885 21.98651577322354 42-43 22.34114412913717 26.415433035212615 28.97137611464758 22.272046721002635 44-45 22.465948837584957 26.568158819426124 28.754324108774508 22.211568234214408 46-47 22.558512497345692 26.74952924777968 28.347194944711916 22.344763310162712 48-49 22.69454338068847 26.90616685336199 28.094586055873094 22.304703710076446 50-51 22.64363936517589 27.00731148288672 28.028762575519217 22.320286576418173 52-53 22.786355159800674 26.98849200480216 27.86371684779421 22.361435987602963 54-55 22.682259693003257 26.98501453351467 27.831474023924528 22.50125174955755 56-57 22.521761420756743 27.32737881179885 27.81780990078804 22.333049866656367 58-59 22.721054374281305 26.97748366586171 27.913689898932937 22.38777206092405 60-61 22.697842142704292 26.896831501725575 28.20317915660391 22.202147198966223 62-63 22.547927894320576 26.982620102976835 28.17779914952297 22.29165285317962 64-65 22.601935803187285 27.01931919682511 27.99149677710418 22.38724822288343 66-67 22.471072505774345 27.229396919220317 27.783242399367765 22.516288175637573 68-69 22.406697457676135 27.03164891639332 28.068511373119282 22.493142252811264 70-71 22.511716277693957 27.033307113548744 27.91264763088272 22.54232897787458 72-73 22.597885132466363 26.969704857237765 27.72844684405971 22.703963166236157 74-75 22.61221860913415 27.15473239025813 27.64985306882279 22.583195931784932 76-77 22.594275704795585 27.122050269359153 27.71453713190336 22.5691368939419 78-79 22.577505187670234 27.087684796227073 27.624930151080246 22.70987986502245 80-81 22.60249822412631 27.294853281539854 27.51239909949416 22.59024939483967 82-83 22.583835597083464 27.261281672155107 27.601701437819067 22.553181292942362 84-85 22.52943403654718 27.250075342127623 27.477351402499643 22.743139218825554 86-87 22.471591225967646 27.09408202696556 27.71430979289387 22.72001695417292 88-89 22.625898998228134 27.194497587592554 27.432825844383917 22.746777569795395 90-91 22.462197224061168 27.19963563091725 27.61376464112021 22.724402503901374 92-93 22.467217320392393 27.008065642198464 27.66340939867919 22.861307638729954 94-95 22.430473707062333 27.012749981306527 27.68115008028898 22.875626231342157 96-97 22.49338810677185 26.90394020088919 27.642753687251048 22.959918005087914 98-99 22.25461727636155 26.878400189702134 27.509060631561034 23.357921902375285 100-101 22.094291428281064 27.1573268534588 27.49806558063771 23.250316137622423 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 568.0 1 432.0 2 329.5 3 369.0 4 327.0 5 320.5 6 580.5 7 1255.0 8 2121.5 9 2688.5 10 2518.5 11 2176.5 12 2276.5 13 2450.0 14 2662.5 15 2907.5 16 3142.5 17 3322.5 18 3474.0 19 3701.0 20 4123.5 21 4815.0 22 5711.5 23 6888.0 24 8422.0 25 10517.0 26 13005.5 27 16188.5 28 19473.0 29 22743.0 30 26946.5 31 31786.5 32 36360.5 33 40510.0 34 45763.5 35 50516.5 36 55127.0 37 60354.5 38 64656.0 39 67278.5 40 69322.5 41 73905.0 42 78394.0 43 82633.5 44 87261.5 45 90064.5 46 91155.0 47 92269.5 48 95472.5 49 95603.5 50 93361.0 51 91030.0 52 86910.0 53 84262.5 54 81336.5 55 75931.5 56 69650.5 57 62946.5 58 56764.0 59 50538.0 60 42992.5 61 34556.5 62 27000.5 63 21106.0 64 15902.5 65 11860.0 66 8934.5 67 6846.5 68 4830.0 69 2993.5 70 2010.5 71 1395.5 72 942.5 73 658.0 74 462.5 75 332.5 76 213.0 77 116.0 78 66.0 79 31.5 80 21.0 81 11.5 82 5.0 83 3.0 84 2.5 85 2.0 86 2.5 87 3.5 88 2.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02581958595298852 2 0.006485879991390718 3 0.010410457056244974 4 0.0 5 0.004461624452676417 6 0.0 7 0.0 8 4.1311337524781634E-5 9 0.0 10-11 0.0 12-13 2.0655668762390822E-4 14-15 0.0 16-17 0.0 18-19 0.005267195534409659 20-21 0.0013219628007930123 22-23 0.0 24-25 0.005659653240895085 26-27 0.005886865597281384 28-29 0.008406857186293063 30-31 0.032367432950666415 32-33 7.436040754460696E-4 34-35 0.004750803815349889 36-37 0.01511994953407008 38-39 0.008014399479807638 40-41 0.010720292087680836 42-43 0.006072766616142901 44-45 0.0023754019076749444 46-47 0.0020242555387143006 48-49 0.001342618469555403 50-51 0.00276785961416037 52-53 0.002685236939110806 54-55 0.001280651463268231 56-57 7.229484066836787E-4 58-59 9.295050943075869E-4 60-61 4.1311337524781634E-5 62-63 6.196700628717245E-5 64-65 0.006155389291192464 66-67 9.088494255451961E-4 68-69 9.501607630699777E-4 70-71 0.0032635956644577494 72-73 0.010534391068819317 74-75 0.00503998317802336 76-77 0.003428841014556876 78-79 0.009935376674709983 80-81 1.4458968133673574E-4 82-83 0.0042137564275277276 84-85 7.642597442084604E-4 86-87 0.0 88-89 0.0013219628007930123 90-91 8.468824192580236E-4 92-93 2.0655668762390822E-4 94-95 4.957360502973796E-4 96-97 2.0655668762390822E-4 98-99 2.478680251486898E-4 100-101 8.262267504956329E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2420643.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.22307725581675 #Duplication Level Percentage of deduplicated Percentage of total 1 76.49915864513498 43.01018106510405 2 13.399971273728328 15.067752402971058 3 4.294624039517376 7.243709374754196 4 1.8682047881702006 4.201448885399198 5 1.0271583617184221 2.88750019624265 6 0.6340878210120662 2.139022112864034 7 0.41530225444422525 1.6344699515292778 8 0.2995015071635698 1.347111710039275 9 0.2104542544210305 1.0649147224616018 >10 1.223223689375118 12.51124548214412 >50 0.08627010299572421 3.3656296239808703 >100 0.04057292013708137 4.089308523002285 >500 0.0011027566365037823 0.39899305783360856 >1k 2.205513272998899E-4 0.3361660146431771 >5k 1.470342181999266E-4 0.702546877030626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9386 0.38774821400760046 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7611 0.31442058990111305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4863 0.20089703438301312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.262267504956328E-5 0.0 0.0 0.0 4.131133752478164E-5 7 1.239340125743449E-4 0.0 0.0 0.0 4.131133752478164E-5 8 1.239340125743449E-4 0.0 0.0 0.0 4.131133752478164E-5 9 1.239340125743449E-4 0.0 0.0 4.131133752478164E-5 8.262267504956328E-5 10-11 1.239340125743449E-4 0.0 0.0 6.196700628717245E-5 8.262267504956328E-5 12-13 1.6524535009912656E-4 0.0 0.0 1.032783438119541E-4 8.262267504956328E-5 14-15 1.6524535009912656E-4 0.0 0.0 1.6524535009912656E-4 8.262267504956328E-5 16-17 1.6524535009912656E-4 0.0 0.0 1.6524535009912656E-4 8.262267504956328E-5 18-19 1.6524535009912656E-4 0.0 0.0 1.6524535009912656E-4 8.262267504956328E-5 20-21 2.065566876239082E-4 0.0 0.0 2.2721235638629902E-4 8.262267504956328E-5 22-23 2.065566876239082E-4 0.0 0.0 4.131133752478164E-4 8.262267504956328E-5 24-25 2.065566876239082E-4 0.0 0.0 6.196700628717245E-4 8.262267504956328E-5 26-27 2.065566876239082E-4 0.0 0.0 0.0010740947756443225 8.262267504956328E-5 28-29 3.304907001982531E-4 0.0 0.0 0.0019829442011895185 8.262267504956328E-5 30-31 4.131133752478164E-4 0.0 0.0 0.00452359145896359 8.262267504956328E-5 32-33 4.131133752478164E-4 0.0 0.0 0.00733276241064874 8.262267504956328E-5 34-35 4.5442471277259804E-4 0.0 0.0 0.011050782787879087 8.262267504956328E-5 36-37 5.370473878221613E-4 0.0 0.0 0.013632741383177941 8.262267504956328E-5 38-39 6.196700628717245E-4 0.0 0.0 0.01640060099733831 8.262267504956328E-5 40-41 6.196700628717245E-4 0.0 0.0 0.021068782137638638 8.262267504956328E-5 42-43 6.81637069158897E-4 0.0 0.0 0.026811058053583282 8.262267504956328E-5 44-45 8.262267504956328E-4 0.0 0.0 0.03253267830076554 8.262267504956328E-5 46-47 8.881937567828053E-4 0.0 0.0 0.03932839332359212 8.262267504956328E-5 48-49 9.501607630699777E-4 0.0 0.0 0.044802145545625685 8.262267504956328E-5 50-51 9.501607630699777E-4 0.0 0.0 0.05062704413661989 8.262267504956328E-5 52-53 9.708164318323685E-4 0.0 0.0 0.07138599124282267 1.239340125743449E-4 54-55 9.914721005947593E-4 0.0 0.0 0.1053232550194308 1.6524535009912656E-4 56-57 9.914721005947593E-4 0.0 0.0 0.12847825970207088 1.8590101886151736E-4 58-59 9.914721005947593E-4 0.0 0.0 0.1647289583800668 2.065566876239082E-4 60-61 9.914721005947593E-4 0.0 0.0 0.20711439068049275 2.065566876239082E-4 62-63 9.914721005947593E-4 0.0 0.0 0.23566052491011685 2.065566876239082E-4 64-65 9.914721005947593E-4 0.0 0.0 0.25695651940414177 2.065566876239082E-4 66-67 0.0010740947756443227 0.0 0.0 0.3221458100182472 2.065566876239082E-4 68-69 0.0011154061131691043 0.0 0.0 0.394543929030427 2.065566876239082E-4 70-71 0.0011773731194562767 0.0 0.0 0.42294547357871437 2.065566876239082E-4 72-73 0.0012599957945058399 0.0 0.0 0.47848856688078334 2.065566876239082E-4 74-75 0.0012806514632682307 0.0 0.0 0.5633420541566848 2.065566876239082E-4 76-77 0.0012806514632682307 0.0 0.0 0.650612254677786 2.065566876239082E-4 78-79 0.001363274138317794 0.0 0.0 0.7298474000503172 2.065566876239082E-4 80-81 0.0018176988510903921 0.0 0.0 0.8369057312457888 2.065566876239082E-4 82-83 0.0018590101886151737 0.0 0.0 0.9642685848346906 2.065566876239082E-4 84-85 0.0018796658573775645 0.0 0.0 1.1027648438865212 2.065566876239082E-4 86-87 0.0019003215261399553 0.0 0.0 1.315229052776473 2.065566876239082E-4 88-89 0.0019003215261399553 0.0 0.0 1.5704091846670492 2.065566876239082E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4830 0.0 48.39848 1 GTATCAA 8490 0.0 40.01401 1 ATCAACG 10915 0.0 30.764997 3 TATCAAC 11080 0.0 30.480211 2 TCAACGC 11060 0.0 30.358522 4 CAACGCA 11320 0.0 29.661242 5 AACGCAG 11545 0.0 29.123713 6 ACGCAGA 13305 0.0 25.057032 7 CGCAGAG 13485 0.0 24.616913 8 GTACATG 8175 0.0 24.526663 1 TACATGG 8520 0.0 22.913443 2 ACATGGG 8340 0.0 21.982773 3 GCAGAGT 14900 0.0 21.769167 9 GAGTACT 9115 0.0 21.207087 12-13 GTGGTAT 2715 0.0 20.12527 1 TGGTATC 2570 0.0 19.960838 2 AGTACTT 9545 0.0 19.779005 12-13 AGAGTAC 13510 0.0 19.614904 10-11 CAGAGTA 14170 0.0 19.036446 10-11 GTACTTT 10070 0.0 19.008999 14-15 >>END_MODULE